Proceedings of the KOSOMBE Conference (대한의용생체공학회:학술대회논문집)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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The Covalent Immobilization of Biomolecules to Polymer Surface by Deep-UV Lithography Using N-Hydroxysuccinimidyl Azidobenzoate

  • Kim, H.J. (Institute of Biomedical Engineering, College of Medicine, Seoul National University, Biomedlab. Co.) ;
  • Shim, J.H. (Institute of Biomedical Engineering, College of Medicine, Seoul National University, Biomedlab. Co.) ;
  • Kim, J.H. (Institute of Biomedical Engineering, College of Medicine, Seoul National University, Biomedlab. Co.) ;
  • Kim, J. (Institute of Biomedical Engineering, College of Medicine, Seoul National University, Biomedlab. Co.) ;
  • Min, B.G. (Institute of Biomedical Engineering, College of Medicine, Seoul National University, Biomedlab. Co.)
  • 김현정 (서울대학교 의대 의공학연구소) ;
  • ;
  • ;
  • ;
  • 민병구 (서울대학교)
  • Published : 1997.05.23
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Abstract

We synthesized N-Hydroxysuccinimidyl (NHS) azidobenzoate as a cross-linking reagent for immobilization of peptide onto the solid surface. Thin polystyrene(PS) films spin-coated with a NHS azidobenzoate solution were exposed with ultraviolet light at 245nm$(3.3mW/cm^2)$ for 5 min. The NHS active ester groups became covalently attached to the polymer via photogenerated, highly reactive nitrene intermediates derived from NHS azidobenzoate. Using this technique, it is demonstrated that well-defined surface regions can be functionalized with a minimum observable feature size of 1mm for UV exposure. Through reaction of this functionalized PS surface with primary amine-containing biomolecules, biological molecule had been immobilized on the polymer surface.

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