• 환경생물
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• 제40권4호
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• pp.433-441
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• 2022

Bacterial phytopathogen Pectobacterium causes soft rot disease in several vegetable crops globally, resulting in heavy agricultural losses at both the pre and postharvest stages. The present work was carried out to screen Kimchi cabbage genetic resources conserved at the National Agrobiodiversity Center, Rural Development Administration, Korea, for resistance against the soft rot pathogen Pectobacterium carotovorum subsp. carotovorum KACC 21701 over a period of three years (from 2020 to 2022). Infection of the phytopathogen was carried out at four-leaf stage and for each accession, twenty-five plants per germplasm were infected with KACC 21701. Kimchi cabbage cultivars Wangmatbaechu, Seoulbaechu, and CR Kiyoshi were used as control. Seven-days post-infection, the Disease Index (DI) values were manually recorded from zero to four, zero matched perfectly heathy plants and four completely dead plants. The 682 accessions of Kimchi cabbage exhibited varying degrees of disease resistance to KACC 21701 and thirty accessions, exhibiting a DI≤2, were considered for replication studies. During the replication studies, four landrace germplasms (IT102883, IT120036, IT120044, and IT120048) and one cultivar(IT187919) were confirmed to be moderately susceptible to KACC 21701. Results of the preliminary screening as well as replication studies were documented for the all the 682 germplasms. Addition of such information to the passport data of stored germplasms might serve as potential bio-resource for future breeders and researchers to develop resistant varieties or study the mechanisms involved in resistance of plants to such phytopathogen.

• 한국해양생명과학회지
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• 제3권2호
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• pp.81-86
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• 2018

The purpose of this study is to determine the effects of vibration on primary (e.g. plasma cortisol), secondary (e.g. plasma glucose, aspartate aminotransferase (AST), alanine aminotransferase (ALT), Na⁺, K⁺ and Cl^- and tertiary (e.g. mortality) stress responses in cultured eel, Anguilla japonica. For this purpose, three groups (including one control group and two stress groups) were set up. The control group was made exposed to vibration corresponding to 48 decibel (dB, V) (produced using electric vibrators) for 15 minutes per hour every day, and the two stress groups was made exposed to vibration corresponding to 58 and 68 dB (V) (produced using the same electric vibrators), equally, for 15 minutes per hour every day. Blood was sampled at day 0 (before starting vibration stress tests, BS), and days 1, 3, 5, 7, 9 and 11 (after starting vibration stress tests). As a result, plasma cortisol showed trend to continuously rise by consecutive stress from 4.1±0.1 ng/ml in BS. In 48 dB group (control), cortisol showed the highest level with 7.6±0.9 ng/ml after 7 days (p<0.05), but at 9 and 11 days was not significantly compared with BS level. In 58 dB group, the cortisol showed the highest level with 43.1±4.8 ng/ml after 1st day. Cortisol of 68 dB group increased significantly during the experimental period (14.4±2.3~32.0±5.7 ng/ml) (p<0.05). In 58, and 68 dB groups during the experimental period differed significantly compared to 48 group (p<0.05). Glucose in 48 dB were increased from 42.0 ±5.7 (BS) to 52.5±2.1 (1 day), the level was not significantly from 1 to 11 days. Glucose in 58 and 68 dB groups was increased significantly than BS during experimental period (p<0.05). K⁺ in 68 dB increased significantly (p<0.05) from 2.3±0.2 mE/ql (BS) to 3.3±0.5 mE/ql at 5 days. In 48 and 58 dB groups during the experimental period differed significantly (p<0.05). Na⁺ and Cl^- levels were not differed significantly during the experimental period. AST and ALT in 58 and 68 dB groups showed trend to continuously rise by consecutive stress. At 7 and 9 days in AST, between 48, 58 and 68 dB groups differed significantly (p<0.05). In 48, 58 and 68 dB groups at 1 day, blood hematocrit increased significantly higher than BS. The 11 days after vibration stress, the mortality in 48, 58 and 68 dB groups was 1.1, 5.1 and 5.8%, respectively. The present results have shown that A. japonica exhibited ''typical'' physiological responses when exposed to chronic vibration stress. These data suggested that chronic vibration stress caused substantial stress in the fish; especially the persisting elevated plasma AST and ALT levels observed would be expected to adverse effect. In conclusion, chronic vibration stress could greatly affects the hematological characteristics in A. japonica.

• 대한토목학회논문집
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• 제30권1B호
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• pp.101-105
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• 2010

해바라기(Sunking4505)와 유채(선망)는 고농도 올레인산을 함유한 유지종으로 바이오디젤 생산 원료로 사용되며, 식물정화공법(phytoremediation)에 사용되는 대표적 식물종이다. 본 연구는 이 식물종을 이용하여 카드뮴이 발아율 및 생육량에 미치는 영향을 평가하였다. 대상 식물종의 씨앗은 카드뮴용액(0, 1.5, 4, 12, 30, 60, 100, 150, 300, 500 mg/L)에서 7일간 배양 후 발아율, 유근과 지상부, 유묘 길이, 건조중량을 측정하였으며, 이를 이용하여 $IC_{50}$, 식물활성도, 내성지수를 구하였다. 또, 각 데이터는 분산분석(ANOVA)를 사용하여 유의성을 검증하였다. 건조중량을 제외한 발아율과 생육량은 카드뮴 농도가 증가함에 따라 감소하였다. 해바라기와 유채의 $IC_{50}$은 112, 10 mg-Cd/L이고, 해바라기씨는 500 mg-Cd/L에서 1개만 발아하였고, 유채씨는 150 mg-Cd/L 이상의 농도에서는 발아하지 못하였다. 대상 식물의 뿌리는 지상부보다 카드뮴에 대해 높은 민감도를 보였다. 해바라기의 발아율과 생육량, 식물활성도는 유채보다 높은 값을 가졌고, 내성지표는 상대적으로 저농도에서 유채보다 낮은 값을 가졌으나, 높은 농도에서는 유채가 발아하지 못해 높은 값을 보였다.

• 한국자연치유학회지
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• 제7권2호
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• pp.51-62
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• 2018

목적: 제주도 교래자연휴양림을 탐방한 대상자들 중 49명은 삼림욕을 한 후에 스트레스와 누적스트레스의 변화를 측정하였고, 또한 대상자들 중 85명은 설문을 통해서 직무스트레스, 사회 심리적 스트레스 및 상태불안감의 해소 등에 대한 설문을 하여 스트레스 수준의 변화를 조사하는 것이 목적이었다. 방법: 유비맥파기를 이용하여 스트레스의 수준을 측정하였고, 설문을 실시하여 데이터를 도출하였다. 결과: 삼림욕 후 스트레스 수준의 변화에서는 대조군은 사후에 스트레스가 증가하여 유의성이 없었고, 실험군은 사전보다 사후에 감소하여 유의성이 있었다(p<.043). 또한 분산분석에서는 그룹간에는 유의성이 있었고(p<.049), 그룹과 사전사후의 평균치에서도 유의성이 있었다( p<.048). 누적 스트레스의 변화에서는 실험군은 사전보다 사후에 감소하여 유의성이 있었다(p<.02). 또한 분산분석에서는 그룹간에서는 유의한 감소차이가 있었고(p<.047), 그룹과 사전사후 간에서도 유의한 차이가 있었다(p<.002). 전체적으로 직무스트레스의 설문의 경우에 사전보다 사후에는 감소하여 유의성이 있었다(p<.001).물리환경 및 직무요구도는 유의성이 없었다. 직무자율성, 관계갈등, 조직체계 불공정성, 보상부적절 및 직장문화의 경우에도 스트레스가 감소하여 유의성이 있었다(p<.001). 사회 심리적 스트레스 및 상태특성 불안감은 사전보다 사후에 감소하여 유의성이 있었다(p< .001). 결론: 교래자연휴양림에서 삼림욕을 한 후에는 스트레스와 불안감도 모두 감소하여 유의성이 있게 나타나서 스트레스 해소를 위한 자연치유 장소로서 크게 도움이 되는 기초정보를 제공하고 있다.

• Journal of Veterinary Science
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• 제24권6호
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• pp.83.1-83.12
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• 2023

Background: Ellipticine (Ellip.) was recently reported to have beneficial effects on the differentiation of adipose-derived stem cells into mature chondrocyte-like cells. On the other hand, no practical results have been derived from the transplantation of bone marrow stem cells (BMSCs) in a rabbit osteoarthritis (OA) model. Objectives: This study examined whether autologous BMSCs incubated with ellipticine (Ellip.+BMSCs) could regenerate articular cartilage in rabbit OA, a model similar to degenerative arthritis in human beings. Methods: A portion of rabbit articular cartilage was surgically removed, and Ellip.+BMSCs were transplanted into the lesion area. After two and four weeks of treatment, the serum levels of proinflammatory cytokines, i.e., tumor necrosis factor α (TNF-α) and prostaglandin E2 (PGE2), were analyzed, while macroscopic and micro-computed tomography (CT) evaluations were conducted to determine the intensity of cartilage degeneration. Furthermore, immuno-blotting was performed to evaluate the mitogen-activated protein kinases, PI3K/Akt, and nuclear factor-κB (NF-κB) signaling in rabbit OA models. Histological staining was used to confirm the change in the pattern of collagen and proteoglycan in the articular cartilage matrix. Results: The transplantation of Ellip.+BMSCs elicited a chondroprotective effect by reducing the inflammatory factors (TNF-α, PGE2) in a time-dependent manner. Macroscopic observations, micro-CT, and histological staining revealed articular cartilage regeneration with the downregulation of matrix-metallo proteinases (MMPs), preventing articular cartilage degradation. Furthermore, histological observations confirmed a significant boost in the production of chondrocytes, collagen, and proteoglycan compared to the control group. Western blotting data revealed the downregulation of the p38, PI3K-Akt, and NF-κB inflammatory pathways to attenuate inflammation. Conclusions: The transplantation of Ellip.+BMSCs normalized the OA condition by boosting the recovery of degenerated articular cartilage and inhibiting the catabolic signaling pathway.

• 한국응용과학기술학회지
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• 제39권6호
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• pp.906-915
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• 2022

본 연구의 목적은 12주간 수중걷기운동이 비만 노인 여성의 신체조성, hs-CRP, HOMA-IR 및 혈압에 미치는 영향을 알아보기 위하여 실시하였다. 체지방률 30% 이상인 비만 노인 여성 중 운동군(10명), 대조군(10명)으로 총 20명으로 실시하였으며, 모든 검사항목은 동일한 방법과 조건으로 신체조성, hs-CRP, HOMA-IR 및 혈압을 사전, 사후 총 2회 측정하였다. 수중걷기운동은 12주간, 주 3회, 1회 50분, RPE 11-14 강도로 실시하였다. 측정변인에 대해 평균값과 표준편차(M±SD)를 산출한 후 사전 운동군과 대조군의 신체적 특성과 각 측정변인에 대한 동질성 검정을 위하여 독립 t-test를, 집단 내의 사전·사후 평균치 변화에 대한 차이 검증은 종속 t-test를, 집단 간 차이에 대한 주효과 검정 및 집단 간 시기 간 상호작용 효과는 반복측정 분산분석을 이용하였다. 본 연구결과 운동군에서 신체조성, 체지방률(p<.05), 인슐린(p<.01), HOMA-IR(p<.01) 및 수축기 혈압(p<.05)이 유의하게 감소하였다. 따라서 수중걷기운동이 비만 노인 여성의 비만 및 심혈관질환의 개선에 도움을 줄 수 있을 것으로 사료된다.

• 한국동물위생학회지
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• 제46권4호
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• pp.269-281
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• 2023

In this study, a new triplex real-time quantitative reverse transcription polymerase chain reaction (tqRT-PCR) assay was developed for the rapid and differential detection of three feline viral pathogens including feline calicivirus (FCV), feline herpesvirus 1 (FHV-1), and influenza A virus (IAV) in a single reaction. The assay specifically amplified three targeted viral genes with a detection limit of below 10 copies/reaction. The assay showed high repeatability and reproducibility, with intra- and inter-assay coefficients of variation of less than 1%. Based on the diagnostic results of the assay using 120 clinical samples obtained from cats with feline respiratory disease complex (FRDC)-suspected signs, the prevalence of FCV, FHV-1, or IAV was 43.3%, 22.5%, or 0%, respectively, indicating that the diagnostic sensitivity was comparable or superior to those of previously reported monoplex qRT-PCR/qPCR assays. The dual infection rate for FCV and FHV-1 was 8.3%. These results indicate that FCV and FHV-1 are widespread and that co-infection with FCV and FHV-1 frequently occur in the Korean cat population. The developed tqRT-PCR assay will serve as a promising tool for etiological and epidemiological studies of these three bacterial pathogens, and the prevalence data for three feline viruses obtained in this study will contribute to expanding knowledge about the epidemiology of FRDC in the current Korean cat population.

• IMMUNE NETWORK
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• 제22권2호
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• pp.17.1-17.14
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• 2022

We aimed to investigate associations of dietary diversity (DD) with gut microbial diversity and the development of hen's egg allergy (HEA) in infants. We enrolled 68 infants in a high-risk group and 32 infants in a control group based on a family history of allergic diseases. All infants were followed from birth until 12 months of age. We collected infant feeding data, and DD was defined using 3 measures: the World Health Organization definition of minimum DD, food group diversity, and food allergen diversity. Gut microbiome profiles and expression of cytokines were evaluated by bacterial 16S rRNA sequencing and real-time reverse transcriptase-polymerase chain reaction. High DD scores at 3 and 4 months were associated with a lower risk of developing HEA in the high-risk group, but not in the control group. In the high-risk group, high DD scores at 3, 4, and 5 months of age were associated with an increase in Chao1 index at 6 months. We found that the gene expression of IL-4, IL-5, IL-6, and IL-8 were higher among infants who had lower DD scores compared to those who had higher DD scores in high-risk infants. Additionally, high-risk infants with a higher FAD score at 5 months of age showed a reduced gene expression of IL-13. Increasing DD within 6 months of life may increase gut microbial diversity, and thus reduce the development of HEA in infants with a family history of allergic diseases.

• 농업과학연구
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• 제50권3호
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• pp.383-394
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• 2023

In Korea, the U.S. Tier-4 Final emission standards have been applied to agricultural machinery since 2015. This study was conducted to analyze the emission characteristics of agricultural tractors during plow tillage operations using PEMS (portable emissions measurement systems). The tractor working speed was set as M2 (5.95 km/h) and M3 (7.60 km/h), which was the most used gear stage during plow tillage operation. An engine idling test was conducted before the plow tillage operation was conducted because the level of emissions differed depending on the temperature of the engine (cold and hot states). The estimated level of emissions for the regular area (660 m²), which was the typical area of cultivation, was based on an implement width of 2.15 m and distance from the work area of 2.2 m. As a result, average emission of CO (carbon monoxide), THC (total hydrocarbons), NOx (nitric oxides), and PM (particulate matter) were approximately 6.17×10-2, 3.36×10^-4, 2.01×10^-4, and 6.85×10^-6 g/s, respectively. Based on the regular area, the total emission of CO, THC, NOx, and PM was 2.62, 3.76×10^-2, 1.63, and 2.59×10^-4 g, respectively. The results of total emission during plow tillage were compared to Tier 4 emission regulation limits. Tier 4 emission regulation limits means maximum value of the emission per consumption power (g/kWh), calculated as ratio of the emission and consumption power. Therefore, the total emission was converted to the emission per power using the rated power of the tractor. The emission per power was found to be satisfied below Tier 4 emission regulation limits for each emission gas. It is necessary to measure data by applying various test modes in the future and utilize them to calculate emission because the emission depends on various variables such as measurement environment and test mode.

• 유기물자원화
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• 제18권2호
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• pp.55-61
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• 2010

본 연구에서는 음식물 쓰레기, 종이류 및 나무류 쓰레기, 음식물 폐수, 도축폐기물이 포함된 폐기물 발생량 분포지도를 국가 및 지역단위로 작성하였다. 환경부의 음식물류 폐기물, 종이류, 나무류, 음식물 폐수발생량, 도축폐기물 폐수발생량 등 폐기물통계자료를 수집하여 데이터베이스를 구축하고 GIS를 이용하여 발생원별 분포도를 작성하였다. 음식물류 폐기물 매립, 소각, 재활용 등의 자료를 이용하여 시군단위 음식물 폐기물 매립 및 재활용 분포도를 작성하였고, 나무류 및 종이류도 시군단위로 소각 및 재활용 분포도를 작성하였다. 음식물류 폐기물의 경우 시설지역 자료를 이용하여 공공시설과 민간시설로 나누어 폐수발생량 분포도를 작성하였다. 또한 도축 폐기물 폐수 발생량 분포도를 광역 시도단위로 작성하였는데 도축 돼지의 폐수 발생량은 경기도지역에서 특히 높게 나타났다. 이들 결과를 통해 폐기물 발생원별 분포도는 정책입안자, 농민, 일반인에게 폐기물자원에 대한 정보를 가시적으로 전달 할 수 있고, 친환경농업정책 추진과 바이오에너지 이용에 정책 자료로 활용할 수 있을 것으로 기대된다.