• 환경위생공학
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• 제22권1호
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• pp.1-16
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• 2007

The incineration process has commonly used for wastes amount reduction and thermal treatments of pollutants as the technologies accumulated. However, the process is getting negative public images owing to matter of hazardous pollutants emission. Specially dioxins became a main issue and is mostly emitted from municipal solid wastes incineration. In this reason, pyrolysis/gasfication/melting process is presented as a alternative of incineration process. The pyrolysis/gasfication/melting process, a novel technology, is middle of verification of commercial plant and development of technologies in Korea. But the survey about the pollutant emission from the process, and background data in these facilities is necessary. So in this survey, it Is investigated that the behavior of dioxins in three pyrolysis/gasfication/melting plant (S, T, P) of pilot scale. In case of S plant, concentration of dioxins shows high at latter part of cogenerated boiler and stack which are operate on low temperature conditions than a latter parts of pyrolysis and melting furnace which are operate on high temperature condition. Concentration of gas phage dioxins had increased after combusted gas passed cogenerated boiler and this is attributed to react of precursor materials such as chlorobenzene and chlorophenol. Concentration of dioxins in T plant showed lower levels at latter part of cooling equipment which are operate with water spray type on low temperature conditions than a latter parts of gasfied melting furnace which are operate on high temperature condition. Removal efficiency of dioxins at gas treatment equipment was 78.8 %. Concentration of dioxins in P plant was low at latter part of SDA/BF which is operate at low temperature conditions than a latter parts of pyrolysis gasfied chamber which are operate at high temperature condition. Removal efficiency of dioxins of SDA/BF was 85.9 % and therefore, it showed high efficiency at those of stoker type incineration facility. However, concentration of dioxins which emitted at high temperature condition were low in three facilities and satisfied present standard emission level of dioxins. To consider the distribution ratio of dioxins, Particulate phase dioxins at S and P plants showed similar ratio with which shows in current stoker type for middle scale domestic waste incineration facility. It is necessary to continuos monitoring the ratio of distribution of dioxins in T plant in because ratio of gas phage dioxins showed high.

• Journal of Ginseng Research
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• 제44권6호
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• pp.799-807
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• 2020

Background: The skin acts as a barrier to protect organisms against harmful exogenous agents. Compound K (CK) is an active metabolite of ginsenoside Rb1, Rb2 and Rc, and researchers have focused on its skin protective efficacy. In this study, we hypothesized that increased expression of the serine protease inhibitor Kazal type-5 (SPINK5) may improve skin barrier function. Methods: We screened several ginsenosides to increase SPINK5 gene promoter activity using a transactivation assay and found that CK can increase SPINK5 expression. To investigate the protective effect of CK on the skin barrier, RT-PCR and Western blotting were performed to investigate the expression levels of SPINK5, kallikrein 5 (KLK5), KLK7 and PAR2 in UVB-irradiated HaCaT cells. Measurement of transepidermal water loss (TEWL) and histological changes associated with the skin barrier were performed in a UVB-irradiated mouse model and a 1-chloro-2,4-dinitrobenzene (DNCB)-induced atopic dermatitis-like model. Results: CK treatment increased the expression of SPINK5 and decreased the expression of its downstream genes, such as KLKs and PAR2. In the UVB-irradiated mouse model and the DNCB-induced atopic dermatitis model, CK restored increased TEWL and decreased hydration and epidermal hyperplasia. In addition, CK normalized the reduced SPINK5 expression caused by UVB or DNCB, thereby restoring the expression of the proteins involved in desquamation to a level similar to normal. Conclusions: Our data showed that CK contributes to improving skin-barrier function in UVB-irradiated and DNCB-induced atopic dermatitis-like models through SPINK5. These results suggest that therapeutic attempts with CK might be useful in treating barrier-disrupted diseases.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제35권1호
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• pp.38-50
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• 2013

Purpose: The aim of this study is to evaluate the effect and potential of electron beam (E-beam) irradiation treatment to the synthetic bony mixtures composed of hydroxyapatite (HA; Bongros$^{(R)}$, Bio@ Co., Korea) and tricalcium phosphate (${\beta}$-TCP, Sigma-Aldrich Co., USA), mixed at various ratios and of type I collagen (Rat tail, BD Biosciences Co., Sweden) as an organic matrix. Methods: We used 1.0~2.0 MeV linear accelerator and 2.0 MeV superconductive linear accelerator (power 100 KW, pressure 115 kPa, temperature $-30{\sim}120^{\circ}C$, sensor sensitivity 0.1~1.2 mV/kPa, generating power sensitivity 44.75 mV/kPa, supply voltage $5{\pm}0.25$ V) with different irradiation dose, such as 1, 30 and 60 kGy. Structural changes in this synthetic bone material were studied in vitro, by scanning electron microscopy (SEM), elementary analysis and field emission scanning electron microscope (FE-SEM), attenuated total reflection (ATR), and electron spectroscopy for chemical analysis (ESCA). Results: The large particular size of HA was changed after E-beam irradiation, to which small particle of TCP was engaged with organic collagen components in SEM findings. Conclusion: The important new in vitro data to be applicable as the substitutes of artificial bone materials in dental and medical fields will be able to be summarized.

• 농촌지도와개발
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• 제20권3호
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• pp.643-678
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• 2013

본 연구의 목적은 5 16 군정기 지방행정의 많은 환경변화 속에서 각 도가 추진한 '모범부락조성사업'을 통해 초기 농촌마을개발사업에 대한 모습, 특히 지방행정이 사업의 주체가 되기 위해 추진한 방법을 검토하여 시사점을 찾는데 있다. 군정기(1961~1963) 지방행정은 시군을 기초자치단체로 하는 행정체계 개편, 국가기획제도 도입, 지방세제 개편, 새로운 농촌기구 설립으로 인한 농촌지도 다원화 등의 여러 변화요인을 맞게 되었다. 이러한 환경 속에서 지역의 개발행정 주축은 지방자치단체로 옮겨져 갔고, 각 도는 '보고가는 마을(전북)', '빛나는 마을(충남)'등의 각종 명칭으로 모범부락조성사업을 추진하였다. 또한 내무부는 지역개발의 장기적인 종합계획으로서 군 건설계획의 작성과 추진을 지시하였다. 본 연구는 국가 정책을 중심으로 한 농촌지역개발사에서 도 단위의 지방사를 발굴하고 이를 지방행정 환경변화와 연계하여 분석하였다는 측면에서 향후 관련 연구의 기초자료로 활용될 수 있으리라 판단된다.

• Genomics & Informatics
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• 제16권2호
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• pp.22-29
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• 2018

Incorporation of unique barcodes into fission yeast gene deletion collections has enabled the identification of gene functions by growth fitness analysis. For fine tuning, it is important to examine barcode sequences, because mutations arise during strain construction. Out of 8,708 barcodes (4,354 strains) covering 88.5% of all 4,919 open reading frames, 7,734 barcodes (88.8%) were validated as high-fidelity to be inserted at the correct positions by Sanger sequencing. Sequence examination of the 7,734 high-fidelity barcodes revealed that 1,039 barcodes (13.4%) deviated from the original design. In total, 1,284 mutations (mutation rate of 16.6%) exist within the 1,039 mutated barcodes, which is comparable to budding yeast (18%). When the type of mutation was considered, substitutions accounted for 845 mutations (10.9%), deletions accounted for 319 mutations (4.1%), and insertions accounted for 121 mutations (1.6%). Peculiarly, the frequency of substitutions (67.6%) was unexpectedly higher than in budding yeast (~28%) and well above the predicted error of Sanger sequencing (~2%), which might have arisen during the solid-phase oligonucleotide synthesis and PCR amplification of the barcodes during strain construction. When the mutation rate was analyzed by position within 20-mer barcodes using the 1,284 mutations from the 7,734 sequenced barcodes, there was no significant difference between up-tags and down-tags at a given position. The mutation frequency at a given position was similar at most positions, ranging from 0.4% (32/7,734) to 1.1% (82/7,734), except at position 1, which was highest (3.1%), as in budding yeast. Together, well-defined barcode sequences, combined with the next-generation sequencing platform, promise to make the fission yeast gene deletion library a powerful tool for understanding gene function.

• Journal of Korean Biological Nursing Science
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• 제5권1호
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• pp.13-22
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• 2003

The purpose of this study was to investigate and analyze current educational requirements related to genetics curriculum(from June 2002 to September 2002) established at nursing institutions and to provide the basic data for the development of genetics science program at the undergraduate. Subjects of this study were comprised of twenty-three colleges of nursing in 4-year baccalaureate and thirty colleges in 3-year diploma programs. The results of this study were as follows : 1) 32 colleges offer courses related to genetics. 29 among 32 colleges have that integrated. Three schools have established completely independent courses of genetics. 21 colleges do not have any courses dealing with genetics. 2) The contents of courses related to genetics include: Congenital abnormalities, chromosomal aberrations, congenital metabolic disease, prenatal diagnosis and genetic counseling, genes and chromosomes, immune genetics, blood type and genetics, rule of genetics, variation in gene expression, the map of the human gene, gene linkage genetics, interaction of genes, single inheritance in order and genetic biochemistry. 3) For course credit, 14colleges(48.3%) offered at most 1 credit per course. The grade of student who can take the course, 51.7% were in their second year while 37.9% were in their third year. The majors of nursing faculty who taught the course were nursing(51.7%) and basic nursing science(17.2%). 4) As far as the need of opening the courses related to genetics, 36 colleges(67.0%) have made a 'need', 12 schools(22.6%) state 'dose not need'. 711e reason for need were the following development of bio engineering, increase number of patients who are related to genetics, recognition of the need in clinical nursing. 7 schools(13.2%) agreed to offer independent course in genetics but 39 schools(73.6%) are in disagreement with that. When the school offers the course with other courses, 27 schools(50.0%) are opening basic nursing science and 14 schools(26.4%) are opening nursing as an integrated courses. If the name of course was either genetic nursing(34.0%) or genetics(28.3%), the credits for the course was one or 2 credits. 33 schools(62.3%) students were in the first or second years. 41 schools(84.9%), the majors of the faculty who had taught the course were either basic nursing science(35.8%), nursing(28.3%) or basic medicine(24.5%). The contents of the course should include in that order: Chromosome aberrations, prenatal diagnosis and genetic counseling, congenital metabolic disease, congenital abnormalities, genes and chromosomes, the rules of genetics, immune genetics, interaction of genes, variation in gene expression, etc. The results and discussions of the study indicate that the entire curriculums need to be investigated with respect to contents of education, nursing curriculums and name of courses because of the increasing need of knowledge related to genetics in the clinical practice.

• 식물병연구
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• 제22권4호
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• pp.249-256
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• 2016

국내 딸기 주산단지의 시설재배지 170개 포장으로부터 토양시료를 채취하여 식물기생선충을 조사한 결과, 농업적으로 중요한 뿌리썩이선충이 96개포장(56.5%), 뿌리혹선충이 63개 포장(37.1%)에서 검출되었다. 이 중 44개 포장은 뿌리썩이선충과 뿌리혹선충이 복합감염된 것으로 나타났다. 뿌리썩이선충과 뿌리혹선충의 도별 발생은 경남지역이 각각 68%와 46%, 시 군별로는 진주시가 81%와 54%로 가장 높았다. 국내 최대의 딸기 수출재배단지이자 식물기생선충의 검출률이 가장 높았던 진주시의 뿌리썩이선충과 뿌리혹선충의 종 동정 결과, 사과뿌리썩이선충(P. vulnus), 당근뿌리혹선충(M. hapla)이 우점하고 있는 것으로 나타났다.

• Asian Pacific Journal of Cancer Prevention
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• 제17권3호
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• pp.1129-1134
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• 2016

Vulva and Vaginal cancers are rare among all gynecological cancers worldwide, including Thailand, and typically affect women in later life. Persistent high risk human papillomavirus (HR-HPV) infection is one of several important causes of cancer development. In this study, we focused on HPV investigation and specific type distribution from Thai women with abnormality lesions and cancers of the vulva and Vaginal. A total of ninety paraffin-embedded samples of vulva and Vaginal abnormalities and cancer cells with histologically confirmed were collected from Thai women, who were diagnosed in 2003-2012 at the National Cancer Institute, Thailand. HPV DNA was detected and genotyped using polymerase chain reaction and enzyme immunoassay with GP5+/bio 6+ consensus specific primers and digoxigenin-labeled specific oligoprobes, respectively. The human ${\beta}$-globin gene was used as an internal control. Overall results represented that HPV frequency was 16/34 (47.1%) and 8/20 (40.0%) samples of vulva with cancer and abnormal cytology lesions, respectively, while, 3/5 (60%) and 16/33 (51.61%) samples of Vaginal cancer and abnormal cytology lesions, respectively, were HPV DNA positive. Single HPV type and multiple HPV type infection could be observed in both type of cancers and abnormal lesion samples in the different histological categorizes. HPV16 was the most frequent type in all cancers and abnormal cytology lesions, whereas HPV 18 was less frequent and could be detected as co-infection with other high risk HPV types. In addition, low risk types such as HPV 6, 11 and 70 could be detected in Vulva cancer and abnormal cytology lesion samples, whereas, all Vaginal cancer samples exhibited only high risk HPV types; HPV 16 and 31. In conclusion, from our results in this study we suggest that women with persistent high risk HPV type infection are at risk of developing vulva and Vaginal cancers and HPV 16 was observed at the highest frequent both of these, similar to the cervical cancer cases. Although the number of samples in this study was limited and might not represent the overall incidence and prevalence in Thai women, but the baseline data are of interest and suggest further study for primary cancer screening and/or developing the efficiency of prophylactic HPV vaccines in Thailand.

• 전자공학회논문지SC
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• 제48권2호
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• pp.64-70
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• 2011

휴대용 인공 전자 후각 시스템 (E-nose)의 가스 측정 환경은 실험실 내의 정교하게 제어되는 환경과 달리 온도, 농도, 기체 시료의 유속 등의 외부 요인의 변동이 매우 심하다. 이런 환경에서도 사용 가능한 단순하고 강인하고 정확한 가스 패턴 인식 알고리듬의 개발은 마이크로 바이오 센서의 발달과 함께 확대되고 있는 휴대용 및 소형 측정 진단 시스템에 있어 매우 중요하다. 본 연구에서는 PDA 기반의 휴대용 전자 후각 시스템을 활용해 실제 변화하는 환경에서 다채널 마이크로 센서로부터 감지되는 가스 신호를 수집하고, 여기에 영상 정합 기법을 적용하여 알고리듬의 강인성과 향상된 정확도를 검증하는 것을 목표로 하였다. 제안된 방법을 6종류의 가스 시료에 대한 7채널 마이크로 센서의 휴대 환경 측정 데이터에 적용하고, 기존의 최대 민감도 특징 추출 기법과 비교한 결과, 외부 환경의 변동에 영향 받지 않는 안정된 인식 성능 뿐 아니라 기존의 방법으로 구별하기 어렵던 2 종의 유사한 가스 시료에 대해서도 정확한 구분이 가능함을 보였다. 제안된 방법은 다양한 환경 변화에 노출되는 유비쿼터스 센서 네트워크 (USN)의 데이터 처리에도 쉽게 응용될 수 있을 것이며, 응용 현장에서 높은 안정성과 정확성을 요구하는 휴대용 의료 진단, 환경 감지 기술의 실용화에 큰 도움을 줄 수 있을 것으로 기대한다.

• 생태와환경
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• 제53권3호
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• pp.221-228
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• 2020

멸종위기 양서류 금개구리의 생물학적 특성 및 증식을 위한 기초자료 확보를 위해 난 발생 및 올챙이 초기 먹이 종류에 따른 영향에 대한 연구를 수행하였다. 사육수조 내에서 자연산란한 금개구리의 수정란을 현미경 하에서 관찰하였다. 수정란은 교질층에 둘러싸인 구형으로, 동물극과 식물극이 명확하게 구분되었다. 수온 27.0±1.0℃에서 수정 후 31시간 뒤에 부화하였으며, 수정 후 175시간 뒤에 아가미 뚜껑을 완성하였다. 개구 이후 단계부터 35일간 먹이종류별 성장 및 생존율을 분석한 결과 식물성 사료와 테트라민 단독 공급군의 평균 증체율(각각 5843.9%, 5736.3%), 평균 일일성장률 (각각 11.67%, 11.62%)이 다른 공급군들 (증체율: 641.8~4625.2%, 일일성장률: 5.72~11.01%)에 비해 가장 높았고(P<0.05), 야채 공급군이 가장 낮았다(P<0.05). 평균 사료 효율은 식물성 사료공급군이 177.83%로 야채 공급군 10.29%에 비해 약 17배 높았다. 또한 식물성 사료와 테트라민 단독 공급군의 평균생존율은 각각 97.5%, 100%로 가장 높았으며, 야채 공급군은 32.5%로 가장 낮았다. 따라서 금개구리의 성공적인 증식을 위한 올챙이 먹이는 식물성 사료 또는 테트라민을 단일로 공급하는 것이 가장 효율적인 것으로 판단된다.