• Title/Summary/Keyword: Bio-alcohol

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Optimization of Ethanol Extraction Conditions for Effective Components from Gastrodia elata Blume (천마 유효성분의 에탄올 추출조건 최적화)

  • Kim Seong-Ho;Kim In-Ho;Kang Bok-Hee;Lee Sang-Han;Lee Jin-Man
    • Food Science and Preservation
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    • v.13 no.4
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    • pp.506-512
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    • 2006
  • Gastrodia elata Blume (GEB) is a traditional herbal plant that has been used in Asian countries for centuries as an anticonvulsant analgesic, and a sedative for treating general paralysis, epilepsy, vertigo, and tetanus. This study was designed to optimize conditions for ethanol extracts or GEB by analyzing and monitoring the extraction characteristics with response surface methodology. The extract was used for analysis of the effective components of GEB. The estimated optimal conditions were 63.62% in ethanol of 5.06 mL/g in solvent per sample, and 6.25 hr in extract time. The optimal extraction conditions for $ \gamma-aminobutyric$ acid, were 45.52%, 5.67 mL/g, and 6.04 hr, while those for $\rho-hydroxybenzyl$ alcohol were 62.73%, 5.02 mL/g, and 5.95 hr. Regression equation was generated for each variables and then superimposed them, such as soluble solid, $ \gamma-aminobutyric$ acid and $\rho-hydroxybenzyl$ alcohol content thereby resulting in superimposed values of extinction conditions like $45\sim65%,\;5\sim7mL/g$ and $5\sim7$ hr, respectively.

Application of β-1,3-Glucanase from Pyrococcus furiosus for Ethanol Production using Laminarin (Pyrococcus furiosus의 β-1,3-glucanase를 처리한 laminarin 분해 산물을 이용한 바이오 에탄올의 생산)

  • Kim, Dong-Gyun;Kim, Eun-Young;Kim, Yu-Ri;Kim, Joong-Kyun;Lee, Han-Seung;Kong, In-Soo
    • Journal of Life Science
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    • v.21 no.1
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    • pp.68-73
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    • 2011
  • $\beta$-1,3-glucanase from Pyrococcus furiosus was applied for the saccharification of laminarin, which is a major oligo-saccharide component of brown algae, and the reaction mixture produced from laminarin was utilized as a substrate for alcohol fermentation using yeast. To prepare the recombinant $\beta$-1,3-glucanase, a $\beta$-1,3-glucanase gene was overexpressed in Escherichia coli and purified. Laminarin was degraded to an oligo- and mono-saccharide, such as glucose, after reaction with the purified recombinant $\beta$-1,3-glucanase, and the products after enzymatic treatment were confirmed by TLC and HPLC analysis. Decomposed laminarin after enzyme reaction was only added to the medium as a C-source for yeast alcohol production reaction. 0.3% alcohol production was detected from the cultured broth by gas chromatography after 48 hr of incubation. Further evaluation for optimal conditions of saccharification and alcohol fermentation can be suggested, as well as the possibility of using this enzymatic method to produce ethanol using laminarin.

Study of Natural Preservative System Using the Mixture of Scutellariae radix, Acacia nilotica and Citrus reticulata Extracted from Polyhydric Alcohols (다가알코올로부터 추출된 황금, 아카시아, 한라봉 추출물의 천연 방부시스템 연구)

  • Park, Sung-Min;Lee, Kyeong-Ah;Yun, Mi-Young;Kim, Young-Jae;Lee, Sang-Hwa
    • KSBB Journal
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    • v.26 no.6
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    • pp.533-537
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    • 2011
  • The aim of this study was to develop a new natural preservative system to improve the weak points of natural polyhydric alcohols together with the efficiency of natural plants as a preservative. Polyhydric alcohols (glyceryl caprylate and ethylhexylglycerin) and antimicrobial plants (S. radix, A. nilotica and C. reticulata) were tested using the disc diffusion method and minimum inhibitory concentration (MIC) method for their antimicrobial activity against the common poultry pathogens, respectively. A study of the preservative efficacy of the cosmetic formulations containing the optimized preservative system demonstrated sufficient preservative efficacy against bacteria and eukaryotic test microbes. These results suggest that the natural preservative system including polyhydric alcohol extracts containing natural plants could be incorporated in cosmetic formulations.

Preparation of Superabsorbent PVA Films with Polycarboxylic Acid Crosslinkers (폴리카르복시산 가교제를 이용한 고흡수성 PVA 필름의 제조)

  • Koo, Gwang-Hoe;Yoon, Sung-Jong;Jang, Jin-Ho
    • Textile Coloration and Finishing
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    • v.21 no.4
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    • pp.39-45
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    • 2009
  • PVA films were crosslinked with dimethylol dihydroxy ethylene urea (DMDHEU) and three polycarboxylic acids of butanetetracarboxylic acid (BTCA), citric acid and malic acid Different factors influencing the crosslinking treatment with BTCA were investigated including BTCA and sodium hypophosphite (SHP) concentration, curing temperature and time. The cured films was extracted with boiling water and gel fraction was calculated based on weight change of the PVA films. The gel fraction of PVA films increased with increasing curing temperature and time. And the resistance to water and thermal stability of the crosslinked PVA films improved with the BTCA crosslinking treatment. While crosslinking with citric acid gave the highest gel fraction among the crosslinkers, crosslinking with malic acid showed the highest absorbancy in 0.9% saline solution, which was attributed to lower crosslink density and high number-average molecular weight between crosslinks. The superabsorbent PVA films could be prepared by adjusting the crosslinking condition of PVA with polycarboxilic acids.

Optimal Condition for Deacidification Fermentation of Wild Grape Wine by Mixed Culture (혼합배양에 의한 산머루주의 감산발효 최적조건)

  • Kim, Seong-Ho
    • Applied Biological Chemistry
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    • v.51 no.1
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    • pp.17-23
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    • 2008
  • In order to prevent wine quality deterioration caused by strong sour taste from raw and other materials during fermentation of wild grape wine, the various mixed cultures conditions of the deacidification fermentation and the alcohol fermentation process by inoculation of mixed strains were investigated. As a result of mixed cultures process after the inoculation of Schizosaccharomyces pombe and Schizosaccharomyces japonicus with each deacidification fermentation strain in a culture of Saccharomyces sp. SMR-3 which was used in the alcohol fermentation strain of wild grape, cultures for 12 days at $22^{\circ}C$ with Saccharomyces sp. SMR-3 and Schizosaccharomyces pombe resulted in the maximum alcohol content at $15.8{\pm}0.2%$ and the minimum with the acidity of $0.44{\pm}0.02%$, the total organic acid of $648.96{\pm}7.14$ mg% and malic acid of $99.30{\pm}1.24$ mg%. Mixed cultures with Saccharomyces sp. SMR-3 and Schizosaccharomyces pombe under the optimal condition for the deacidification fermentation of wild grape wine showed 2% higher alcohol content, 51.65% lower acidity, 48.02% lower total organic acid, and 81.12% lower malic acid than a single culture of Saccharomyces sp. SMR-3.

A Study on the Bio-Based Polyurethane (바이오 폴리우레탄에 관한 연구)

  • Ko, Jong-Sung;Lee, Jin-Hui;Sung, Ki-Chun
    • Journal of the Korean Applied Science and Technology
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    • v.29 no.3
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    • pp.531-542
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    • 2012
  • The thesis covers the trend of research on bio-based polyurethane which is made from polyols derived mainly from plant oils and isocyanates. Castor oil is a triglyceride of ricinoleic acid containing hydroxyl group. Hydroxylation is done on the unsaturated bonds of the oils by the reactions of epoxidation/ring opening, hydroformylation/hydrogenation, ozonolysis/hydrogenation, and thiol-ene reaction. Polyols from hyperbranch, primary alcohol, polysaccharide have been studied to control the reactivity of the polyol and morphology of the microdomains. Besides, researches cover biodegradable polylactic acid polyol for medical use, fatty acid dimer polyol for the prevention of hydrolysis, and polyol with ionic group for water-borne polyurethane. Bio-based polyurethanes are being used in flexible and rigid foams, coatings, sealants, and elastomers.

The Status of Production and Usage of Bio-Jet Fuel (바이오항공유 생산 및 사용현황)

  • Young-Kwan, Lim;Jin-Woo Doe
    • Applied Chemistry for Engineering
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    • v.34 no.5
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    • pp.472-478
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    • 2023
  • The usage of jet fuel has been increasing with increasing passenger and logistics movements under globalization. CO2, which is the main global warming gas from aircraft, was charged about 3.5% of total global CO2 emissions and 12% of transportation fuel emissions. For these reasons, a lot of governments and the international civil aviation organization (ICAO) are trying to reduce CO2 emissions via the introduction of bio-jet fuel. In this paper, we showed the jet fuel properties, specifications, and presentative production methods of bio-jet fuel such as alcohol to jet (ATJ), oil to jet (OTJ), gas to jet (GTJ) and sugar to jet (STJ). Also, we described the status of global and domestic bio-jet fuel usage and the policy plan for efficient distribution.

Biological Activities and Bioactive Compounds in the Extract of Acer tegmentosum Maxim. Stem (산겨릅나무 줄기추출물의 생리활성 및 유효성분 분리)

  • Hong, Bo-Kyong;Eom, Seok-Hyun;Lee, Chan-Ok;Lee, Ji-Won;Jeong, Jong-Hyun;Kim, Jae-Kwang;Cho, Dong-Ha;Yu, Chang-Yeon;Kwon, Yong-Soo;Kim, Myong-Jo
    • Korean Journal of Medicinal Crop Science
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    • v.15 no.4
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    • pp.296-303
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    • 2007
  • Acer tegmentosum (Acereaceae) has been used a source of traditional medicines for the treatment of hepatic disorders in Korea. This research was conducted to determine biofunctional activities of A. tegmentosum stem extract and to identify its bioactive components. Methanolic extract from A. tegmentosum stem was partitioned by using organic solvents, including n-hexane, ethyl acetate, n-butanol, and water. Two compounds were isolated by using an ODS column chromatography from ethyl acetate soluble fraction shown to the strongest antioxidant activity ($RC_{50}=3.15\;{\mu}g/m{\ell}$) among the fractions. The isolated compounds were analyzed by $^1H$ and $^{13}C$ NMR, IR, UV/VIS, MS spectrum data and identified as catechin, ${\rho}-Hydroxyphenethyl$ alcohol $1-O-{\beta}-_D-(6'-O-galloyl)-glucopyranoside$. The compounds have shown strong antioxidant activity, with similar activity to BHA ($RC_{50}=2\;{\mu}g/m{\ell}$). Especially, ${\rho}-Hydroxyphenethyl$ alcohol 1-O-{\beta}-_D-(6'-O-galloyl)-glucopyranoside$ was shown strong anti-lipid peroxidative activity. However, the compounds were not shown antimicrobial activities. In antimicrobial activity assays, ethyl acetate soluble fraction was effective to bacterial inhibition, such as Escherichia coli and Klebsiella pneumonia, with minimum inhibitory concentrations in $125\;{\mu}g/m{\ell}$. Otherwise, antifungal activity against Candida albicans was shown in n-hexane soluble fraction exhibiting $63\;{\mu}g/m{\ell}$ of minimum inhibitory concentration. In anticomplementary activity assays, water soluble fraction was the most effective exhibiting 24% inhibitory activity.

Study on Conversion of Carbon Dioxide to Methyl Alcohol over Ceramic Monolith Supported CuO and ZnO Catalysts (세라믹 모노리스에 담지된 CuO와 ZnO계 촉매에 의한 이산화탄소의 메탄올로의 전환에 관한 연구)

  • Park, Chul-Min;Ahn, Won-Ju;Jo, Woong-Kyu;Song, Jin-Hun;Kim, Ki-Joong;Jeong, Woon-Jo;Sohn, Bo-Kyun;Ahn, Byeong Kwon;Chung, Min-Chul;Park, Kwon-Pil;Ahn, Ho-Geun
    • Journal of Korean Society for Atmospheric Environment
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    • v.29 no.1
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    • pp.97-104
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    • 2013
  • Methyl alcohol is one of the basic intermediates in the chemical industry and is also being used as a fuel additive and as a clean burning fuel. In this study, conversion of carbon dioxide to methyl alcohol was investigated using catalytic chemical methods. Ceramic monoliths (M) with $400cell/in^2$ were used as catalyst supports. Monolith-supported CuO-ZnO catalysts were prepared by wash-coat method. The prepared catalysts were characterized by using ICP analysis, TEM images and XRD patterns. The catalytic activity for carbon dioxide hydrogenation to methyl alcohol was investigated using a flow-type reactor under various reaction temperature, pressure and contact time. In the preparation of monolith-supported CuO-ZnO catalysts by wash-coat method, proper concentration of precursors solution was 25.7% (w/v). The mixed crystal of CuO and ZnO was well supported on monolith. And it was known that more CuO component may be supported than ZnO component. Conversion of carbon dioxide was increased with increasing reaction temperature, but methyl alcohol selectivity was decreased. Optimum reaction temperature was about $250^{\circ}C$ under 20 atm because of the reverse water gas shift reaction. Maximum yield of methyl alcohol over CuO-ZnO/M catalyst was 5.1 mol% at $250^{\circ}C$ and 20 atm.