• 한국생물공학회:학술대회논문집
• /
• 2003.10a
• /
• pp.123-123
• /
• 2003

• 한국생물공학회:학술대회논문집
• /
• 2002.04a
• /
• pp.365-368
• /
• 2002

• Bulletin of the Korean Chemical Society
• /
• v.32 no.spc8
• /
• pp.3152-3154
• /
• 2011

• 한국생물공학회:학술대회논문집
• /
• 2005.04a
• /
• pp.430-430
• /
• 2005

• 한국생물공학회:학술대회논문집
• /
• 2005.10a
• /
• pp.584-584
• /
• 2005

• 한국생물공학회:학술대회논문집
• /
• 2005.10a
• /
• pp.585-585
• /
• 2005

• 한국생물공학회:학술대회논문집
• /
• 2003.10a
• /
• pp.149-150
• /
• 2003

• 한국생물공학회:학술대회논문집
• /
• 2005.10a
• /
• pp.757-758
• /
• 2005

• Tissue Engineering and Regenerative Medicine
• /
• v.15 no.6
• /
• pp.761-769
• /
• 2018

BACKGROUND: Bioprinting has recently appeared as a powerful tool for building complex tissue and organ structures. However, the application of bioprinting to regenerative medicine has limitations, due to the restricted choices of bio-ink for cytocompatible cell encapsulation and the integrity of the fabricated structures. METHODS: In this study, we developed hybrid bio-inks based on acrylated hyaluronic acid (HA) for immobilizing bio-active peptides and tyramine-conjugated hyaluronic acids for fast gelation. RESULTS: Conventional acrylated HA-based hydrogels have a gelation time of more than 30 min, whereas hybrid bio-ink has been rapidly gelated within 200 s. Fibroblast cells cultured in this hybrid bio-ink up to 7 days showed >90% viability. As a guidance cue for stem cell differentiation, we immobilized four different bio-active peptides: BMP-7-derived peptides (BMP-7D) and osteopontin for osteogenesis, and substance-P (SP) and Ac-SDKP (SDKP) for angiogenesis. Mesenchymal stem cells cultured in these hybrid bio-inks showed the highest angiogenic and osteogenic activity cultured in bio-ink immobilized with a SP or BMP-7D peptide. This bio-ink was loaded in a three-dimensional (3D) bioprinting device showing reproducible printing features. CONCLUSION: We have developed bio-inks that combine biochemical and mechanical cues. Biochemical cues were able to regulate differentiation of cells, and mechanical cues enabled printing structuring. This multi-functional bio-ink can be used for complex tissue engineering and regenerative medicine.

• Proceedings of the Korean Society for Bio-Environment Control Conference
• /
• 2003.04a
• /
• pp.64-69
• /
• 2003

노동 집약적이던 농업은 급속한 기계화로 인하여 노동력의 절감은 여러 분야에서 활성화되고 있지만, 농업은 수익성이 높지 않아 여전히 사람들이 노동을 기피하는 산업중의 하나이다. 온실 자동화는 특용 작물 등의 고부가가치 상품에만 한정되어 있으므로 이를 개선해야 한다. (중략)