• Nutrition Research and Practice
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• v.10 no.3
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• pp.282-287
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• 2016

BACKGROUND/OBJECTIVES: Jerusalem artichoke has inhibitory activity against ${\alpha}$-glucosidase and decreases fasting serum glucose levels, which may be related to its fructan content. The biological activity of fructan can be influenced by the degree of polymerization. Thus, in this study, the inhibitory effects of original and fermented purple Jerusalem artichoke (PJA) on ${\alpha}$-glucosidase were compared in vitro. Additionally, the anti-diabetes effect of Lactobacillus plantarum-fermented PJA (LJA) was studied in a non-insulin-dependent diabetes mellitus animal model (C57BIKsJ db/db). MATERIALS/METHODS: The water extract of PJA was fermented by L. plantarum, and two strains of Bacillus subtilis to compare their anti-${\alpha}$-glucosidase activities in vitro by ${\alpha}$-glucosidase assays. The anti-diabetes effect of LJA was studied in a non-insulin-dependent diabetes mellitus animal model (C57BIKsJ db/db) for seven weeks. During the experiment, food intake, body weight, and fasting blood glucose were measured every week. At the end of the treatment period, several diabetic parameters and the intestinal ${\alpha}$-glucosidase activity were measured. RESULTS: The LJA showed the highest ${\alpha}$-glucosidase inhibitory activity in vitro. In the in vivo study, it resulted in a significantly lower blood glucose concentration than the control. Serum insulin and HDL cholesterol levels were significantly higher and the concentrations of triglycerides, non-esterified fatty acids, and total cholesterol were significant lower in mice treated with LJA after seven weeks. In addition, the intestinal ${\alpha}$-glucosidase activity was partially inhibited. CONCLUSIONS: These results suggested that LJA regulates blood glucose and has potential use as a dietary supplement.

• Journal of Scientific & Technological Knowledge Infrastructure
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• s.10
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• pp.70-75
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• 2002

우리나라는 생물다양성협약(CBD, Convention on Biological Diversity)의 당사국뿐만 아니라 2001년 5월 국제생물다양성정보기구(GBIF, Global Biodiversity Information Facility)에 투표회원국으로 가입하게됨으로써 국가적 차원의 생물다양성정보 전담기구(National Node)의 지정이 표면화되었고 국내 산재한 관련 DB의 통합, 관련 연구에 집중 및 범세계적 차원으로 대응해야하는 의무가 부여되었다.

• BMB Reports
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• v.39 no.4
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• pp.441-447
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• 2006

Abuse of drugs can elicit compulsive drug seeking behaviors upon repeated administration, and ultimately leads to the phenomenon of addiction. We developed a procedure for the standardization of microarray gene expression data of rat brain in drug addiction and stored them in a single integrated database system, focusing on more effective data processing and interpretation. Another characteristic of the present database is that it has a systematic flexibility for statistical analysis and linking with other databases. Basically, we adopt an intelligent SQL querying system, as the foundation of our DB, in order to set up an interactive module which can automatically read the raw gene expression data in the standardized format. We maximize the usability of this DB, helping users study significant gene expression and identify biological function of the genes through integrated up-to-date gene information such as GO annotation and metabolic pathway. For collecting the latest information of selected gene from the database, we also set up the local BLAST search engine and non-redundant sequence database updated by NCBI server on a daily basis. We find that the present database is a useful query interface and data-mining tool, specifically for finding out the genes related to drug addiction. We apply this system to the identification and characterization of methamphetamine-induced genes' behavior in rat brain.

• Textile Coloration and Finishing
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• v.17 no.2 s.81
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• pp.31-39
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• 2005

This study was conducted to remove the dyes in dye wastewater by the chemical precipitation or biological treatment which are one of the main pollutants in dye wastewater. In order to remove the disperse dyes effectively in aqueous solution by chemical precipitation process, coagulation and flocculation tests were carried out using several coagulants on various reaction conditions. It was found that the Ferrous sulfate was the most effective coagulant for the removal of disperse dye(DB79), and we could get the best result for the removal of disperse dye(DB56) in the aspects of TOC removal efficiency and sludge yield. When the Ferrous sulfate dosage was 800mg/l, the sludge settling velocity was very fast$(SV_{30}=4\%)$, and the color was effectively removed in the disperse dye(DB79) solution. Although the color removal was ineffective when the Alum was used as a coagulant, the sludge yield decreased in comparison with the Ferrous sulfate or the Ferric sulfate being used in the disperse dye(DB56) solution. In order to decolorize disperse dye(DR17) by using biological treatment process, a strain which has potential ability to degrade disperse dyes was isolated from natural system. The optimal culture conditions of temperature and pH were found to be $40^{\circ}C\;and\;8.5\~9$, respectively. When yeast extract was mixed with polypeptone at the mixing ratio of 1:1 as a nitrogen source, decolorization efficiency was highest$(93\%)$ among the nitrogen sources. The strain screened was excellent to adjust to pH, and it seems to have ability to control pH needed to growth. The optimal culture conditions in concentration of $MgSO_{4.}\cdot7H_2O\;and\;KH_2PO_4$ were $0.1\%(w/v)\;and\;0.2\%(w/v)$, respectively. Strains degrading and decolorizing reactive dyes, RB198 and RR141 which were isolated from water system, are named RBK1 and RRK. And the cell growth characteristics of RBK1 and RRK were investigated. The optimal culture conditions of temperature and pH were found to be 30t' and 7.0, respectively. Optimum nitrogen source was peptone, and it was found that decolorization efficiencies by strains RBK1 and RRK, were $85\%\;and\;62\%$, respectively, with introduction of 4,000mg/l of peptone. In the case of RBK1, color removal efficiencies were very high below 400mg/l. Decolorization efficiency was over $90\%$ at 20hours of culture time. The Color degradation ability of RRK was lower than that of RBK1.

• Journal of the Korea Society of Computer and Information
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• v.14 no.6
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• pp.125-134
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• 2009

A biological data acquisition based on web has emerged as a powerful tool for allowing scientists to interactively view entries form different databases, and to navigate from one database to another molecular-biology database links. In this paper, the biological conceptual model is constructed hybrid biological data model to represent interesting entities in the data sources to applying navigation rule property for each biological data source based on four biological data integrating layers to control biological data. When some user's requests for application service are occurred, we can get the data from database and data source via web service. In this paper, we propose a query processing model and execution structure based on integrating data layers that can search information on biological data sources.

• Applied Biological Chemistry
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• v.41 no.6
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• pp.421-425
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• 1998

The amylograph pasting properties of flours $(9{\sim}12%,\;db)$ and starches $(7.5{\sim}9.0%\;db)$ of Korean rice cultivars indicated that the flour concentration to give a fixed peak viscosity was higher by 1.32 times compared with starch, regardless the maturity. Only at 12% flour concentration the peak viscosity and breakdown viscosity were differentiated among maturity groups, which suggested that flour may be better tool than starch in characterizing the maturity groups by pasting properties.

• Nutrition Research and Practice
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• v.9 no.1
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• pp.22-29
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• 2015

BACKGROUND/OBJECTIVES: Recently, anthocyanins have been reported to have various biological activities. Furthermore, anthocyanin-rich purple corn extract (PCE) ameliorated insulin resistance and reduced diabetes-associated mesanginal fibrosis and inflammation, suggesting that it may have benefits for the prevention of diabetes and diabetes complications. In this study, we determined the anthocyanins and non-anthocyanin component of PCE by HPLC-ESI-MS and investigated its anti-diabetic activity and mechanisms using C57BL/KsJ db/db mice. MATERIALS/METHODS: The db/db mice were divided into four groups: diabetic control group (DC), 10 or 50 mg/kg PCE (PCE 10 or PCE 50), or 10 mg/kg pinitol (pinitol 10) and treated with drugs once per day for 8 weeks. During the experiment, body weight and blood glucose levels were measured every week. At the end of treatment, we measured several diabetic parameters. RESULTS: Compared to the DC group, Fasting blood glucose levels were 68% lower in PCE 50 group and 51% lower in the pinitol 10 group. Furthermore, the PCE 50 group showed 2-fold increased C-peptide and adiponectin levels and 20% decreased HbA1c levels, than in the DC group. In pancreatic islets morphology, the PCE- or pinitol-treated mice showed significant prevention of pancreatic ${\beta}$-cell damage and higher insulin content. Microarray analyses results indicating that gene and protein expressions associated with glycolysis and fatty acid metabolism in liver and fat tissues. In addition, purple corn extract increased the phosphorylation of AMP-activated protein kinase (AMPK) and decreased phosphoenolpyruvate carboxykinase (PEPCK), glucose 6-phosphatase (G6pase) genes in liver, and also increased glucose transporter 4 (GLUT4) expressions in skeletal muscle. CONCLUSIONS: Our results suggested that PCE exerted anti-diabetic effects through protection of pancreatic ${\beta}$-cells, increase of insulin secretion and AMPK activation in the liver of C57BL/KsJ db/db mice.

• Journal of Plant Biotechnology
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• v.45 no.4
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• pp.289-298
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• 2018

The Biosafety Division of the National Academy of Agricultural Science has developed a 'Crop Composition DB' that provides analytical data on commercialized crops. It can be used as a reference in the 'Comparative Evaluation by Compositional Analysis' for the safety assessment of genetically modified (GM) crops. This database provides the composition of crops cultivated in Korea, and thus upgrades the data to check the extent of changes in the compositional content depending on the cultivated area, varieties and year. The database is a compilation of data on the antioxidant, nutrient and secondary metabolite compositions of rice and capsicum grown in two or more cultivation areas for a period of more than two years. Data analysis was conducted under the guidelines of the Association of Official Analytical Chemists or methods previously reported on papers. The data was provided as average, minimum and maximum values to assess whether the statistical differences between the GM crops and comparative non-GM crops fall within the biological differences or tolerances of the existing commercial crops. The Crop Composition DB is an open-access source and is easy to access based on the query selected by the user. Moreover, functional ingredients of colored crops, such as potatoes, sweet potatoes and cauliflowers, were provided so that food information can be used and utilized by general consumers. This paper introduces the feature and usage of 'Crop Composition DB', which is a valuable tool for characterizing the composition of conventional crops.

• Journal of the Korean Society of International Agriculture
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• v.23 no.5
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• pp.560-569
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• 2011

In Japan, plant genomics research is mainly leaded by the national research institutes. The various structural studies such as rice genome has allowed researchers to analyze useful traits, and to focus their commercialization. With aims to facilitate structural and functional study in plant genome, NIAS (National Institute of Agrobiological Sciences) constructed Poaceae genome DB and RIKEN (Rikagaku Kenkyusho) built DB for Arabidopsis genome and plant full-length cDNA. NIG (National Institute of Genetics) constructed a national biological resources DB (National Bio Resource Project). This compiling DB provides a variety of genome-related research materials for researchers in the field. Recently, as an effort to resolve global issues of food supply and environmental problems, New Agri-genome Project has been performed aiming to develop an innovative agricultural technologies for the quantity, disease resistance and identifying useful genes related to environmental problems. In addition, in order to improve agricultural productivity in developing countries, JIRCAS assisted technical supports for the plant genome research and developed NERICA rice, which is suitable for African area. Such these approaches are expected to contribute to solving the global issues about food, energy and environment in the world.

• Proceedings of the KOSOMBE Conference
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• v.1994 no.05
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• pp.49-52
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• 1994

We have developed a biological signal measurement, archiving, and communication system (SiMACS). The front end of the system is the intelligent data processing unit (IDPU) which includes ECG, EEG, EMG, blood pressure, respiration, temperature measurement modules, module control and data acquisition unit, real-time display and signal processing unit. IDPUS are connected to central data base unit through LAN(Ethernet). Workstations which receive signals from central DB and provide various signal analysis tools are also connected to the network. The developed PC-based SiMACS is described.