• 제목/요약/키워드: Bio Sample

검색결과 397건 처리시간 0.026초

자이모그라피 기술의 문제점과 해결 (Problems and Solutions of Zymography Techniques)

  • 강대욱;최낙식
    • 생명과학회지
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    • 제29권12호
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    • pp.1408-1414
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    • 2019
  • 효소는 세제, 식품, 사료, 의약품 및 의료용 분야 등 산업 전반적인 응용 분야에서 널리 사용되고 있으며, 산업 제품 및 공정에서 주요 요인이다. 효소를 선별, 확인, 및 특성 분석을 위해 zymography 기술이 일상적으로 사용됩니다. Zymography 기술은 SDS-전기영동을 통해 단백질을 분리한 후 포함된 기질을 겔 상에서 분해하는 기능성 효소를 검출하는 데 널리 사용되는 단순하고 민감하며 정량화가 가능한 기술이다. 이 방법은 비 환원 조건하에서 SDS-전기영동 겔에서 전기영동에 의한 단백질의 분리와 겔 상에서 효소 활성을 검출하는 다목적 2 단계의 기술이다. 이는 SDS-전기영동 겔에 기질을 중합시키고 전기영동 분리 후 효소 반응 완충용액에서 복원된 가수분해 효소에 의해 분해되는 것을 기반으로 하는 기술이다. 미생물 배양액, 식물 추출물, 혈액, 조직 배양액, 식품 속 효소 및 메타 프로테옴을 포함한 어떤 종류의 생물학적 시료들을 zymography에 적용하고 분석이 가능하다. Zymography의 장점은 전처리 없이 혼합된 시료를 적용하여 SDS-전기영동 겔 상에서 활성을 지닌 단백질을 직접 육안으로 검출이 가능할 뿐만 아니라 나노그람(nanogram) 수준에서 활성을 확인이 가능하다. 그래서 이 zymography 기술은 다양한 분야에 응용이 가능하다. 하지만, 이러한 장점이 오히려 단점으로 작용하여 실험적 오류를 범할 수 있는 경우가 많다. 본 총설에서 zymography 기술의 장점, 단점, 및 문제점 해결에 관해서 서술하였다.

Analysis of Broken Rice Separation Efficiency of a Laboratory Indented Cylinder Separator

  • Kim, Myoung Ho;Park, Seung Je
    • Journal of Biosystems Engineering
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    • 제38권2호
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    • pp.95-102
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    • 2013
  • Purpose: Using a laboratory indented cylinder separator, broken rice separation experiments were conducted and the characteristics of the separation process were studied to provide information for developing a prototype indented cylinder broken rice separator. Methods: Rice (Ilmi variety) milled in a local RPC was used for the experiment. Rice kernels were classified into four groups according to their length l; whole kernels (I > 3.75 mm), semi-whole kernels (2.5 < I < 3.75 mm), broken kernels (1.75 < I < 2.5 mm), and foreign matters (I < 1.75 mm). A laboratory grain cleaner, Labofix '90 (Schmidt AG, Germany) was used for the experiments. Experiments were designed as a $4{\times}4$ factorial arrangement in randomized blocks with three replications. Cylinder rotational speeds (17, 34, 51, 68 rpm) and trough angles (15, 37.5, 60, $82.5^{\circ}$) were the two factors and feed rates (25, 50 kg/h), indent shapes (Us, $S_1$ type), and indent sizes (2.5, 3.75 mm) were treated as the blocks. Two 125 g samples and one 125 g sample were taken at the cylinder outlet and from the trough, respectively. The whole, semi-whole, and broken kernel weight ratio of the samples and feed was determined by a rice sizing device. From these weight ratios, purities, degrees of extraction and coefficient of separation efficiency were calculated. Results: Trough angle, cylinder speed, and their interaction on the coefficient of separation efficiency were statistically significant. Cylinder speed of 17, 34, and 51 rpm made the most effective separation when the trough angle was $15^{\circ}$ or $37.5^{\circ}$, $60^{\circ}$, and $82.5^{\circ}$, respectively. Maximum values of coefficient of separation efficiency were in the range of 60 to 70% except when the indent size was 2.5 mm and were recorded for the combinations of low cylinder speed (17 rpm) with medium trough angle ($37.5^{\circ}$ or $60^{\circ}$). Indent shape did not appear to make any noticeable difference in separation efficiency. Conclusions: Due to the interaction effect, the trough angle needs to be increased appropriately when an increase in cylinder speed is made if a rapid drop of effectiveness of separation should be avoided. In commercial applications, $S_1$ type indents are preferred because of their better manufacturability and easier maintenance. For successful separation of broken kernels, the indent size should be set slightly bigger than the actual sizes of broken kernels: an indent size of 3.0 mm for separating broken kernels shorter than 2.5 mm.

동절기 대향류형 환기장치의 온실 내 습도 조절 효과 분석 (Analysis on the Effect of Greenhouse Humidity Control by Counter-flow Ventilator in Winter)

  • 이태석;강금춘;장재경;백이;임류갑
    • 생물환경조절학회지
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    • 제29권3호
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    • pp.259-264
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    • 2020
  • 본 연구에서는 겨울철 보온으로 인해 야간 및 새벽에 상대습도가 높아지는 온실에 대향류형 환기장치를 설치하고 그 효과를 분석하였다. 대향류형 환기장치는 크기 0.96×0.65×0.82(W×D×H, m)의 케이스에 크기 0.54×0.40×0.75(W×D×H, m)의 PE소재의 열교환 소자와 급기와 배기를 위한 송풍팬(풍량 6,800㎥/h, 소비전력1.7kW) 2대가 내부에 있다. 환기장치는 총 2대를 이용하였으며 토마토의 적정 생육 환경을 고려하여 설정 습도를 80%하고 18시부터 익일 8시까지 온실 내 온도 및 습도를 측정하고 분석하였다. 관행 온실에서 야간 평균 온도 및 습도는 14.9℃, 82.8%, 시험구 온실에서 야간 평균 온습도는 15.1℃, 79.9%로 측정되어 온도는 0.1℃, 습도는 약 3% 차이가 났다. 온실 평균 온도 및 습도를 월별로 비교하고 독립표본 t검정을 통해 분석해 본 결과, 유의수준 1%에서 각 월의 온도는 차이가 없는 것으로 나타났으며 습도는 차이가 있다고 판단된다. 따라서 대향류형 환기장치의 사용이 온실의 습도를 관리하고 작물 생육에 적합환 환경을 조성하여 작물 생산성 향상에 도움을 줄 수 있다고 판단된다. 이 외에도 환기장치 사용에 따른 난방비 증가와 같은 손실적 요소와 이익적 요소를 복합적으로 고려한 추가적인 연구도 필요할 것으로 사료된다.

희석에 의한 우유 중 $Aflatoxin\;M_1$의 효소면역측정법 (An Enzyme-Linked Immunosorbent Assay for $Aflatoxin\;M_1$ in Cow's Milk without a Cleanup Procedure)

  • 손동화;임선희;이인원
    • 한국식품과학회지
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    • 제28권6호
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    • pp.1184-1187
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    • 1996
  • 우유 중에 존재할 수 있는 발암성 진균독소의 하나인 $aflatoxin{\;}M_1{\;}(AFM_1)$을 신속, 간편하게 분석할 수 있는 효소면역측정법(ELISA)을 개발하고자 하였다. 소혈청알부민에 공유결합한 $AFM_1\;(AFM_1-BSA)$을 토끼에 면역하여 항체를 생산하고 정제하였다. 이 항체의 유사독소와의 교차반응율은 29.9%이하의 비교적 낮은 교차율을 나타냈다. $AFM_1$의 검출을 위하여 확립한 직접경합ELISA (cdELISA)로 우유에 인위적으로 오염시킨 $AFM_1$을 정제과정없이 ELISA로 분석하는 경우, 우유를 PBS로 40%되게(2:3) 희석하였을 때 양호한 회수율을 보였다. 이 조건하에서 행한 ELISA의 $AFM_1$분석 회수율은 0.3-3.0 ng/ml의 오염농도 범위에서 농도별 회수율로 평균 113%, 그 분산은 8.2%였다. 본 연구에서 개발한 ELISA system은 우유 중의 0.5 ppb이상의 $AFM_1$을 정제과정없이 손쉽게 분석하는데 유용할 것으로 판단된다.

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Fabrication, Estimation and Trypsin Digestion Experiment of the Thermally Isolated Micro Teactor for Bio-chemical Reaction

  • Sim, Tae-Seok;Kim, Dae-Weon;Kim, Eun-Mi;Joo, Hwang-Soo;Lee, Kook-Nyung;Kim, Byung-Gee;Kim, Yong-Hyup;Kim, Yong-Kweon
    • JSTS:Journal of Semiconductor Technology and Science
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    • 제5권3호
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    • pp.149-158
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    • 2005
  • This paper describes design, fabrication, and application of the silicon based temperature controllable micro reactor. In order to achieve fast temperature variation and low energy consumption, reaction chamber of the micro reactor was thermally isolated by etching the highly conductive silicon around the reaction chamber. Compared with the model not having thermally isolated structure, the thermally isolated micro reactor showed enhanced thermal performances such as fast temperature variation and low energy consumption. The performance enhancements of the micro reactor due to etched holes were verified by thermal experiment and numerical analysis. Regarding to 42 percents reduction of the thermal mass achieved by the etched holes, approximately 4 times faster thermal variation and 5 times smaller energy consumption were acquired. The total size of the fabricated micro reactor was $37{\times}30{\times}1mm^{3}$. Microchannel and reaction chamber were formed on the silicon substrate. The openings of channel and chamber were covered by the glass substrate. The Pt electrodes for heater and sensor are fabricated on the backside of silicon substrate below the reaction chamber. The dimension of channel cross section was $200{\times}100{\mu}m^{2}$. The volume of reaction chamber was $4{\mu}l$. The temperature of the micro reactor was controlled and measured simultaneously with NI DAQ PCI-MIO-16E-l board and LabVIEW program. Finally, the fabricated micro reactor and the temperature control system were applied to the thermal denaturation and the trypsin digestion of protein. BSA(bovine serum albumin) was chosen for the test sample. It was successfully shown that BSA was successfully denatured at $75^{\circ}C$ for 1 min and digested by trypsin at $37^{\circ}C$ for 10 min.

가교된 히아루론산 막의 분해 특성 (Degradation Characteristics of Cross-linked Hyaluronic Acid Membrane)

  • 정성일;조구현
    • 멤브레인
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    • 제19권4호
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    • pp.310-316
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    • 2009
  • 조직 공학용 지지체로 사용할 목적으로 제조된 가교된 lactide/hyaluronic acid (LA/HA) 막의 분해 특성을 살펴보았다. 가교제 1,3-butadiene diepoxide (BD), 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)를 사용하여 얻어진 고분자 막을 $37^{\circ}C$로 조절된 항온조에서 증류수에 침전시켜 분해시켰다. 가교반응시 LA/HA 몰비가 작을수록, 가교제의 농도가 작을수록 생성된 고분자 막의 분해속도가 증가하였다. 분해될 때 막의 구조 변화를 살펴보기 위해 분해 전, 3일, 6일, 9일 후의 시료를 채취하여 핵자기 공명 분광법으로 분자 구조를 살펴보았다. EDC로 가교시킨 막의 경우 시간이 지남에 따라 HA-EDC 결합구조는 서서히 분해되는데 HA-LA 결합구조는 급격히 분해되어 6일 후에는 완전히 소멸되었다. BD로 가교시킨 막의 경우 가교된 결합 구조 모두 서서히 분해되었으며 3일, 6일이 지나면서 HA-BD 결합 구조는 원래의 89, 83%가 유지되었으나 HA-LA 결합 구조는 원래의 83, 65%로 유지되었다. 분해된 막을 전자 현미경으로 측정한 결과 분해 전후 표면에서 기공의 밀도는 크게 차이나지 않았으며, 표면과 측면의 구조도 크게 차이가 나지 않아 조직공학용 재료로써 사용할 때 아무런 문제가 없는 것으로 관찰되었다.

Lactobacillus casei로 발효한 톳 추출물의 항염증 활성 (Anti-inflammatory Activity of Hizikia fusiformis Extracts Fermented with Lactobacillus casei in LPS-stimulated RAW 264.7 Macrophages)

  • 문옥주;권명숙;배민주;안별님;;김미향;이상현;유기환;김육용;서영완;공창숙
    • KSBB Journal
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    • 제30권1호
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    • pp.38-43
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    • 2015
  • In this study, we investigated the anti-inflammatory effects of fermented Hizikia fusiformis extracts in lipopolysaccharide (LPS)-stimulated RAW 264.7 mouse macrophages. The fermentation was performed using Lactobacillus casei in mixture of carbon source at $30^{\circ}C$ for 30 days. The sample groups were prepared with/without L. casei group in order to demonstrate the anti-inflammatory activity of fermented H. fusiformis in regard to lactic acid bacteria. As a result, we confirmed the inhibitory effect of H. fusiformis extracts on LPS-stimulated NO production and expression of $TNF{\alpha}$, while it had no regulatory effect on the expression of iNOS, COX-2, $IL-1{\beta}$ and IL-6 as important inflammatory factors. However, L. casei fermented group significantly suppressed the expression of the above factors. In particular, the difference between the two groups in the matter of mRNA expression of iNOS, which is directly associated with NO production, indicated that the fermentation with lactic acid bacteria effectively suppressed NO production by regulating iNOS expression. Also, effective suppression of pro-inflammatory cytokines showed that the fermentation using L. casei may provide an increment towards extraction of active ingredients that are effective anti-inflammatory agents.

멸치 가공선 자숙폐액 Pepsin 가수분해물의 Angiotensin 전환효소 저해작용 (Angiotensin Converting Enzyme Inhibitory Activity in Peptic Hydrolysates of Cooking Discards from Anchovy Factory Ship)

  • 지청일;이지혜;박덕천;구연숙;김인수;이태기;정규진;박영호;김선봉
    • 한국식품과학회지
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    • 제34권3호
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    • pp.529-532
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    • 2002
  • 현재 우리나라 남해안에서 많이 가공되고 있는 멸치 자건품은 통상 멸치 가공선에서 어획 즉시 자숙처리를 하여 제조되고 있는데, 이때 가공부산물로써 다량 배출되는 자숙액은 현재로서는 이용가치가 없을 뿐만 아니라 그대로 바다에 폐기되어 해양오염까지 유발시키고 있는 실정이다. 그리하여 이들 멸치 자숙 폐액을 대상으로 기능성 조미소재로서의 이용가치를 살펴보기 위해 소화효소인 pepsin에 의한 가수분해와 겔크로마토그라피를 이용하여 angiotensin 전환효소(ACE)저해 peptide를 검색하고 그 효과를 살펴보았다. 먼저, 멸치 자숙 폐액의 단백질 농도에 따른 ACE 저해작용을 살펴 본 결과 시료 용액 $100\;{\mu}L$당 1 mg으로 하였을 때 51.3%의 저해능을 나타내었다. 한편, pepsin을 이용하여 가수분해할 경우, ACE 저해효과는 4시간째에 65.8%로써 가장 높게 나타내었다. 이 가수분해물을 Bio-gel P-2 column chromatography에 의하여 분리한 결과, $({P-1}{\sim}{P-5})$의 5개의 획분을 얻었고 ACE 저해효과가 가장 우수한 획분은 P-2($IC_{50}$=0.319 mg protein/mL)로 나타났으며 이 획분의 아미노산 조정을 살펴본 결과 aspartic acid, glutamic acid 및 glycine의 함량이 높았다.

브로콜리 새싹 용매 추출물의 항산화 및 면역조절 활성 (Anti-oxidative and Immunomodulating Activities of Solvent Extracts from Broccoli (Brassica oleracea) Sprouts)

  • 고종호;김훈;황종현;유광원
    • 한국식품영양학회지
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    • 제32권1호
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    • pp.1-10
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    • 2019
  • In order to examine the functionality of broccoli sprout (Brassica oleracea, BS), solvent extracts were prepared and their anti-oxidative and immunomodulating activities were compared with those of broccoli (B). EtOH extracts (E) were potently higher than hot-water extracts (HW) in the antioxidant contents and radical scavenging activity. In particular, the total polyphenolic contents in addition to ABTS and DPPH radical scavenging activity were significantly higher in EtOH extract of broccoli sprout (BS-E; 9.15 mg GAE/g, 4.52 mg AEAC/g, and 1.14 mg AEAC/g) compared with that of broccoli (B-E; 7.83 mg GAE/g, 3.63 mg AEAC/g, and 0.97 mg/AEAC/g). Whereas, total flavonoid content was significantly higher in B-E (1.60 mg QE/g) than BS-E (1.43 mg QE/g). Anti-inflammatory activity was investigated using LPS-induced cell line model at a concentration of $10{\sim}100{\mu}g/mL$, in which all solvent extracts of both broccoli sprouts and broccoli were not toxic to RAW 264.7 cell lines. In anti-inflammatory activity of broccoli sprouts, EtOH extracts also showed significantly more potent activity than hot-water extracts in all sample concentrations tested. In addition, BS-E ($100{\mu}g/mL$) inhibited nitric oxide (NO) and IL-6 production to 60.9% and 68.9% compared with the LPS inflammation group (without extracts), whereas B-E inhibited 49.6% and 54.9%. On the other hand, in immunostimulating activity by splenocytes and macrophages, hot-water extract showed significantly higher activity than EtOH extract. Especially, BS-HW stimulated the splenocyte proliferation (1.2-fold against saline group) and IFN-${\gamma}$ production (264.39 pg/mL) at $100{\mu}g/mL$, and the production of IL-6 (1.33-fold), IL-12 (1.09-fold) and TNF-${\alpha}$ (1.49-fold) from macrophages was also significantly enhanced over broccoli. In conclusion, broccoli sprouts showed more potent anti-oxidative, anti-inflammatory and immunostimulating activity than broccoli, suggesting the possibility of using broccoli sprouts as functional food materials.

농촌 과소화 대응을 위한 마을 단위 농촌생활서비스 평가 모델의 개발 및 분석 (Development and analysis of assessment model of a village-level rural living services for response to rural depopulation)

  • 홍상원;배승종;김동현;김수진;김정태;장태일
    • 농촌계획
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    • 제27권1호
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    • pp.57-70
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    • 2021
  • The degree of benefits of living services related to the quality of life can solve the depopulation problem, and it is necessary to be able to quantitatively analyze problems related to the quality of life in rural areas in order to cope with the rural depopulation. The purpose of this study was to develop the assessment model of a village-level rural living service that reflects the regional characteristics of rural villages to evaluate the level of rural living services for response rural depopulation. Based on the review of previous related studies, the evaluation index was composed of seven sectors of education, health, welfare, culture, environment, safety, and convenience, and the assessment model of a rural living service was established. This model was evaluated through a sample survey of 90 villages in Nonsan-si, Seongju-gun, and Pyeongchang-gun. As a result of the rural life services evaluation by Si and Gun, Seongju-gun, which is affected by nearby large cities, has the largest variation by village level and is assessed at a lower level overall than other Si and Gun. As a result of the rural life services evaluation by 7 sectors, in the case of health and welfare, low scores were shown in the assessment model, but the level of residents' satisfaction was mid-level. In particular, in the case of Seongju-gun, there were significant differences in the assessment model and the survey results of the level of residents' satisfaction in the health and welfare sectors due to the influence of nearby large cities. As a result of analyzing the number of villages corresponding to the top 30% and the bottom 30% of the evaluation results for each sector, it was analyzed that the villages with the highest evaluation results in Pyeongchang-gun in both the assessment model and the level of residents' satisfaction. It implies that quantitative analysis of data based index and accessibility as well as level satisfaction of residents are necessary.