• Title/Summary/Keyword: Bio-Oil

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Biosynthesis of Polyunsaturated Fatty Acids: Metabolic Engineering in Plants (고도불포화지방산 생합성: 식물에서의 대사공학적 응용)

  • Kim, Sun-Hee;Kim, So-Yun;Kim, Jong-Bum;Roh, Kyung-Hee;Kim, Young-Mi;Park, Jong-Sug
    • Journal of Applied Biological Chemistry
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    • v.52 no.3
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    • pp.93-102
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    • 2009
  • Polyunsaturated fatty acids (PUFA), especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have significantly beneficial effects on health in relation to cardiovascular, immune, and inflammatory conditions and they are involved in determining the biophysical properties of membranes as well as being precursors for signaling molecules. PUFA biosynthesis is catalyzed by sequential desaturation and fatty acyl elongation reactions. This aerobic biosynthetic pathway was thought to be taxonomically conserved, but an alternative anaerobic pathway for the biosynthesis of PUFA is now known to contain analogous polyketide synthases (PKS). Certain fish oil can be a rich source of PUFA although processed marine oil is generally undesirable as food ingredients because of the associated objectionable flavors that are difficult and cost-prohibitive to remove. Oil-seed plants contain only the 18-carbon polyunsaturated fatty acid alpha-linolenic acid, which is not converted in the human body to EPA and DHA. It is now possible to engineer common oilseeds which can produce EPA and DHA and this has been the focus of a number of academic and industrial research groups. Recent advances and future prospects in the production of EPA and DHA in oilseed crops are discussed here.

The Color Change of Korean Pine Specimens Oil-Heat-Treated at 180 and 200℃ (180℃와 200℃ 오일열처리 잣나무재의 재색변화)

  • Lee, Won-Hee;Lim, Ho-Mook;Kang, Ho-Yang
    • Journal of the Korean Wood Science and Technology
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    • v.43 no.4
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    • pp.438-445
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    • 2015
  • Among several thermal wood modification methods German oil heating technology was applied to Korean pine specimens by heat treatment at $180^{\circ}C$ and $200^{\circ}C$. In addition the matched specimens were heat-treated by Thermowood method. The heat-treated specimens were planed and their colors were measured with a colorimeter at 1 mm and 4 mm below the surfaces. The average lightness index (L*) of Oil-180 specimens was the largest followed by Oil-200 and Tmo-200 specimens. The average redness indexes (a*) of all specimens were increased by heat treatment, while the average yellowness indexes (b*) were variable. The average color differences (${\Delta}E*$) of Oil-200 and Tmo-200 specimens were above 30.0, while that of Oil-180 specimens was only 18.4. It can be concluded that the colors of the heat-teated specimens were uniformly changed because their average color difference indexes (${\Delta}E*$) between the inner layers were below 6.0.

Gossypii Semen oil alleviates memory dysfunction in scopolamine-treated mice (면화자 정유의 기억력 손상 완화 효과)

  • Lee, Jihye;Jung, Eun Mi;Lee, Eunhong;Jang, Gwi Yeong;Seo, Kyung Hye;Kim, Mi Ryeo;Jung, Ji Wook
    • The Korea Journal of Herbology
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    • v.36 no.2
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    • pp.1-9
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    • 2021
  • Objectives : Gossypium arboreum (cotton) is traditionally used to treat various health disorders. However, anti-amnesic effect of G. arboreum has not been reported. The objective of this study was to investigate in-vivo the anti-amnesic effects along with in vitro antioxidant and acetylcholinesterase (AChE) inhibition potential in G. arboreum seed essential oil. Methods : The essential oil of G. arboreum obtained by solid phase microextraction (SPME) techniques were identified by gas chromatography-mass spectroscopy (GC-MS). 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay were performed to determine the antioxidant activity at various concentrations (312.5, 625, 1250, 2500, 5000, 10000 ㎍/㎖. Y-maze, passive avoidance and Morris water maze tests were carried out to evaluate improved effect on scopolamine (1 mg/kg)-induced memory dysfunction at the dose level of 50, 100 and 200 mg/kg. Donepezil (5 mg/kg) was used as a positive drug control. We performed acetylcholinesterase (AChE) activity assay in ex vivo. Results : Five volatile compounds were identified in G. arboreum. The assays of DPPH and ABTS revealed that G. arboreum increased antioxidant activity in a dose-dependent manner. G. arboreum ameliorated the percent of spontaneous alternation in the Y-maze test, shortened step-through latency in the passive avoidance test, and increased swimming time in the target zone in the Morris water maze test. In addition, G. arboreum inhibited the AChE activity. Conclusions : Based on these findings, G. arboreum may aid in the prevention and treatment of learning and memory-deficit disorders through antioxidant and AChE inhibitory activities.

Dispersing Properties of Heavy Crude Oil according to Dispersant Structures (중유용 분산제 구조에 따른 중유 분산 특성)

  • Son, Jeong-Mae;Kim, Nam-Kyun;Shin, Jihoon;Yang, Youngdo;Kim, Young-Wun
    • Tribology and Lubricants
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    • v.31 no.6
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    • pp.251-257
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    • 2015
  • Heavy oil production is receiving significant attention because of increased demands for thermal power generation systems of the diesel engine and boilers. However, asphaltene, which is a heavy oil components (6-8 wt%), reduces the heat efficiency of the fuels owing to its agglomerated sludge of asphaltene during the burning process. Therefore, for hassle-free operation, we should develop asphaltene dispersants to suppress the formation of the sludge. We prepare variable salt-type polymeric dispersants using poly(isobutenyl succinic anhydride) and poly(amine) through both condensation esterification and acid-base neutralization reactions, which we subsequently evaluate for dispersing performance, using Turbiscan measurement. Total acid number (TAN) and total base number (TBN) of 75Lec-25SynDis.2 composed of lecithin and the prepared polymeric salt having the ratio of 3 : 1 are 18.9 and 33.7 mg KOH/g, respectively, which are comparable to those of the commercial dispersants (15.8 and 26.5 mg KOH/g). We determine the initial turbidity observed for 15 min of the polymeric dispersant was determined with transmittance (%), which can be calculated to separability number (SN). The SN value of 75Lec-25SynDis.2 is close to zero, which is superior to that of commercial dispersants and lecithin (0.015 and 0.017).

A Cytotoxicity of Carrier Oil and Essential Oils on Cells by Using of Aromatherapy (향기요법에 사용하는 캐리어 오일과 에션셜 오일의 세포에 대한 독성)

  • Yu, Byong-Soo;Kim, Sha-Sha;Yun, Young-Han;Kim, Ki-Young
    • Korean Journal of Human Ecology
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    • v.17 no.5
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    • pp.1027-1035
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    • 2008
  • Essentail oils and carrier oils are generally used for Aromatherapy. Therefore the toxicity, possibilities of irritations and sensitive reactions and injury of essential oils must be considered for clients and therapists. So that, in this studies a toxicity of jojoba and 4 species essential oils (fennel, mandarine, tea tree and cedarwood) were investigated by the measurement of MTT-assay and sirius red staining. Liver, kidney and brain tell were chosen for the cell viability assay and observation of morphological change. In the result, no cytotoxicity was observed on live., kidney and brain cell at concentration of 0.01 $\mu\el/m\el$ jojoba oil. And lysis and nucleus breaking were not observed at same concentration of jojoba oil on live., kidney and brain cell. fennel oil was showed 50% of cell viability and inhibited cell growth on liver, kidney and brain cell at relatively high concentration compared with the other oils. 50% of liver, kidney and brain cell viability and delayed cell growth of tea tree and mandarine oil were revealed at lower concentration than fennel oil. In cedarwood oil, 50% of liver cell viability at concentration of 0.00067 $\mu\el/m\el$ was showed, but cell viability and cell growth of kidney and beam cell were effected at the lowest concentration compared with other oils. So that, jojoba oil as using of carrier oil may be not harmful. And 3 essential oils from the fennel, tea tree and mamdarine may have very low toxicity, but cedarwood may be used carefully for inhalation. And over dosage of concentrated cedarwood oil should be not directly touched and exposured, and absolute essential oils must be diluted with carrier oils for topical and systematic massage.

Enhanced Essential Oil Formation by Two-phase Culture of Mentha piperita Cells in Shake Flask and Air-lift Bioreactors

  • Kim, Teresa;Kim, Tae-Yong;Bae, Geun-Won;Chae, Young-Am;Lee, Hyong-Joo;Chung, In-Sik
    • Journal of Microbiology and Biotechnology
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    • v.5 no.6
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    • pp.364-369
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    • 1995
  • Effect of two-phase culture on Mentha piperita cell growth and essential oil formation was investigated using shake flask and air-lift bioreactors. LiChroprep RP-B(RP-B) addition did not impair M. piperita cell growth, but resulted in stimulated formation of essential oils and increased ratios of extracellular oil to intracellular oil formation. However, the combined use of RP-B and chitosan elicitor was not synergistic. Volumetric productivity of essential oils in RP-B treated culture using cell-recycled air-lift bioreactor was $6.9\;\mu\textrm{g}/l{\cdot}day$ which was substantially higher than that obtainable from the control. Our results demonstrate the potential of a second phase to enhance overall productivity for M. piperita cell culture.

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Performance and Emission Studies in a DI Diesel Engine Fuelled with Diesel-Pyrolysis Oil Emulsion (디젤-열분해유 유상액을 사용하는 직접분사식 디젤 엔진의 엔진성능 및 배기특성에 관한 연구)

  • Lee, Seokhwan;Kim, Hoseung;Kim, Taeyoung;Woo, Sejong;Kang, Kernyong
    • Journal of ILASS-Korea
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    • v.19 no.2
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    • pp.55-63
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    • 2014
  • Pyrolysis oil (PO), also known as Bio crude oil (BCO), has the potential to displace significant amounts of fuels that are currently derived from petroleum sources. PO has been regarded as an alternative fuel for petroleum fuels to be used in diesel engine. However, the use of PO in a diesel engine requires modifications due to low energy density, high water contents, low acidity, and high viscosity of the PO. One of the easiest way to adopt PO to diesel engine without modifications is emulsification of PO with the fuels that has higher cetane number. However, PO that has high amount of polar chemicals is immiscible with non polar hydrocarbons of diesel. Thus, to stabilize a homogeneous phase of diesel-PO blends, a proper surfactant should be used. In this study, a DI diesel engine operated with diesel and diesel-PO emulsions was experimentally investigated. Performance and gaseous & particle emission characteristics of a diesel engine fuelled by diesel-PO emulsions were examined. Results showed that stable engine operation was possible with the emulsions and engine output power was comparable to diesel operation.

Improvement of the Quality of Dried Wild Vegetables by Micro Oil Sprayed Thermal Air Technique

  • Yonghyun Kim;Yunmi Park;Chul-Woo Kim;Uk Lee;Hyun Ji Eo
    • Korean Journal of Plant Resources
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    • v.35 no.6
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    • pp.778-784
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    • 2022
  • Wild vegetables, such as Cirsium setidens, and Aster scaber, are commonly distributed as dried materials because the wild vegetables lose their freshness quickly after harvest and distribution. Dried wild vegetables require rehydration to use as a food ingredient, and the quality of rehydrated wild vegetables is affected by pre-drying and drying methods. Here, we introduce the newly developed pre-drying and drying method, termed "micro oil sprayed thermal air (MOTA) technique". The three wild vegetables processed by the MOTA technique showed improved rehydration rate and reduced time to achieve maximum rehydration rate. Color characteristics were also improved in C. setidens. These results indicate that the MOTA technique improves the overall quality of rehydrated wild vegetables. It is expected that our findings could enhance the marketability of dried wild vegetables by improving overall quality and reducing preparation time.

Selective Production of Aromatic Aldehydes from Heavy Fraction of Bio-oil via Catalytic Oxidation

  • Li, Yan;Chang, Jie;Ouyang, Yong;Zheng, Xianwei
    • Bulletin of the Korean Chemical Society
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    • v.35 no.6
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    • pp.1654-1658
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    • 2014
  • High value-added aromatic aldehydes (e.g. vanillin and syringaldehyde) were produced from heavy fraction of bio-oil (HFBO) via catalytic oxidation. The concept is based on the use of metalloporphyin as catalyst and hydrogen peroxide ($H_2O_2$) as oxidant under alkaline condition. The biomimetic catalyst cobalt(II)-sulfonated tetraphenylporphyrin ($Co(TPPS_4)$) was prepared and characterized. It exhibited relative high activity in the catalytic oxidation of HFBO. 4.57 wt % vanillin and 1.58 wt % syringaldehyde were obtained from catalytic oxidation of HFBO, compared to 2.6 wt % vanillin and 0.86 wt % syringaldehyde without $Co(TPPS_4)$. Moreover, a possible mechanism of HFBO oxidation using $Co(TPPS_4)/H_2O_2$ was proposed by the research of model compounds. The results showed that this is a promising and environmentally friendly method for production of aromatic aldehydes from HFBO under $Co(TPPS_4)/H_2O_2$ system.

Effect of the Recycling of Non-condensable Gases on the Process of Fast Pyrolysis for Palm Wastes (미응축가스 재순환에 따른 팜 부산물 급속열분해 반응 공정 특성)

  • Oh, Changho;Lee, Jang Hoon
    • Clean Technology
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    • v.24 no.3
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    • pp.233-238
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    • 2018
  • Bio-oil is produced by the fast quenching of hot vapor produced by fast pyrolysis of biomass in an inert atmosphere. Nitrogen is used as carrier gas to control the concentration of oxygen less than 3%. The consumption of nitrogen should be increased with increasing process size, and leading to increasing of facility and operating costs due to nitrogen charge. The effects of the recycling of non-condensable gases on the fast pyrolysis, bio-oil yield and quality, and nitrogen consumption have systematically investigated to see the possibility of these results in fast pyrolysis process of palm residue.