• KSBB Journal
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• v.13 no.4
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• pp.461-467
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• 1998

A fermentation system was supplemented with a device for the measurement of the durations of alkali pump feeding for automatic pH control and an A/D convertor for precise monitoring of pH value by computer. A software program was developed to measure buffer capacities from the pH signal and the pH control signal during fermentation. By measuring the buffer capacity on-line, levels of acetic acid were estimated by a software sensor using pH signal in a fermentation process of E.coli growing in a minimal medium. The measured values of acetic acid showed correlation to those of estimated by the software sensor. Lacitic acid production was also successfully estimated by the values of buffer capacities measured on-line.

• Asian-Australasian Journal of Animal Sciences
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• v.4 no.1
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• pp.1-5
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• 1991

Responsiveness of the testis to pregnant mare's serum gonadotropin (PMSG) was studied in immature Nili-Ravi buffalo bulls. Four month old bull calves weighing between 66 to 100 kg raised under uniform condition, were treated with 1000 IU PMSG or vehicle, daily, for six days. PMSG induced an increase in the size of the testis, enlargement of the seminiferous tubules and activation of the spermatogonia. The number of differentiated Leydig cells in the testis of gonadotropin treated animals increased considerably over that of the control testes. A significant increase in plasma testosterone concentrations was observed 24 h following the first injection of PMSG and the levels continued to increase until day 6. In vehicle treated animals plasma testosterone levels remained more or less at pretreatment values. The data suggest that buffalo bull testis is functionally responsive to gonadotropin at an early stage of prepubertal development.

• Korean Journal of Ecology and Environment
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• v.55 no.4
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• pp.415-420
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• 2022

It is essential that continual assessments of the impact of mine-derived water as a long-lasting burden on freshwater environments. Abundance-based evaluations of benthic macroinvertebrates have been conducted to evaluate anthropogenic disturbances and devise policies to reduce their impact. In this study, the status of a stream habitat was evaluated based on the body length and biomass weight of benthic macroinvertebrates of the family Baetidae. Following the renewal of the mining water treatment plant, the abundance of Baetidae assemblages recovered to a level comparable to that of a reference site. However, relatively low values were found for both body length and biomass weight in Baetidae species inhabiting the reddened streambed area, suggesting that the habitat has not yet been completely recovered despite the recovery of the abundance of the Baetidae assemblages. Therefore, continuous investigation and evaluation of this disturbed stream are necessary until their growth conditions of the habitat have functionally recovered.

• Journal of Korean Biological Nursing Science
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• v.2 no.1
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• pp.20-33
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• 2000

The author was used for the ICR mouse and induced diabetes with the streptozotocin(50mg/kg)and alloxan(40mg/kg). After the testing and the identifying the diabetes, the histological changes of the glomerulus, blood test for the values of blood sugar, and urine test for the values of urine protein were investigated. The results are as follows : 1. The values of high blood sugar appeared from the 2 group were about $378mg/d{\ell}{\sim}709mg/d{\ell}$, in the treated groups with the streptozotocin and alloxan. The glycosuria were obviously continued from the 2 weeks to the 12 weeks of the streptozotocin and the alloxan treated groups and the proteinuria was ${\pm}{\sim}+$ in the 4 weeks and 8 weeks of streptozotocin treated group and were + in all the 12 weeks. The ketonuria were generally negative. 2. In the view of the light microscope, there was no significant histological changes until the 8 weeks. However, in the 12 weeks group treated with the streptozotocin, the mesangial matrix of glomerulus increased Bowman's capsules adhered to each other and changed them to the crescence shapes because of increasing the exothelial cells.

• Journal of Korean Biological Nursing Science
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• v.21 no.4
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• pp.275-282
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• 2019

Purpose: This study aimed to identify the differences in blood pressure among adults in the community according to age and time of the blood pressure measurement. Methods: This was a secondary analysis study, using data from a 2015 community health survey, conducted by the Korea Centers for Disease Control and Prevention. The data of three-time-measured blood pressure were collected from 337 subjects, 25, 35, 45, 55, 65, 75 years old, which are median ages by each age group. Results: The primary systolic pressure was significantly higher than the secondary systolic pressure (t= 3.46, p= .001) and the tertiary systolic pressure (t= 4.83, p= .001). The secondary systolic pressure was higher than the tertiary measurement (t= 2.05, p= .041). There was no significant difference between the three-time-measured values for diastolic pressure. There was a significant interaction between measurement times and age in the systoic blood pressure readings (F= 1.95, p= .036). However, there was no significant interaction between measurement times and age in the diastolic blood pressure readings (F= 1.03, p= .418). Conclusion: The findings suggest that attention must be paid to the use of blood pressure values in studies or one-time-measured clinical blood pressure values. In particular, the differences in systolic pressure readings taken at different times in the older age groups were significant. Therefore, it is more important to carefully assess blood pressure in adults over the age of 45 compared to other age groups.

• Membrane and Water Treatment
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• v.14 no.3
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• pp.115-120
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• 2023

The design theory for nanoporous filtration membranes needs to be established. The present study shows that the performance and technical advancement of nanoporous filtration membranes are determined by the fundamental parameter I (in the unit Watt^1/2) which is formulated as a function of the shear strength of the liquid-pore wall interface, the radius of the filtration pore, the membrane thickness, and the bulk dynamic viscosity of the flowing liquid. This parameter determines the critical power loss on a single filtration pore for initiating the wall slippage, which is important for the flux of the membrane. It also relates the membrane permeability to the power cost by the filtration pore. It is shown that for biological cellular membranes its values are on the scale 1.0E-8Watt^1/2, for mono-layer graphene membranes its values are on the scale 1.0E-9Watt^1/2, and for nanoporous membranes made of silica, silicon nitride or silicon carbonized its values are on the scale 1.0E-5Watt^1/2. The scale of the value of this parameter directly measures the level of the performance of a nanoporous filtration membrane. The carbon nanotube membrane has the similar performance with biological cellular membranes, as it also has the value of I on the scale 1.0E-8Watt^1/2.

• Journal of Applied Biological Chemistry
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• v.66
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• pp.81-89
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• 2023

The purpose of this study is to demonstrate the potential enhancement of the flavonoid contents from Agastache rugosa, which can be obtained as raw materials for functional products in the food medicine industry by identifying important factors for efficient preparation to save costs and time in terms of economic factors. For this reason, response surface methodology using Box-Behnken design was used to optimize the extraction conditions for the maximum yield of seven major compounds from A. rugosa. The optimum conditions were obtained with an ethanol concentration of 60.0%, a temperature of 50 ℃, and an extraction time of 33.6 min, meaning that the regression analysis fits the experimental data well. Under these conditions, the seven major compounds 1-7 had observed values of 2.169, 2.135, 0.697, 2.485, 0.105, 1.247, and 0.551%, respectively. These results show that the observed values are in good agreement with the predicted values in the regression model. This process for optimization study exhibited a basic protocol for obtaining stable ingredients from A. rugosa that are appropriate for the development of effective functional products.

• Journal of Microbiology and Biotechnology
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• v.33 no.10
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• pp.1292-1298
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• 2023

PAMB 00755^T, a bacterial strain, was isolated from Korean fir leaves. The strain exhibits yellow colonies and consists of Gram-negative, non-motile, short rods or ovoid-shaped cells. It displays optimal growth conditions at 20℃, 0% NaCl, and pH 6.0. Results of 16S rRNA gene-based phylogenetic analyses showed that strain PAMB 00755^T was most closely related to Sphingomonas chungangi MAH-6^T (97.7%) and Sphingomonas polyaromaticivorans B2-7^T (97.4%), and ≤96.5% sequence similarity to other members of the genus Sphingomonas. The values of average nucleotide identity (79.9-81.3%), average amino acid identity (73.3-75.9%), and digital DNA-DNA hybridization (73.3-75.9%) were significantly lower than the threshold values for species boundaries; these overall genome-related indexes (OGRI) analyses indicated that the strain represents a novel species. Genomic analysis revealed that the strain has a 4.4-Mbp genome encoding 4,083 functional genes, while the DNA G+C content of the whole genome is 66.1%. The genome of strain PAMB 00755^T showed a putative carotenoid biosynthetic cluster responsible for its antioxidant activity. The respiratory quinone was identified as ubiquinone 10 (Q-10), while the major fatty acids in the profile were identified as C_18:1ω7c and/or C_18:1ω6c (summed feature 8). The major polar lipids of strain PAMB 00755^T were diphosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid, and phosphatidylcholine. Based on a comprehensive analysis of genomic, phenotypic, and chemotaxonomic characteristics, we proposed the name Sphingomonas abietis sp. nov. for this novel species, with PAMB 00755^T as the type strain (= KCTC 92781^T = GDMCC 1.3779^T).

• Applied Biological Chemistry
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• v.41 no.4
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• pp.273-274
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• 1998

In vitro cytotoxicity of sambutoxin was measured by using various human and murine tumor cells and $IC_{50}$ values of sambutoxin ranged from 46.2 to 1,425.6 ng/ml.

• Journal of Applied Biological Chemistry
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• v.57 no.2
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• pp.159-160
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• 2014

An antifungal antibiotic was extracted three times using n-butanol from the culture broth of Bacillus sp. Bioassayguided column chromatography with silica gel and Sephadex LH-20 yielded 62 mg of the original active compound from 1 L of culture broth. The minimal inhibitory concentration values were 25 and $50{\mu}g/ml$ against Pyricularia oryzae and Pellicularia filamentosa, respectively. Based on results obtained from the analysis of the structure of the antibiotic using MS, NMR, and IR spectroscopy, the antifungal antibiotic was shown to consist of only six of fructose.