• Title/Summary/Keyword: Binding property

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Anonymity-Based Authenticated Key Agreement with Full Binding Property

  • Hwang, Jung Yeon;Eom, Sungwook;Chang, Ku-Young;Lee, Pil Joong;Nyang, DaeHun
    • Journal of Communications and Networks
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    • v.18 no.2
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    • pp.190-200
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    • 2016
  • In this paper, we consider some aspects of binding properties that bind an anonymous user with messages. According to whether all the messages or some part of the messages are bound with an anonymous user, the protocol is said to satisfy the full binding property or the partial binding property, respectively. We propose methods to combine binding properties and anonymity-based authenticated key agreement protocols. Our protocol with the full binding property guarantees that while no participant's identity is revealed, a participant completes a key agreement protocol confirming that all the received messages came from the other participant. Our main idea is to use an anonymous signature scheme with a signer-controlled yet partially enforced linkability. Our protocols can be modified to provide additional properties, such as revocable anonymity. We formally prove that the constructed protocols are secure.

Screening of Lactic Acid Bacteria with Potent Adhesive Property in Human Colon using Colonic Mucin-binding Assay (Colonic mucin-binding assay를 이용한 장내 우수 점착능 유산균주의 선별)

  • Kim, Seong-Yeong;Shin, Kwang-Soon;Lee, Ho
    • Korean Journal of Food Science and Technology
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    • v.36 no.6
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    • pp.959-967
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    • 2004
  • To screen probiotic lactic acid bacteria with potent adhesive property on human colonic mucosa, colonic mucin-binding assay was introduced. This colonic mucin-binding assay actually measures the binding activity of surface lectin-like protein (SLP) on colonic mucin, and the optimal conditions were examined. The optimal pH for colonic mucin coating on plate wells was 4.8, and ${\times}24,000$ diluted solution of commercially available horseradish peroxidase (HRP) conjugated streptoavidin yielded good results, for rapid screening, $5.0\;{\mu}g/mL$ of biotinylated SLP from lactic acid bacteria was optimal, and optimal scintillation time of 3,3',5,5'-tetramethyl benzidine (TMB) was 10 min. These conditions were useful for both rapid selection and quantitative analysis of lactic acid bacteria that have high adhesion property to human intestinal tract. Among 50 strains of lactic acid bacteria, including 32 type culture strains and 18 isolated strains from infant feces, Lactobacillus species FSB-1 isolated from kimchi showed the highest binding activity to colonic mucin. From taxonomical viewpoints based on morphological study, physico-biochemical study, partial 16S rDNA seguencing, and phylogenetic analysis, L. species FSB-1 was identified as Lactobacillus brevis.

Leaf-specific pathogenesis-related 10 homolog, PgPR-10.3, shows in silico binding affinity with several biologically important molecules

  • Han, Jin Haeng;Lee, Jin Hee;Lee, Ok Ran
    • Journal of Ginseng Research
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    • v.39 no.4
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    • pp.406-413
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    • 2015
  • Background: Pathogenesis-related 10 (PR-10) proteins are small, cytosolic proteins with a similar three-dimensional structure. Crystal structures for several PR-10 homologs have similar overall folding patterns, with an unusually large internal cavity that is a binding site for biologically important molecules. Although structural information on PR-10 proteins is substantial, understanding of their biological function remains limited. Here, we showed that one of the PgPR-10 homologs, PgPR-10.3, shares binding properties with flavonoids, kinetin, emodin, deoxycholic acid, and ginsenoside Re (1 of the steroid glycosides). Methods: Gene expression patterns of PgPR-10.3 were analyzed by quantitative real-time PCR. The three-dimensional structure of PgPR-10 proteins was visualized by homology modeling, and docking to retrieve biologically active molecules was performed using AutoDock4 program. Results: Transcript levels of PgPR-10.3 expressed in leaves, stems, and roots of 3-wk-old ginseng plantlets were on average 86-fold lower than those of PgPR-10.2. In mature 2-yr-old ginseng plants, the mRNA of PgPR-10.3 is restricted to leaves. Ginsenoside Re production is especially prominent in leaves of Panax ginseng Meyer, and the binding property of PgPR-10.3 with ginsenoside Re suggests that this protein has an important role in the control of secondary metabolism. Conclusion: Although ginseng PR-10.3 gene is expressed in all organs of 3-wk-old plantlets, its expression is restricted to leaves in mature 2-yr-old ginseng plants. The putative binding property of PgPR-10.3 with Re is intriguing. Further verification of binding affinity with other biologically important molecules in the large hydrophobic cavity of PgPR-10.3 may provide an insight into the biological features of PR-10 proteins.

The Hydrogen Binding Property Study by Density Functional Theory for Zr, V, Fe and Al (밀도 함수를 이용한 지르코니움, 바나듐, 철과 수소와의 반응성 연구)

  • Park, Taesung;Lee, Taeckhong
    • Transactions of the Korean hydrogen and new energy society
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    • v.25 no.6
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    • pp.602-608
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    • 2014
  • The sequence of bond overlap population of metal hydrogen binding is in Al-H > Fe-H > Zr-H > V-H. This results shows the binding energy of Al-H is the biggest in this metals (Al, Fe, Zr, and V) and hydrogen interaction. The Vanadium-hydrogen binding shows the weakest binding energy compared to other metals and it causes easy hydrogen desorption from the corresponding metals. The net charge of Al-H show the biggest value of 0.2248 and the severe localizations of electrons around aluminum and imply strongest covalent binding nature in these metals. This study is applicable to the purification of hydrogen in other bulk gas.

Temperature dependent hydrogen exchange study of DNA duplexes containing binding sites for Arabidopsis TCP transcription factors

  • Kim, Hee-Eun;Choi, Yong-Geun;Lee, Ae-Ree;Seo, Yeo-Jin;Kwon, Mun-Young;Lee, Joon-Hwa
    • Journal of the Korean Magnetic Resonance Society
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    • v.18 no.2
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    • pp.52-57
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    • 2014
  • The TCP domain is a DNA-binding domain present in plant transcription factors and plays important roles in various biological functions. The hydrogen exchange rate constants of the imino protons were determined for the three DNA duplexes containing the DNA-binding sites for the TCP11, TCP15, and TCP20 transcription factors using NMR spectroscopy. The M11 duplex displays unique hydrogen exchange property of the five base pairs in the first binding site (5'-GTGGG-3'). However, the M15 and M20 duplexes lead to clear changes in thermal stabilities of these five base pairs. The unique dynamic features of the five base pairs in the first binding site might play crucial roles in the sequence-specific DNA binding of the class I TCP transcription factors.

The Experimental Analysis of Integrated (Name/Property) Dynamic Binding Service Model for Wide-Area Objects Computing (광역 객체 컴퓨팅에서 통합(이름/속성) 기반의 동적 바인딩 서비스 모델의 실험분석)

  • Jeong, Chang-Won;Joo, Su-Chong
    • Journal of KIISE:Computer Systems and Theory
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    • v.33 no.10
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    • pp.746-758
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    • 2006
  • Many objects existing on wide area environments have the replication characteristics according to how to categorize using their own names or properties. From the clients' requests, the existing naming and trading services have not supported with the binding service for replicated solver object with the same service type. For this reason, we present an integrated model that can support the selection of replicated object and dynamic binding services on wide-area computing environments. This model suggests provides not only location management of replicated objects but also active binding service which enables to select a least-loaded object on the system to keep the balance of load between systems. In this purpose, constructing both the service plan and model for support solver object's binding with replication property on wide area computing environments has been researched. In this paper, we showed the test environment and analyzed the performance evaluation of client/server binding procedures via integrated binding service in federation model and verified our model under the condition to see whether load balance can be applied to our model. For the performance evaluation of suggested wide area integrated binding service federation model, evaluated the integrated binding service of each domain and analyzed the performance evaluation of process for non-replication object's under federation model environment. Also, we analyzed the performance evaluation of the federation model between domains for wide area environment. From the execution results, we showed the federation model provides lowers search-cost on the physical tree structure of network.

Studies on the Correlation between Coated Paper and Physical Properties of Latices (라덱스의 물성이 도공지 품질에 미치는 상관성에 대한 연구)

  • 박동국;조교동;고문찬;윤재한;이용규
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.34 no.1
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    • pp.22-29
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    • 2002
  • The coated paper was greatly affected by the basic physical properties of the binder as well as the amount of the coating formula. High glass transition temperature (Tg) of the styrene-butadiene (SB) latex, selected as the binder in our study, gave the high stiffness to the coated paper, but lowered the binding force and print gloss. The average particle size of the SB latex also greatly affected to the coated paper so that the smaller particle size improved the rheological property of the coating formula and increased the binding force and print gloss. Another property of the SBR latex, gel content, was important because when its value was small, the latex was easily deformed at the high temperature and increased air permeability to the coated paper. Therefore, the lower gel content consequently resulted in the higher blistering resistance, especially in the web paper. The larger portion of the SB latex in the coated formula improved the binding force and print gloss, but decreased the ink set-off and ink-trapping to the coated paper. The heavier coating improved optical properties such as opacity, paper gloss and paper smoothness, to the coated paper.

Production of the recombinant fibronectin-binding protein of Staphylococcus aureus (Staphylococcus aureus의 재조합 fibronectin-binding protein의 생산)

  • kim, Doo;Cheong, Cha-ryong;Park, Hee-myong;Han, Hong-ryul
    • Korean Journal of Veterinary Research
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    • v.37 no.4
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    • pp.875-882
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    • 1997
  • To produce the recombinant fibronectin-binding protein(FnBP) for development of subunit vaccine against Staphylococcus aureus. The fnbp gene was amplified from the chromosomal DNA of S aureus KNU 196 strain using the polymerase chain reaction, and cloned into pGEX-4T-2. Then, the recombinant FnBP fused with glutathione-S-transferase was produced in E coli, purified by affinity chromatography, and identified its antigenicity and immunogenicity by Western blot. The recombinant FnBP produced in this study is considered to have the same property of native FnBP purified from S aureus, and is expected to be useful as a candidate for S aureus subunit vaccine.

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