• Title/Summary/Keyword: Bio Sample

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Measurement of Blood Oxygen Saturation System and LavVIEW Program Using Broad-band Light Source (광대역 광원을 이용한 혈중 산소포화도의 측정 시스템 및 랩뷰 프로그램)

  • Cui, Jin Shi;Shin, Dong Ho;Song, Chul Gyu
    • Journal of Korea Multimedia Society
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    • v.18 no.2
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    • pp.128-137
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    • 2015
  • Blood oxygen saturation ($SpO_2$) is so important to be called bio-signal of the fifth. The measurement of blood oxygen saturation based on broad-ban light source has advantages of simple testing facility and easy understanding. This paper proposes a LabVIEW program which measures blood oxygen saturation based on broad-band light source. It combines LabVIEW and MATLAB, utilizing different light absorptions of oxyhemoglobin and deoxyhemoglobin in the visual wavelength range of 450nm-750nm to determine blood oxygen saturation. In order to improve accuracy through reducing the impact of hand shaking, the probe is fixed to the motor stage and then move a constant distance between the probe and the sample to be measured. Experimental results show that the proposed method noticeably increases the accuracy and saves time compared with the conventional methods.

The influence of periapical lesion on furcation involvement in mandibular molars (하악 대구치에서 치근단 병소가 치근 이개부 감염에 미치는 영향)

  • Jang, Ji-Hye;Seo, Sung-Chan;Lee, Eun-Suk;Kim, Hyung-Seop
    • Journal of Periodontal and Implant Science
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    • v.35 no.1
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    • pp.177-185
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    • 2005
  • The purpose of the study was to investigate the influence of an endodontic infection on presence of furcation involvement in periodontally-involved mandibular molars. All first and second mandibualr molars in 45 patients were selected if at least one was root-filled or had a possible periapical radiolucency. The sample consisted of patients from a referral population at a periodotnal clinic which represented an adult population with a mean age of 47.5 years (range 31 to 63) For mandibular molars with periapical destruction at both roots, frequency of horizontal furcation depth ${\geqq}$ 3 mm was significantly more compared to teeth without periapical destruction. Mean periodontal probing depth was significantly greater at mandibular molars with periapical destruction. It is suggested that a root canal infection in periodontitis-involved molars may potentiate periodontitis progression by spreading of endodontic pathgens through patent accessory canals and dentinal tubules. In conclusion, an endodontic infection in mandibular molars was found to be associated with additional attachment loss in the furcation area, and may thus be considered to be one of several risk factors influencing the prognosis of molars in periodontitis-prone patients.

Investigation of friction effects between needles patterned using laser and elastomer (레이저에 의해 패터닝 된 바늘과 탄성중합체와의 마찰 효과)

  • Kim, Jae-Gu;Ro, Seung-Kook;Park, Jong-Kweon;Cho, Sung-Hak;Whang, Kyung-Hyun
    • Laser Solutions
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    • v.15 no.3
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    • pp.1-6
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    • 2012
  • The friction force of patterned needle in elastomer have been investigated to verify the application for bio and plastic industry. The micro pattern on the needle surface were prepared by 266 nm, 20 ns laser and 800 nm, 220 fs laser, which were able to generate the different surface roughness. The friction force was measured by the load cell of 10 N capacity. As the results, the friction force of no patterned needle is almost constant during the needle penetrates the silicone rubber sample. However, the needle having asperities shows the variation of the friction force. The higher the surface roughness is, the smaller the friction force is until the surface roughness is very high. In our experiment conditions, the reduction of the friction force by 20 % compared to no pattern needle was achieved with straight and $50{\mu}m$ discrete line generated by 266 nm, 20 ns laser.

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Antioxidation, Physicochemical, and Sensory Characteristics of Sulgidduck Fortified with Water Extracts from Moringa oleifera Leaf (모링가 잎 열수 추출물을 첨가한 설기떡의 항산화, 이화학 및 관능 특성)

  • Choi, Eun-Ju;Kim, Eun-Kyung
    • Korean journal of food and cookery science
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    • v.31 no.3
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    • pp.335-343
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    • 2015
  • The aim of this investigation was to examine the antioxidation, physicochemical, and sensory activity of a Korean steamed-rice cake, Sulgidduk, fortified with water extracts from Moringa oleifera (M. oleifera) leaf. M. oleifera leaf extracts were added to rice powder at rations of 0.1%, 1% and 10%. To examine antioxidation properties, the scavenging activities of DPPH radicals, hydroxyl radicals, ABTS+ radicals, and ferric ion reducing antioxidant power were investigated. M. oleifera extracts significantly increased the antioxidation activities of Sulgidduk in a dose dependent manner (p<0.05). Physicochemical characteristics were measured by proximate composition, color, texture profile analysis, and sensory evaluations. As the concentration of M. oleifera leaf extracts increased, L-values and a-values significantly decreased while b-values increased. Texture profile analysis demonstrated that the control groups showed significantly higher values for hardness, cohesiveness, chewiness, and adhesiveness as compared with groups containing M. oleifera leaf extract (p<0.05). In the sensory evaluation, the sample containing 0.1% of M. oleifera leaf extract obtained the best results in overall preference. Taken together, these results suggest that M. oleifera leaf may have the potential to increase the consumer acceptability and the functionality of Sulgidduk.

Simple Identification of DNA Samples Using Multiplex PCR (다중 중합효소연쇄반응을 이용한 핵산시료의 동정방법)

  • Park, Hwa-Yong;Yu, Hyun-Joo
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.22 no.2
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    • pp.427-430
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    • 2008
  • Serious controls and cares using ID numbers and barcode needed throughly to have appropriate management in clinical tissues and nucleic acids inventories because these samples are the most essential and important materials in the experimental research laboratories. While almost all of the laboratories using and handling DNA samples as starting materials in their research, problems such as mixing up of two or more different samples together, contamination with other samples, and/or mistakes can occur, especially when it comes with large number of samples. These problems are rather frequent even though researchers pay more attentions to be far away from these obstacles. It has been such a long time since PCR became useful as an important and essential biological research tool among lots of bio-scientific research methods. In this research, we tried to set up a simple and cost-effective genotyping method using PCR and agarose gels, instead of expensive automated machines, for identification and discrimination among those DNA samples, as a kind of low level quality control and sample inventory management.

A Study on the Characteristics of Salad Dressings Containing Chicken Foot Gelatin (닭발 추출 젤라틴을 이용한 샐러드 드레싱의 품질 특성)

  • Shin, Mee-Hye;Kim, Jong-Goon;Kang, Kun-Og
    • Journal of the East Asian Society of Dietary Life
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    • v.18 no.1
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    • pp.58-63
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    • 2008
  • This study was conducted to present fundamental data on the physicochemical properties and sensory qualities of salad dressings made with chicken foot gelatin. Preliminary experiments were performed to confirm the gelatin powder concentrations in preparing gelatin solutions. A 2% gelatin solution, including 0.5% agar, was prepared for use in the experiments that followed. Sensory evaluations were conducted to compare the organoleptic acceptance of dressings manufactured with differing concentrations of the additive in seasoning soybean sauce, mayonnaise, and sesame powder. The viscosities of the dressings significantly increased with increasing gelatin powder concentration. A decrease in turbidity was observed in the mayonnaise and sesame dressings. The color difference values of all dressings indicated no changes. In sensory evaluations of dressings prepared with gelatin solutions at different concentrations of 2 g (1%), 4 g (2%), and 8 g (4%) of gelatin powder, the 4 g (2%) sample received the highest score for overall acceptance. From this study, which was conducted to find an efficient use for chicken house wast product, it is anticipated that chicken feet will be utilized as a new raw material for producing collagen and gelatin, protein sources widely increasing in use with in the food and bio-industries.

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Development of loop-mediated isothermal amplification method for the rapid and sensitive detection of bovine tuberculosis in Korea native cattle (한우 결핵의 신속 감별진단을 위한 등온증폭법 개발)

  • Hwang, Eun-Suk;Lee, Tae-Uk;Jung, Dae-Young;Cho, Ho-Seong
    • Korean Journal of Veterinary Service
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    • v.34 no.4
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    • pp.333-339
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    • 2011
  • Loop-mediated isothermal amplification (LAMP) was developed to detect Mycobacterium tuberculosis complex (MTC) and non-tuberculous mycobacterium (NTM) genomic DNA in blood samples of Korea native cattle. A set of four primers, two outer and two inner, were designed from M. bovis and M. avium genomic DNA targeting the IS6110 and 16S rRNA gene, respectively. Based on 85 Intradermal Tuberculin Test (ITT) positive blood sample and using conventional PCR and LAMP, the agreement quotient (kappa), which measures agreement beyond chance were 0.93 (conventional PCR) and 0.97 (LAMP), respectively. The detection limit of the LAMP method was $2.0{\times}10^2$ copy/ml M. bovis and M. avium cells, compared to $2.0{\times}10^3$ copy/ml M. bovis and M. avium cells for conventional PCR. These results suggest that the LAMP is a powerful tool for rapid, sensitive, and practical detection of MTC and NTM in blood samples of Korea native cattle.

Quality Characteristics of Cookies with Added Dried Laver (Porphyra tenera) Powder (김 분말을 첨가한 쿠키의 품질특성)

  • Lee, Jeong-Ae;Song, Jung-Sun;Yoon, Ji-Young
    • Culinary science and hospitality research
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    • v.23 no.7
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    • pp.88-96
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    • 2017
  • This study investigated the quality characteristics of cookies containing laver powder, which is well known for its various functions and biological activity. This study assessed the quality characteristics of cookies containing various concentrations (0, 2, 4, 6 and 8%) of laver powder(LP). To analyze quality characteristics, pH of dough, moisture content, color, hardness, and sensory properties were measured. Qualities, such as pH, brightness, and redness and yellowness and hardness decreased significantly (p<0.001). The total flavonoid and total polyphenol and ABTS radical scavenging activity were proportionally increased with increasing the level of laver powder added in cookies(p<0.001). The antioxidant activity measured by total flavonoid and total polyphenol activities was significantly higher than control and increased proportionally to the laver powder. The result of sensory test shows that sample group adding 2% of laver powder achieved the highest. Based on the above results, this study suggests that the addition of 2% laver powder may be the best substitution ratio for cookies of the improvement of sensory preference and antioxidant. This study exhibited both the functional and health based benefits of laver when it is added to cookies and confirmed the development feasibility of laver cookies considering consumer satisfaction.

Abalone Protein Hydrolysates: Preparation, Angiotensin I Converting Enzyme Inhibition and Cellular Antioxidant Activity

  • Park, Soo Yeon;Je, Jae-Young;Hwang, Joung-Youl;Ahn, Chang-Bum
    • Preventive Nutrition and Food Science
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    • v.20 no.3
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    • pp.176-182
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    • 2015
  • Abalone protein was hydrolyzed by enzymatic hydrolysis and the optimal enzyme/substrate (E/S) ratios were determined. Abalone protein hydrolysates (APH) produced by Protamex at E/S ratio of 1:100 showed angiotensin I converting enzyme inhibitory activity with $IC_{50}$ of 0.46 mg/mL, and APH obtained by Flavourzyme at E/S ratio of 1:100 possessed the oxygen radical absorbance capacity value of $457.6{\mu}M$ trolox equivalent/mg sample. Flavourzyme abalone protein hydrolysates (FAPH) also exhibited $H_2O_2$ scavenging activity with $IC_{50}$ of 0.48 mg/mL and $Fe^{2+}$+ chelating activity with $IC_{50}$ of 2.26 mg/mL as well as high reducing power. FAPH significantly (P<0.05) protected $H_2O_2$-induced hepatic cell damage in cultured hepatocytes, and the cell viability was restored to 90.27% in the presence of FAPH. FAPH exhibited 46.20% intracellular ROS scavenging activity and 57.89% lipid peroxidation inhibition activity in cultured hepatocytes. Overall, APH may be useful as an ingredient for functional foods.

On-line Monitoring and Control of Substrate Concentrations in Biological Processes by Flow Injection Analysis Systems

  • Rhee, Jong-Il;Adnan Ritzka;Thomas Scheper
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.9 no.3
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    • pp.156-165
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    • 2004
  • Concentrations of substrates, glucose, and ammionia in biological processes have been on-line monitored by using glucose-flow injection (FIA) and ammonia-FIA systems. Based on the on-line monitored data the concentrations of substrates have been controlled by an on-off controller, a PID controller, and a neural network (NN) based controller. A simulation program has been developed to test the control quality of each controller and to estimate the control parameters. The on-off controller often produced high oscillations at the set point due to its low robustness. The control quality of a PID controller could have been improved by a high analysis frequency and by a short residence time of sample in a FIA system. A NN-based controller with 3 layers has been developed, and a 3(input)-2(hidden)-1(output) network structure has been found to be optimal for the NN-based controller. The performance of the three controllers has been tested in a simulated process as well as in a cultivation process of Saccharomyces cerevisiae, and the performance has also been compared to simulation results. The NN-based controller with the 3-2-1 network structure was robust and stable against some disturbances, such as a sudden injection of distilled water into a biological process.