• 한국환경보건학회:학술대회논문집
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• 한국환경보건학회 2003년도 Challenges and Achievements in Environmental Health
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• pp.116-122
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• 2003

The physicochemical properties of fungal chitosan at 95$^{\circ}C$ and 40$^{\circ}C$ acid treatment was as follows respectively. The nitrogen content was 6.71%, 6.91%, the viscosity 2.23cps, 2.21cps, the acetylation 12.0%, 12.7% and the molecular weight 3.12${\times}$10$\^$5/ Dalton, 3.01${\times}$10$\^$5/ Dalton. The absorbency band of reference, FCs-40 and FCs-95 in I.R. spectra was almost in accord with one another. In solid state NMR spectra, methyl group(-CH$_3$) was observed lightly. That means which deacetylation was well occurred. Carbonyl group(C=O) was not observed. C$_1$ to C$\_$6/ in solid state NMR was well observed seperately enough.

• 한국자원식물학회지
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• 제24권3호
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• pp.280-285
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• 2011

In this study, we established a novel somatic embryogenesis and plant regeneration system through cell suspension culture of white dandelion (Taraxacum coreanum NAKAI.). Embryogenic calli could be initiated from leaf and root explants of sterile seedlings on solid Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) after 3-week cultures. To proliferate embryogenic calli rapidly, cell suspension culture was performed with transferred to liquid MS medium with various combinations of plant growth regulators (PGRs) including 2,4-D, ${\alpha}$-naphthalene acetic acid (NAA), indole-3-acetic acid (IAA), $N^6$-benzylamino purine (BAP), thidiazuron (TDZ), and kinetin. During suspension cultures, embryogenic calli not only greatly proliferated, but shoot organogenesis also simultaneously occurred from the surface of somatic embryos. Among them, TDZ at lower concentration, 0.1 mg/L produced the highest efficiency of somatic embryo formation and shoot organogenesis. Rooting of embryogenic calli with adventitious shoots was done on solid MS medium containing 0.1 mg/L NAA and 0.3% activated carbon. Nearly 80% of embryogenic calli with shoot organogenesis could be rooted normal. Well-rooted plantlets were transferred into pots under a greenhouse condition, and plants derived from this system appeared phenotypically normal.

• 한국태양에너지학회 논문집
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• 제36권1호
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• pp.19-26
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• 2016

Biomass has been used for energy sources from the prehistoric age. Biomass are converted into solid, liquid or gaseous fuels and are used for heating, electricity generation or for transportation recently. Solid biofuels such as bio-chips or bio-pellet are used for heating or electricity generation. Liquid biofuels such as biodiesel and bioethanol from sugars or lignocellulosics are well known renewable transportation fuels. biogas produced from organic waste are also used for heating, generation and vehicles. Biomass resources for the production of above mentioned biofuels are classified under following 4 categories, such as forest biomass, agricultural residue biomass, livestock manure and municipal organic wastes. The energy potential of those biomass resources existing in Korea are estimated. The energy potential for dry biomass (forest, agricultural, municipal waste) were estimated from their heating value contained, whereas energy potential of wet biomass (livestock manure, food waste, waste sludge) is calculated from the biological methane potential of them on annual basis. Biomass resources potential of those 4 categories in Korea are estimated to be as follows. Forest biomass 355.602 million TOE, agricultural biomass 4.019 million TOE, livestock manure biomass 1.455 million TOE, and municipal organic waste 1.074 million TOE are available for biofuels production annually.

• 식품보건융합연구
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• 제10권3호
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• pp.19-22
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• 2024

The field of synthetic biology has emerged in response to the ongoing progress in the life sciences. Advances have been made in medicine, farming, eating, making materials, and more. Synthetic biology is the exploration of using living organisms to create new organisms. By manipulating specific genes to express targeted proteins, proteins can be created that are both productive and cost-effective. Solid-phase extraction (SPE) and liquid-liquid extraction (LLE) are employed for protein separation during the production process involving microorganisms. This study centers on Scanning Probe Microscopy (SPM) to showcase its utility in the food industry and food management. SPE is predominantly utilized as a pretreatment method to eliminate impurities from samples. In comparison to LLE, this method presents benefits such as decreased time and labor requirements, streamlined solvent extraction, automation capabilities, and compatibility with various other analytical instruments. Anion exchange chromatography (AEC) utilizes a similar methodology. Pharmaceutical companies utilize these technologies to improve the purity of biopharmaceuticals, thereby guaranteeing their quality. Used in the food and beverage industry to test chemical properties of raw materials and finished products. This exemplifies the potential of these technologies to enhance industrial development and broaden the scope of applications in synthetic biology.

• 유기물자원화
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• 제30권2호
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• pp.29-42
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• 2022

정부는 최근 2050년까지 국가 온실가스 순배출량은 "0"으로 하는 2050 탄소중립 정책을 추진하고 있다. 이에, 축산업 부문 2050 탄소중립을 위한 핵심적인 수단으로 가축분뇨 에너지화에 대한 관심이 증가하고 있다. 가축분뇨 고체연료화 기술은 가축분뇨를 건조 또는 탄화시켜 고체연료로 제조하는 기술로서 가축분뇨 고체연료 제조시설 및 발전시설의 경제성 문제로 인하여 보급 확산이 미미한 상황이다. 본 연구에서는 가축분뇨 고체연료 제조 및 이용 촉진을 위하여 가축분뇨 고체연료의 잠재적인 수요처인 가축분뇨 고체연료 발전시설의 경제성을 평가하고자 하였다. 가축분뇨 고체연료 제조원가는 200톤/일 규모의 발효건조 과정에서 97.4 천원/톤으로 가장 낮은 제조원가를 보였다. 가축분뇨 고체연료 발전시설은 신재생에너지공급인증서(REC) 가중치 1.5에서 경제성을 나타냈다. 가축분뇨 고체연료화는 온실가스 저감, 수계 비점오염원 저감 등 다양한 환경적 편익을 지니고 있다. 따라서 가축분뇨 고체연료 생산시설을 확산 보급을 위해서는 다양한 환경 편익을 포함한 경제성 검토가 요구된다.

• 상하수도학회지
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• 제25권2호
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• pp.257-264
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• 2011

Recycling of industrial products as the stabilizers can be proper handling of industrial products and has positive side in terms of recycling of wastes. In this study, the final aims were to evaluate the usability as stabilizer of Bio-solids which was generated from contaminated soil with heavy metals after primary process and to compare the treatment efficiency with slag being currently applied in many existing sites. Soluble and exchangeable forms have closely related to pollution of groundwater and plant growth and they can be used to determine the effect of the stabilization efficiency. Slag and Bio-solids were tested to investigate the capacity of stabilizing arsenic. Slag treatment process 4 (PS-ball 5%) showed higher leachate concentration rather to 0.84% compared to treatment 1 (blank) based on an average of 0.63%. The other hand treatment 4 (Bio-solids 5%) showed the lowest soluble and exchangeable forms to 0.57% when Bio-solids was applied to stabilize arsenic. Thus, the leaching of arsenic will be more reduced if the Bio-solids are used as stabilizer in stead of slag which is being currently used in many fields.

• Journal of Microbiology and Biotechnology
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• 제29권10호
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• pp.1624-1628
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• 2019

Banana planting altered microbial communities and induced the enrichment of Fusarium oxysporum in rhizosphere compared with that of forest soil. Diseased plant rhizosphere soil (WR) harbored increased pathogen abundance and showed distinct microbial structures from healthy plant rhizosphere soil (HR). The enriched taxon of Bordetella and key taxon of Chaetomium together with some other taxa showed negative associations with pathogen in HR, indicating their importance in pathogen inhibition. Furthermore, a more stable microbiota was observed in HR than in WR. Taken together, the lower pathogen abundance, specific beneficial microbial taxa and stable microbiota contributed to disease suppression.

• Environmental Engineering Research
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• 제14권4호
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• pp.250-254
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• 2009

Contamination of microcystins, a family of heptapeptide hepatotoxins, in eutrophic water bodies is a worldwide problem. Due to their poisoning effects on animals and humans, there is a requirement to characterize and quantify all microcystins present in a sample. As microcystins are, for most part, intracellular toxins produced by some genera of cyanobacteria, lysing cyanobacterial cells to release all microcystins is considered an important step. To date, although many cell lysis methods have been used, little work has been conducted comparing the results of those different methods. In this study, various methods for cell lysis and toxin extraction from the cell lysates were investigated, including sonication, bead beating, freeze/thaw, lyophilization and lysing with TritonX-100 surfactant. It was found that lyophilization, followed by extraction with 75% methanol, was the most effective for extracting toxins from Microcystis aeruginosa cells. Another important step prior to the analysis is removing impurities and concentrating the target analyte. For these purposes, a C18 Sep-Pak solid phase extraction cartridge was used, with the percentage of the eluent methanol also evaluated. As a result, methanol percentages higher than 75% appeared to be the best eluting solvent in terms of microcystin-leucine-arginine (MC-LR) recovery efficiency for the further chromatographic and mass spectrometric analyses.

• 한국식품저장유통학회지
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• 제5권4호
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• pp.315-319
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• 1998

유통을 위한 포도 보존 중 품질에 미치는 영향을 구명하여 보존기간 연장에 효과적인 적정 포장재 제작에 대한 기초자료를 얻고자 포도 캠벨얼리를 공시하여 스치로폴 상자, 마닐라상자 등 6처리를 두어 시험한 결과, 중량감소는 유통일수가 경과함에 따라 많았으며, 처리 간에는 15일 보존시 스치로폴상자에 비해 스치로폴상자+(스치로폴접시 + BioPE 필름밀봉), 스티로폴상자 + (스치로폴접시 + 랩 밀봉)처리가 적었으며, 비정상과는 밀봉구에서 많았으나, 축과는 밀봉으로 인한 수분증발 억제로 인하여 오히려 감소하였다. 외관상 품질 및 식미는 보존기간이 경과할수록 저하되었으나, 5일 보존 시에는 바이오상자 처리 포도의 품질이 우수하였고, 경도는 스티로폴상자 + (스치로폴접시 + 바이오필름) 처리에서 감소 폭이 가장 적었으며, 성분변화는 보존기간 경과에 따른 일정한 경향이 없었으며, 밀봉처리에서 당도의 감소 폭이 컷다.

• 한국연소학회:학술대회논문집
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• 한국연소학회 2013년도 제46회 KOSCO SYMPOSIUM 초록집
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• pp.61-64
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• 2013

Biomass is well known for organic resources photosynthesized by carbon dioxide water in the air and thus it can be widely used in the form of energy and production for various kinds of materials. Through pyrolysis, biomass can be transformed into solid(biochar), liquid(bio-oil), and combustible gas on the different condition of temperature and heating rate. That's why biomass can be practically used to preprocess and produce a variety of elements. This work is to analyze the characteristics of slow pyrolysis of three different kinds of biomass extracted from Indonesia. They showed similar moisture content and combinations of combustible matters and had quite a large discrepancy in the ash among them like 2.1 & of Bagasse, 91% of PKS, and 20.9% of Paddy Straw, respectively. yield of biochar, solid form of the biomass, steadily decreased when the temperature went up and that of bio-oil the highest at the temperature of 500 degrees Celsius. At the same temperature range, PKS bio-oil showed 51.4 % of yield and Bagasse had 55.1% while it turned out that Paddy straw showed the lowest yield of 37.2%. The apparent density was also measured to figure out the density of each product from the pyrolysis experiments at the temperature of 500 degrees Celsius. The result was like these; the density of biochar was 0.17, the lowest, and that of Tree stem was 1.3 when mixed by an equal amount of biochar and bio-oil.