• Title/Summary/Keyword: Bio-control

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Sampling and Selection Factors that Enhance the Diversity of Microbial Collections: Application to Biopesticide Development

  • Park, Jun-Kyung;Lee, Seung-Hwan;Lee, Jang-Hoon;Han, Songhee;Kang, Hunseung;Kim, Jin-Cheol;Kim, Young Cheol;McSpadden Gardener, Brian
    • The Plant Pathology Journal
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    • 제29권2호
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    • pp.144-153
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    • 2013
  • Diverse bacteria are known to colonize plants. However, only a small fraction of that diversity has been evaluated for their biopesticide potential. To date, the criteria for sampling and selection in such bioprospecting endeavors have not been systematically evaluated in terms of the relative amount of diversity they provide for analysis. The present study aimed to enhance the success of bioprospecting efforts by increasing the diversity while removing the genotypic redundancy often present in large collections of bacteria. We developed a multivariate sampling and marker-based selection strategy that significantly increase the diversity of bacteria recovered from plants. In doing so, we quantified the effects of varying sampling intensity, media composition, incubation conditions, plant species, and soil source on the diversity of recovered isolates. Subsequent sequencing and high-throughput phenotypic analyses of a small fraction of the collected isolates revealed that this approach led to the recovery of over a dozen rare and, to date, poorly characterized genera of plant-associated bacteria with significant biopesticide activities. Overall, the sampling and selection approach described led to an approximately 5-fold improvement in efficiency and the recovery of several novel strains of bacteria with significant biopesticide potential.

바이오 황의 노지 감귤 검은점무늬병 및 귤응애에 대한 효과 (Effects of Using Bio-Sulfur on Open Field Citrus Melanose (Diaporthe citri) and Panonychus citri)

  • 오명협;박원표;현해남
    • 한국환경과학회지
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    • 제29권10호
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    • pp.997-1002
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    • 2020
  • In this study, we investigated the effects of the eco-friendly chemical bio-sulfur, on the citrus melanose-causing pathogen, Diaporthe citri, and on the pest, Panonychus citri. In an open field experiment with a plot-scale application of the chemicals: Mancozeb, lime sulfur, lime sulfur + machine oil, and bio-sulfur, the control group showed 70.6% disease severity compared with 10.3% for the Mancozeb-treated group. Among the eco-friendly treatments, disease severity was the lowest for the group treated with lime sulfur + machine oil (32.2%) and was 53.9%, 58.8%, and 58.1% following treatment with lime sulfur, and bio-sulfur diluted 500 and 1000 times, respectively. The proportion of diseased fruit showed similar results, suggesting that bio-sulfur is an effective alternative to lime sulfur. Three days after treatment acaricidal effects on P. citri showed a 197.6% control survival rate whereas the machine oil, and bio-sulfur diluted 500 and 1000 times treatments showed rates of 2.9%, 5.8%, and 9.0%, respectively. After three days, the control value for bio-sulfur diluted 1000 times was 73.2% compared with the values for the machine oil (96.4%) and bio-sulfur diluted 500 times (94.6%) treatments. Therefore, we suggest that additional research is needed on the combined application of bio-sulfur and oils to enhance the additive control effect on citrus melanose and Panonychus citri.

21C 시설원예산업의 발전 방향

  • 김목원
    • 한국생물환경조절학회:학술대회논문집
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    • 한국생물환경조절학회 2000년도 학술발표논문집 Proceeding of Bio-Environment control
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    • pp.1-10
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    • 2000
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친환경농업과 농약

  • 정봉진
    • 한국생물환경조절학회:학술대회논문집
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    • 한국생물환경조절학회 2005년도 세계의 친환경 시설원에 기술동향 및 춘계 국제학술대회 발표논문집
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    • pp.3-8
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    • 2005
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친환경농업과 비료

  • 박범기
    • 한국생물환경조절학회:학술대회논문집
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    • 한국생물환경조절학회 2005년도 세계의 친환경 시설원에 기술동향 및 춘계 국제학술대회 발표논문집
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    • pp.9-43
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    • 2005
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친환경 시설채소 생산과 상품화 방안

  • 서범석
    • 한국생물환경조절학회:학술대회논문집
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    • 한국생물환경조절학회 2005년도 세계의 친환경 시설원에 기술동향 및 춘계 국제학술대회 발표논문집
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    • pp.44-78
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    • 2005
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