• Asian-Australasian Journal of Animal Sciences
• /
• v.16 no.10
• /
• pp.1550-1554
• /
• 2003

One hundred of lactic cultures were evaluated for their ability to inhibit hazardous microbes, such as Salmonella enteritidis, Salmonella typhimurium, Escherichia coli, Listeria monocytogenes, and Bacillus cereus by agar well diffusion method. None of them showed inhibitory halo against S. enteritidis, while 27 strains showed inhibitory activity against S. typhimurium, 6 against E. coli, 9 against ampicillin resistant E. coli, 31 against L. monocytogens, 10 against B. cereus. pH of the culture does not explain for the inhibitory activity except against B. cereus. A neutralized culture from corn silage showed highest inhibitory activity against S. typhimurium, and the size of inhibitory halo was same as 10 ug/mL of ampicillin. The culture was identified to be Lactobacillus buchneri on the basis of biochemical characteristics and utilization of substrates. Using the culture as probiotics could be expected to reduce antibiotics for animal feeding.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.16 no.12
• /
• pp.8665-8672
• /
• 2015

In this study, to investigate the feasibility of malodorous substance and organic matter removal by digest sludge in sewage treatment plants, ammonia, methyl mercaptan(MMC), and hydrogen sulfide($H_2S$) in a reactor submerged with BIO-CLOD(BIO-CLOD) and a reactor not submerged with BIO-CLOD(Non BIO-CLOD) were measured at 24 hours, 48 hours, and 72 hours after the submergence of BIO-CLOD. Whereas the reactor in which BIO-CLOD was submerged showed an ammonia removal rate of 48% and high $H_2S$ and MMC removal rates exceeding 98% in 24 hours, the reactor in which BIO-CLOD was not submerged showed an ammonia removal rate of 45%, an $H_2S$ removal rate of 71%, and an MMC removal rate of 84% in 24 hours indicating the possibility of removal of malodor using BIO-CLOD. A nitrification was shown in which ammonia concentrations decreased over time while nitrate nitrogen concentrations increased and sulfur based malodor components were oxidation decomposed indicating that BIO-CLOD had effects to increase sulfate concentrations in the solution and that sulfate concentration increases and atmospheric $H_2S$ removal rates were correlated with each other. With regard to decreases in organic matter in reactor effluents, BIO-CLOD did not affect in a short period of time and when reactors were operated with HRT 12 hours and HRT 24 hours, HRT 12 hours was considered desirable in terms of economy.

• Journal of Microbiology and Biotechnology
• /
• v.19 no.2
• /
• pp.147-154
• /
• 2009

The carboxylesterase-encoding gene(bioHs) of a newly isolated strain, Serratia sp. SES-01, was cloned from the genomic DNA library by detecting formation of transparent halo around the colony on LB-tributyrin agar plates. The amino acid sequence of BioHs was highly similar to the members of the BioH enzyme family involved in the biotin biosynthetic pathway; it showed the highest similarity(91%) with that of Serratia proteamaculans. To compare BioHs with other BioH enzymes, the relatively well-known bioHe gene of E. coli was cloned with PCR. After we achieved high-level expression of soluble BioHs and BioHe through the exploration of different culture conditions, the purified BioHs and BioHe enzymes were characterized in terms of specificity, activity, and stability. BioHe was generally more robust to a change in temperature and pH and an addition of organic solvents than BioHs. The two enzymes exhibited a strong preference for carboxylesterase rather than for thioesterase and were optimal at relatively low temperatures($20-40^{\circ}C$) and alkaline pHs(7.5-9.0). The results in this study strongly suggested that both the BioHs and BioHe enzymes would be potential candidates for use as a carboxylesterase in many industrial applications.

• Reproductive and Developmental Biology
• /
• v.29 no.2
• /
• pp.93-99
• /
• 2005

Human embryonic stem cells (hESCs) derived from the inner cell mass of blastocysts have the ability to renew themselves and to differentiate into cell types of all lineage. The present study was carried out to investigate whether the Wnt signaling pathway is related to maintaining self-renewal of hESCs. Glycogen Synthase Kinase 3 (GSK-3) inhibitor, BIO ((2'Z,3'E)-6-Bromoindirubin-3'-oxime) was treated to Miz-hES1 line for activation of Wnt signaling pathway. BIO-nontreated hESCs (control) and BID-treated hESCs were cultured for 5 days in the modified feeder-free system. During the culture of hESCs, differences were observed in the colony morphology between 2 groups. Controls were spread outwards whereas BIO-nontreated hESCs were clumped in the center and the differentiated cells were spreading outwards in the edges. The results of stem cell specific marker staining indicated that control were differentiated in large part whereas BIO-treated hESCs maintain self-renewal in the center of the colony. The results of lineage marker staining suggested that outer cells of the hESC colony were differentiated to the neuronal progenitor cells in both control and BIO-treated hESC. These results indicate that Wnt signaling is related to self-renewal in hESCs. In addition, control group showed higher composition of apoptotic cells $(23.76\%)$ than the BID-treated group $(5.59\%)$. These results indicate that BIO is effective on antapoptosis of hESCs.

• Asian-Australasian Journal of Animal Sciences
• /
• v.16 no.12
• /
• pp.1799-1803
• /
• 2003

The possibility of lycopene affecting egg yolk pigmentation was studied with lycopene diets containing 0, 4, 8, and $12{\mu}g/g$ meal, respectively. The addition of lycopene above $4{\mu}g/g$ meal significantly improved yolk color after four days of supplementation. The transfer of lycopene into egg yolk was confirmed by thin layer chromatography, and high-performance liquid chromatography-mass spectrometry (HPLC-MS). The deposition rate of lycopene into egg yolk was approximately 2%, which was quantitatively determined using a HPLC with a UV detector. The result indicates that lycopene is a good candidate for egg yolk pigmentation and for making functional eggs.

• Bulletin of the Korean Chemical Society
• /
• v.29 no.1
• /
• pp.81-84
• /
• 2008

In the present study, we report 1H and 13C NMR data of 19 methoxyflavonol derivatives with different substitution patterns on A- and B-ring. In addition, the influence of the methoxy substituents in A- and B-ring on the 1H and 13C NMR chemical shifts is discussed: the 1H and 13C chemical shifts of and the number of methoxyl groups provided information allowing elimination of many structural isomers from consideration and in certain instances greatly simplified structural elucidation.

• Molecules and Cells
• /
• v.25 no.2
• /
• pp.312-316
• /
• 2008

Flavanone $3{\beta}$-hydroxylases (F3H) are key enzymes in the synthesis of flavonol and anthocyanin. In this study, three F3H cDNAs from Oryza sativa (OsF3H-1 ~3) were cloned by RT-PCR and expressed in E. coli as gluthatione S-transferase (GST) fusion proteins. The purified recombinant OsF3Hs used flavanone, naringenin and eriodictyol as substrates. The reaction products with naringen and eriodictyol were determined by nuclear magnetic resonance spectroscopy to be dihydrokaempferol and taxifolin, respectively. OsF3H-1 had the highest enzymatic activity whereas the overall expression of OsF3H-2 was highest in all tissues except seeds. Flavanone $3{\beta}$-hydroxylase could be a useful target for flavonoid metabolic engineering in rice.

• 한국초전도학회:학술대회논문집
• /
• 2007.07a
• /
• pp.59-59
• /
• 2007

• 한국초전도학회:학술대회논문집
• /
• 2007.07a
• /
• pp.58-58
• /
• 2007

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2013.05a
• /
• pp.1511-1512
• /
• 2013