• Journal of Microbiology and Biotechnology
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• v.31 no.1
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• pp.104-114
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• 2021

Petroleum-contaminated soil is considered among the most important potential anthropogenic atmospheric methane sources. Additionally, various rhizoremediation factors can affect methane emissions by altering soil ecosystem carbon cycles. Nonetheless, greenhouse gas emissions from soil have not been given due importance as a potentially relevant parameter in rhizoremediation techniques. Therefore, in this study we sought to investigate the effects of different plant and soil amendments on both remediation efficiencies and methane emission characteristics in diesel-contaminated soil. An indoor pot experiment consisting of three plant treatments (control, maize, tall fescue) and two soil amendments (chemical nutrient, compost) was performed for 95 days. Total petroleum hydrocarbon (TPH) removal efficiency, dehydrogenase activity, and alkB (i.e., an alkane compound-degrading enzyme) gene abundance were the highest in the tall fescue and maize soil system amended with compost. Compost addition enhanced both the overall remediation efficiencies, as well as pmoA (i.e., a methane-oxidizing enzyme) gene abundance in soils. Moreover, the potential methane emission of diesel-contaminated soil was relatively low when maize was introduced to the soil system. After microbial community analysis, various TPH-degrading microorganisms (Nocardioides, Marinobacter, Immitisolibacter, Acinetobacter, Kocuria, Mycobacterium, Pseudomonas, Alcanivorax) and methane-oxidizing microorganisms (Methylocapsa, Methylosarcina) were observed in the rhizosphere soil. The effects of major rhizoremediation factors on soil remediation efficiency and greenhouse gas emissions discussed herein are expected to contribute to the development of sustainable biological remediation technologies in response to global climate change.

• Journal of environmental and Sanitary engineering
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• v.17 no.3
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• pp.52-59
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• 2002

Pilot-scale PSSBR(Phase Separated Sequencing Batch Reactor) was operated to evaluate requirement of external carbon sources(${\Delta}gCOD/{\Delta}gNO_3^{-}-N$) in denitrification. Methanol and fermented food waste were used as external carbon sources. Methanol and fermented food waste were fed to the anoxic state of first reactor and concentration were 50 and 40 mgCOD/L on the basis of concentration in reactor, respectively. In case that external carbon source was not used, average $NO_3^{-}-N$ concentration in effluent was 22.49 mg/L. When methanol and fermented food waste were fed, average $NO_3^{-}-N$ concentration in effluent were 10.13 mg/L and 6.3 mg/L, respectively and requirement of external carbon sources were 4.04 and 2.5 ${\Delta}gCOD/{\Delta}gNO_3^{-}-N$, respectively. Fermented food waste was better than methanol in denitrification efficiency. Therefore fermented food waste could be one of the excellent external carbon sources for nitrogen removal in biological nutrient removal process.

• Korean Journal of Microbiology
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• v.25 no.4
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• pp.333-338
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• 1987

In order to establish the biological treatment system which can be used for treatment of waste aster from acetaldehyde plant, it was investigated optimum nutrient requirements and growth conditions by mixed culture of Micrococcus roseus AW-6, Micrococcus luteus AW-22, Microbacterium lacticum AW-38 and Microbacterium laevaniformans AW-41 as well as the effect of coagulants and neutralization reagents. Also, it was carried out the continuous culture as well as batch culture to treat the waste water by mixed culture of these strains. The COD removal rate was reached to maximum state for 96hrs culture at pH7.0 and $30^{\circ}C$ NaOH as the neutralization reagents was the most effective, but the coagulants had no effect on the COD remonal rate and the optimum dilution times for treatment were 10 fold. The COD removal rate was also increased by supplimenting 200 ppm $NH_{2}NO_{3}$, 50 ppm $KH_{2}PO_{4}$, 15 ppm $CaCl_{2}$ and 1 ppm $MgSO_{4} \cdot 7H_{2}O $ as additional nutrients. The removal rate coefficient $K_{1}$ on the batch culture was $4.5\times 10^{-6}$, and the detention time for BOD removal rate of 85% was approximately 45hrs. The COD of waste water was reduced to 15% of its initial value by the continuous culture. The COD and BOD of the effluents were to be about 60 ppm and 40 ppm, respectively, and final pH was 7.0.

• Membrane Journal
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• v.28 no.3
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• pp.196-204
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• 2018

The anaerobic fluidized bed bioreactor (AFBR) treating synthetic wastewater to simulate domestic sewage was operated under GAC fluidization to provide high surface area for biofilm formation. Although the AFBR achieves excellent COD removal efficiency due to biological activities, concerns are still made with nutrient such as nitrogen remaining in the effluent produced by AFBR. In this study, forward osmosis membrane was applied to treat the effluent produced by AFBR to investigate removal efficiency of total nitrogen (TN) with respect to the draw solution (DS) such as NaCl and glucose. Permeability of FO membrane increased with increasing DS concentration. About 55% of TN removal efficiency was observed with the FO membrane using 1 M of NaCl of draw solution, but almost complete TN removal efficiency was achieved with 1 M of glucose of draw solution. During 24 h of filtration, there was no permeate flux decline with the FO membrane regardless of draw solution applied.

• Journal of Korean Society of Water and Wastewater
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• v.26 no.3
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• pp.471-478
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• 2012

Where an activated sludge system needs to be converted to biological nutrient removal(BNR) system, the secondary clarifier must handle higher MLSS from bioreactor since nitrification in BNR system that requires higher SRTs than activated sludge system. Either increase the clarifier size or modification of clarifier physical structure is required to cope with MLSS surge. One of recommended structural modification is the insertion of Lamellar within clarifier. In this study, two clarifiers - one has Lamellar structure inserted and the other does not - were used to compare the effect of Lamellar in solid/liquid separation. Same MLSS was fed to both clarifiers and concentrations of MLSS were varied. With all MLSS concentrations, attachment of MLSS on Lamellar was observed and it was found that detached MLSS caused the higher effluent SS concentrations than that of non-Lamellar clarifier effluent. From these results, Lamellar should not be inserted in clarifier to handle MLSS from BNR processes and the recommendation must be withdrawn.

• Journal of Environmental Health Sciences
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• v.27 no.4
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• pp.79-83
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• 2001

The sludge wastes fermentation process reactors were operated to produce the VFAs(volatile fatty acids) as supplemental carbon sources and to determine the optimum operating conditions. The experiment was carried out by varied mixture ration of 400:0 350:30 300:100 200:200 and operating temperature 2$0^{\circ}C$ 3$0^{\circ}C$ and 4$0^{\circ}C$ The results were as follows: Higher VFAs production rate observed at higher mixed ratio of primary sludge. When the mixed ratio of primary sludge and return sludge were 400:0 350:50 300:100 200:200 respectively. VFAs production are were 829.6mg/l 944.2 mg/l 597.9mg/ml an d441.6 mg/l , respectively. the yield of VFAs increased with temperature, but decreased with initial TSS concentration Because fermented sludge has relatively low nitrogen and phosphorus and relatively high VFAs it can be used as a substitute for external carbon in biological nutrient removal process.

• Journal of Environmental Health Sciences
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• v.29 no.3
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• pp.16-20
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• 2003

Nitrogen(N) and phosphorus(P) in surface streams mainly lead to euthrophication. It aggravates water quality and consequently increases the purification costs. As a resolution of water contamination caused by household drainage through irrigation route by 70% of the 1,300 community residents in Eum-Am Myun, Seo-San city, was implemented biological self-purification method by growing Oenanthe Javanica along the polluted water tunnel. The contaminated water was efficiently purified after passing the dropwort field; DO conc. of effluent water was increased 8.3∼61.9% after through the drop wort field. HRT of experiment system was changed 0.05∼1.50/day. 50% of BOD was eliminated at the range above 12 mg/l of Influent BOD conc. Also, 50% of COD was eliminated at the range above 30 mg/l of Influent COD conc. Finnally, the influent T-N loading at range below 1.5 g/m$^3$/d reduced 50% of Influent T-N conc., and so did influent T-P loading at the range below 0.03 g/m$^3$/dwas reduced 50% of Influent T-P conc.

• Proceedings of the Korea Society of Environmental Biology Conference
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• 1994.05a
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• pp.15-36
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• 1994

Biological techniques by aquatic macroanimals to improve water quality may be classified overally as three types. The fist is to use aquatic palnts for nutrient removal, the second is to use grass fish to control algae or aquatic plant, and the third is to modify trophostructure by using top-consumer to control algae(top-down effect). This paper includes a general review on above-mentioned techniques, some experimental or field-based results, and some discussion on Korean status and prospect on this part.

• Journal of Environmental Science International
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• v.28 no.10
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• pp.899-905
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• 2019

Recently, an innovative method for wastewater treatment and nutrient removal was developed by combining the sequence batch reactor and membrane bioreactor to overcome pollution caused by shipboard sewage. This system is a modified form of the activated sludge process and involves repeated cycles of mixing and aeration. In the present study, the bacterial diversity and dominant microbial community in this wastewater treatment system were studied using the MACROGEN next generation sequencing technique. A high diversity of bacteria was observed in anaerobic and aerobic bioreactors, with approximately 486 species. Microbial diversity and the presence of beneficial species are crucial for an effective biological shipboard wastewater treatment system. The Arcobacter genus was dominant in the anaerobic tank, which mainly contained Arcobacter lanthieri (8.24%), followed by Acinetobacter jahnsonii (5.81%). However, the dominant bacterial species in the aerobic bioreactor were Terrimonas lutea (7.24%) and Rubrivivax gelatinosus (4.95%).

• Journal of Korean Society of Water and Wastewater
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• v.21 no.3
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• pp.295-303
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• 2007

Nitrogen compounds in municipal wastewater can be divided into biodegradable and nonbiodegradable fractions with biodegradability. Biodegradable nitrogen compounds can be removed through biological nitrification and denitrification processes, and nonbiodegradable nitrogen compounds affect the effluent quality of biological nutrient removal processes. The amount of nitrifiable nitrogen compounds, which are the sum of ammonia and biodegradable organic nitrogen, has been estimated by respirometry. Respirometry shows good estimation of the concentration of nitrifiable nitrogen when a synthetic sample of ammonium chloride is dosed. The estimated concentration of nitrifiable nitrogen compounds in municipal wastewater is close to ammonia concentration in municipal wastewater, but it is lower than that for the synthetic sample. If nitrogen assimilated into cell synthesis of nitrifiers and heterotrophs is considered, the total amounts of nitrifiable nitrogen compounds, which are nitrified and assimilated, could be more accurately estimated. The concentration of nitrifiable nitrogen compounds, which are biodegradable, is about 31 mg N/l, and this is 119% of ammonia and 94% of total nitrogen. Ammonia, nitrate, biodegradable organic nitrogen, and nonbiodegradable nitrogen are about 79%, 1%, 15%, and 5% of the total nitrogen in municipal wastewater, respectively.