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http://dx.doi.org/10.4014/jmb.1607.07034

Inhibitory Components from Glycosmis stenocarpa on Pepper Mild Mottle Virus  

Kim, Jang Hoon (Department of Horticultural and Crop Environment, National Institute of Horticultural and Herbal Science, RDA)
Yoon, Ju-Yeon (Department of Horticultural and Crop Environment, National Institute of Horticultural and Herbal Science, RDA)
Kwon, Sun Jung (Department of Horticultural and Crop Environment, National Institute of Horticultural and Herbal Science, RDA)
Cho, In Sook (Department of Horticultural and Crop Environment, National Institute of Horticultural and Herbal Science, RDA)
Nguyen, Manh Cuong (Department of Bioactive Products, Vietnam Academy of Science and Technology)
Choi, Seung-Kook (Department of Horticultural and Crop Environment, National Institute of Horticultural and Herbal Science, RDA)
Kim, Young Ho (College of Pharmacy, Chungnam National University)
Choi, Gug Seoun (Department of Horticultural and Crop Environment, National Institute of Horticultural and Herbal Science, RDA)
Publication Information
Journal of Microbiology and Biotechnology / v.26, no.12, 2016 , pp. 2138-2140 More about this Journal
Abstract
The goal of this study was to identify a source of natural plant compounds with inhibitory activity against pepper mild mottle virus (PMMoV). We showed, using a half-leaf assay, that murrayafoline-A (1) and isomahanine (2) isolated from the aerial parts of Glycosmis stenocarpa have inhibitory activity against PMMoV through curative, inactivation, and protection effects. Using a leaf-disk assay, we confirmed that 2 inhibited virus replication in Nicotiana benthamiana. Using electron microscopy, we found that a mixture of the virus with 2 resulted in damage to the rod-shaped virus.
Keywords
Pepper mild mottle virus; Glycosmis stenocarpa; isomahanine; electron miscroscopy; antiviral agent;
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