• Journal of Microbiology and Biotechnology
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• v.33 no.10
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• pp.1370-1375
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• 2023

In this study, we aimed to enhance the accumulation of chorismate (CHR) and anthranilate (ANT), key intermediates in the shikimate pathway, by modifying a shikimate over-producing recombinant strain of Corynebacterium glutamicum [19]. To achieve this, we utilized a CRISPR-driven genome engineering approach to compensate for the deletion of shikimate kinase (AroK) as well as ANT synthases (TrpEG) and ANT phosphoribosyltransferase (TrpD). In addition, we inhibited the CHR metabolic pathway to induce CHR accumulation. Further, to optimize the shikimate pathway, we overexpressed feedback inhibition-resistant Escherichia coli AroG and AroH genes, as well as C. glutamicum AroF and AroB genes. We also overexpressed QsuC and substituted shikimate dehydrogenase (AroE). In parallel, we optimized the carbon metabolism pathway by deleting the gntR family transcriptional regulator (IolR) and overexpressing polyphosphate/ATP-dependent glucokinase (PpgK) and glucose kinase (Glk). Moreover, acetate kinase (Ack) and phosphotransacetylase (Pta) were eliminated. Through our CRISPR-driven genome re-design approach, we successfully generated C. glutamicum cell factories capable of producing up to 0.48 g/l and 0.9 g/l of CHR and ANT in 1.3 ml miniature culture systems, respectively. These findings highlight the efficacy of our rational cell factory design strategy in C. glutamicum, which provides a robust platform technology for developing high-producing strains that synthesize valuable aromatic compounds, particularly those derived from the shikimate pathway metabolites.

• Journal of Magnetics
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• v.19 no.1
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• pp.55-58
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• 2014

We have synthesized ferromagnetic ${\tau}$-phase $Mn_{54}Al_{46}/{\alpha}$-phase $Fe_{65}Co_{35}$ composite by annealing a mixture of paramagnetic ${\varepsilon}$-phase $Mn_{54}Al_{46}$ and ferromagnetic ${\alpha}$-phase $Fe_{65}Co_{35}$ particles at $650^{\circ}C$. The volume fraction ($f_h$) of hard ${\tau}$-phase $Mn_{54}Al_{46}$ of the composite was varied from 0 to 1. During the annealing, magnetic phase transformation occurred from paramagnetic ${\varepsilon}$-phase to ferromagnetic ${\tau}$-phase $Mn_{54}Al_{46}$. The magnetization and coercivity of the composite monotonically decreased and increased, respectively, as the $f_h$ increased. These results are in good agreement with our proposed composition dependent coercivity and modified magnetization equations.

• Journal of the Korean Society for Precision Engineering
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• v.32 no.10
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• pp.865-871
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• 2015

This study was conducted in order to develop a finger exoskeleton system using ionic polymer metal composites (IPMCs) as the actuator and sensor in a hybrid structure. To use the IPMC as an actuator producing large force, a first order transfer function was obtained using results from a block force for DC excitation that applied to two IPMCs of 20mm-width, 50mm-length, and 2.4mm thickness together. After which the validation of 200gf control with anti-windup PI controller was confirmed. A 5mm-width, 50mm-length, 0.6mm-thickness of IPMC was also modeled as a sensor for tip displacement. As a result, the IPMC sensor could been utilized as a trigger role for the actuator. Finally, an IPMC sensor and actuator were installed on the joint of a single DOF exoskeleton in the hybrid structure, and test for the control of 40gf of block force and predefined sequence of motion was performed.

• Proceedings of the KOSOMBE Conference
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• v.1997 no.05
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• pp.47-50
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• 1997

We synthesized N-Hydroxysuccinimidyl (NHS) azidobenzoate as a cross-linking reagent for immobilization of peptide onto the solid surface. Thin polystyrene(PS) films spin-coated with a NHS azidobenzoate solution were exposed with ultraviolet light at 245nm$(3.3mW/cm^2)$ for 5 min. The NHS active ester groups became covalently attached to the polymer via photogenerated, highly reactive nitrene intermediates derived from NHS azidobenzoate. Using this technique, it is demonstrated that well-defined surface regions can be functionalized with a minimum observable feature size of 1mm for UV exposure. Through reaction of this functionalized PS surface with primary amine-containing biomolecules, biological molecule had been immobilized on the polymer surface.

• Bulletin of the Korean Chemical Society
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• v.35 no.8
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• pp.2381-2384
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• 2014

A solid-phase synthetic approach is reported for the synthesis of an ascorbic acid (ASA)-dipeptide conjugate that exhibited enhanced antioxidant activity. The N-terminal amino group of dipeptide (Ala-Ala) on a resin support was first activated by 1,1'-carbonyldiimidazole (CDI), and then reacted with an ASA derivative. The addition of a base, triethylamine (TEA), promoted nucleophilic acylation of ASA derivative and yielded a desired product (ASA-Ala-Ala) with enhanced purity, when cleaved from the resin. Compared to the approach where a C3 hydroxyl group of ASA was first activated with CDI and then reacted with the amino group of dipeptide on the resin, this new approach allowed a significant reduction of a total reaction time from 120 h to 8 h at $25^{\circ}C$. As-prepared ASA-dipeptide conjugate (ASA-Ala-Ala) showed improved antioxidant activity compared to ASA.

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.318.2-318.2
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• 2014

The high contact resistance is still one of the major issues to be resolved in CdS/CdTe thin film solar cells. CdTe/Metal Schottky contact induced a high contact resistance in CdS/CdTe solar cells. It has been reported that the work function of CdTe thin film is more than 5.7 eV. There has not been a suitable back contact metal, because CdTe thin film has a high work function. In a few decades, some buffer layer was reported to improve a back contact problem. Buffer layers which are Te, $Sb_2Te_3$, $Cu_2Te$, ZnTe:Cu and so on was inserted between CdTe and metal electrode. A formed buffer layers made a tunnel junction. Hole carriers which was excited in CdTe film by light absorption was transported from CdTe to back metal electrode. In this report, we reported the variation of solar cell performance with different buffer layer at the back contact of CdTe thin film solar cell.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2017.05a
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• pp.3-23
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• 2017

The achievement of tissue engineering can be highly depending on the capability to generate complicated, cell seeded three dimensional (3D) micro/nano-structures. So, various fabrication techniques that can be used to precisely design the architecture and topography of scaffolding materials will signify a key aspect of multi-functional tissue engineering. Previous methods for obtaining scaffolds based on top-down are often not satisfactory to produce complex micro/nano-structures due to the lack of control on scaffold architecture, porosity, and cellular interactions. However, a bioprinting method can be used to design sophisticated 3D tissue scaffolds that can be engineered to mimic the tissue architecture using computer aided approach. Also, in recent, the method has been modified and optimized to fabricate scaffolds using various natural biopolymers (collagen, alginate, and chitosan etc.). Variation of the topological structure and polymer concentration allowed tailoring the physical and biological properties of the scaffolds. In this presentation, the 3D bioprinting supplemented with a newly designed plasma treatment for attaining highly bioactive and functional scaffolds for tissue engineering applications will be introduced. Moreover, various in vivo and in vitro results will show that the fabricated scaffolds can carry out their structural and biological functionality.

• Proceedings of the Korean Information Science Society Conference
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• 2005.11b
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• pp.226-228
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• 2005

최근 인터넷을 기반으로 하는 웹의 급속한 발전으로 정보는 인터넷을 통해서 교환되고 공유될 수 있는 개방된 환경에서 더 큰 가치를 지니게 되었다. 이러한 경향은 의료분야에서도 예외 없이 적용되어 지역 네트워크기반의 기존 의료정보 시스템도 타 기관과의 데이터 공유의 필요성으로 인해 개방형 시스템으로의 전환이 요구되고 있다. 따라서, 의료정보의 교환과 공유를 위해 각 의료관련 시스템들에 대한 표준들이 제정되어 적용되고 있다. 그 중 HL7은 임상문서 교환을 위한 유연한 표준이지만 시스템 의존적이거나 이질적인 시스템간의 정보교환에는 부적합하다. 따라서 본 논문은 이질적인 시스템간의 정보교환 및 공유를 위해 바이오 및 임상정보를 통합하고 통합된 정보를 저장할 수 있는 기능을 주로 하는 BioEMR Extractor 를 구현했다. 또한, 이질적인 시스템 통합의 첫 단계로써 RDB기반의 기존 BioEMR 정보를 XML기반의 Data로 변환하는 모듈을 설계 및 구현했다.

• Journal of the Optical Society of Korea
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• v.13 no.3
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• pp.392-397
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• 2009

We designed a wavelength interrogation-based surface plasmon resonance (SPR) biosensor to detect avian influenza DNA (AI-DNA). Hybridization reactions between target AI-DNA probes and capture probes immobilized on a gold surface were monitored quantitatively by measuring the resonance wavelength in the visible waveband. The experimental results were consistent with numerical calculations. Although the SPR detection technique does not require the DNA to be labeled, we also evaluated fluorescently-labeled targets to verify the hybridization behavior of the AI-DNA. Changes in resonance were found to be linearly proportional to the amount of bound analyte. A wavelength interrogation-type SPR biosensor can be used for rapid measurement and high-throughput detection of highly pathogenic AI viruses.

• Journal of Biomedical Engineering Research
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• v.27 no.3
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• pp.83-88
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• 2006

A new method of measuring pulse arrival time (PAT), which is usually used for the estimation of systolic blood pressure, in an unconstrained manner using a chair, is proposed. The capacitive-coupled ECG (CC-ECG) measurement system and the air cushion with balancing tubes system were used for unconstrained PAT measurement. Firstly, the correlation between the standard PAT (S-PAT) from the photoplethysmography (PPG) and the PAT measured in an unconstrained manner (U-PAT) was evaluated. It was observed that U-PAT, which is the time delay from the R-peak of ECG to the steepest decent point of air cushion pressure wave, is significantly correlated with the S-PAT. Secondly, systolic blood pressure (SBP) measured by the radial tonometer is compared to the U-PAT. The ten-beat averaged U-PAT removed respiration effects and demonstrated a high intra-subject correlation with SBP in all participants. Finally, the tonometry SBP was estimated from these U-PAT values for one participant intermittently during half a day.