• Journal of Plant Biotechnology
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• v.34 no.1
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• pp.1-6
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• 2007

Effect of BA concentrations and culture methods on in vitro plant multiplication from shoot-tip cultures of Wasabia japonica was studied. Shoot-tips with leaf primordia and apical meristem were cultured on MS basal medium for all the experiments. Liquid medium for 2 weeks followed by semi-solid medium for 4 weeks containing 1.0 mg/L BA was the best to number of shoots (22.8) and shoot length (3.5 cm). Shoots proliferated could be divided into ca. 5 to 11 of cultures for the multiplication of plantlets. Divided plantlets showed root formation (90%) well onto MS basal medium without growth regulators like IBA and NAA. After rooting, all the plantlets transferred into the pots containing composed soil (bio-media Co., peatmoss $8{\sim}10%$, coir dust $66{\sim}70%$, zeolite $13{\sim}17%$, vermiculite $3{\sim}7%$, perlite $2{\sim}4%$) and grown well into whole plants with multiple shoots.

• Journal of Korean Society on Water Environment
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• v.27 no.4
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• pp.467-474
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• 2011

In an effort to investigate water pollution characteristics of Juam lake, water samples were collected from three sites (Sites A, B, and C) of Oenam stream which is a typical tributary of rural watershed in the lake and analyzed for N concentration and the corresponding isotope ratio (${\delta}^{15}N$) of ${NO_3}^-$. Concentrations of ${NO_3}^-$ were not dramatically different among the sites; $0.8{\pm}0.2mgNL^{-1}$ (range: $0.0{\sim}4.3mgNL^{-1}$) for Site A, $1.1{\pm}0.2mgNL^{-1}$ ($0.0{\sim}4.3mgNL^{-1}$) for Site B, and $1.1{\pm}0.1mgNL^{-1}$ ($0.1{\sim}2.6mgNL^{-1}$) for Site C. Meanwhile, ${\delta}^{15}N$ tended to decrease with river flow; it was highest for Site A ($45.5{\pm}5.3$‰) followed by Site B ($19.7{\pm}2.0$‰) and Site C ($8.7{\pm}1.5$‰). Such high ${\delta}^{15}N$ values of ${NO_3}^-$ in Site A suggested that ${NO_3}^-$ derived from livestock feedlot (specifically livestock excrete of which ${\delta}^{15}N$ is higher than 10‰) is the predominant pollution sources despite mountainous area occupied the most of land-use in the watershed. Using the two-sources isotope mixing model, it was estimated that the contribution of cropping activities (i.e. fertilization) became greater in down-stream area (Sites B and C) due to the higher agricultural land-use than the up-stream area (Site A). Particularly, during the active cropping season, the low contribution of organic pollution sources indicated that domestic sewage was not the predominant pollution source. Therefore, it was suggested that agricultural sources such as livestock farming and cropping rather than mountainous and residential are the dominant sources of water pollution in the study area. These results could be effectively utilized in elucidating water pollution sources in rural areas and selecting water management practices.

• Journal of Ginseng Research
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• v.32 no.1
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• pp.26-32
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• 2008

A total of 29 Botrytis were isolated from ginseng gray mold at 8 locations in Korea from June to July, 2004 and 2005. The causative agent of them was identified as Botrytis cinerea based on morphological characteristics of conidia and RPB2 gene sequence analysis. In inoculation experiments with sclerotia, one isolate was pathogenic to ginseng plant whereas two isolates were non-pathogenic. Scanning electron microscopy revealed that the sclerotia of pathogenic isolate has rough surface and that of non-pathogenic isolate very smooth surface. Optimum temperature and culture conditions for sclerotia production were $5{\sim}20^{\circ}C$ and darkness, respectively. The number of sclerotia was increased tenfold on media added with 0.5% old stem fragment of ginseng.

• Journal of Hydrogen and New Energy
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• v.13 no.1
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• pp.34-41
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• 2002

CdS and $TiO_2$ nanoparticles were made by the precipitation method and sol-gel method, respectively, and they were mixed mechanically and then treated with the hydrothermal processing. CdS-$TiO_2$ composite particulate films were thus prepared by casting CdS-$TiO_2$ mixed sol onto $SnO_2$ conducting glass and a subsequent heat-treatment at $400^{\circ}C$. Again, the physico-chemical and photoelectrochemical properties of these films were controlled by the surface treatment with $TiCl_4$ aqueous solution. The photocurrents and the hydrogen production rates measured under the present experimental conditions varied in the range of $3.5{\sim}4.5mA/cm^2$ and $0.3{\sim}1.8cc/cm^2$-hr, respectively, and showed the maximum values at the $CdS/[CdS+TiO_2]$ mole ratio of 0.2. Also, the surface treatment with $TiCl_4$ aqueous solution caused a considerable improvement in the photocatalytic activity, Probably as a result of close contacts between the primary particles by the etching effect of $TiCl_4$ It was found that the photoelectrochemical performance of these particulate films could be effectively enhanced by this approach.

• Carbon letters
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• v.3 no.1
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• pp.1-5
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• 2002

The regional bio-wastes available in abundance in India were converted into porous carbon by heat treatment at different temperatures from $650-950^{\circ}C$. The wood retain shapes after pyrolysis though shrinkage occured both in axial and radial directions. The shrinkage in radial direction was found to be more than in axial direction in all woods. The density of woods and chars from these at a given temperature has been found to follow linear relationship. Chars were steam activated at temperature $700-800^{\circ}C$ for different times between 45-240 min. Both the temperature and time of activation with steam has a profound effect on surface area. Chars from softwoods like bagasse and castor oil plant were activated at lower temperature, i.e. $700-750^{\circ}C$ whereas hard wood chars have to be activated at higher temperature around $800^{\circ}C$. The morphology of wood as well as of chars has been studied by SEM. The comparison of the two showed that the nature of porosity in chars depends on precursor morphology, nature and physical state of wood and presence of inorganic compounds in the wood. Hard wood results in cross inter connected pores while softwood leads to fibriller structure. The present studies show that activated carbon with reasonably good surface area (${\sim}1000m^2/gm$) can be prepared from soft wood bio-wastes like bagasse and castor oil plant, while surface area ${\sim}1370m^2/gm$ was achieved from hard wood bio waste of pine wood.

• Journal of Microbiology and Biotechnology
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• v.19 no.8
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• pp.760-764
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• 2009

In this study, we investigated the antifungal activity of three different forms of silver nanoparticles against the unidentified ambrosia fungus Raffaelea sp., which has been responsible for the mortality of a large number of oak trees in Korea. Growth of fungi in the presence of silver nanoparticles was significantly inhibited in a dose-dependent manner. We also assessed the effectiveness of combining the different forms of nanoparticles. Microscopic observation revealed that silver nanoparticles caused detrimental effects not only on fungal hyphae but also on conidial germination.

• Analytical Science and Technology
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• v.28 no.1
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• pp.17-25
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• 2015

A rapid, sensitive and specific method was developed and validated using electrospray ionization (ESI) tandem mass spectrometry (LC-MS/MS) for determination of misoprostol acid in human plasma. Misoprostol $acid-d_5$ was used as in internal standard (IS). The analyte and IS were extracted by simple one step solid phase extraction (SPE). Linearity in plasma was obtained over the concentration range 10~3000 pg/mL and lower limit of quantification (LLOQ) was identifiable and reproducible at 10 pg/mL. The intra- and inter-day precision values were below 9% and the accuracy was ranged from 93.81% to 102.02% at all four quality control samples. The method was has been successfully applied for routine assay to support pharmacokinetic study of misoprostol acid in human plasma after an oral administration of 0.4 mg misoprostol.

• Textile Coloration and Finishing
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• v.21 no.4
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• pp.33-38
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• 2009

Poly(vinyl acetate) films containing benzophenone were photocrosslinked by continuous UV irradiation.UV irradiation of PVAc film containing 5% benzophenone induced bulk crosslinking of the polymer indicated by 84.1% of gel fraction after ethyl acetate extraction. The crosslinking was attributed to the recombination of tertiary polymer radicals generated upon UV irradiation, which was enhanced by the hydrogen abstraction of benzophenone. Also the UV irradiation resulted in scission of ester linkage and photooxidation of PVAc surface, which was verified by ATR and zeta potential analysis, implying that the PVAc surface became more polar and hydrophilic. The zeta potential proportionally increased from +4.5mV to -26.8 mV with increasing UV irradiation. Also the surface energy of the PVAc film increased with higher UV irradiation upto 56.5 $mJ/m^2$ by the enhanced Lewis acid/base component with larger contribution of Lewis acid parameter. Accordingly the crosslinked PVAc showed higher thermal stability with increasing UV energy.

• Journal of Embryo Transfer
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• v.33 no.4
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• pp.337-342
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• 2018

Cryopreservation is mainly used for preservation of boar sperm. However, this method stresses the sperm by reactive oxygen species (ROS), and the conception rate and the litter size are not more efficient than the liquid preservation of spermatozoa. Therefore, we use chitosan which is a natural product derived antioxidant compound. We used GnHA (chitosan+hyaluronic acid) and GnHG (chitosan hydrogel) as chitosan complexes to cryopreserve boar sperm for improve sperm metabolism and function. Sperm parameter (sperm motility, progressive motility, path velocity, straight-line velocity, curvilinear velocity) is measured by computer-assisted sperm analysis (CASA) using frozen sperm with GnHA or GnHG (0, 0.25, 0.5, 1 mg/mL), respectively. Also, lipid peroxidation analysis using malondialdehyde (MDA) is performed to confirm the antioxidative effect of chitosan in frozen spermatozoa. CASA analysis showed GnHA and GnHG are effective against cryopreserved boar sperm. And antioxidant effect is measured by lipid peroxidation analysis. GnHA and GnHG, which is chitosan complex are effective for boar sperm cryopreservation by antioxidant effect.

• Journal of Bio-Environment Control
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• v.16 no.2
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• pp.115-120
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• 2007

An experiment was conducted to measure rooting and growth of cuttings of rose as affected by cutting length, number of leaflets and cutting position. Test plants were two cultivars of Rosa hybrida 'Red Sandra'(standard) and 'Little Marble'(miniature). Cutting length was 4, 7 or 10cm for 'Red Sandra' and 3, 5 or 7 cm for 'Little Marble'. The number of leaflets left on the five-leaflet leaf was 0, 2, 4 or 5. The cutting position on the stem was between $1{\sim}2,\;3{\sim}4,\;5{\sim}6\;or\;7{\sim}8$ nodes from the shoot tip, which have five leaflet leaves. The most efficient cutting length was 7cm in both cultivars. Treatment with all leaflets left and cutting position at $1{\sim}2$ nodes resulted in good rooting ratio for both cultivars.