• Title/Summary/Keyword: Biological Engineering

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Synthesis and Properties of Pyrrolidinium and Piperidinium Bis(trifluoromethanesulfonyl)imide Ionic Liquids with Allyl Substituents

  • Yim, Tae-Eun;Lee, Hyun-Yeong;Kim, Hyo-Jin;Mun, Jun-Young;Kim, Sang-Mi;Oh, Seung-M.;Kim, Young-Gyu
    • Bulletin of the Korean Chemical Society
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    • v.28 no.9
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    • pp.1567-1572
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    • 2007
  • New pyrrolidinium and piperidinium bis(trifluoromethanesulfonyl)imide (TFSI) ionic liquids (ILs) having allyl substituents were synthesized and characterized. All of them are liquid at room temperature and stable up to 300 oC. The pyrrolidinium-based ILs showed better conductivities and lower viscosities than the corresponding piperidinium-based ILs. Among them, 1-allyl-1-methylpyrrolidinium TFSI showed the lowest viscosity of 52 cP, the highest conductivity of 5.7 mS cm?1, and the most negative cathodic voltage window of ?3.2 V (vs. Fc/Fc+) on a platinum electrode, which are the improved results compared to the corresponding analogue having a saturated substituent, 1-methyl-1-propylpyrrolidinium TFSI.

Investigation of cultivation and FAME composition isolated Phaeodactylum tricornutum from Youngheung island (영흥도에서 분리된 Phaeodactylum tricornutum의 증식 및 Monounsaturated fatty acid 관련 지방산 조성 분석)

  • Lee, SangMin;Cho, Yonghee;Shin, ong-Woo;Jeon, Hyonam;Ryu, YoungJin;Lim, Sang-Min;Lee, Choul-Gyun
    • Journal of Marine Bioscience and Biotechnology
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    • v.6 no.2
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    • pp.47-52
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    • 2014
  • Oxidation stability and cold fuid property are considered as the most important factors for determining biodiesel quality. Among the fatty acids, monounsaturated fatty acid satisfy both oxidation stability and cold flow property of biodiesel quality standards. Microalgae with high monounsaturated fatty acid contents is have some benefit for producing to produce biodiesels with satisfying quality standards. In this study, monounsaturated fatty acid contents of a isolated microalga from Youngheung island was analyzed. Phaeodactylum tricornutum was isolated by streaking, and growth rate and fatty acid composition of the algae were investigated. Total FAME contents were consisted of 26% of saturated fatty acids, 43% of monounsaturated fatty acids, and 18% of polyunsaturated fatty acids. The contents of monounsaturated fatty acid were especially high in the Phaeodactylum species. This result implies that the FAMEs from P. tricornutum may contribute to improve the oxidation stability and cold flow property of biodiesel.

Fabrication of multi-well platform with electrical stimulation for efficient myogenic commitment of C2C12 cells

  • Song, Joohyun;Lee, Eunjee A.;Cha, Seungwoo;Kim, Insun;Choi, Yonghoon;Hwang, Nathaniel S.
    • Biomaterials and Biomechanics in Bioengineering
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    • v.2 no.1
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    • pp.33-45
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    • 2015
  • To engineer tissue-like structures, cells are required to organize themselves into three-dimensional networks that mimic the native tissue micro-architecture. Here, we present agarose-based multi-well platform incorporated with electrical stimulation to build skeletal muscle-like tissues in a facile and highly reproducible fashion. Electrical stimulation of C2C12 cells encapsulated in collagen/matrigel hydrogels facilitated the formation 3D muscle tissues. Consequently, we confirmed the transcriptional upregulations of myogenic related genes in the electrical stimulation group compared to non-stimulated control group in our multi-well 3D culture platform. Given the robust fabrication, engineered muscle tissues in multi-well platform may find their use in high-throughput biological studies drug screenings.