• Bulletin of the Korean Chemical Society
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• 제26권8호
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• pp.1168-1169
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• 2005

• 융복합기술연구소 논문집
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• 제6권1호
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• pp.7-11
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• 2016

An experiment was performed to show the bio-signal characteristics of the Stuckyi when was heated. To measure the bio-signal of the plant, this study used the electrode with copper(+) and constantan(-). The electrode was directly inserted into the Stuckyi stems. And used T-type thermocouple to measure the inside temperature of the Stuckyi. During the experiments, room temperature also measured with T-type thermocouple. Heating was made with hair dryer periodically that 3 times turned on for heating 5 min and off for non-heating 25 min. Under the situation, the responses of the Stuckyi including voltage potential and inside temperature were measured using data logger(HP-34970A) according to the distances(15cm, 30cm, 45cm) between hair dryer and the plant. Through the experiments, We knew that the inside temperature has similar level and behavior with the room temperature at normal state. And during heating period, the inside temperature and the potential were also simultaneously increased sharply. From the experiments, we revealed that the measuring system of bio-signal of the plant being proposed in this study can be useful to show the Characteristics of the plants.

• Bulletin of the Korean Chemical Society
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• 제27권9호
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• pp.1329-1334
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• 2006

The electrochemical reduction of coumarin, 7-acetoxy-4-methyl coumarin (AMC), and 7-acetoxy-4-bromomethyl coumarin (ABMC), in 0.1 M tetraethyl ammonium perchlorate/acetonitrile solution was carried out by direct current, differential pulse polarography, cyclic voltammetry, and controlled potential coulometry. The electrochemical reduction of ABMC was proceeded through three steps of electron transfer coupled with the chemical reactions. The color of solution was changed to yellow when the carbonyl group was reduced during 2nd step (-1.8 volts) and independented with cleavage of bromo group. Highest fluorescence intensity showed when the electrochemical reduction of AMC was controlled at near the potential (-2.3 volts vs. Ag/AgCl).

• Asian Pacific Journal of Cancer Prevention
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• 제13권2호
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• pp.699-704
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• 2012

Natural products have been the target for cancer therapy for several years but there is still a dearth of information on potent compounds that may protect normal cells and selectively destroy cancerous cells. The present study was aimed to evaluate the cytotoxic potential of n-butanolic leaf extract of $Annona$ $muricata$ L. on WRL-68 (normal human hepatic cells), MDA-MB-435S (human breast carcinoma cells) and HaCaT (human immortalized keratinocyte cells) lines by XTT assay. Prior to cytotoxicity testing, the extract was subjected to phytochemical screening for detecting the presence of compounds with therapeutic potential. Their relative antioxidant properties were evaluated using the reducing power and $DPPH^*$radical scavenging assay. Since most of the observed chemo-preventive potential invariably correlated with the amount of total phenolics present in the extract, their levels were quantified and identified by HPLC analysis. Correlation studies indicated a strong and significant (P<0.05) positive correlation of phenolic compounds with free radical scavenging potential. The results revealed that the extract was moderately cytotoxic to normal cells with a mean IC50 value of 52.4 ${\mu}g$ when compared with those obtained for cancerous cells (IC50 values of 29.2 ${\mu}g$ for MDA-MB-435S and 30.1 ${\mu}g$ for HaCaT respectively). The study confirms the presence of therapeutically active antineoplastic compounds in the n-butanolic leaf extract of $Annona$ $muricata$. Isolation of the active metabolites from the extract is in prospect.

• Molecules and Cells
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• 제38권7호
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• pp.657-662
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• 2015

Rapid and efficient engulfment of apoptotic cells is an essential property of phagocytes for removal of the large number of apoptotic cells generated in multicellular organisms. To achieve this, phagocytes need to be able to continuously uptake apoptotic cells. It was recently reported that uncoupling protein 2 (Ucp2) promotes engulfment of apoptotic cells by increasing the phagocytic capacity, thereby allowing cells to continuously ingest apoptotic cells. However, the functions of Ucp2, beyond its possible role in dissipating the mitochondrial membrane potential, that contribute to elevation of the phagocytic capacity have not been determined. Here, we report that the anion transfer or nucleotide binding activity of Ucp2, as well as its dissipation of the mitochondrial membrane potential, is necessary for Ucp2-mediated engulfment of apoptotic cells. To study these properties, we generated Ucp2 mutations that affected three different functions of Ucp2, namely, dissipation of the mitochondrial membrane potential, transfer of anions, and binding of purine nucleotides. Mutations of Ucp2 that affected the proton leak did not enhance the engulfment of apoptotic cells. Although anion transfer and nucleotide binding mutations did not affect the mitochondrial membrane potential, they exerted a dominant-negative effect on Ucp2-mediated engulfment. Furthermore, none of our Ucp2 mutations increased the phagocytic capacity. We conclude that dissipation of the proton gradient by Ucp2 is not the only determinant of the phagocytic capacity and that anion transfer or nucleotide binding by Ucp2 is also essential for Ucp2-mediated engulfment of apoptotic cells.

• 대한방사선기술학회지:방사선기술과학
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• 제39권1호
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• pp.35-42
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• 2016

CdZnTe(CZT)는 상온에서 동작 가능한 II-VI족 기반의 화합반도체로 CT (Computed Tomography)나 맘모그라피 (mammography)용 검출기로 적용하면, 환자의 피폭선량을 저감할 수 있는 획기적인 소자재료이다. 픽셀(pixel)과 픽셀 피치(pixel pitch)에 따라 X선 변환효율과 신호 교차 (cross-talk)에 영향을 주어 영상 품질이 결정된다. 가중 퍼텐셜 (weighting potential)은 전극의 위치와 형태에 의해서 결정지어지는 가상 퍼텐셜로 Poisson's 방정식의 해를 통해서 구할 수 있다. 본 연구에서는 컴퓨터 기반의 모의실험을 통해 가상 퍼텐셜을 계산하고, 전하유도효율(CIE; charge induction efficiency)과 신호교차를 고려하여 CT용 센서에 적합한 픽셀을 결정하고자 하였다. 모의실험에서 1 mm의 픽셀피치와 2 mm 두께의 CZT를 가정하여, 다양한 픽셀과 픽셀피치를 설정 후 가중 퍼텐셜을 계산하였다. 픽셀의 크기가 $750{\mu}m$이고 픽셀간의 간격이 $250{\mu}m$일 때 최대 전하유도 효율과 최소 신호교차를 나타내었다.

• 전기화학회지
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• 제11권3호
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• pp.165-169
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• 2008

CODH(Carbon Monoxide Dehydrogenase)에 의한 이산화탄소 환원에 있어서 작업전극을 유리탄소전극을 사용한 경우와 금전극을 사용한 경우를 비교하여 그 영향을 관찰하였다. 금전극을 사용한 경우에는 수소발생과 섞이기 때문에 전기분해의 전위를 잘 선택해야 효율적인 이산화탄소의 환원 반응을 관찰할 수 있는데 반하여, 유리탄소전극은 금전극보다 수소 환원에 대한 과전압이 크기 때문에 -650 mV vs. NHE 까지도 중성수용액에서 수소발생 없이, 효율적인 이산화탄소의 환원을 관찰할 수 있었다. CODH를 이용한 이산화탄소의 환원에는 가해주는 전기분해 전위가 큰 영향을 미침을 알 수 있었는데, $-570{\sim}600\;mV$ vs. NHE 근처가 가장 효율적임을 알 수 있었고 이보다 더 음의 전위를 걸어주었을 때는 효소활성의 감소 및 수소발생이 복합적으로 영향을 미쳐 일산화탄소 생성의 전류효율이 급격히 감소함을 알 수 있었다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제37권5호
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• pp.415-420
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• 2011

Purpose: Calcium phosphate cement (CPC) is one of many useful materials for restoring tooth defects, periodontium and maxillofacial area. Chitosan is a biodegradable material that has been shown to promote the growth and differentiation of osteoblasts in culture. This study examined the interaction between odontoblasts and bio-calcium phosphate cement reinforced with chitosan. Materials and Methods: $5{\times}10^3$ odontoblastic cells were seeded into each well. Various concentrations of bio-calcium phosphate cement reinforced with chitosan (10, 20, 50, 100, 200, 500 ${\mu}g$/ml, 1, 2, 4 mg/ml) were diluted and added to the wells. The well was incubated for 24 h, 48 h and 72 h. After incubation, the number of cells was assessed to determine the cell viability. A cytokinesis-block micronucleus assay and chromosomal aberration test were carried out to estimate the extent of chromosomal abnormalities. Microscopic photographs and RT-PCR were performed to examine the adhesion potential of bio-calcium phosphate cement reinforced with chitosan. Results: Bio-CPC-reinforced chitosan did not show significant cytotoxicity. The number of damaged chromosomes in the cells treated with Bio-CPC-reinforced chitosan was similar to that in the control cells. There was no significant increase in the number of chromosomal aberrations in the Bio-CPC reinforced chitosan exposed cells. Microscopic photographs and RT-PCR confirmed the adhesive potential of bio-CPC reinforced chitosan to odontoblasts. Conclusion: Bio-CPC-reinforced chitosan did not affect the odontoblastic cell viability, and had no significant cytotoxic effect. Bio-CPC-reinforced chitosan showed adhesive potential to odontoblasts. These results are expected form the basis of future studies on the effectiveness of dental restorative materials in Bio-CPC reinforced with chitosan.

• 한국산업융합학회 논문집
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• 제4권1호
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• pp.11-19
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• 2001

This study was carried out to investigate the feasibility of the used frying oil as a bin-oil which was one of the alternative fuel for diesel engine. From tests of engine performance, it was shown that the bio-oil and blends and the sufficient potential as alternative fuels of diesel engine except NOx and Smoke emission.

• Interdisciplinary Bio Central
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• 제3권1호
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• pp.5.1-5.5
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• 2011

Introduction: Many signal transduction pathways mediate cell's behavior by regulating expression level of involved genes. Abnormal behavior indicates loss of regulatory potential of pathways, and this can be attributed to loss of expression regulation of downstream genes. Therefore, function of pathways should be assessed by activity of a pathway itself and relative activity between a pathway and downstream genes, simultaneously. Results and Discussion: In this study, we suggested a new method to assess pathway's function by introducing concept of 'responsiveness'. The responsiveness was defined as a relative activity between a pathway itself and its downstream genes. The expression level of a downstream gene as a function of an upstream pathway activation characterizes disease status. In this aspect, by using the responsiveness we predicted potential progress in cancer development. We applied our method to predict primary and metastatic status of melanoma cancer. The result shows that the responsiveness-based approach achieves better performance than using gene or pathway information alone. The mean of ROC scores in the responsiveness-based approach was 0.90 for GSE7553 data set, increased more than 40% compared to a gene-based method. Moreover, identifying the abnormal regulatory patterns between pathway and its downstream genes provided more biologically interpretable information compared to gene or pathway based approaches.