• 분석과학
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• 제33권5호
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• pp.224-231
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• 2020

With the improvement in the standard of living and extension of life expectancy, the incidence of prostate diseases has increased yearly, thus becoming a serious disease affecting the health of men. The extract mixture of Cornus officinalis and Stauntonia hexaphylla leaves is a developed functional food formula to improve prostate health. This study developed a simultaneous analytical method of bioactive compounds for quantifying the mixture of Cornus officinalis and S. hexaphylla leaves using high-pressure liquid chromatography-ultraviolet (HPLC-UV). HPLC analytical condition was performed on a Hector C₁₈ column with a mobile phase of 0.1 % formic acid in water (A) and 0.1 % formic acid in acetonitrile (B) under the following gradient conditions: 0-50 min, 12 %-40 % (B) at a flow rate of 1.0 mL/min. Meanwhile, this method was validated properly and successfully used to quantify the bioactive components of morroniside and hederacoside D in 20 sample batches and assess the quality of different ages and seasons of S. hexaphylla leaves. The result showed that the content of morroniside in the extract mixture of Cornus officinalis and S. hexaphylla leaves ranged from 1.38-1.62 mg/g, and the hederacoside D ranged from 28.42-32.02 mg/g, suggesting that this novel analytical method will be suitable for the quality control of the extract mixture to improve benign prostatic hyperplasia.

• 생명과학회지
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• 제28권2호
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• pp.229-239
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• 2018

안전하고 건강지향적인 식품에 관한 소비자들의 관심이 증가함에 따라, 학계와 산업계에서는 생리활성 기능을 가진 소재를 이용한 식품을 개발하려는 시도가 많이 이루어지고 있는 추세이다. 특히 합성물질보다는 생리활성작용을 하는 천연물질의 이용이 증가되고 있는데 이러한 이유는 합성화학물질과 달리 천연물질은 그 이용에 대한 제한이 없고 소비자들의 신뢰도가 높기 때문이다. 폐계육의 가슴살과 명태살을 이용한 게맛살을 제조할 때 동충하초분말, 누에고치분말 및 CLA의 혼합 첨가가 게맛살의 이화학적 품질특성에 미치는 효과를 구명하기 위하여 폐계육 회수단백질과 명태살에 누에고치분말(10 g)을 첨가한 T1, 누에고치분말(5 g)과 동충하초분말(5 g)을 첨가한 T2, 동충하초분말(5 g)과 공액리놀랜산(5 g)(CLA)를 첨가한 T3를 제조하여 실험을 수행한 결과를 요약하면 다음과 같다. pH와 전단가는 저장초기에 비교해 저장 2주 이후에 모든 처리구에서 유의적으로 증가하는 경향을 나타내었다. 전단가는 6주에서 동충하초분말과 CLA를 혼합 첨가한 구(T3)에서 유의적으로 높게 나타났다. 육색의 경우 적색도($a^{\ast}$)와 백색도(W)는 저장기간 동안 모든 구간에서 높게 나타난 반면에 황색도($b^{\ast}$)는 저장기간 동안 감소하였다. T2의 적색도($a^{\ast}$), 황색도($b^{\ast}$)가 다른 처리구보다 상당히 높게 나타내었다. 생리활성물질의 첨가는 게맛살의 겔 특성과 조직감에 영향을 미치지 않았다. 지방산패도(TBARS)와 총균수(TPC)는 T1이 다른 처리구보다 상당히 높게 나온 반면에 단백질변패도(VBN)의 경우 다른 처리구보다 T2가 높게 나왔다. T1과 T3는 저장기간 동안 총 아미노산함량이 감소하였다. 결과적으로 동충하초분말 0.5%와 CLA 0.5% 혼합 첨가한 T3 처리구가 종합적인 품질면에서 폐계육 함유 게맛살의 기능성을 가장 높일 수 있을 것으로 사료된다.

• 한국세라믹학회지
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• 제52권5호
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• pp.374-379
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• 2015

Mullite-matrix and $Al_2O_3$-matrix composites were fabricated with 30 wt% hydroxyapatite (HA) and tricalcium phosphate (TCP), respectively, as additives to give bioactivity. A diphasic gel process was employed to lower the densification temperature of the mullite matrix to $1320^{\circ}C$. A polymer complexation process was used to synthesize a TCP powder that was fully densified at $1250^{\circ}C$, for application to the matrix. For the HA/mullite composite, HA decomposed during sintering by reactions with the matrix components of $Al_2O_3$ and $SiO_2$, resulting in a mixture of $Al_2O_3$, TCP, and other minor phases with a low densification of less than 88% of the theoretical density (TD). In contrast, the TCP/$Al_2O_3$ composite was highly densified by sintering at $1350^{\circ}C$ to 96%TD with no reaction between the components. Different from the TCP monolith, the TCP/$Al_2O_3$ composite also showed a fine microstructure and intergranular fracture, both of which characteristics are advantageous for strength and fracture toughness.

• Mass Spectrometry Letters
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• 제14권2호
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• pp.25-35
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• 2023

Plants are a traditional source of many chemicals used as biochemical, flavors, food, color, and pharmaceuticals in various countries, especially India. Most herbal medicines and their derivatives are often made from crude extracts containing a complex mixture of various phytochemical chemical components (secondary metabolites of the plants). This study aimed to identify bioactive compounds from the different parts of the plant from the ethanolic extract of Gymnema sylvestre, Senna auriculata, and Cissus quadrangularis (leaves, flower, stem) by gas chromatography-mass spectroscopy (GC-MS). The gas chromatography - mass spectrometry analysis revealed the presence of various compounds like 3,4-dimethylcyclohexanol, hexanoic acid, D-mannose, and N-decanoic acid. Hence, the Gymnema sylvestre, Senna auriculata, and Cissus quadrangularis may have chemopreventive, anti-cancer, anti-microbial activity, antioxidant, anti-diabetic activity, anti-inflammatory, and antifungal due to the presence of secondary metabolites in the ethanolic extract. These phytochemicals are supported for traditional use in a variety of diseases.

• Journal of Dairy Science and Biotechnology
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• 제34권3호
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• pp.165-172
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• 2016

Kefir, originating from Caucasus, is an acidic, alcoholic fermented milk product with little acidic taste and a creamy consistency. It is recognized in having beneficial effects infor the prevention and treatment of cancer. For example, Kefir has possesses a chemopreventative effect on carcinogenesis. There has recently been a strong focus on fermented milk foods containing a mixture of several functional organic substances and various probiotic microorganisms. Hence, the purpose of this review paper was to evaluate the scientific evidence for the effects of kefir on cancer prevention and treatment. Some of we analyzed and summarized data-relating to the effects of kefir on cancer. The cacers that kefir has an effect on are as follows: colon cancer, breast cancer, leukemia, sarcoma, skin cancer, gastric cancer. This review suggests that (1) kefir could be associated with cancer prevention, (2) kefir has beneficial effects in cancer treatment, and (3) kefir has various bioactive components including peptides, polysaccharides and sphingolipids, which contribute tofor itsthese anti-cancer properties. Furthermore, furthermore, studies were performed in order to obtain as to get the scientific evidence of kefir's anticancer activity: (1) improved protective effectiveness in vivo (human subjects or animal model), (2) isolation and identification of various bioactive components, and (3) mechanisms associated with beneficial effects.

• Journal of Microbiology and Biotechnology
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• 제26권12호
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• pp.2138-2140
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• 2016

The goal of this study was to identify a source of natural plant compounds with inhibitory activity against pepper mild mottle virus (PMMoV). We showed, using a half-leaf assay, that murrayafoline-A (1) and isomahanine (2) isolated from the aerial parts of Glycosmis stenocarpa have inhibitory activity against PMMoV through curative, inactivation, and protection effects. Using a leaf-disk assay, we confirmed that 2 inhibited virus replication in Nicotiana benthamiana. Using electron microscopy, we found that a mixture of the virus with 2 resulted in damage to the rod-shaped virus.

• 한국식품영양과학회지
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• 제30권6호
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• pp.1204-1209
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• 2001

본 연구는 한국에 널리 분포되어 있는 대표적인 침엽수중에 혈액지질에 영향을 미치는 성분이 있는지를 검색하는 것을 목적으로 시도되었다. 1차적으로 미국측백과 뚝향나무의 유기 용매와 수용성 추출물을 흰쥐에게 경구 투여하여 체중 증가, 장기 무게, 혈액지질성분 및 혈당의 변화를 관찰하였다. 추출물의 섭취는 체중증가와 간, 신장, 비장과 같은 장기무게에 유의적인 차이가 없어 일반적인 성장에는 영향을 주지 않는 것으로 보인다. 그러나 체내 저장지방은 뚝향나무의 유기용매층 섭취군에서 유의적으로 낮았다. 그리고 혈청내 총 지방량은 고지방 대조군이 저지방 대조군에 비해 유의적으로 높았으며, 뚝향나무의 유기용매층이 유의적으로 낮아 뚝 향나무의 유기용매층이 혈액 지질저하 효과가 있는 것으로 선정되었다. 2차적으로 뚝향나무의 유기용매층을 3분획하여 횐쥐를 대상으로 혈액지질성분과 혈당, 빈혈지표등을 관찰하였다. 결과를 보면 JCDM2가 체내 지방축적량을 유의적으로 낮추었으며, 혈청내 총 콜레스테롤과 중성지방량이 유의적이지는 않으나 낮은 경향을 보였다. 그리고 JCDM2의 50%이상을 차지하고 있는 물질로 communic acid가 순수 분리되었다. 본 연구는 아직 시도되지 않았던 수목의 혈액내 지질 저하효과를 관찰하는 기초연구로 실험 결과, 뚝향나무의 유기용매층에서 효과가 있었으며 그 주요 물질은 communic acid로 관찰되었다.

• Journal of Ginseng Research
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• 제42권4호
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• pp.412-418
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• 2018

Background: Ginsenoside Rg3(S) and compound K (C-K) are pharmacologically active components of ginseng that promote human health and improve quality of life. The aim of this study was to produce Rg3(S) and C-K from ginseng extract using recombinant Lactococcus lactis. Methods: L. lactis subsp. cremoris NZ9000 (L. lactis NZ9000), which harbors ${\beta}$-glucosidase genes (BglPm and BglBX10) from Paenibacillus mucilaginosus and Flavobacterium johnsoniae, respectively, was reacted with ginseng extract (protopanaxadiol-type ginsenoside mixture). Results: Crude enzyme activity of BglBX10 values comprised 0.001 unit/mL and 0.003 unit/mL in uninduced and induced preparations, respectively. When whole cells of L. lactis harboring pNZBglBX10 were treated with ginseng extract, after permeabilization of cells by xylene, Rb1 and Rd were converted into Rg3(S) with a conversion yield of 61%. C-K was also produced by sequential reactions of the permeabilized cells harboring each pNZBgl and pNZBglBX10, resulting in a 70% maximum conversion yield. Conclusion: This study demonstrates that the lactic acid bacteria having specific ${\beta}$-glucosidase activity can be used to enhance the health benefits of Panax ginseng in either fermented foods or bioconversion processes.

• Journal of Dairy Science and Biotechnology
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• 제41권3호
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• pp.113-125
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• 2023

In this study, we explored the synergistic effects of whey protein concentrate (WPC) and soybean protein components after fermentation with lactic acid bacteria isolated from kimchi, and identified several peptides with desirable physiological functions, proteolysis, and immune effects. Antioxidant activity was determined using 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid, 1,1-diphenyl-2-picrylhydrazyl, ferric-reducing antioxidant power, and hydroxyl radical scavenging assays, followed by cross-validation of the four antioxidant activities. These assays revealed that samples with a 8:2 and 9:1 whey to soy ratio possessed higher antioxidant activity than the control samples. Antibacterial potency testing revealed high antibacterial activity in the 9:1 and 8:2 samples. Cytotoxicity testing of samples using 3-(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide revealed that only the 10:0, 1:9, and 0:10 samples had <80% viable cells, indicating no significant cytotoxicity. Nitric oxide (NO) assays revealed that NO expression was reduced in 8:2, 5:5, and 0:10 protein ratio fermentations, indicating low inflammatory reaction stimulatory potential. Cytokine expression was confirmed using an enzyme-linked immunosorbent assay kit. The 8:2 sample had the lowest inflammatory cytokine (interleukin [IL]-1α, IL-6, and tumor necrosis factor-α) levels compared with the lipopolysaccharide-treated group. Amino acid profiling of the 8:2 sample identified 17 amino acids. These results suggest that inoculating and fermenting Lactobacillus plantarum DK203 and Lactobacillus paracasei DK209 with an 8:2 mixture of WPC and soybean protein releases bioactive peptides with excellent anti-inflammatory and antioxidant properties, making them suitable for functional food development.

• 생명과학회지
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• 제24권4호
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• pp.386-392
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• 2014

본 연구에서는 야생산사씨의 항산화 활성을 알아보고자 70% methanol, 70% ethanol, chloroform:methanol (CM, 2:1, v / v), n-butanol, ethyl acetate (EA) 용매로 추출하여, 시료 농도 0.2 mg/ml, 0.5 mg/ml, 0.8 mg/ml에서 측정 비교 하였다. DPPH, reducing power, FRAP 및 ABTS radical 소거활성은 70% methanol, 70% ethanol, EA, CM, n-butanol 추출물 순으로 높게 측정되어 모두 같은 경향을 나타내었다. 특히, DPPH에서 70% methanol 추출물은 18.22%~65.42%, 70% ethanol 추출물은 15.97%~60.25%의 우수한 radical 소거 활성을 보였다. NO radical 소거 활성은 n-butanol, EA, CM, 70% methanol, 70% ethanol 추출물 순으로 나타났으며, $OH^-$ radical 소거 활성은 n-butanol, 70% methanol, 70% ethanol, CM, EA 추출물 순으로 측정되었다. 모든 용매 추출물은 대조군으로 사용한 표준물질에 비해서는 유의적으로 항산화 활성이 낮은 것으로 나타났다. 산사씨 용매 추출물 중 70% methanol 및 70% ethanol 추출물의 항산화 활성이 대부분 높은 경향이었다. 따라서 산사씨는 우수한 천연 항산화제로 활용이 가능할 것으로 사료된다.