• 한국포장학회지
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• 제23권3호
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• pp.183-190
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• 2017

Carton clamp truck is widely perceived as the high-efficient handling equipment of factory premises and warehouse by its capability of palletless handling. Therefore, the significance of a lab-based handling simulation is becoming higher with the growth of clamp truck usage. In this study, preliminary FEA and design of handling test courses for the lab-based simulation of carton clamp truck handling were performed, and the PSD analyses were performed for the modified one for the test course proposed by Park et al. (2017) as well as ASTM D 6055 and ISTA 3B standards. For the vibration in all directions, the vibration energy intensity analyzed by ISTA 3B standard showed higher than that by the other two cases. A FEA was performed for the handling operation of the sudden stop of the clamps after lifting the target HCP (heavyweight refrigerator corrugated package, w=180 kgf) up to the specified height. The slip distance between the clamp arm and the target HCP was 0.85 mm. The simulation result of 0.85 mm was 3.7 times lower than the experimental result (3.2 mm) obtained by Park et al. (2017), and it was estimated that the deviation comes from both the experimental error by weight imbalance of target HCP, and excessive simplification during the FE modelling of target HCP.

• Journal of Microbiology and Biotechnology
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• 제15권2호
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• pp.254-258
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• 2005

We have conducted in vitro reconstitution study of ferritin from its subunits FerH and FerL. For the reconstitution, FerH was produced from an expression vector construct in Escherichia coli and was purified from a heat treated cell extract by using one-step column chromatography. FerL was expressed as inclusion bodies. The denatured form of FerL was obtained by a simple washing step of the inclusion bodies with 3 M urea. The reconstitution experiment was conducted with various molar ratios of urea-denatured FerH and FerL to make the ferritin nanoparticle with a controlled composition of FerH and FerL. SDS-PAGE analysis of the reconstituted ferritins revealed that the reconstitution required the presence of more than 40 molar$\%$ of FerH in the reconstitution mixture. The assembly of the subunits into the ferritin nanoparticle was confmned by the presence of spherical particles with diameter of 10 nm by the atomic force microscopic image. Further analysis of the particles by using a transmission electron microscope revealed that the reconstituted particles exhibited different percentages of population with dense iron core. The reconstituted ferritin nanoparticles made with molar ratios of [FerH]/[FerL]=l00/0 and 60/40 showed that 80 to $90\%$ of the particles were apoferritin, devoid of iron core. On the contrary, all the particles formed with [FerH]/[FerL]=85/ 15 were found to contain the iron core. This suggests that although FerH can uptake iron, a minor portion of FerL, not exceeding $40\%$ at most, is required to deposit iron inside the particle.

• Journal of Microbiology and Biotechnology
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• 제15권3호
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• pp.502-509
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• 2005

Sexual reproduction plays an important role in the biology and epidemiology of oomycete plant pathogens such as the heterothallic species Phytophthora infestans. Recent worldwide dispersal of A2 mating type strains of P. infestans resulted in increased virulence, gene transfer, and genetic variation, creating new challenges for disease management. To develop a genetic assay for mating type identification in P. infestans, we used the Amplified Fragment Length Polymorphism (AFLP) technique. The primer combination E+AT/M+CTA detected a fragment specific to A1 mating type (Mat-A1) of P. infestans. This fragment was cloned and sequenced, and a pair of primers (INF-1, INF-2) were designed and used to differentiate P. infestans Mat-A1 from Mat-A2 strains. The Mat A1-specific fragment was detected using Southern blot analysis of PCR products amplified with primers INF-1 and INF-2 from genomic DNA of 14 P. infestans Mat-A1 strains, but not 13 P. infestans Mat-A2 strains or 8 other isolates representing several Phytophthora spp. Southern blot analysis of genomic DNAs of P. infestans isolates revealed a 1.6 kb restriction enzyme (EcoRI, BamHI, AvaI)-fragment only in Mat-A1 strains. The A1 mating type-specific primers amplified a unique band under stringent annealing temperatures of $63^{\circ}C-64^{\circ}C$, suggesting that this PCR assay could be developed into a useful method for mating type determination of P. infestans in field material.

• The Journal of Advanced Prosthodontics
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• 제6권6호
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• pp.528-538
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• 2014

PURPOSE. The purpose of this prospective study was to evaluate the effectiveness of newly developed autogenous tooth bone graft material (AutoBT)application for sinus bone graft procedure. MATERIALS AND METHODS. The patients with less than 5.0 mm of residual bone height in maxillary posterior area were enrolled. For the sinus bone graft procedure, Bio-Oss was grafted in control group and AutoBT powder was grafted in experimental group. Clinical and radiographic examination were done for the comparison of grafted materials in sinus cavity between groups. At 4 months after sinus bone graft procedure, biopsy specimens were analyzed by microcomputed tomography and histomorphometric examination for the evaluation of healing state of bone graft site. RESULTS. In CT evaluation, there was no difference in bone density, bone height and sinus membrane thickness between groups. In microCT analysis, there was no difference in total bone volume, new bone volume, bone mineral density of new bone between groups. There was significant difference trabecular thickness ($0.07{\mu}m$ in Bio-Oss group Vs. $0.08{\mu}m$ in AutoBT group) (P=.006). In histomorphometric analysis, there was no difference in new bone formation, residual graft material, bone marrow space between groups. There was significant difference osteoid thickness ($8.35{\mu}m$ in Bio-Oss group Vs. $13.12{\mu}m$ in AutoBT group) (P=.025). CONCLUSION. AutoBT could be considered a viable alternative to the autogenous bone or other bone graft materials in sinus bone graft procedure.

• Molecules and Cells
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• 제25권3호
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• pp.358-367
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• 2008

The embryonic germ cell (EGCs) of mice is a kind of pluripotent stem cell that can be generated from pre- and post-migratory primordial germ cells (PGCs). Most previous studies on DNA methylation of EGCs were restricted to 12.5 days post coitum (dpc). This study was designed to establish and characterize murine EGC lines from migrated PGCs as late as 13.5 dpc and to estimate the degrees of methylation of their imprinted genes as well as of the non-imprinted locus, Oct4, using an accurate and quantitative method of measurement. We established five independent EGC lines from post migratory PGCs of 11.5-13.5 dpc from C57BL/6 ${\times}$ DBA/2 F1 hybrid mouse fetuses. All the EGCs exhibited the typical features of pluripotent cells including hypomethylation of the Oct4 regulatory region. We examined the methylation status of three imprinted genes; Igf2, Igf2r and H19 in the five EGC lines using bisulfite genomic sequencing analysis. Igf2r was almost unmethylated in all the EGC lines irrespective of the their sex and stage of isolation; Igf2 and H19 were more methylated than Igf2r, especially in male EGCs. Moreover, EGCs derived at 13.5 dpc exhibited higher levels of DNA methylation than those from earlier stages. These results suggest that in vitro derived EGCs acquire different epigenotypes from their parental in vivo migratory PGCs, and that sex-specific de novo methylation occurs in the Igf2 and H19 genes of EGCs.

• Environmental Engineering Research
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• 제25권3호
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• pp.290-298
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• 2020

A large residual fraction of aliphatic components of diesel prevails in soil, which has adverse effects on the environment. This study identified the most bio-recalcitrant aliphatic residual fraction of diesel through total petroleum-hydrocarbon fractional analysis. For this, the strain Acinetobacter sp. K-6 was isolated, identified, and characterized and investigated its ability to degrade diesel and n-alkanes (C₁₈, C₂₀, and C₂₂). The removal efficiency was analysed after treatment with bacteria and nutrients in various soil microcosms. The fractional analysis of diesel degradation after treatment with the bacterial strains identified C₁₈-C₂₂ hydrocarbons as the most bio-recalcitrant aliphatic fraction of diesel oil. Acinetobacter sp. K-6 degraded 59.2% of diesel oil and 56.4% of C₁₈-C₂₂ hydrocarbons in the contaminated soil. The degradation efficiency was further improved using a combinatorial approach of biostimulation and bioaugmentation, which resulted in 76.7% and 73.7% higher degradation of diesel oil and C₁₈-C₂₂ hydrocarbons, respectively. The findings of this study suggest that the removal of mid-length, non-volatile hydrocarbons is affected by the population of bio-degraders and the nutrients used in the process of remediation. A combinatorial approach, including biostimulation and bioaugmentation, could be used to effectively remove large quantities of aliphatic hydrocarbons persisting for a longer period in the soil.

• 농업과학연구
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• 제45권4호
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• pp.839-845
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• 2018

As a solution to the rural shortage of labor, mechanization crop production is necessary, but in some cases, the mechanization can cause problems such as a decrease in products due to the expansion of the necessary moderate workspace. The purpose of this study was to compare the yields of pepper by the planting-density for the mechanization of pepper cultivation. Experiments were done with three planting-density levels of $900{\times}300mm^2$(A-T), $1200{\times}450mm^2$(B-T), and $1500{\times}600mm^2$(C-T). In the analysis of growth, the highest values in plant height and thickness and the number of branches were observed with the B-T. C-T showed the highest values in the number of green-pepper and red-pepper and weight of the green-pepper and red-pepper, followed by B-T and A-T. In the analysis of growth, it was concluded that the proportion of the pepper body to the total length increased as the planting-density decreased. C-T had the biggest maximum diameter of the body, followed by B-T and A-T. On the other hand, A-T had the biggest minimum diameter of the body, followed by B-T and C-T. It was judged that the larger the planting-density was, the shorter the length was and the thicker the form was. As a result of measuring the chromaticity, there was no significant statistical difference in quality. Based on the experiment results, the ranking in total yields was in the order of C-T, B-T, and A-T. The reduced planting-density seemed to increase the productivity, while the labor intensity and time were reduced due to the improvement of the working environment.

• 반도체디스플레이기술학회지
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• 제20권4호
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• pp.89-95
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• 2021

As the influence of fine dust on society spreads gradually, the public's interest in indoor air is increasingly rising. Air-purifying plants are drawing keen attention due to their natural purifying function enabled by plant physiology. However, as their fine dust reduction mechanism is limited to adsorption only, vegetation bio-filters that optimize purification effects through integration with air-conditioning systems is rising as an alternative. In accordance with the relevant standard test methods, this study looked into the fine dust reduction assessment method by air-conditioning airflow volume that can be used for the industrial spread of vegetation bio-filters. In the case of PM₁₀ at 300 ㎍/m³, it was in the order of EG-B(3,500CMH, 29 min.) < EG-A (2,500CMH, 37 min.) < CG(0CMH, 64 min.) for reaching the maintenance level (100 ㎍/m³) of publicly used facilities. For reaching the WHO Guideline(50 ㎍/m³) requirement, it was in the order of EG-B (51 min.) < EG-A (160 min.) < CG (170 min.). In the case of PM_2.5, it was in the order of EG-B (26 min.) < EG-A (33 min.) < CG (57 min.) for reaching the maintenance level (50 ㎍/m³) of publicly used facilities. It was in the order of EG-B (48 min) < EG-A (140 min) < CG (158 min) for reaching the WHO Guideline (25 ㎍/m³) requirement. The findings from the analysis showed that fine dust can be reduced most efficiently when the system is operated at 3,500CMH level. The limitation of this study is that due to the absence of a way of assessing the stress of plants in vegetation bio-filters, generating optimal air-conditioning air flow of the relevant system and economics analysis against the existing facility-type air purification system have been clarified, which should be explored further though follow-up studies.

• 한국전문물리치료학회지
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• 제30권3호
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• pp.230-236
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• 2023

Background: The globalization of the healthcare industry and the increasing demand for skilled professionals in the global healthcare industry have opened up opportunities for specialized biotech healthcare professionals to seek overseas employment and career advancement. Objects: This study aimed to develop educational content essential for the overseas employment of digital bio-health professionals. Methods: A survey was conducted among 196 participants. Google Forms (Google) were utilized to create and administer the survey, employing purposive sampling, a non-probability sampling method. Data analysis was performed using IBM SPSS 25.0 (IBM Co.), including Cronbach's α and independent sample t-tests to assess significant differences. Results: About half of college students are interested in overseas employment and international careers, while the other half had not. The most common reason for wanting to work or go overseas was "foreign experience will be useful for future activities in Korea." Students who had experience taking courses from the Bio-health Convergence Open Sharing University preferred overseas programs more than those who did not have that experience. In terms of the degree of desire for overseas education courses provided by universities, contents related to human health were the highest, followed by bio-health big data. Conclusion: Many students wanted to work and go overseas if there is sufficient support from the university. The findings in this study suggest that universities are necessary to play an important role in supporting students' aspirations to work or go overseas by providing language education, education and training programs, information on overseas jobs, and mentoring programs.

• 한국전자통신학회논문지
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• 제19권4호
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• pp.701-708
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• 2024

딥러닝 분야에서 트랜스포머 아키텍쳐의 출현은 자연어 처리 연구가 획기적인 발전을 가져왔다. 개체명 인식은 자연어 처리의 한 분야로 정보 검색과 같은 태스크에 중요한 연구 분야이다. 생의학 분야에서도 그 중요성이 강조되나 학습용 한국어 생의학 말뭉치의 부족으로 AI를 활용한 한국어 임상 연구 발전에 제약이 되고 있다. 본 연구에서는 한국어 생의학 개체명 인식을 위해 새로운 생의학 말뭉치를 구축하고 대용량 한국어 말뭉치로 사전 학습된 언어 모델들을 선정하여 전이 학습시켰다. F1-score로 선정된 언어 모델의 개체명 인식 성능과 태그별 인식률을 비교하고 오류 분석을 하였다. 인식 성능에서는 KlueRoBERTa가 상대적인 좋은 성능을 보였다. 태깅 과정의 오류 분석 결과 Disease의 인식 성능은 우수하나 상대적으로 Body와 Treatment는 낮았다. 이는 문맥에 기반하여 제대로 개체명을 분류하지 못하는 과분할과 미분할로 인한 것으로, 잘못된 태깅들을 보완하기 위해서는 보다 정밀한 형태소 분석기와 풍부한 어휘사전 구축이 선행되어야 할 것이다.