• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.1010-1010
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• 2005

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.1012-1012
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• 2005

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.934-935
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• 2005

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.942-942
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• 2005

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.965-965
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• 2005

• Bulletin of the Korean Chemical Society
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• v.35 no.8
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• pp.2581-2584
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• 2014

• Bulletin of the Korean Chemical Society
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• v.27 no.2
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• pp.325-328
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• 2006

• Bulletin of the Korean Chemical Society
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• v.32 no.1
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• pp.347-349
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• 2011

• Bulletin of the Korean Chemical Society
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• v.30 no.8
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• pp.1870-1872
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• 2009

• Molecules and Cells
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• v.25 no.2
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• pp.312-316
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• 2008

Flavanone $3{\beta}$-hydroxylases (F3H) are key enzymes in the synthesis of flavonol and anthocyanin. In this study, three F3H cDNAs from Oryza sativa (OsF3H-1 ~3) were cloned by RT-PCR and expressed in E. coli as gluthatione S-transferase (GST) fusion proteins. The purified recombinant OsF3Hs used flavanone, naringenin and eriodictyol as substrates. The reaction products with naringen and eriodictyol were determined by nuclear magnetic resonance spectroscopy to be dihydrokaempferol and taxifolin, respectively. OsF3H-1 had the highest enzymatic activity whereas the overall expression of OsF3H-2 was highest in all tissues except seeds. Flavanone $3{\beta}$-hydroxylase could be a useful target for flavonoid metabolic engineering in rice.