The Korean Journal of Food & Health Convergence (식품보건융합연구)

Korea Food & Health Convergence Association (한국식품보건융합학회)
권호 표지
DOI QR코드

DOI QR Code

The High-throughput Solid-Phase Extraction in the Field of Synthetic Biology: Applications for the Food Industry and Food Managements

  • Hyeri SEONG (Laboratory of Microbial Physiology and Biotechnology, Department of Food and Nutrition, College of Bio-Convergence, and Institute of Food and Nutrition Science, Eulji University) ;
  • Min-Kyu KWAK (Laboratory of Microbial Physiology and Biotechnology, Department of Food and Nutrition, College of Bio-Convergence, and Institute of Food and Nutrition Science, Eulji University)
  • Received : 2024.05.24
  • Accepted : 2024.06.05
  • Published : 2024.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

The field of synthetic biology has emerged in response to the ongoing progress in the life sciences. Advances have been made in medicine, farming, eating, making materials, and more. Synthetic biology is the exploration of using living organisms to create new organisms. By manipulating specific genes to express targeted proteins, proteins can be created that are both productive and cost-effective. Solid-phase extraction (SPE) and liquid-liquid extraction (LLE) are employed for protein separation during the production process involving microorganisms. This study centers on Scanning Probe Microscopy (SPM) to showcase its utility in the food industry and food management. SPE is predominantly utilized as a pretreatment method to eliminate impurities from samples. In comparison to LLE, this method presents benefits such as decreased time and labor requirements, streamlined solvent extraction, automation capabilities, and compatibility with various other analytical instruments. Anion exchange chromatography (AEC) utilizes a similar methodology. Pharmaceutical companies utilize these technologies to improve the purity of biopharmaceuticals, thereby guaranteeing their quality. Used in the food and beverage industry to test chemical properties of raw materials and finished products. This exemplifies the potential of these technologies to enhance industrial development and broaden the scope of applications in synthetic biology.

Keywords

Acknowledgement

This work was supported by the BK21 Plus project of the National Research Foundation of Korea (NRF) Grant funded by the Korea government. This research was also supported by the National Research Foundation of Korea (NRF) grant funded by the Ministry of Science and ICT of the Korea government (MSIT) (NRF-2021R1F1A1061581) and by a grant from Eulji University in 2023. The funders had no role in study design; in the collection, analysis, and interpretation of data; in the writing of the report; and in the decision to submit the article for publication.

References

  1. Badawy, M. E., El-Nouby, M. A., Kimani, P. K., Lim, L. W., & Rabea, E. I. (2022). A review of the modern principles and applications of solid-phase extraction techniques in chromatographic analysis. Analytical Sciences, 38(11), 1457-1487.
  2. Benner, S. A., & Sismour, A. M. (2005). Synthetic biology. Nature Reviews Genetics, 6(7), 533-543.
  3. Bian, K., Gerber, C., Heinrich, A. J., Muller, D. J., Scheuring, S., & Jiang, Y. (2021). Scanning probe microscopy. Nature Reviews Methods Primers, 1, 36.
  4. Bigard, A., Cardinael, P., & Agasse, V. (2023). Anion exchange chromatography coupled to electrospray-mass spectrometry: An efficient tool for food, environment, and biological analysis. Critical Reviews in Analytical Chemistry, 53(2), 1591-1603.
  5. Clarke, L., & Kitney, R. (2020). Developing synthetic biology for industrial biotechnology applications. Biochemical Society Transactions, 48(2), 113-122.
  6. Hamidi, S., Taghvimi, A., & Mazouchi, N. (2021). Micro solid phase extraction using novel adsorbents. Critical Reviews in Analytical Chemistry, 51(2), 103-114.
  7. Kwak, M.-K., Liu, R., & Kang, S.-O. (2018). Antimicrobial activity of cyclic dipeptides produced by Lactobacillus plantarum LBP-K10 against multidrug-resistant bacteria, pathogenic fungi, and influenza A virus. Food Control, 85, 223-234.
  8. Lee, K. H., & Kim, D. M. (2013). Applications of cell-free protein synthesis in synthetic biology: Interfacing bio-machinery with synthetic environments. Biotechnology Journal, 8(11), 1292-1300.
  9. Li, K. M., Rivory, L. P., & Clarke, S. J. (2006). Solid-phase extraction (SPE) techniques for sample preparation in clinical and pharmaceutical analysis: A brief overview. Current Pharmaceutical Analysis, 2(1), 95-102.
  10. Meng, F., & Ellis, T. (2020). The second decade of synthetic biology: 2010-2020. Nature Communications, 11, 5174.
  11. O tles, S., & Kartal, C. (2016). Solid-phase extraction (SPE): Principles and applications in food samples. Acta Scientiarum Polonorum Technologia Alimentaria, 15(1), 5-15.
  12. Plotka-Wasylka, J., Marc, M., Szczepanska, N., & Namiesnik, J. (2017). New polymeric materials for solid phase extraction. Critical Reviews in Analytical Chemistry, 47(4), 373-383.
  13. Ridgway, K., Lalljie, S. P., & Smith, R. M. (2007). Sample preparation techniques for the determination of trace residues and contaminants in foods. Journal of Chromatography A, 1153, 36-53.
  14. Silva, M. S., Tavares, A. P., de Faria, H. D., Sales, M. G. F., & Figueiredo, E. C. (2022). Molecularly imprinted solid phase extraction aiding the analysis of disease biomarkers. Critical Reviews in Analytical Chemistry, 52(8), 933-948.
  15. Thurman, E. M., & Mills, M. S. (1998). Solid-phase extraction: Principles and practice. Wiley.