• Toxicological Research
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• 제34권1호
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• pp.1-6
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• 2018

The purpose of this study was to detect cell death in the liver of mice treated with thioacetamide (TAA) using fluorescence bioimaging and compare this outcome with that using conventional histopathological examination. At 6 weeks of age, 24 mice were randomly divided into three groups: group 1 (G1), control group; group 2 (G2), fluorescence probe control group; group 3 (G3), TAA-treated group. G3 mice were treated with TAA. Twenty-two hours after TAA treatment, G2 and G3 mice were treated with Annexin-Vivo 750. Fluorescence in vivo bioimaging was performed by fluorescence molecular tomography at two hours after Annexin-Vivo 750 treatment, and fluorescence ex vivo bioimaging of the liver was performed. Liver damage was validated by histopathological examination. In vivo bioimaging showed that the fluorescence intensity was increased in the right upper part of G3 mice compared with that in G2 mice, whereas G1 mice showed no signal. Additionally ex vivo bioimaging showed that the fluorescence intensity was significantly increased in the livers of G3 mice compared with those in G1 or G2 mice (p < 0.05). Histopathological examination of the liver showed no cell death in G1 and G2 mice. However, in G3 mice, there was destruction of hepatocytes and increased cell death. Terminal deoxynucleotidyl transferase dUTP nick end labeling staining confirmed many cell death features in the liver of G3 mice, whereas no pathological findings were observed in the liver of G1 and G2 mice. Taken together, fluorescence bioimaging in this study showed the detection of cell death and made it possible to quantify the level of cell death in male mice. The outcome was correlated with conventional biomedical examination. As it was difficult to differentiate histological location by fluorescent bioimaging, it is necessary to develop specific fluorescent dyes for monitoring hepatic disease progression and to exploit new bioimaging techniques without dye-labeling.

• Interdisciplinary Bio Central
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• 제4권2호
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• pp.5.1-5.9
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• 2012

Small peptide tags such as c-myc, HA, or FLAG tag have facilitated efficient Western-blotting of proteins of interest especially when specific antibodies for the proteins are not available. However, the conventional Western-blotting requires the multi-steps process taking at least several hours up to two days. With examples of various applications, here we show a convenient and time-saving method for protein detection which employs a fluorescent chemical BDED and its binding peptide RC-tag. And we propose "Estern staining", as a standard term for protein detection method using fluorescent chemicals and their binding small peptide tags. Eastern staining may substitutes for the time-consuming "immuno-staining" in many versatile applications.

• Bulletin of the Korean Chemical Society
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• 제31권12호
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• pp.3623-3631
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• 2010

We prepared several novel molecular transporters built on myo- and scyllo-inositol scaffolds with variations in the number of guanidine residues, linker chain lengths and patterns. Some of these transporters were found to localize in mitochondria, and the mitochondrial affinity seems to be substantially related to the scaffold stereochemistry.

• 세라미스트
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• 제21권3호
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• pp.270-282
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• 2018

The increasing importance of biomedical imaging technology has led to the development of a variety of luminescent materials, including molecular fluorophores, fluorescent proteins, and quantum dots. Owing to their inherent disadvantages, such as insufficient chemical stability and limited biocompatability, their utilization has been limited with imaging only under highly optimized and controlled conditions. Recently, a new class of luminescent nanoparticles, upconversion nanoparticles (UCNPs), have been emerging as a practically useful nanoprobe for various bioimaging applications. The detailed synthesis, functionalization, properties and in-vitro / in-vivo applications of the UCNPs are introduced and discussed in this Review.

• Journal of Plant Biotechnology
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• 제43권1호
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• pp.21-29
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• 2016

Remote sensing는 각종 센서를 이용하여 지표면, 물, 요소 기술에 대해 비접촉, 비파괴적인 방법으로 필요한 정보를 얻어내는 기술이다. 이들 기술은 센서 등의 요소 기술 뿐만 아니라, 센서를 탑재하는 플랫폼과 정보 통신 기술(ICT) 등을 복합적으로 이용한다. 특히 농업 분야에서는 ICT를 중개로 기상이나 토양 등의 환경 정보와 작물 정보를 측정하여 수치화하고 클라우드 컴퓨팅에 의해 생산 단계뿐만 아니라 유통 및 소비 단계까지 관리하는 스마트 농업에 크게 기여한다. 식물을 측정하기 위해서는 비파괴 비접촉 bioimaging (remote imaging)을 포함한 식물기능 remote sensing 기술개발이 필요하다. 또한 식물 과학 분야에서도 유전자 세포 수준에서 개체 수준까지를 대상으로 한 bioimaging 연구가 활발히 진행되고 있다. 최근 들어 표현형 연구를 통해 환경과 유전자형의 관계를 구명하는 phenomics 연구가 활발히 진행되고 있다. 따라서 본 논문에서는 식물기능 원격탐사의 기술동향, 식물진단 및 식물환경응답해석과 식물 phenomics 연구현황에 대해 고찰하였다.

• 한국고분자학회지:고분자과학과기술
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• 제23권5호
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• pp.509-512
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• 2012

• 한국광학회:학술대회논문집
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• 한국광학회 2006년도 하계학술발표회 논문집
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• pp.35-35
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• 2006

• Investigative Magnetic Resonance Imaging
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• 제23권1호
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• pp.17-25
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• 2019

We discuss recent advances in Gd-based $T_1$-weighted MR contrast agents for the mapping of cellular pH. The pH plays a critical role in various biological processes. During the past two decades, several MR contrast agents of strategic importance for pH-mapping have been developed. Some of these agents shed light on the pH fluctuation in the tumor microenvironment. A pH-responsive self-assembled contrast agent facilitates the visualization of tumor size as small as $3mm^3$. Optimization of various parameters is crucial for the development of pH-responsive contrast agents. In due course, the new contrast agents may provide significant insight into pH fluctuations in the human body.

• 한국광학회:학술대회논문집
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• 한국광학회 2008년도 동계학술발표회 논문집
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• pp.271-272
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• 2008

• 한국고분자학회지:고분자과학과기술
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• 제19권2호
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• pp.130-137
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• 2008