• Applied Chemistry for Engineering
• /
• v.34 no.2
• /
• pp.121-125
• /
• 2023

Casein is a milk protein and one of the most important nutrients in milk. The composition is over 80% in cow's milk and about 20~45% in human's milk. Casein is highly biocompatible and biodegradable, so it has been studied for various biomedical materials applications as well as drug delivery systems. It is widely known that casein can be prepared as nanoparticles in the presence of the Ca²⁺ metal ion. Because casein is amphiphilic, hydrophobic drugs could be loaded inside to form a protein-based drug delivery system. In this study, we studied the optimum conditions for casein nanoparticle formation using natural metal ions present in the body, such as calcium, magnesium, zinc, and iron. It was confirmed that nanoparticles have a uniform size of around 150 nm and negative zeta potential values. In addition, it was demonstrated that casein nanoparticles have a cell viability of more than 80% and efficient intracellular uptake properties using confocal microscopy. From the results, it was also shown that the casein nanoparticles prepared using various metal ions have the potential to be biocompatible drug delivery carriers.

• Advances in nano research
• /
• v.14 no.6
• /
• pp.507-519
• /
• 2023

Postsurgical infections are caused by implant-related pathogenic microorganisms that lead to graft rejection. Hence, an intrinsically antibacterial material is required to produce a biocompatible biomaterial with osteogenic properties that could address this major issue. Hence, this current research aims to make strontium-doped hydroxyapatite nanorods (SrHANRs) via an ethylene diamine tetraacetic acid (EDTA)-enable microwave mediated method using Anodontia alba seashells for biomedical applications. This investigation also perceives that EDTA acts as a soft template to accomplish Sr-doping and mesoporous structures in pure hydroxyapatite nanorods (HANRs). The X-ray diffraction (XRD) and transmission electron microscopy (TEM) analysis reveals the crystalline and mesoporous structures, and Brunauer-Emmett-Teller (BET) indicates the surface area of all the samples, including pure HANRs and doped HANRs. In addition, the biocidal ability was tested using various implant-related infectious bacteria pathogens, and it was discovered that Sr-doped HANRs have excellent biocidal properties. Furthermore, toxicity evaluation using zebrafish reports the non-toxic nature of the produced HANRs. Incorporating Sr²⁺ ions into the HAp lattice would enhance biocompatibility, biocidal activity, and osteoconductive properties. As a result, the biocompatible HANRs materials synthesized with Sr-dopants may be effective in bone regeneration and antibacterial in-built implant applications.

• Proceedings of the KSME Conference
• /
• 2007.05b
• /
• pp.3187-3190
• /
• 2007

Elastin-Like polypeptide (ELP) composed of elastin-based repeating units is an artificial biomaterial which is biocompatible and non-immunogenic. ELP shows a characteristic inverse phase transition between hydrophobic and hydrophilic phase by external stimuli such as salt, pH and temperature. In this study, ELP coated PS (polystyrene) beads are packed in tubing and the thermo -responsive flow characteristics of the packed bed are investigated. Preliminary test results show that the control of the fluid flow can be achieved by using the temperature driven phase transition effect of the ELP coated beads in a microchannel.

• Proceedings of the KOSOMBE Conference
• /
• v.1991 no.05
• /
• pp.74-78
• /
• 1991

Conventional compression bone fracture plates sometimes cause osteoporosis under the plate due to their high rigidity which in turn transfer physiological load mostly through the plates and screws. In order to prevent the osteopenia we have designed a system which have a viscoelastic washer between plate and screw head. The washer is made of a biocompatible ploymer (untra high molecular weight polyethylene, UHMWPE). Three-dimensional finite element meshes of the human femur with the conventional and new concept bone plate ere generated and the comparative stress analyses are performed with static half-stance loading condition. The results of analyses showed that could reduce the stress shielding effect compared with the conventional plate.

• Bulletin of the Korean Chemical Society
• /
• v.33 no.10
• /
• pp.3378-3382
• /
• 2012

A superhydrophobic surface with a water contact angle > $150^{\circ}$ has attracted great interest from both fundamental and practical aspects. In this study, we demonstrated that hydrophobicity of a silica nanotube (SNT) array can be easily controlled by the SNT aspect ratio. In addition, the adhesive and anti-adhesive properties were controlled without modifying the hydrophobic surface. Various silica structures on a polydimethylsiloxane substrate were prepared using the desired alumina template. Bundle-arrayed and bowl-arrayed silica surfaces exhibited extraordinary superhydrophobicity due to the large frontal surface area and hierarchical micro/nanostructure. As the strategy used in this study is biocompatible and a wide range of hydrophobicities are capable of being controlled by the SNT aspect ratio, a hydrophobic surface composed of an SNT array could be an attractive candidate for bioapplications, such as cell and protein chips.

• Advances in nano research
• /
• v.2 no.3
• /
• pp.135-145
• /
• 2014

Nanomaterials synthesized by natural bioresources such as microorganisms, animals and plants in nature can also be synthesized in laboratories even on large scale. This is considered as an attractive prospect for eco-friendly or so-called green synthesis. Development of eco-friendly synthesis of biocompatible nanoparticles and their potential biomedical applications introduces the concept of nanobiotechnology. The lower cost and lesser side effects as compare to chemical methods of synthesis are the main advantages of biosynthesis. This review article demonstrates the role of various biological resources e.g. bacteria, fungi, actinomycetes, plant leaves, fruits and honey as well as animal tissues for the synthesis of nanoparticles mainly gold and silver with an overview of their potential applications.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.5
• /
• pp.612-619
• /
• 2015

We have synthesized gold nanoparticles (GNPs) using chicken feathers (poultry waste) and Bacillus subtilis RSE163. Disulfide reductase and keratinase produced by Bacillus subtilis during the degradation of chicken feather has been used to reduce Au³⁺ from HAuCl₄ precursor to produce gold nanoparticles. The synthesized biogenic GNPs were characterized by UV-visible spectroscopy, transmission electron microscopy (TEM), and zeta potential measurements. Fourier transform infrared (FTIR) spectroscopy indicated the presence of protein capping on synthesized GNPs, imparting multifunctionality to the GNP surface. Furthermore, the nontoxic nature of biogenic GNPs was insured by interaction with Escherichia coli (ATCC11103), where TEM images and enhancement of growth rate of E. coli in log phase signified their nontoxic nature. The results indicate that the synthesis of biocompatible GNPs using poultry waste may find potential applications in drug delivery and sensing.

• Ceramist
• /
• v.21 no.3
• /
• pp.283-292
• /
• 2018

Here, we introduce various type of inorganic nanostructure synthesized with functional nanoparticles and silica. From two decades ago, functional inorganic nanoparticles have been synthesized and highlighted, now we moved to next level of wet-chemical synthesis. By integrating functional nanoparticles with silica, we were able to synthesize multi-functional nanostructure, which expand the applications of nanoparticles to catalyst, drug carrier, sensors. In this context, silica has been spotlighted due to its versatility. Silica has highly biocompatible, relatively transparent and stable under harsh conditions. Thus it can be used as good supporter to synthesize complex multi-functional nanostructure when mixed with other functional nanoparticles. A various shape of complex nanostructures have been synthesized including core-shell type, yolk-shell type and janus type etc. In this paper, we have described the purposes of synthesizing silica noncomplex and various case studies for biomedical applications and self-assembly.

• Journal of the Korean Ceramic Society
• /
• v.56 no.6
• /
• pp.521-525
• /
• 2019

A study was conducted to investigate the possibility of a composite material containing a composite resin as a matrix and hydroxyapatite (HAp) powder as a substitute material for tooth repair. As the content of HAp increased, hardness value (111.9 HV at 9%) increased and flexural strength (73.3 MPa at 9%) decreased. Observation of the microstructure after immersion in a simulated body fluid (SBF) solution confirmed a dense structure due to mutual coagulation and curing. It was thought that fine HAp recrystals were formed with the lapse of time, and they were entangled to form a condensation structure and had a dense structure. In addition, since the activity was shown by the ion migration on the surface of a tooth, it was highly likely that a biocompatible bond occurred during tooth contact. Therefore, it could be used as a substitute material for tooth repair.

• 한국생물공학회:학술대회논문집
• /
• 2003.10a
• /
• pp.644-647
• /
• 2003

Materials currently used as an injectable bulking agent in the dermatologic and urologic fields revealed several drawbacks such as particles migration, inflammatory reaction, allergic reaction, rapid volume shrinkage, and necessity of a donor site. In this study, we have developed injectable biomaterial comprising poly (DL- lactide-co-glycolide)(PLGA) and hyaluronic acid gel to overcome these problems. PLGA is a biocompatible synthetic material and hyaluronic acid is a common substance found in living organisms. We examined the feasibility of injection through needle and tested biocompatibility in animal model. After transplantation, injected sites and distant organs were examined histologically to verify a new tissue formation, inflammation, and particles migrations. Injected volume was maintained approximately 80 percent for 2 months. Results demonstrated that the developed material was injectable through various gauges of needles and induced a new bulking tissue formation without serious inflammatory reaction.