• Title/Summary/Keyword: Bio-formulation

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Development of High-Functional Hyaluronic Acid/Salmon Extract Formulation Using Gamma-Ray (감마선을 이용한 고기능성 히알루론산/연어추출물 제형개발)

  • Kweon, Dong-Keon;Shim, Jae-Goo;Ha, Man
    • Journal of the Korean Society of Radiology
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    • v.12 no.1
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    • pp.9-16
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    • 2018
  • Hangover after drinking is different from person to person symptoms and degree, but usually thirst, fatigue, headache, general boredom, gastrointestinal disorder, vomiting, diarrhea, deficiency of vitamin appears. This hanging phenomenon is caused by the action of precursors such as ethyl acetate and acetaldehyde, which are the by products of fermentation contained in alcohol and alcohol accumulated in hepatocytes and body. In order to solve the hangover phenomenon, the same Origin as polysaccharide Polydeoxyribonucleotide, which is a nucleic acid-sugar-phosphate complex, which is a semen or testicular extract in salmon extract, and a water soluble salmon extract powder having the same structure and lower price than Polydeoxyribonucleotide And D-Glucuronic acid and N-Acetyl glucosamine. It has excellent biocompatibility, viscoelasticity and moisturizing power. It has effect on reduction of body water loss and skin moisture content in hangover phenomenon. It is antioxidant and skin moisturizing effect Hyaluronic acid was irradiated with gamma rays, and the composition was prepared by using the salmon extract powder and the main raw material. The ethanol degradation, the acetaldehyde reduction amount, the blood acetaldehyde concentration and the acetic acid concentration were measured to evaluate the alcoholysis effect, Skin moisture evaporation rate To examine the evaluation unit water content of the skin was improved determine whether the antioxidant and provide skin moisturizing effect. The addition of ethanol extracts of salmon extracts showed a decrease of 5 to 7 times compared with no addition, and a decrease of 3 to 5 times of acetaldehyde. In addition, the change of acetaldehyde concentration and acetic acid concentration in blood showed a rapid decrease compared to the no - added control group. In addition, when the raw material of hyaluronic acid was used, skin moisture content was high and skin moisture evaporation amount was decreased. Therefore, hyaluronic acid, which is a polysaccharide polymer, has excellent viscoelasticity and moisturizing ability, It is considered to provide antioxidant and skin moisturizing effect. Therefore, it can be said that the composition containing salmon extract powder and hyaluronic acid as a main ingredient is effective for the hangover phenomenon which occurs after drinking.

Experimental Study on the Infiltration Loss in Plastic Greenhouses Equipped with Thermal Curtains (보온커튼을 설치한 플라스틱 온실의 틈새환기전열량 실측조사)

  • Nam, Sang-Woon;Shin, Hyun-Ho
    • Journal of Bio-Environment Control
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    • v.24 no.2
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    • pp.100-105
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    • 2015
  • The calculation method of infiltration loss in greenhouse has different ideas in each design standard, so there is a big difference in each method according to the size of greenhouses, it is necessary to establish a more accurate method that can be applied to the domestic. In order to provide basic data for the formulation of the calculation method of greenhouse heating load, we measured the infiltration rates using the tracer gas method in plastic greenhouses equipped with various thermal curtains. And then the calculation methods of infiltration loss in greenhouses were reviewed. Infiltration rates of the multi-span and single-span greenhouses were measured in the range of $0.042{\sim}0.245h^{-1}$ and $0.056{\sim}0.336h^{-1}$ respectively, single-span greenhouses appeared to be slightly larger. Infiltration rate of the greenhouse has been shown to significantly decrease depending on the number of thermal curtain layers without separation of single-span and multi-span. As the temperature differences between indoor and outdoor increase, the infiltration rates tended to increase. In the range of low wind speed during the experiments, changes of infiltration rate according to the outdoor wind speed could not find a consistent trend. Infiltration rates for the greenhouse heating design need to present the values at the appropriate temperature difference between indoor and outdoor. The change in the infiltration rate according to the wind speed does not need to be considered because the maximum heating load is calculated at a low wind speed range. However the correction factors to increase slightly the maximum heating load including the overall heat transfer coefficient should be applied at the strong wind regions. After reviewing the calculation method of infiltration loss, a method of using the infiltration heat transfer coefficient and the greenhouse covering area was found to have a problem, a method of using the infiltration rate and the greenhouse volume was determined to be reasonable.

Experimental Study on the Characteristics of Ground Heat Exchange in Heating Greenhouses (난방 온실의 지중열 교환 특성에 관한 실험적 연구)

  • Shin, Hyun-Ho;Nam, Sang-Woon
    • Journal of Bio-Environment Control
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    • v.25 no.3
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    • pp.218-223
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    • 2016
  • The calculation method of ground heat exchange in greenhouses has different ideas in each design standard, so there is a big difference in each method according to the size of greenhouses, it is necessary to establish a more accurate method that can be applied to the domestic. In order to provide basic data for the formulation of the calculation method of greenhouse heating load, we measured the soil temperature distribution and the soil heat flux in three plastic greenhouses of different size and location during the heating period. And then the calculation methods of ground heat exchange in greenhouses were reviewed. The soil temperature distributions measured in the heating greenhouse were compared with the indoor air temperature, the results showed that soil temperatures were higher than room temperature in the central part of greenhouse, and soil temperatures were lower than room temperature in the side edge of greenhouse. Therefore, it is determined that the ground heat gain in the central part of greenhouse and the perimeter heat loss in the side edge of greenhouse are occurred, there is a difference depending on the size of greenhouse. Introducing the concept of heat loss through the perimeter of building and modified to reflect the size of greenhouse, the calculation method of ground heat exchange in greenhouses is considered appropriate. It was confirmed that the floor heat loss measured by using soil heat flux sensors increased linearly in proportion to the temperature difference between indoor and outdoor. We derived the reference temperature difference which change the direction of ground heat flow and the perimeter heat loss factor from the measured heat flux results. In the heating design of domestic greenhouses, reference temperature differences are proposed to apply $12.5{\sim}15^{\circ}C$ in small greenhouses and around $10^{\circ}C$ in large greenhouses. Perimeter heat loss factors are proposed to apply $2.5{\sim}5.0W{\cdot}m^{-1}{\cdot}K^{-1}$ in small greenhouses and $7.5{\sim}10W{\cdot}m^{-1}{\cdot}K^{-1}$ in large greenhouses as design standard data.

Influence of Varied Pre-planting N Levels in a Medium on the Growth of Chinese Cabbage and Pak-choi Seedlings in Paper Pot Raising (종이포트 육묘시 기비로 혼합된 질소 시비수준이 배추와 청경채 생장에 미치는 영향)

  • Kim, Hyun Cheul;Park, Myong Sun;Jang, Yoonah;An, Sewoong;Choi, Jong Myung
    • Journal of Bio-Environment Control
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    • v.28 no.4
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    • pp.342-351
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    • 2019
  • The optimum N concentrations incorporated as pre-planting nutrient charge fertilizer were determined for seedling raising using cylindrical paper pots. A root medium was formulated by blending of peat moss (particles smaller than 2.84 mm were 80-90%) and perlite (1 to 3 mm) with the ratio of 7:3 (v/v). The treatment N concentrations incorporated during the root medium formulation were adjusted to 0, 150, 250, 500, and $750mg{\cdot}L^{-1}$ and the concentrations of essential nutrients except N were equal in all treatments. After making of paper pots and putting into the 40-cell tray, the seeds of Chinese cabbage ('Chunmyeong Bom Baechu') and pak-choi ('Hanog cheonggyeongchae') were sown. During the raising of seedlings, weekly analysis of medium pH, EC and concentrations of inorganic elements were conducted. After 21 and 20 days after seed sowing of Chinese cabbage and pak-choi, the growth of the above-ground parts were measured and contents of inorganic elements in the plant tissues were analyzed. During the growing period, pH of the root media rose gradually and the EC decreased rapidly at week 3. The pH of root media at harvest was in the range of 5.3 to 5.9 in Chinese cabbage and 4.93 to 5.39 in pak-choi. Growth of the aboveground parts in terms of fresh and dry weight in both the plants were the highest in the $250mg{\cdot}L^{-1}$ N treatment and the lowest in the control treatment. The elevation of pre-planting N concentrations in root medium resulted in the increase of tissue N content and decrease of P, Ca, and Mg contents. The regression equation derived from the influence of varied pre-planting N concentrations on dry weight of above-ground tissue were $y=-0.0036x^2+0.0021x+0.0635$ ($R^2=0.9826$) in Chinese cabbage and $y=-0.16x^2+0.0009x+0.032$ ($R^2=0.991$) in pak-choi. When the low critical concentration of pre-plant N is taken at the point where dry weight of above-ground tissue is 10% less than maximum (0.40 g in Chinese cabbage and 0.16 g in pak-choi), those point are 0.36 g and 0.144 g per plant in Chinese cabbage and pak-choi, respectively. The lower critical N concentrations of root media calculated from the regression equations are $196mg{\cdot}L^{-1}$ for Chinese cabbage and $187mg{\cdot}L^{-1}$ for pak-choi. These results indicate that optimum pre-plant N concentrations for seedling raising using paper pots are in the range of 196 to $250mg{\cdot}L^{-1}$ for Chinese cabbage and 187 to $250mg{\cdot}L^{-1}$ for pak-choi.

Quality and Fruit Productivity of the Second Truss Blooming Seedlings Depending on Concentration of Nutrient Solution in Cherry Tomato (양액 농도에 따른 방울토마토 2화방 개화묘의 소질 및 과실 생산성)

  • Lee, Mun Haeng
    • Journal of Bio-Environment Control
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    • v.31 no.3
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    • pp.230-236
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    • 2022
  • This study was carried out to produce two-flowered seedlings, harvest them early in a greenhouse, and extend the harvest period. This study was carried out to effectively produce the second truss blooming seedlings to harvest tomatoes early and extend the harvest period. For production of the second truss blooming seedlings (one stem), the nutrient solution EC was supplied at 1.5, 2.0, 2.5 dS·m-1, and dynamic management (3.0 → 3.5 → 4.5 dS·m-1). The seedling period was 60 days, which was 20-40 days longer than conventional seedlings, and 10 days longer than the first truss blooming seedlings (cube seedlings). The plant height was 78 and 77 cm in EC 2.5 dS·m-1 and dynamic management respectively, which was shorter than EC 1.5 dS·m-1 with 88 cm. As for the EC in the cube before formulation, dynamic management had the highest EC 5.5 dS·m-1, and the cube supplied with EC 1.5 dS·m-1 had the lowest. The production yield by treatment did not a difference among in the second truss blooming seedlings, but the first truss blooming seedlings showed lower productivity than second truss blooming seedlings. The second truss blooming seedling were harvested 35 days after planting on June 4, the first harvest date, and the first truss blooming were harvested in 42 days on June 11th. There was no difference in plant height and root growth due to bending at frequency planting. In the study on the production of the second truss blooming seedlings (two stem), the nutrient solution EC was supplied under 2.0, 2.5, 3.0 dS·m-1, and dynamic management (3.0 → 3.5 → 4.5 dS·m-1). The seedling period was 90 days, which was 40-50 days longer than conventional seedlings and 10 days longer than the first truss blooming seedlings (cube seedlings). Plant height was 80 and 81 cm in EC 2.0 dS·m-1 and 2.5 dS·m-1 respectively, but was the shortest at 73 cm in dynamic management. EC in the medium increased as the seeding period increased in all treatments. The dynamic management was the highest with EC 5.1 dS·m-1. There was no difference in yield among EC treatments in the second truss blooming seedlings, which had a longer seeding period of about 10 days, produced 15% more than the first truss blooming seedlings. In order to shorten the plant height of the second truss blooming seedlings, it is judged that the most efficient method is increasing the concentration of nutrient solution.

Development of Adhesive Resins Formulated with Rapeseed Flour Akali Hydrolyzates for Plywood Panels (유채박의 알칼리 가수분해물을 이용한 합판용 접착제의 개발)

  • Yang, In;Jeong, Jae-Hoon;Han, Gyu-Seong;Cho, In-Gyu;SaGong, Moon;Ahn, Sye-Hee;Oh, Sei-Chang
    • Journal of the Korean Wood Science and Technology
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    • v.38 no.4
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    • pp.323-332
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    • 2010
  • Petroleum-based adhesive resins have extensively been used for the production of wood panels. However, it is necessary to develop environmentally friendly adhesive resins due to the increase of manufacturing cost and the environmental issue, such as the emission of volatile organic compounds, of the pertroleum-based adhesive resins. This study was conducted to formulate environmentally friendly adhesive resins using by rapeseed flour (RSF), which is the by-product of bio-diesel produced from rapeseed, for replacing petroleum-based adhesives with them. To formulate RSF-based adhesive resins, RSF was hydrolyzed in de-ionized water, 1% and 3% sodium hydroxide solutions. As a crosslinking agent, PF prepolymers were prepared with 1.8, 2.1 and 2.4 mol formaldehyde and 1 mol phenol (1.8-, 2.1- and 2.4-PF), and then mixed with RSF hydrolyzates to complete the formulation of RSF-based adhesive resins. The RSF-based adhesive resins were applied to fabricate 3-ply plywood panels. The solid content of RSF-based adhesive resins were ranged from 26.08% to 36.12% depending on the hydrolysis condition of RSF and PF prepolymer type with a high viscosity. The tensile shear strength and wood failure of plywood fabricated with RSF-based adhesive resins exceeded a minimum requirement of KS standard for ordinary plywood regardless of the hydrolysis condition of RSF and PF prepolymer type. Formaldehyde emissions of the plywood panels fabricated with 1.8-PF and RSF hydrolyzates were lower than that of E0 specified in the KS standard. Based on the results, RSF might be used as a raw material of environmentally friendly adhesives for the production of plywood panels, but further researches - the increase of solid content of RSF-based adhesives for reducing press time and the microscopic observation of plywood specimen for identifying the relationship between tensile shear strength and the penetration of adhesives into wood structure - are required to commercialize the RSF-based adhesives.

Characterization of Biopesticides (Bacillus thuringiensis) Produced in Korea (국내에서 생산된 Bacillus thuringiensis 살충제의 특성)

  • Kil, Mi-Ra;Kim, Da-A;Choi, Su-Yeon;Paek, Seung-Kyoung;Kim, Jin-Su;Jin, Da-Yong;Hwang, In-Chon;Yu, Yong-Man
    • The Korean Journal of Pesticide Science
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    • v.11 no.3
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    • pp.201-209
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    • 2007
  • Characteristics of the 5 biopesticides that included Bacillus thuringiensis and on the domestic markets were investigated. These products were contained different strains of B. thuringiensis, for examples; product A and E was B. thuringiensis subsp aizawai; product B was B. thuringiensis; product C was B. thuringiensis Berline var. kurstaki; product D was B. thuringiensis var. kurstaki. Number of active spores were counted because they could influence the bio-activity against target pests. Only product C are contained the fixed quantity as its label, however, product D and E were a tenth part, and product A and B were a hundredth part of their descriptions. The pHs of product A and B were measured 3.67 and 3.73, and C, D and E were 5, respectively. Typical bypyramidal crystals produced from B. thuringiensis was found in only product C under a phase contrast microscope. For the uniform formulation of products that conformed whether B. thuringiensis were equally spreaded on the crops, B. thuringiensis in the C, D and E were equally grown on the nutrient agar medium As a results, product A were more different from product C than any other products. When product A and C were bioassayed against different larval stages of diamondback moth, their mortalities with spraying application were showed 100% after 48 hours.

Risk Assessment of Operator Exposure During Treatment of Fungicide Dithianon on Apple Orchard (사과 과수원에서 농약살포시 살균제 Dithianon의 농작업자 위해성 평가)

  • Cho, ll Kyu;Kim, Su Jin;Kim, Ji Myung;Oh, Young Goun;Seol, Jae Ung;Lee, Ji Ho;Kim, Jeong Han
    • Korean Journal of Environmental Agriculture
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    • v.37 no.4
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    • pp.302-311
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    • 2018
  • BACKGROUND: Dithianon (75%) formulation were mixed and sprayed as closely as possible by normal practice on the ten farms located in the Mungeong of South Korea. Patches, cotton gloves, socks, masks, and XAD-2 resin were used for measurement of the potential exposure of dithianon on the applicators wearing standardized whole-body outer and inner dosimeter (WBD). This study has been carried out to determine the dermal and inhalation exposure to dithianon during preparation of spray suspension and application with a power sprayer on a apple orchard. METHODS AND RESULTS: A personal air monitor equipped with an air pump, IOM sampler and cassette, and glass fiber filter was used for inhalation exposure. The field studies were carried out in a apple orchard. The temperature and relative humidity were monitored with a thermometer and a hygrometer. Wind speed was measured using a pocket weather meter. All mean field fortification recoveries were between 85.1% and 99.1% in the level of 100 LOQ (limit of quantification), while the LOQ for dithianon was $0.05{\mu}g/mL$ using HPLC-DAD. The exposure to dithianon on arms of the mixer/loader (0.0794 mg) was higher than other body parts (head, hands, upper body, or legs). The exposure to dithianon on the applicator's legs (3.78 mg) was highest in the body parts. The dermal exposures for mixer/loader and applicator were 10 and 8.10 mg, respectively, from a grape orchard. The inhalation exposure during application was estimated as 0.151 mg, and the ratio of inhalation exposure was 11.2% of the dermal exposure (inner clothes). CONCLUSION: The dermal and inhalation exposure on the applicator appeared to be 4.203 mg - 25.064 mg and $0.529{\mu}g-116.241{\mu}g$, respectively. The total exposures on the agricultural applicators were at the level of 2.596 mg - 25.069 mg to dithianon during treatment for apple orchard. The TER showed 3.421 (>1) when AOEL of dithianon was used as a reference dose for the purpose of risk assessment of the mixing/loading and application.