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Optimization of Ethanol Extraction Conditions for Effective Components from Gastrodia elata Blume  

Kim Seong-Ho (Department of Herbs & Food Science, Kyungbook College of Science)
Kim In-Ho (Bio-Health Industrial Institute, Kyungbook College of Science)
Kang Bok-Hee (Bio-Health Industrial Institute, Kyungbook College of Science)
Lee Sang-Han (Department of Food Science & Technology, Kyungpook National University)
Lee Jin-Man (Department of Herbs & Food Science, Kyungbook College of Science)
Publication Information
Food Science and Preservation / v.13, no.4, 2006 , pp. 506-512 More about this Journal
Abstract
Gastrodia elata Blume (GEB) is a traditional herbal plant that has been used in Asian countries for centuries as an anticonvulsant analgesic, and a sedative for treating general paralysis, epilepsy, vertigo, and tetanus. This study was designed to optimize conditions for ethanol extracts or GEB by analyzing and monitoring the extraction characteristics with response surface methodology. The extract was used for analysis of the effective components of GEB. The estimated optimal conditions were 63.62% in ethanol of 5.06 mL/g in solvent per sample, and 6.25 hr in extract time. The optimal extraction conditions for $ \gamma-aminobutyric$ acid, were 45.52%, 5.67 mL/g, and 6.04 hr, while those for $\rho-hydroxybenzyl$ alcohol were 62.73%, 5.02 mL/g, and 5.95 hr. Regression equation was generated for each variables and then superimposed them, such as soluble solid, $ \gamma-aminobutyric$ acid and $\rho-hydroxybenzyl$ alcohol content thereby resulting in superimposed values of extinction conditions like $45\sim65%,\;5\sim7mL/g$ and $5\sim7$ hr, respectively.
Keywords
Gastrodia elata Blume; extraction condition; effective component; response surface methodology;
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1 Lin, J.H., Liu, Y.C., Hau, J.P. and Wen, K.C. (1996) Parishins B and C from rhizomes of Gastrodia elata. Phytochem., 42, 549-551   DOI   ScienceOn
2 Lee, Y.M. (1990) In Oriental Medicine Dictionary, Sammundang, Seoul. p.814
3 Sin, K.G. (1980) In Sinssi Herbology, Sumunsa, Korea. p.288-290
4 Paik, Y.S., Song, J.K., Yoon, C.H., Chung, K.S. and Yun-Choi, H.S. (1995) Anti-platelet and anti-thrombotic effects of Gastrodia elata. Kor. J. Pharmacogn., 26, 385-389
5 Yun-Choi, H.S., Pyo, M.K. and Park, K.M. (1998) Isolation of 3-O-(4' -hydroxy-benzyl)- $\beta$-sitosterol and 4[4' (4'-hydroxybenzyloxy)benzyloxy] benzyl methyl ether from fresh tubers of Gastrodia elata. Arch. Pharm. Res., 21, 357-360   DOI   ScienceOn
6 Yun-Choi, H.S. and Pyo, M.K. (1997) Isolation of 4,4' -dihydroxybenzyl sulfoxide from Gastrodia elata. Arch. Pharm. Res., 20, 91-92   DOI   ScienceOn
7 Martha, L.A. and James, P.B. (1992) The mathematic a handbook, compatible with mathematica version 2.0, An inprint of academic press, Inc. Harcourt Brace & Co., Massachusetts, p.15- 511
8 SAS institute, Inc. (1988) In SAS/STAR User's Guide, Version 6. Cary, NC, USA
9 Ku, B.H. (1991) Experimental studies on the pharmaceutical effects of Gastrodiae elata, M.S. thesis, KyungHee University, Seoul, Korea
10 Shin, C.S., Park, C.K., Lee, J. W., Lee, J.G., Jang, C.K. and Kim, Y.K. (1999) Analysis of the components with freeze drying and steam drying of Gastrodia elata Blume. J. Kor. Soc. Food Sci. Nutr., 28, 1058-1063   과학기술학회마을
11 Kim, E.J., Ji, G.E. and Kang, Y.H. (1994) Effects of Gastrodia Rhezoma extracts on global coronary circulation in rats. Kor. J. Food Sci. Technol., 26, 213-220
12 Kim, C.M., Sin, M.K, An, D.K. and Lee, K.S. (1997) In Zoungyank Dictionary, Jungdam, Korea. p.4105-4110
13 Wanasundara, P.K.J.P.D. and Shahidi, F. (1996) Optimization of hexametaphosphate-assisted extraction of flaxseed proteins using response surface methodology. Kor. J. Food Sci., 6, 604-607
14 Huh. K., Kim, J.S., Kwon, T.H., Kim, J.A., Yoag, C.S., Ha, J.H. and Lee, D.U. (1998) The mechanism of anticonvulsive effect of the rhizoma of Gastrodia elata in pentylenetetrazole treated rats. J. Kor. Pharm. Sci., 42, 330-335
15 Ha, J.H., Lee, D.U., Eah, K.Y., Ha, J.S., Kim,.H.J., Yoag, C.S. and Huh, K. (1997) Modulation of ligand binding to the GABA-benzodiazepine receptor complex by Gastrodia elata Blmne. J. of Appl. Pharmacol., 5, 325-330   과학기술학회마을
16 Oh, S.L., Kim, S.S., Min, B.Y. and Chung, D.H. (1990) Composition of free sugars, free amino acids, non-volatile organic acids and tannins in the extracts of L chinensis., A. acutiloba K, S. chinensis B. and A. sessiliflorum S. Korean J. Food Sci. Technol., 22, 76-81
17 Yun-Choi, H.S., Pyo, M.K. and Park, K.M. (1997) Circiumaldehyde from Gastrodia elata. Nat. Prod. Sci., 3, 104-105
18 Kwon, J.H., Belanger, J.M.F. and Pare, J.R.J. (2003) Optimization of microwave-assited extract(MAP) for ginseng components by response surface methodology. J. Agric. Food Chem., 51, 1807-1810   DOI   ScienceOn
19 Taguchi, H., Yosioka, I., Yamasaki, K. and Kim, I.L. (1996) Studies on the constituents of Gastrodia elata Blume. Chem. Pharm. Bull., 29, 55-62
20 Chung, H.S. and Ji, G.E. (1996) Composition and functionality of Chonma. Kor. J. Food Sci. Technol., 28, 53-57
21 Shaz, W.S. (1985) HPLC determination of Gastrodia and 4-hydroxybenzyl alcohol in Gastrodia ealta. Yaown Fenxi Zashi, 5, 218-221