• Journal of Microbiology
• /
• v.41 no.4
• /
• pp.320-326
• /
• 2003

In order to identify Vibrio vulnificus in the Yellow Sea near Gunsan, Korea during the early and late summers, the efficiency of the real-time quantitative TaqMan PCR was compared to the efficiency of the conventional PCR and Biolog identification system^TM. Primers and a probe were designed from the hemolysin/cytolysin gene sequence of V. vulnificus strains. The number of positive detections by real-time quantitative TaqMan PCR, conventional PCR, and the Biolog identification system from seawater were 53 (36.8%), 36 (25%), and 10 strains (6.9%), respectively, among 144 samples collected from Yellow Sea near Gunsan, Korea. Thus, the detection method of the real-time quantitative TaqMan PCR assay was more effective in terms of accuracy than that of the conventional PCR and Biolog system. Therefore, our results showed that the real-time TaqMan probe and the primer set developed in this study can be applied successfully as a rapid screening tool for the detection of V. vulnificus.

• Korean Journal Plant Pathology
• /
• v.13 no.2
• /
• pp.125-128
• /
• 1997

Reactions of Pseudomonas syringae pv. actinidiae to 95 carbon sources in a 96-well microplate (BiOLOG GN MicroPlateTM) were investigated. The bacterium used 9 carbon sources such as D-mannitol, sucrose, etc., but did not use 62 carbon sources such as $\alpha$-cyclodextrin, dextrin, etc. Based on the reactions, a user data base for identification of P. syringae pv. actinidiae was constructed in Biolog program (BiOLOG MicroLogTM 2 system). P. syringae pv. actinidiae isolates collected from kiwifruits could be identified automatically with high similarity using the user data base, which could diagnose rapidly and easily whether the tree was infected with bacterial canker or not.

• Journal of Microbiology
• /
• v.35 no.1
• /
• pp.10-14
• /
• 1997

The database of metabolic fingerprints generated using the GIolog system of lactic acid bacteria isolated from kimchi, described by Lee et al. (8), was used for the identification of 75 Leuconostoc isolates. The test strains were isoalted using a selective isolation medium specific for the genus Leuconostoc and examined for their ability to oxidize carbon sources using the Biolog system. The results show that the 75 test strains were identified to the known Leuconostoce clusters. It is suggested that the Biolog system can be applied for rapid identification of lactic acid bacteria isolated from kimchi.

• Journal of Environmental Health Sciences
• /
• v.34 no.4
• /
• pp.327-332
• /
• 2008

To determine structure and activities of microbial communities in a food wastewater treatment system, biofilm of RABC (rotating activated Bacillus contactor) and samples of aeration tanks were analyzed. Heterotrophic bacterial concentrations were similar between biofilm and stage 1 aeration tank and decreased 2-log at stage 3 aeration tank as dissolved oxygen decreased, however portions of Bacillus groups were increased at stage 3 aeration tank. It was revealed by quantitative and qualitative analysis of metabolic fingerprinting patterns of Biolog GN2 plate that RABC represented much higher activities and a different microbial community structure compared to aeration tanks. Metabolic fingerprinting showed the carbon sources that isolated Bacillus groups could or could not use, were used similarly meaning that not only Bacillus groups but also other microbial groups would contribute to the treatment of wastewater.

• Korean Journal of Food Science and Technology
• /
• v.32 no.6
• /
• pp.1331-1335
• /
• 2000

The WL6 strain isolated from Kimchi could not be made scientific name because it was identified as three species, i.e., Leuconostoc mesenternides ssp cremoris, Leu. mesenteroides ssp. dextranicum or Lactobacillus bifermentans when it was tested by API kit or Biolog system methods. The unidentifiable WL6 strain was finally reclassified as Lactobacillus bifermentans by genetic identification using two PCR-based specific sequence primer sets which were originated from homologous pepN and 16S rRNA genes.

• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 2002.11a
• /
• pp.43.2-44
• /
• 2002

• Korean Journal of Microbiology
• /
• v.40 no.3
• /
• pp.248-253
• /
• 2004

To scrutinize the physiological diversity by BIOLOG microplate, the carbon source utilization patterns of 168 strains of oligotrophic bacteria and 132 strains of psychrotrophic bacteria isolated from Lake Baikal during 2000 and 2002 were investigated. Eighty-six percent (56 strains) of oxidase test positive group (GN-NENT group) and 89 % (92 strains) of oxidase test negative group (GN-ENT group) among oligotrophic bacteria, and 82% (85 strains) of oxidase test negative group among psychrotrophic bacteria were able to utilize $\alpha$-D-glucose as a sole-carbon-source, and 93% (26 strains) of oxidase test positive group among psychrotrophic bacteria were able to utilize bromosuccinic acid as a sole-carbon-source. However, most strains except few oligotrophic bacteria with oxidase test negative group were not able to utilize $\alpha$-D-lactose as a sole-carbon-source. Most dominant genus among 300 strains was Pseudomonas (49 strains). Other dominant genera belonged to Salmonella, Serratia, Buttiauxella, Pantoea, Yersinia, Brevundimonas, Hydrogenophaga, Photorhabdus, Sphingomonas, and Xenorhabdus. Our results by BIOLOG identification system were able to provide basic data to determine community-level carbon source utilization patterns and to accomplish the efficient and reliable identification for microbial community structure in Lake Baikal.

• Journal of Microbiology and Biotechnology
• /
• v.12 no.1
• /
• pp.77-83
• /
• 2002

A bacterial consortium capable of utilizing a variety of polycyclic aromatic hydrocarbons has been isolated from a former manufactured gas plant site. The consortium consisted of four members including Arthrobacter sp., Burkholderia sp., Ochrobacterium sp., and Alcaligenes sp., which were identified and characterized by the patterns of fatty acid methyl esters (FAME analysis) and carbon source utilization (BIOLOG system). With the individual members, the biodegradation characteristics of aromatic hydrocarbons depending on different growth substrates were determined. FAME analyses demonstrated that microbial fatty acid profiles changed to significant extents in response to different carbon sources, and hence, such shift profiles may be informative to characterize the biodegradation potential of a bacterium or microbial community.

• Research in Plant Disease
• /
• v.12 no.2
• /
• pp.115-118
• /
• 2006

In February of 2000-2001, the gray mold disease occurred on nephrolepis (Nephrolepis sp.) grown in a flower nursery farm in Suwen, Korea. Typical symptoms were water-soaked brown or blackish lesions on terminal leaf blades. Severely infected leaves were entirely blighted with grayish fungal mycelia formed on the surface. Conidia of the fungus in mass were hyaline or gray, 1-celled, mostly ellipsoid or ovoid and $13.5{\sim}16.9{\times}6.8{\sim}9.2{\mu}m$ in size. Conidiophores were formed on PDA with $8.7{\sim}11.1{\mu}m$ in width. The sclerotia were readily formed within 2 or 3 days on PDA. In addition, the Biolog database gave the causal fungus a high similarity to Botrytis cinerea (78%) with a match probability of 100%. Pathogenicity of the causal organism was proved according to Koch's postulate. The causal organism was identified as Eotrytis cinerea based on its mycological characteristics and utilization of carbon sources with Biolog system as supporting data. This is the first report of gray mold of nephrolepis caused by Botrytis cinerea in Korea.

• Journal of Life Science
• /
• v.14 no.6 s.67
• /
• pp.891-894
• /
• 2004

The effect of pipe materials on biofilm communities were investigated by CLPP (community level physiology profile) using Biolog GN plates. Heterotrophic bacterial concentrations were $10^4\;-\;10^6\;CFU/cm^2$ and there was no differences between galvanized iron and carbon steel. Average optical density of Biolog plate was similar between two pipe materials. However, CLPP was different according to the type of pipe materials and exposed times to tap water, and CLPP was independent of bacterial concentration. This represents the differences of bacterial communities with pipes and water contact times.