• 제목/요약/키워드: Bio-analysis

검색결과 3,811건 처리시간 0.03초

Requirement Analysis for Bio-Information Integration Systems

  • Lee, Sean;Lee, Phil-Hyoun;Dokyun Na;Lee, Doheon;Lee, Kwanghyung;Bae, Myung-Nam
    • 한국지능시스템학회:학술대회논문집
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    • 한국퍼지및지능시스템학회 2003년도 ISIS 2003
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    • pp.11-15
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    • 2003
  • Amount of biological data information has been increasing exponentially. In order to cope with this bio-information explosion, it is necessary to construct a biological data information integration system. The integration system could provide useful services for bio-application developers by answering general complex queries that require accessing information from heterogeneous bio data sources, and easily accommodate a new database into the integrated systems. In this paper, we analyze architectures and mechanisms of existing integration systems with their advantages and disadvantages. Based on this analysis and user requirement studies, we propose an integration system framework that embraces advantages of the existing systems. More specifically, we propose an integration system architecture composed of a mediator and wrappers, which can offer a service interface layer for various other applications as well as independent biologists, thus playing the role of database management system for biology applications. In other words, the system can help abstract the heterogeneous information structures and formats from the application layer. In the system, the wrappers send database-specific queries and report the result to the mediator using XML. The proposed system could facilitate in silico knowledge discovery by allowing combination of numerous discrete biological information databases.

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Comparative Analysis of Completely Sequenced Insect Mitochondrial Genomes

  • Lee, Jin-Sung;Kim, Ki-Hwan;Suh, Dong-Sang;Park, Jae-Heung;Suh, Ji-Yoeun;Chung, Kyu-Hoi;Hwang, Jae-Sam
    • International Journal of Industrial Entomology and Biomaterials
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    • 제2권1호
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    • pp.1-6
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    • 2001
  • This paper reports a few characteristics of seven insect mitochondrial genomes sequenced completely (Bombyx mori, Drosophila melanogaster, D. yakuba, Apis mellifera, Anopheles gambiae, A. quadrimaculatus, and Locusta migratoria). Comparative analysis of complete mt genome sequences from several species revealed a number of interesting features (base composition, gene content, A+T-rich region, and gene arrangement, etc) of insect mitochondrial genome. The properties revealed by our work shed new light on the organization and evolution of the insect mitochondrial genome and more importantly open up the way to clearly aimed experimental studies for understanding critical roles of the regulatory mechanisms (transcription and translation) in mitochondrial gene expression.

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농촌마을의 유형 구분을 위한 평가지표의 중요도 분석 연구 (An Analysis of Importance Weight of Evaluation Indicators for Classification of Rural Village)

  • 김영택;최수명;조은정;김홍균;임상봉
    • 농촌계획
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    • 제20권3호
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    • pp.121-130
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    • 2014
  • This study aimed at setting up the evaluation indicators system by rural village types to identify systematically the multi-valuedness embedded in rural villages. AHP(Analytic Hierarchy Process) was used for evaluating the relative importance weight evaluation of each indicator and quantitative analysis of rural village through computer works. The importance weight of evaluation indicators was converted into the score on the basis of maximum 1,000 point to increase the practicality. As a result, characteristics of 5 rural village types(Basic life-supporting, Agricultural promotion, Marketing/processing oriented, Urban-rural communication, Life-style choice types) differed in score of classified indicators. Also, These results are expected to be possible to quantitatively evaluate characteristics by rural village types.

Zinc Metal Solubilization by Gluconacetobacter diazotrophicus and Induction of Pleomorphic Cells

  • Saravanan, Venkatakrishnan Sivaraj;Osborne, Jabez;Madhaiyan, Munusamy;Mathew, Lazar;Chung, Jong-Bae;Ahn, Ki-Sup;Sa, Tong-Min
    • Journal of Microbiology and Biotechnology
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    • 제17권9호
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    • pp.1477-1482
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    • 2007
  • Gluconacetobacter diazotrophicus strain PA15 exhibited a minimum inhibitory concentration value of 11 mM in an LGI medium amended with $ZnCl_2$. When an LGI medium was amended with Zn metal, solubilization halos were observed in a plate assay, and further solubilization was confirmed in a broth assay. The maximum solubilization was recorded after 120 h with a 0.1% Zn metal amendment. During solubilization, the culture growth and pH of the broth were indirectly correlated. Using a Fourier Transform Infrared Spectroscopy analysis, one of the agents solubilizing the Zn metal was identified as gluconic acid. When the Zn-amended broth was observed under a bright field microscope, long involution cells were observed, and further analysis with Atomic Force Microscopy revealed highly deformed, pleomorphic, aggregate-like cells.

Gene Expression Analysis of Pregnant Specific Stage in the Miniature Pig Ovary

  • Yun, Seong-Jo;Noh, Won-Gun;Yoon, Jong-Taek;Min, Kwan-Sik
    • Reproductive and Developmental Biology
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    • 제33권4호
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    • pp.249-255
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    • 2009
  • The miniature pig is considered to be a better organ donor breed for xenotransplantation than other pig breeds because the size of the organs of the miniature pig is similar to that of humans. In this study, we aimed at identifying differentially expressed genes in the miniature pig ovary during pregnancy. For this, we used the miniature pig ovary model, annealing control primer-based reverse transcription polymerase chain reaction (PCR), quantitative real-time PCR (qRT-PCR), and northern blotting analysis. We identified 13 genes showing differential expression on the based of pregnancy status and validated 8 genes using qRT-PCR. We also sequenced the full-length cDNA of ephrin receptor A4 (EphA4), which had a significant difference in expression level, and validated it by northern blotting. These genes may provide a better understanding of the cellular and molecular mechanisms during pregnancy in miniature pig ovary.

Bio-inspired neuro-symbolic approach to diagnostics of structures

  • Shoureshi, Rahmat A.;Schantz, Tracy;Lim, Sun W.
    • Smart Structures and Systems
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    • 제7권3호
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    • pp.229-240
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    • 2011
  • Recent developments in Smart Structures with very large scale embedded sensors and actuators have introduced new challenges in terms of data processing and sensor fusion. These smart structures are dynamically classified as a large-scale system with thousands of sensors and actuators that form the musculoskeletal of the structure, analogous to human body. In order to develop structural health monitoring and diagnostics with data provided by thousands of sensors, new sensor informatics has to be developed. The focus of our on-going research is to develop techniques and algorithms that would utilize this musculoskeletal system effectively; thus creating the intelligence for such a large-scale autonomous structure. To achieve this level of intelligence, three major research tasks are being conducted: development of a Bio-Inspired data analysis and information extraction from thousands of sensors; development of an analytical technique for Optimal Sensory System using Structural Observability; and creation of a bio-inspired decision-making and control system. This paper is focused on the results of our effort on the first task, namely development of a Neuro-Morphic Engineering approach, using a neuro-symbolic data manipulation, inspired by the understanding of human information processing architecture, for sensor fusion and structural diagnostics.

이스라엘 잉어(Cyprinus carpio)에서 Aeromonas veronii 감염증의 증례: phylogenetic analysis와 항생제 내성 (A case of Aeromonas veronii infection in Israeli carp (Cyprinus carpio): phylogenetic analysis and antimicrobial resistance)

  • 이승원;유명조;이해범;신기욱
    • 한국동물위생학회지
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    • 제35권3호
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    • pp.239-243
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    • 2012
  • We reported an outbreak of Aeromonas (A.) veronii responsible for ulcerative dermatitis in Israeli carp (Cyprinus carpio). The major clinical signs were darkening body, abdominal extension, exophthalmos and severe ulcerative necrosis in the skin. The necropsy showed yellowish ascites, necrosis in liver and enlargement of kidney and spleen in the morbid fish. In blood agar for culturing bacteria, three different colonies were identified as A. veronii, Plesimonas shigelloides and Shewanella putrefaciens by phylogenetic identification using 16S rRNA or gyrB gene sequences. A. veronii was the most dominant species among them and was resistant to ampicillin, nalidixic acid and oxytetracycline.

정신건강 치유를 위한 치유농장 서비스품질의 중요도-만족도 분석(IPA) (Importance-Performance Analysis (IPA) of Agro-Healing Services Quality for Mental Health Care)

  • 최나윤;김선옥;김윤진;박신애
    • 농촌지도와개발
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    • 제28권4호
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    • pp.203-213
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    • 2021
  • This study aimed to examine the policy priorities to enhance the service quality of the agro-healing for mental health care using Importance-Performance Analysis (IPA). The subjects were 67 people working in mental health institutions. The result of the IPA for the design of the agro-healing policy, in a total of 5 policy areas, the importance and performance were found to be 'above average', and it was confirmed that 'Keep up the Good Work' level. This is thought to have had a positive effect on raising the policy awareness of experts in mental health related institutions as the government's support for agro-healing and R & D increased according to the enactment of the act. Therefore, the results of this study provided basic information on the development and revitalization of agro-healing service quality for mental health care.

Growth Characteristics of Polyporales Mushrooms for the Mycelial Mat Formation

  • Bae, Bin;Kim, Minseek;Kim, Sinil;Ro, Hyeon-Su
    • Mycobiology
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    • 제49권3호
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    • pp.280-284
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    • 2021
  • Mushroom strains of Polyporales from the genera Coriolus, Trametes, Pycnoporus, Ganoderma, and Formitella were explored in terms of mycelial growth characteristics for the application of mushroom mycelia as alternative sources of materials replacing fossil fuel-based materials. Among the 64 strains of Polyporales, G. lucidum LBS5496GL was selected as the best candidate because it showed fast mycelial growth with high mycelial strength in both the sawdust-based solid medium and the potato dextrose liquid plate medium. Some of the Polyporales in this study have shown good mycelial growth, however, they mostly formed mycelial mat of weak physical strength. The higher physical strength of mycelial mat by G. lucidum LBS5496GL was attributed to its thick hyphae with the diameter of 13 mm as revealed by scanning electron microscopic analysis whereas the hyphae of others exhibited less than 2 mm. Glycerol and skim milk supported the best mycelial growth of LBS5496GL as a carbon and a nitrogen source, respectively.

인삼토양으로부터 옥신 생성 식물생장촉진세균의 분리 및 특성 (Isolation and Characterization of the Auxin producing Plant Growth Promoting Rhizobacterium from Soil in a Ginseng Field)

  • 박해성;정영필;윤민호
    • 농업과학연구
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    • 제37권3호
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    • pp.377-382
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    • 2010
  • An auxin-producing bacterium (AMT-54) was isolated from ginseng cultivating soil of Geumsan area. The isolate AMT-54 was confirmed to produce indole-3-acetic acid (IAA) which is one of auxin hormone by TLC analysis. When the concentration of IAA was assessed by performing HPLC quantitative analysis, the maximal 457ppm of IAA was detected from the culture filtrate after culturing in R2A broth containing 0.1% tryptophan for 24h at $35^{\circ}C$. The molecular weight of the main peak obtained by LC-mass analysis was correspondent well to 175, that of IAA. The strain AMT-54 was identified as a novel species belongs to Klebsiella mobilis by a chemotaxanomic and phylogenetic analysis. To investigate the growth promoting effect of crop, when the culture broth of K. mobilis AMT-54 was infected onto seed pot of mung bean, the adventitious root induction and root growth of mung bean were 3.3times higher than control.