• Journal of Microbiology and Biotechnology
• /
• v.27 no.2
• /
• pp.412-415
• /
• 2017

Prebiotics improve the growth or activities of specific microbial genera and species in the gut microbiota in order to confer health benefits to the host. In this study, we investigated the effect of poly-gamma-glutamate (${\gamma}-PGA$) as a prebiotic on the gut microbiota of mice and the organ distributions of ${\gamma}-PGA$ in mice. Pyrosequencing analysis for 16S rRNA genes of bacteria indicated that oral administration of ${\gamma}-PGA$ increased the abundance of Lactobacillales while reducing the abundance of Clostridiales in murine guts. It is suggested that oral administration of ${\gamma}-PGA$ can be helpful for modulating the gut microbiota as a prebiotic.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.17 no.2
• /
• pp.263-274
• /
• 2003

The present condition of study on the wave-resonance for the bio-KI is observed. And the probroms of reception attendent on the stimulation of wave is observed centering around the sensory organ. The results is as follows: In the wave-resonant stand point, the tendency of studying on KI is showed in the several field all over the world. Because it is originated radionics, the wave-resonant tools of MRA insistenting minute-magnetism-resonance-apparatus need the more severe data in the side of electric circuit. The wave resonance apparatus according to the frequency occurance transmits low-frequency's vibration ratio to the electric stimulating aspect. The wave-water is considered on the application of wave-resonance transcription on the water, and needs the comprehension of torsion-field level. The wave-stimulation of the bio-KI and the reception of the sensory-organ is observed the connection of the sensory-organ and it's corresponding wave. The informations recorded in the wave are distinguished patternly. And the several shaping waves transmit the informations each other through the resonnance. The wave theory is explained the LEE(理) as the wave-pattern and the KI(氣) as the revelation of the patterned-wave in the LEE-KI theory(理氣論), moreover the SHIN(神) as the information of the wave-pattern and the KI as the energy of the wave and the JEUNG(精) as the material of the wave in the JEUNG-KI-SHIN theory(精氣神論). In this point, the study on the wave-resonance of bio-KI is thought that it is significantly in the study method for oriental medicine. The sum of the wave makes the moving body and forms the universe. It means that the several wave patterns gather and form new field. The pattern is a kind of the information and the information is not materially. The information of wave-pattern is the arrangement and the combination of the material source.

• Proceedings of the Korean Society of Developmental Biology Conference
• /
• 2009.02a
• /
• pp.47-48
• /
• 2009

• Korean Journal of Poultry Science
• /
• v.45 no.3
• /
• pp.147-154
• /
• 2018

A total of 90 one-day-old male broilers (Ross 308) were randomly assigned to one of the three dietary treatments, each consisting of six replicates (5 broilers/cage). The dietary treatments were 1) control (CON: fresh clean water with no supplement); 2) low dose [LD: CON + 1.56% extractions from the wooden chips (EWC)] and 3) high dose (HD: CON + 12.5% EWC). Drinking water supplemented with EWC was provided using specifically designed individual nipple drinker units. Average daily water intake (ADWI), average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (FCR) were measured weekly for 21 days. One broiler from each cage was euthanized for measuring the visceral organ weights and collecting ileal tissue samples for ileal architecture analysis on day 21. Broilers assigned to the LD and HD watery groups showed higher ADWI than that in broilers consumed CON on day 7 (P<0.05). The broilers subjected to HD treatment showed a deeper crypt depth (P<0.05) than that in broilers subjected to LD and CON on day 21. Therefore, broilers consumed HD showed a lower (P<0.05) villus height:crypt depth ratio than that broilers consumed CON on day 21. Broilers provided drinking water containing any of the tested concentrations of EWC showed no effect (P > 0.05) on growth performance, ileal villus height, and visceral organ weights as compared with those in the CON from hatch to 21 days. In conclusion, broilers fed HD showed reduction in villus height:crypt depth ratio without impairing growth performance and visceral organ weights for the experimental period.

• Toxicological Research
• /
• v.24 no.1
• /
• pp.79-86
• /
• 2008

This study was conducted to obtain information of the oral dose toxicity of low molecular fucoidan (LMF) in male and female mice. In order to calculate 50% lethal dose ($LD_{50}$) and approximate lethal dose (LD), test material was once orally administered to male and female ICR mice at dose levels of 2000, 1000, 500, 250, 125 and 0 (vehicle control) mg/kg (body wt.). The mortality and the changes on body weight, clinical signs, gross observation and organ weight and histopathology of principle organs were monitored 14 days after LMF treatment. We could not find any mortalities, clinical signs, body weight changes and gross findings. In addition, significant changes in the organ weight and histopathology of principal organs were not observed except for some sporadic findings. The results obtained in this study suggest that LMF may not be toxic in mice and may be therefore safe for clinical use. The $LD_{50}$ and approximate LD in mice after single oral dose of LMF were considered over 2000 mg/kg in both female and male mice.

• Reproductive and Developmental Biology
• /
• v.29 no.3
• /
• pp.149-154
• /
• 2005

The purpose of this study was the analysis of sperm ability in Specific Pathogen Free (SPE) miniature pig for production of bio-organ. The collected semen was diluted with extender and stored at $17^{\circ}C$t for up to 7 days. The semen samples were evaluated at 0, 1, 3, 5, and 7 days of storage for analysis of sperm ability. Sperm ability was evaluated by examining viability, progressive motility, sperm abnormality and intensity of the sperm membrane. Also, the semen was processed according to the convenient freezing method, and frozen-thawed sperm was evaluated by examining viability, capacitation and acrosome reaction using chlortetracycline (CTC) staining. Motility of spermatozoa of SPF miniature pig was significantly (P<0.05) lower on 3 days or later compared to the Duroc, Yorkshire and Landrace in domestic boar. The percentage of abnormal spermatozoa of Landrace were significantly (P<0.05) higher than in SPF miniature pig, Duroc and Yorkshire that had a similar percentage on 5 or 7 days of sperm storage. The percentage of spermatozoa with coiled tail decreased during the storage period but there were no significant difference. On the other hand, viability of frozen-thawed spermatozoa had a significantly (P<0.05) lower in SPF miniature pig than in other domestic boars. CTC patterns had no significant difference, but SPF miniature pig had higher percentage of capacitated spermatozoa and lower percentage of acrosome-reacted it than domestic boars. Therefore, this study suggest that it is necessary to develop the suitable extender and freezing methods methods for the high viable rate and fertilizing ability in vitro.

• Journal of Plant Biotechnology
• /
• v.37 no.4
• /
• pp.539-548
• /
• 2010

Potassium ($K^+$) is one of the most abundant cations in higher plant. It comprises about 10% of plant dry weight and it plays roles in numerous functions such as osmo- and turgor regulation, charge balance of plasma membrane and control of stomata and organ movement. Several potassium transporters and potassium channels regulate $K^+$ homeostasis in response to $K^+$ uptake systems. In this review, we describe the biological, biochemical and physiological characteristics of shaker like potassium channels in higher plant. Especially, we searched the rice genome databases and analysized expressed genes, genome structures and protein domain characteristics of shaker like potassium channels.

• Journal of Convergence for Information Technology
• /
• v.9 no.10
• /
• pp.227-233
• /
• 2019

This study surveyed the dental hygiene and health science students in order to grasp the bioethics consciousness from June to July 2018 and to provide basic data for the development of the bioethics curriculum. Correlation between fetal right of life and artificial insemination awareness was significant in gender(r=0.241), religion(r=-0.176), and department(r=-0.160). Correlation between awareness of organ donation and organ transplantation was significant in grade(r=0.129), religion(r=-0.098), bio ethics educ ation(r=0.235). Correlation of euthanasia perception was significant in religion (r=0. 139), bioethics education(r=-0.157). As a result, To establish an ethical view that is desirable for health college students, Necessary to develop a curriculum and to sys tematically run education programs by analyzing the factors that affect biomedical ethics.

• International Journal of Advanced Culture Technology
• /
• v.1 no.1
• /
• pp.1-5
• /
• 2013

This network system for home care realized communication by the bio-physical signal, to convey physical rhythm. Four function of displacement had point of a Vision, Somatosensory, Vestibular and CNS. Bio-physical signal was decided to design a maximum points and minimum points with 0.01unit in reference level. Bio-physical signal was checked to compound physical condition of body posture for sensory organ. There detected a measurement of Vision, Somatosensory, Vestibular, CNS and BMI. The service of network system of home can be used to support a health care system for health assistant in health care center. It will expect to manage a physical parameter for network communication.

• Toxicological Research
• /
• v.23 no.1
• /
• pp.65-72
• /
• 2007

This study was conducted to obtain information of the oral dose acute toxicity of PGB-2, a novel polyglucosamine polymer produced from Citrobacter sp. BL-4 (a new strain) in male and female mice. Mortality, body weight changes, clinical signs were monitored during 14 days after single oral dose of test article at dose levels of 2000, 1000, 500, 250 and 125 ml/kg. Gross lesions, organ weight and histopathology of principal organs were examined after necropsy. As the results, we could not find any mortalities, clinical signs, changes in the body weight and gross findings except for white foci in the liver. In addition, no PGB-2-treatment related abnormal changes on the organ weight and histopathology of principle organs were detected except for atypical signs of liver. White liver foci were confirmed as focal infiltration of inflammatory cells. The results suggest that the PGB-2 is relatively safe in mice but the possibility of hepatotoxicity could not be excluded. The $LD_{50}$ and approximate LD in mice after single oral dose of PGB-2 were considered over 2000 mg/kg, respectively. In future, the potential hepatotoxicity of PGB-2 should be evaluated through the repeat dose toxicity test prior to develop as a new agent.