• Nuclear Engineering and Technology
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• v.40 no.7
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• pp.551-560
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• 2008

Radiation has stochastic aspects in its generation, its choice of interaction mode during traveling in media, and its impact on living bodies. In certain circumstances, like in high dose environments resulting from low-LET radiation, the variance in its impact on a target volume is negligible. On the contrary, in low dose environments, especially when they are attributed to high-LET radiation, the impact on the target carries with it a large variance. This variation is more significant for smaller target volumes. Microdosimetric techniques, which have been developed to estimate the distribution of radiation energy deposited to cellular and subcellular-sized targets, contrast with macrodosimetric techniques which count only the average value. Since cells and DNA compounds are the critical targets in human bodies, microdosimetry, or dose estimation by microscopic approach, helps one better analyze the biological effects of radiation on the human body. By utilizing microbeam systems designed for individual cell irradiation, scientists have discovered that human cells exhibit radiosensitive reactions without being hit themselves (bystander effect). During the past 10 or more years, a new therapeutic protocol using discontinuous multiple micro-slit beams has been investigated for its clinical application. It has been suggested that the beneficial bystander effect is the essence of this protocol.

• Animal cells and systems
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• v.4 no.3
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• pp.267-272
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• 2000

Partial sequence of the mitochondrial cytochrome b gene was analyzed to investigate genetic variation from 10 geographic populations of Granulilittorina exigua in Korea. The sequence of 282 base pairs was determined by PCR-directed silver sequencing method. The sequences of two species within the genus Littorina reserved in NIH blast search were utilized to determine geographic variations of species referred. The levels of mtDNA sequence differences were 0.00-2.54% within populations and 0.71-4.43% between populations. There were four amino acid differences between representative species of the genera Granulilittorina and Littorina, but no differences within populations of the genus Granulilittorina. The UPGMA and the N-J trees based on Tamura-Nei genetic distance matrix were constructed, which showed that the genus Granulilittorina was divided into three groups such as eastern (even exception for Tokdo population), southern, and western regional populations. The degrees of genetic divergence within populations of each group were p=0.021, p=0.019, and p=0.018, respectively. The divergence between the eastern and southern populations was p=0.032, showing closer relationship than with the western populations (p=0.052). Based on the diverged time estimation, the eastern and southern populations of Granulilittorina exigua in Korea diverged from the western populations about 2.1 MYBP, and the eastern and southern populations diverged from each other about 1.3 MYBP.

• Journal of Biomedical Engineering Research
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• v.27 no.3
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• pp.83-88
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• 2006

A new method of measuring pulse arrival time (PAT), which is usually used for the estimation of systolic blood pressure, in an unconstrained manner using a chair, is proposed. The capacitive-coupled ECG (CC-ECG) measurement system and the air cushion with balancing tubes system were used for unconstrained PAT measurement. Firstly, the correlation between the standard PAT (S-PAT) from the photoplethysmography (PPG) and the PAT measured in an unconstrained manner (U-PAT) was evaluated. It was observed that U-PAT, which is the time delay from the R-peak of ECG to the steepest decent point of air cushion pressure wave, is significantly correlated with the S-PAT. Secondly, systolic blood pressure (SBP) measured by the radial tonometer is compared to the U-PAT. The ten-beat averaged U-PAT removed respiration effects and demonstrated a high intra-subject correlation with SBP in all participants. Finally, the tonometry SBP was estimated from these U-PAT values for one participant intermittently during half a day.

• BMB Reports
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• v.29 no.6
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• pp.540-544
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• 1996

Ferritins from germinated pumpkin seeds were isolated by ammonium sulfate precipitation (0.55 saturation), ion-exchange chromatography on DEAE-cellulose, and gel filtration chromatographies on Sephacryl S-300 and Sephadex G-100. Pumpkin ferritin contains less iron than soybean ferritin. Pumpkin ferritin cross-reacted with anti-soybean ferritin antiserum made in rabbit, and showed two distinct antibody reactive bands, both of equal intensity. The pumpkin ferritins corresponding to the two bands were separable by centrifugation in a sucrose gradient (20~50%). The molecular weights of the native pumpkin ferritins based on the estimation of sucrose gradient centrifugation, gel filtration on Sephacryl S-300 and non-denaturing polyacrylamide gel electrophoresis appeared to be: 530~580 KD (the large molecular weight pumpkin ferritin) and 330-360 KD (the small molecular weight pumpkin ferritin) The large molecular weight pumpkin ferritin contains less iron. Both pumpkin ferritins cross-reacted with anti-soybean ferritin antibody with a spur formation suggesting partial antigenic recognition.

• Journal of Korean Biological Nursing Science
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• v.8 no.2
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• pp.41-59
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• 2006

Chronic liver diseases and hepatic cancer have been reported as 10% of cause of death in Koreans. Regardless of various causes, chronic liver disease accompanies commonly hepatic fibrosis. But still the mechanism of hepatic fibrosis remains poorly understood. Using the dimethylnitrosamine(DMN)-induced hepatic fibrosis rat model, We performed to evaluate the possible therapeutic effect of RIP(extracts of Phellodendron amurense and Patrinia scabiosaefolia) and to investigate the changes in referential connective tissue proteins($TGF-{\beta}_1$, ${\alpha}$-smooth muscle actin, and vimentin) as a marker of fibrogenesis. For these purposes, liver tissues were stained with H & E, and Azan staining for estimation of developing fibrosis. In the DMN-treated rat liver tissue, fibrosis were developed forming incomplete septal fibrosis. Whereas, in the RIP-treated rat liver tissues, the fibrosis were decreased recovering to normal morphology. The expressions of $TGF-{\beta}_1$, ${\alpha}$-smooth muscle actin($\alpha-SMA$), and vimetin were increased in the DMN-treated rat liver tissues, but decreased in the various areas of RIP-treated rat liver tissues. According to these results, RIP could be a possible therapeutic agent to reduce hepatic fibrosis, and the $TGF-{\beta}_1$, ${\alpha}$-SMA, and vimentin could be possible indicative markers of hepatic fibrosis development and recovery.

• Proceedings of the KOSOMBE Conference
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• v.1996 no.05
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• pp.279-282
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• 1996

Sequential brain SPECT imaging has been used to assess the cerebral perfusion reserve(CPR) in cerebrovascular diseases(CVD). We have realized a parametric images of CPR using normalized difference ratio and deadtime correction. For the anatomical localization of CPR, the parametric images were registered to the contours of the cerebral regions using optimal threshold method. The parametric imags reflected the CPR more reliably and distinctively. We conclude that the qunatitative estimation of CPR using normalized difference ratio image could be useful for the diagnosis and assessment of postoperative prognosis in CVD.

• Water Engineering Research
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• v.4 no.1
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• pp.19-30
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• 2003

TMDL development and implementation have great potential fur use in efforts to improve water quality management, but the TMDL approach still has several difficulties to overcome in terms of cost, time requirements, and suitable methodologies. A well-defined prioritization approach for identifying watersheds of concern among several tar-get locations that would benefit from TMDL development and implementation, based on a simple screening approach, could be a major step in solving some of these difficulties. Therefore, a web-based decision support system (DSS) was developed to help identify areas within watersheds that might be priority areas for TMDL development. The DSS includes a graphical user interface based on the HTML protocol, hydrological models, databases, and geographic information system (GIS) capabilities. The DSS has a hydrological model that can estimate non-point source pollution loading based on over 30 years of daily direct runoff using the curve number method and pollutant event mean concentration data. The DSS provides comprehensive output analysis tools using charts and tables, and also provides probability analysis and best management practice cost estimation. In conclusion, the DSS is a simple, affordable tool for the preliminary study of TMDL development via the Internet, and the DSS web site can also be used as an information web server for education related to TMDL.

• Journal of Bio-Environment Control
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• v.1 no.1
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• pp.1-13
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• 1992

This study was attemped to provide some fundamental data for the safety structural design of biological production facility. Wind speed and snow depth according to recurrence intervals for design load estimation were calculated by frequency analysis using the weather data of 60 stations in Korea. The following results were obtained : 1. Type-I extremal distribution was selected for the probability density function of yearly maximum wind speed and snow depth and result of Chi-square goodness of fit showed highly significance at most regions. 2. Design frequency factors for given number of samples and recurrence intervals were calculated, and also design wind speed and snow depth as shown in Table 5-Table 6 and Fig.3-Fig.4 were derived. 3. About 46.4% of the winds having maximum wind speed at every station was analyzed to be same direction, and the consideration of this fact may improve the structural safety. 4. Considering wind speed and snow depth, protected cultivation is very difficult in Ullungdo and the Youngdong districts, and strong structural design is needed in the Chungnam and Junbuk west seaside against snow depth and the west-south seaside against wind speed in Korea.

• The Korean Journal of Pesticide Science
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• v.16 no.2
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• pp.137-144
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• 2012

In order to monitor the pesticide residues in rice paddy soils and paddy waters in Korea and evaluate leachabilities to groundwater of the pesticides detected, paddy soils and paddy waters were collected twice from 40 sites of rice growing paddy fields in July and August in 2011, respectively. Pesticide residues in the samples were analyzed by multi-residue method with GC and HPLC. Five pesticides, such as butachlor, cypermethrin, iprobenfos, oxadiazon, and pendimethalin were detected from thirteen paddy soils and detection rate was about 16.3%, while no pesticide was detected in paddy water. As a results of the estimation of leachabilities for the pesticides detected using GUS (groundwater ubiquity score), their GUSs were less than 1.3, representing they have no potentials to leach into groundwater.

• Journal of Biosystems Engineering
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• v.28 no.4
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• pp.343-350
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• 2003

This study was conducted to find out the coefficient relationships between intensity values of image processing and biological/physical parameters of soil in greenhouses. Soil images were obtained by an image processing system consisting of a personal computer and a CCD earners. A software written in Visual C$\^$++/ systematically integrated the functions of image capture, image processing, and image analysis. Image processing data of the soil samples were analyzed by the method of regression analysis. The results are as follows. For detecting soil density of unbroken soil samples, the highest correlation coefficients of 0.82 and 0.84, respectively were obtained fur R-value and S-value among image processing data while it was 0.97 for G-value. Considering the relationship between biological characteristics and image processing data of soil in greenhouse, the correlation was found generally low. For pH of unbroken soil sample, the correlation coefficients were found 0.87, 0.85, and 0.94, respectively with G, I, and H values of image processing data. In the case of bacteria, any correlation was not found with the image processing data For Actinomyctes, they were 0.86 and 0.85, respectively with G-value and B-value of image processing data showing high correlation coefficient compared to the other variables. The correlation coefficient between Fungi and H-value was shown 0.88, the highest among the variables higher than 0.8 while the other variables showed low correlation. For broken soil samples from greenhouse, the relation between biological parameter and image processing data were rarely shown in this study. The results of this study indicated that most of correlation coefficient between the variables were usually lower than 0.01. Accordingly, it was assumed that the soil should be used without broken to fairly estimate biological characteristics using CCD camera.