• Title/Summary/Keyword: Biological Engineering

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Development of a Rapid and Productive Cell-free Protein Synthesis System

  • Kim, Dong-Myung;Choi, Cha-Yong;Ahn, Jin-Ho;Kim, Tae-Wan;Kim, Nam-Young;Oh, In-Suk;Park, Chang-Gil
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.3
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    • pp.235-239
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    • 2006

  • Due to recent advances in genome sequencing, there has been a dramatic increase in the quantity of genetic information, which has lead to an even greater demand for a faster, more parallel expression system. Therefore, interest in cell-free protein synthesis, as an alternative method for high-throughput gene expression, has been revived. In contrast to in vivo gene expression methods, cell-free protein synthesis provides a completely open system for direct access to the reaction conditions. We have developed an efficient cell-free protein synthesis system by optimizing the energy source and S30 extract. Under the optimized conditions, approximately $650{\mu}g/mL$ of protein was produced after 2h of incubation, with the developed system further modified for the efficient expression of PCR-amplified DNA. When the concentrations of DNA, magnesium, and amino acids were optimized for the production of PCR-based cell-free protein synthesis, the protein yield was comparable to that from the plasmid template.

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A Study on the Biological Remediation Technology for Oil Contaminated Sites (유류로 오염된 토양의 생물학적 처리기법에 관한 연구)

  • Cho Jai-Rip
    • Proceedings of the Korean Society for Quality Management Conference
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    • 2004.04a
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    • pp.307-312
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    • 2004

  • Contamination of industrial sites has happened by a variety compounds. Petroleum hydrocarbons, which are readily biodegradable, are reported principle contaminats in most industrial sites. Therefore, the use of biological processes will be a promising technology for remediation of industrial sites. This paper addresses the possible use of biological processes in remediation of contaminated industrial sites and discusses the background and main streams of the process. The paper also characterizes representative biological systems developed for application.

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