• Journal of the korean society of oceanography
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• 제36권1호
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• pp.19-26
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• 2001

The biological pump is one of the important pumping mechanisms absorbing CO$_2$ from the atmosphere into the ocean and can be quantified by estimating new production. New production in the open ocean mostly depends on the supply of nitrate from the water below the mixed layer. While nitrate is affected by many biological processes, the helium isotope ($^3$He) is inert and has very simple physical properties. Using the $^3$He flux and the relation between $^3$He and NO${_3}\;{^-}$- within the thermocline, the nitrate flux supporting new production was estimated in the southern East Sea. The average ${\delta}^3$He within the mixed layer was -14$%_o$ and -l5.4$%_o$ in the winter and autumn, respectively. Through the year excess $^3$He occurs in the mixed layer except for a slight depletion of -17$%_o$ in summer. The $^3$He flux of 13$%_o$md$^{-1}$ associated with the concentration gradient at the air-sea interface was calculated from the product of the piston velocity and the excess $^3$He. Tritium decay within the mixed layer could support only 2$%_o$md$^{-1}$ of the flux. Thus, the remaining 11$%_o$md^{-1}$ could be attributed to the flux of tritiugenic $^3$He from the water below the mixed layer. Nitrate and $^3$He were positively correlated within the thermocline layer with the slope of 0.21 ${\mu}$mol kg$^{-1}$ $%_o\;^{-1}$. The annual nitrate flux estimated from the upward flux of $^3$He and the NO$_{3}\;{^-}$-$^3$He relation was 0.8${\pm}$0.2 mol(N) m$^{-2}$yr$^{-1}$. This flux corresponds to an annual new production of 64 g(C) m$^{-2}$yr$^{-1}$, which is consistent with that in the north-west Pacific.

• Animal cells and systems
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• 제7권4호
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• pp.289-294
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• 2003

To quantify nutrient loading from emergent macrophytes through leaching in the littoral zones of Paldang Reservoir, we conducted incubation experiments using leaf litter of the emergent macrophyte, Zizaniz latifolia. To separate the leaching process from microbial decay, we used $HgCl_2$ to suppress microbial activity during the experiment. We measured electric conductivity, absorbance at 280nm, total nitrogen and dissolved inorganic nitrogen, total phosphorus and soluble reactive phosphorus, Na, K, Mg and Ca amounts in leaf litter and in water. In addition, we examined the effects of water temperature and ion concentrations of ambient water on the leaching process. A total of 6% of the initial ash-free dry mass of leaf litter was lost due to leaching during incubation (four days). Electric conductivity and A280 continued to increase and saturate during the incubation. To compare reaching rates of different nutrients, we fitted leaching dynamics with a hyperbolic saturation function [Y=AㆍX/(B+X)]. From these fittings, we found that ratios of leaching amounts to nutrient concentration in the litter were in the order of K > Na > Mg > P > Ca > N. Leaching from leaf litter of Z. latifolia was dependent on water temperature while it was not related with ion concentrations in the ambient water. Our results suggest that the leaching process of nutrients, especially phosphorus, from aquatic macrophytes provides considerable contribution to the eutrophication of the Paldang Reservoir ecosystem.

• 상하수도학회지
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• 제20권5호
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• pp.701-707
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• 2006

Disinfectant residual should be maintained to achieve biological stability during distribution of treated water. The wide distribution of retention times associated with storage and transport of water in a network and the reactivity of disinfectants make it difficult to maintain adequate residuals at critical locations. Rechlorination at some intermediate locations may reduce the total disinfectant dose while keeping residuals within specified limits throughout the water distribution system. In order to select the adequate location of rechlorination for achieving to maintain of residual chlorine throughout the distribution system, EPANET was used in this study. EPANET was well predicted chlorine transport and residual loss in the distribution system. Location of rechlorination was selected to maintain 0.4mg/L of residual chlorine throughout a water distribution system by field investigation and model simulation. The quantity of chlorine may reduced 36.7% and provided smooth residual between 0.42 and 0.60mg/L, when rechlorination would be used continuously at strategic location within the distribution system.

• 한국식품위생안전성학회지
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• 제10권4호
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• pp.263-270
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• 1995

In order to promote the prevention of microbial and enzymatic spoilage and to retain the freshiness, sweet persimmons harvested in Gyeongsangnam-do were treated with graperfruit seed extract(GFSE)-CaCO3 mixture and stored in the proper packaging conditions. A low concentration of GFSE showed effective growth inhibition of plant pathological bacteria and fungi, Enterobacter pyrinus and Fusarium sp., which were involved in the decay of fruits and vegetables. GFSE was stable to heat treatment; its antimicrobial activity was not changed by heat treatment upto 10$0^{\circ}C$. However, when the temperature was raised to 12$0^{\circ}C$, about 90% of total activity was retained within 30 min. GFSE was also highly stable to broad pH changes; its activity was not changed in the range of pH 2.0 to pH 12.0. The physiological function of cell membrane in the spores of Bacillus cereus and the hyphae of Fusarium sp. was destroyed by treating with GFSE. It was observed that treating sweet persimmons with GFSE minxture and storing them in strech-wrapped packages could prolong the greshness of sweet persimmons and reduce quality deterioration.

• Nuclear Engineering and Technology
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• 제48권4호
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• pp.965-974
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• 2016

The article describes the results of experiments conducted on pigs to determine the effect of plutonium, which is the most radiotoxic and highly active element in the range of mixed fuel $(U_{0.8}Pu_{0.2})O_2$ fission products, on living organisms. The results will allow empirical prediction of the emergency plutonium radiation dose for various organs and tissues of humans in case of an accident in a reactor running on mixed fuel $(U_{0.8}Pu_{0.2})O_2$.

• 농약과학회지
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• 제16권4호
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• pp.288-293
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• 2012

실내 재배 오이 중 살균제 amisulbrom의 생물학적 반감기 및 생산단계 잔류허용기준을 제안하고자 약제를 살포하고 재배기간 동안 잔류량을 확인하였다. Amisulbrom 1회 살포시 초기 잔류 농도는 $0.15mg\;kg^{-1}$이었으며 살포 5일 후 $0.06mg\;kg^{-1}$으로 감소되어 반감기는 3.6일이었다. 3일 간격으로 기준량 2회 살포시 초기 잔류량은 $0.35mg\;kg^{-1}$이었으며 살포 5일 후 $0.09mg\;kg^{-1}$으로 감소되어 반감기는 2.4일 이었다. 1회 처리 시의 잔류감소 회귀식을 이용한 생산단계 잔류허용 기준(PHRL)은 수확 5일 전 $1.83mg\;kg^{-1}$ 또는 2일 전 $1.03mg\;kg^{-1}$ 등으로 제안하였다. Amisulbrom 액상 수화제를 안전사용 기준에 따라 1회 살포, 또는 3일 간격 2회 살포 시 모두 살포 직후 잔류량은 오이 중 농약잔류 허용기준 보다 낮았다.

• 한국대기환경학회지
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• 제34권4호
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• pp.554-566
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• 2018

The rice husk contains nutrients which can be easily utilized by microorganisms, and also has a water retaining ability, which played a crucial part in enabling it to become a biofilter media. In this study, we evaluated the applicability of rice husk pellet bio-scrubber as a microbiological carrier. The pelletization experiment of rice husk as a biological media was performed using PVA and EVA binder. Also, the feasibility tests of rice husk as a biological media for odor removal were carried out in order to know whether rice-husk contains useful components as a media for microbiological growth or not. Lastly, a combined test for odor gas absorption and biological oxidation was conducted using a lab scale bio-filter set-up packed with rice-husk pellets as wet-scrubber. The major components of the rice husk were carbon, hydrogen, nitrogen, and oxygen, while carbon acted as the main ingredient which comprised up to 23.00%. The C : N : P ratio was calculated as 45 : 1 : 2. Oxygen uptake rate, yield and decay rate of the rice husk eluent was calculated to be $0.0049mgO_2/L/sec$, 0.24 mgSS/mgCOD and 0.004 respectively. The most stable form of rice husk pellets was produced when the weight of the rice husk, EVAc, PVAc, and distilled water was 10 : 2 : 0.2 : 10. The prepared rice husk pellets had an apparent density of 368 g/L and a porosity of 59.00% upon filling. Dry rice husks showed high adsorption capacity for ammonia gas but low adsorption capacity for hydrogen sulfide. The bio-filter odor removal column filled with rice husk pellets showed more than 99.50% removal efficiency for NH3 and H2S gas. Through the analysis of circulation water, the prime removal mechanism is assumed to be the dissolution by water, microbial nitrification, and sulfation. Finally, it was confirmed that the microorganisms could survive well on the rice husk pellets, which provided them a stable supply of nutrients for their activity in this long-term experiment. This adequate supply of nutrients from the rice husk enabled high removal efficiency by the microorganisms.

• 대한환경공학회지
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• 제28권1호
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• pp.88-96
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• 2006

생물학적 폐수처리과정에서 폐활성슬러지가 적게 발생하도록 하는 방법 중 현재 그 효과가 가장 확실한 것은 반응조 내의 F/M비를 낮게 유지하는 방법이다. 본 연구에서는 반응조 내의 미생물 농도를 높게 유지함으로써 F/M비를 낮게 유지할 수 있는 부직포 여과막 생물반응조에 COD농도가 약 300 mg/L이고 SS를 함유하지 않는 합성폐수를 단속적으로 주입하는 실험을 반복하여 폐활성 슬러지의 감량화 가능성을 파악하였다. 실험 결과, 반응조내의 MLSS농도가 최고 31,010 mg/L까지 증가함으로써 F/M비는 최저 0.02 g COD/g MLSS-day까지 감소하였다. 그러나 MLSS의 내생분해계수 및 산소섭취율을 측정한 결과 MLSS농도가 증가함에 따라 MLSS의 활성도는 감소하는 것으로 나타났다. 폐수주입기간 중 반응조내의 MLSS증가 및 전체 실험기간의 물질수지에 근거한 평균 미생물 성장계수 값이 각각 0.148 및 0.139 g MLSS/g COD의 낮은 값을 보임으로써 본 연구에서 채택된 부직포 여과막 생물반응조가 잉여슬러지 발생량을 감량화시키는 데 효과적임을 알 수 있었다.

• 미생물학회지
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• 제47권1호
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• pp.22-29
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• 2011

Chromophore로 레티날(vitamin A)을 사용하는 막 단백질인 로돕신은 7개의 막 단백질로 이루어진다. 최근 광화학/생물리학 측정 방법이 다양해지면서, 그에 따른 새로운 특성도 함께 다양하게 보고되고 있다. 하지만, 다양한 측정 환경에 따른 그 광화학/생물리학 특성의 차이점에 대한 비교연구는 없는 상태이다. 첫째, 빛 에너지를 이용하여 수소 이온 펌프 역할 하는 것으로 잘 알려져 있는 roteorhodopsin (PR)은 해양 proteobacteria에서 발견 되었다. 둘째, 한 오페론에서 14 kDa 전달자와 같이 발현되면서 신호 전달 기능을 하는 Anabaena sensory rhodopsin (ASR)이 있다. 이에 본 연구에서는 이 두 미생물 로돕신을 이용하여 다양한 조건에서 그 차이를 살펴보았다. 각 단백질이 막에 끼어 있는 상태(membrane state), polyacrylamide gel에 고정되어 있는 상태, nonionic detergent일종인 DDM (n-dodecyl-${\beta}$-D-maltopyranoside)과, OG (octyl-${\beta}$-D-glucopyranoside)에 녹아 있는 상태, 좀더 자연상태와 유사한 환경을 위해 단백질만 깨끗하게 정제한 다음 다시 Escherichia coli의 지질인 DOPC (1,2-didecanoyl-sn-glycero-3-phosphocholine)에 재조립한 상태, 이렇게 5가지 조건에서 각각의 광화학/생물리학 특징을 흡수스펙트럼(absorption spectrum), 빛-어둠 차이 흡수스펙트럼(light-induced difference spectrum), 포토사이클(photocycle)을 측정하였다. 그 결과 DDM에 녹아 있는 PR과 ASR에서 각 다른 파장에서 M과 O state와 같은 선명한 photointermediate를 가지고, 가장 signal/noise 비율이 좋았다. 본 연구를 통해 막단백질의 다양한 측정 환경에 대한 그 특성의 차이점을 살펴봄으로써 앞으로 다양한 종류의 로돕신 연구에 기초 기반이 될 것으로 사료된다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.104.1-104
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• 2003

To screen for potential biocontrol agents against postharvest disease of garlics caused by Penicillium hirsutum, a total of 933 isolates (432 fungi and 501 bacteria) were isolated from the rhizoshere or rhizoplane of garlics. Among them, Pantoea agglomerans isolate 59-4 (Pa 59-4) was selected for a potential biocontrol agent by in vivo wounded garlic bulb assay, When the spore suspension (10$\^$5/ spores/$m\ell$) of Penicillium hirsutum was co-inoculated with spore or cell suspension of each fungal or bacterial isolate on wounded garlics, the isolate highly suppressed disease development. Soaking garlic bulbs in the suspension of Pa 59-4 significantly reduced garlic decay from p. hirsutum. However, Pa 59-4 did not inhibit the mycelial growth of P. hirsutum in dual-culture with P. hirsutum on Tryptic soy agar. In order to elucidate mode of action of Pa 59-4 nutrient competition between Pa 59-4 and P. hirsutum was investigated using tissue culture plates with cylinder inserts containing defusing membrane reported by Janisiewicz et al. The results showed that Pa 59-4 effectively suppressed spore germination and mycelial growth of blue mold in the low concentration (0.5％) of garlic juice, but did not suppress those of blue mold in the higher concentration (5％) of garlic juice. This result suggests that the mechanism in biocontrol of garlic blue mold by Pa 59-4 may involve in nutrient competition with P. hirsutum on garlic bulbs.