• 제목/요약/키워드: Bio-tech

검색결과 277건 처리시간 0.03초

In Vitro Antagonistic Activity Evaluation of Lactic Acid Bacteria (LAB) Combined with Cellulase Enzyme Against Campylobacter jejuni Growth in Co-Culture

  • Dubois-Dauphin, Robin;Sabrina, Vandeplas;Isabelle, Didderen;Christopher, Marcq;Andre, Thewis;Philippe, Thonart
    • Journal of Microbiology and Biotechnology
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    • 제21권1호
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    • pp.62-70
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    • 2011
  • The antibacterial effects of nine lactic acid bacteria (LAB) against Campylobacter jejuni were investigated by using agar gel diffusion and co-culture assays. Some differences were recorded between the inhibition effects measured with these two methods. Only two LAB, Lb. pentosus CWBI B78 and E. faecium THT, exhibited a clear anti- Campylobacter activity in co-culture assay with dehydrated poultry excreta mixed with ground straw (DPE/GS) as the only growth substrate source. It was observed that the supplementation of such medium with a cellulase A complex (Beldem S.A.) enhanced the antimicrobial effect of both LAB strains. The co-culture medium acidification and the C. jejuni were positively correlated with the cellulase A concentration. The antibacterial effect was characterized by the lactic acid production from the homofermentative E. faecium THT and the lactic and acetic acids production from the heterofermentative Lb. pentosus CWBI B78. The antagonistic properties of LAB strains and enzyme combination could be used in strategies aiming at the reduction of Campylobacter prevalence in the poultry production chain and consequently the risk of human infection.

TP53I11 suppresses epithelial-mesenchymal transition and metastasis of breast cancer cells

  • Xiao, Tongqian;Xu, Zhongjuan;Zhang, Hai;Geng, Junsa;Qiao, Yong;Liang, Yu;Yu, Yanzhen;Dong, Qun;Suo, Guangli
    • BMB Reports
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    • 제52권6호
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    • pp.379-384
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    • 2019
  • Epithelial-mesenchymal transition (EMT) is widely-considered to be a modulating factor of anoikis and cancer metastasis. We found that, in MDA-MB-231 cells, TP53I11 (tumor protein P53 inducible protein 11) suppressed EMT and migration in vitro, and inhibited metastasis in vivo. Our findings showed that hypoxic treatment upregulated the expression of $HIF1{\alpha}$, but reduced TP53I11 protein levels and TP53I11 overexpression reduced $HIF1{\alpha}$ expression under normal culture and hypoxicconditions, and in xenografts of MDA-MB-231 cells. Considering $HIF1{\alpha}$ is a master regulator of the hypoxic response and that hypoxia is a crucial trigger of cancer metastasis, our study suggests that TP53I11 may suppress EMT and metastasis by reducing $HIF1{\alpha}$ protein levels in breast cancer cells.

LOP와 Petri Dish의 수분 보호능에 관한 비교 연구 (Comparative Studied on Moisture-Protection Ability of LOP and Petri Dish)

  • 이진성;서유미;강창열;정용대;이상수;오정균
    • 한국산학기술학회:학술대회논문집
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    • 한국산학기술학회 2008년도 추계학술발표논문집
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    • pp.465-468
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    • 2008
  • 현재 가장 범용적으로 사용되는 미생물 배양 용기인 Petri Dish는 배지 제조 후 냉장과 실온 보관 시수분 증발에 대한 보호 효과가 적어 세 달 이상 보관하기 어려운 단점이 있어 즉시 사용 배지(pre-plated media) 개발에 장애가 되고 있다. 본 연구는 미생물 배지의 장기 저장이 가능하도록 고안된 LOP plate의 수분 보호능에 관한 것으로, LB 배지를 표준 배지로 해서 4, 25, 32 및 $37^{\circ}C$ 네 개의 온도영역과 진공 포장 및 비 포장 조건에서 Petri Dish와 비교한 결과, 각 온도 조건별로 LOP의 탁월한 수분 보호능을 확인 할 수 있었다. 따라서 향후 LOP를 이용한 장기 저장과 즉시 사용이 가능한 pre-plated media와 관련된 제품 개발이 가능할 것으로 기대된다.

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과일폐기물을 이용한 DEFC용 바이오에탄올 제조 및 특성에 관한 연구 (A Study on Characteristic of the Bio-ethanol Produced on Fruit Wastes for Direct Ethanol Fuel Cell (DEFC))

  • 이남진;김현수;차인수;최정식
    • 한국수소및신에너지학회논문집
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    • 제22권2호
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    • pp.257-264
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    • 2011
  • This study discribes performance of DEFC (Direct Ethanol Fuel Cell) utilized bio-ethanol based on fruit wastes. To produce the bio-ethanol, fruit wastes were treated at temperature $120^{\circ}C$ and 90minutes in acid pre-treatment. After pre-treatment was done, alcohol fermentation process was running. Initial alcohol concentration was 5%. Using the multi coloumn distillation system, more than 95% ethanol was distilled and each component of bio-ethanol was analyzed. In DEFC performance test, it was revealed that cell performance was much higher than that of ethanol. Comparing ethanol with mixed fuel (bio-ethanol (10%) + ethanol (90%)), the performance of ethanol was higher than that of mixed fuel. Even though the bio-ethanol from the fruit wastes is corresponded with transport ethanol standards, it thought that organic matter in bio-ethanol could be negative effect on fuel cell.

팥 에탄올 추출물의 Papain 유도 관절염 마우스에서의 항 골관절염 효과 (Effect of Vigna angularis Ethanol Extract on Papain-induced Arthritis in Mice)

  • 정시화;김승형;김현규;윤보라;이희웅;임주환;노문철;김동희
    • 동의생리병리학회지
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    • 제26권5호
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    • pp.665-671
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    • 2012
  • The purpose of this study is to examine the effect of Vigna angularis ethanol extract (VA-E) on papain induced arthritis in C57BL/10 mice. The C57BL/10 mice were injected with papain and oral administration of Vigna angularis ethanol extract (VA-E) at different doses (100 and 200 mg/kg) once a day for 42 days and compared with untreated mice. Histological examination and the measurement of inflammatory cytokine release were performed. The results showed that comparing with untreated mice, the treatment with Vigna angularis ethanol extract (VA-E) decreased the pathological changes of knee joint tissue. The level of inflammatory cytokine secreted from the tissues, such as IL-$1{\beta}$ (interleukin-$1{\beta}$), IL-6 (interleukin-6), TNF-${\alpha}$ (Tumor Necrosis Factor-${\alpha}$), were decreased. These results were confirmed by the quantification of cartilage volume using micro CT scanning. Consequently, Vigna angularis ethanol extract (VA-E) has a therapeutic potential in inflammatory joint diseases such as osteoarthritis.

Reduction of Variable Illumination Effect on Pixel Gray-levels of Machine Vision

  • Suh S. R.;Huang J. K.;Kim Y. T.;Yoo S. N.;Choi Y. S.;Sung J. H.
    • Agricultural and Biosystems Engineering
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    • 제5권1호
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    • pp.5-9
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    • 2004
  • This study was carried out to develop methods of reducing the effect of solar illumination on pixel gray-levels of machine vision for agricultural field use. Two kinds of monochrome CCD cameras with manual and auto-iris lenses were used to take pictures within a range of 15 to 120 klux of solar illumination. A camera having more precise automatic control functions gave much better result. Four kinds of indices using pixel gray-level of the $99\%$ white DRS (diffuse reflectance standard) as a reference were tried to compensate pixel gray-levels of an image for variable illumination. Coefficients of variation of the indices within a range of illumination were used as a criterion for comparison. The study concluded that an index of (A+B)/A, where A is gray-level of the $99\%$ DRS and B is gray-level of the tested material, gave the best consistency in the range of solar illumination.

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Bio-tech 피혁 가공제의 적용에 따른 작업 환경의 개선, 작업공정의 표준화 및 물성증진에 관한 연구 (Studies Betterment of Work Environment, Standardization of Work Process, and Improvement of the Properties of Leather Based on the Application of Bio-tech Leather Processing Agent)

  • Han, Sang Duck;Lee, JongChul;Lee, Jong Seok;Yang, DaeYong;Cho, Young Jin;Kim, Hyung Jin
    • 산업경영시스템학회지
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    • 제18권36호
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    • pp.23-30
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    • 1995
  • 생활용품을 포함한 제조산업에 관계되는 환경오염 문제가 심각한 국내문제로 제시되어 왔으며, 제혁산업은 심각한 환경문제를 야기 시키고 있다. 본 연구는 Bio-tech 피혁가공제로 Lupin LE-10 을 개발하여 탈모공정에 적용하고자 하며, 화학적 탈모방법과 비교를 통하여 Lupin LE-10 탈모 방법의 여러 가지 장점의 검토를 하고자 한다. 따라서, 본 연구는 개발한 Bio-tech 피혁가공제를 효해공정과 수적공정에 적용하여 Lupin LE-10의 유용성과 제혁산업으로부터 야기하는 환경문제의 개선, 가죽의 수율과 물성증진에 기여하고자 한다.

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