• 제목/요약/키워드: Binding property

검색결과 221건 처리시간 0.027초

Anonymity-Based Authenticated Key Agreement with Full Binding Property

  • Hwang, Jung Yeon;Eom, Sungwook;Chang, Ku-Young;Lee, Pil Joong;Nyang, DaeHun
    • Journal of Communications and Networks
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    • 제18권2호
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    • pp.190-200
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    • 2016
  • In this paper, we consider some aspects of binding properties that bind an anonymous user with messages. According to whether all the messages or some part of the messages are bound with an anonymous user, the protocol is said to satisfy the full binding property or the partial binding property, respectively. We propose methods to combine binding properties and anonymity-based authenticated key agreement protocols. Our protocol with the full binding property guarantees that while no participant's identity is revealed, a participant completes a key agreement protocol confirming that all the received messages came from the other participant. Our main idea is to use an anonymous signature scheme with a signer-controlled yet partially enforced linkability. Our protocols can be modified to provide additional properties, such as revocable anonymity. We formally prove that the constructed protocols are secure.

Colonic mucin-binding assay를 이용한 장내 우수 점착능 유산균주의 선별 (Screening of Lactic Acid Bacteria with Potent Adhesive Property in Human Colon using Colonic Mucin-binding Assay)

  • 김성영;신광순;이호
    • 한국식품과학회지
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    • 제36권6호
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    • pp.959-967
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    • 2004
  • 인간의 대장내 점막에 대하여 우수한 점착특성을 갖는 probiotic 유산균주를 선별할 목적으로, colonic mucin-binding assay를 고안하고 최적의 분석 조건을 검토한 결과, microtiter plate의 well에 대한 colonic mucin의 부착은 pH 4.8, biotinylated SLP의 농도는 $5.0\;{\mu}g/mL$, 시판 HRP-conjugated streptoavidin은 24,000배 희석용액, TMB의 발색시간은 10분의 조건에서 측정시 최적의 결과를 나타냈다. 동 조건에서 본 assay system을 이용할 경우, 장내 점착능 측정 및 우수 유산균주의 선별에 있어 신속하고 재현성 있는 결과를 제공할 수 있으며, 인간의 대장에 대한 유산균의 점착특성을 정량적으로 분석할 수 있음을 보여주었다. 공시균주 32종 및 유아 분변 유래의 분리균주 18종을 포함한 총 50종의 유산균주에 대하여, colonic mucin-binding assay를 이용하여 대장 mucin에 대한 결합능을 비교한 결과, L. species FSB-1이 가장 높은 결합능을 보여주었다. 따라서 L. species FSB-1을 대상으로 형태학적 특성, 생리 및 생화학적 특성과 16S rDNA에 대한 부분 염기서열 분석을 포함한 동정실험을 수행한 결과, 장내 점착능 우수균주로 선별된 L. species FSB-1은 Lactobacillus brevis로 최종 동정되었다.

Leaf-specific pathogenesis-related 10 homolog, PgPR-10.3, shows in silico binding affinity with several biologically important molecules

  • Han, Jin Haeng;Lee, Jin Hee;Lee, Ok Ran
    • Journal of Ginseng Research
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    • 제39권4호
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    • pp.406-413
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    • 2015
  • Background: Pathogenesis-related 10 (PR-10) proteins are small, cytosolic proteins with a similar three-dimensional structure. Crystal structures for several PR-10 homologs have similar overall folding patterns, with an unusually large internal cavity that is a binding site for biologically important molecules. Although structural information on PR-10 proteins is substantial, understanding of their biological function remains limited. Here, we showed that one of the PgPR-10 homologs, PgPR-10.3, shares binding properties with flavonoids, kinetin, emodin, deoxycholic acid, and ginsenoside Re (1 of the steroid glycosides). Methods: Gene expression patterns of PgPR-10.3 were analyzed by quantitative real-time PCR. The three-dimensional structure of PgPR-10 proteins was visualized by homology modeling, and docking to retrieve biologically active molecules was performed using AutoDock4 program. Results: Transcript levels of PgPR-10.3 expressed in leaves, stems, and roots of 3-wk-old ginseng plantlets were on average 86-fold lower than those of PgPR-10.2. In mature 2-yr-old ginseng plants, the mRNA of PgPR-10.3 is restricted to leaves. Ginsenoside Re production is especially prominent in leaves of Panax ginseng Meyer, and the binding property of PgPR-10.3 with ginsenoside Re suggests that this protein has an important role in the control of secondary metabolism. Conclusion: Although ginseng PR-10.3 gene is expressed in all organs of 3-wk-old plantlets, its expression is restricted to leaves in mature 2-yr-old ginseng plants. The putative binding property of PgPR-10.3 with Re is intriguing. Further verification of binding affinity with other biologically important molecules in the large hydrophobic cavity of PgPR-10.3 may provide an insight into the biological features of PR-10 proteins.

밀도 함수를 이용한 지르코니움, 바나듐, 철과 수소와의 반응성 연구 (The Hydrogen Binding Property Study by Density Functional Theory for Zr, V, Fe and Al)

  • 박태성;이택홍
    • 한국수소및신에너지학회논문집
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    • 제25권6호
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    • pp.602-608
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    • 2014
  • The sequence of bond overlap population of metal hydrogen binding is in Al-H > Fe-H > Zr-H > V-H. This results shows the binding energy of Al-H is the biggest in this metals (Al, Fe, Zr, and V) and hydrogen interaction. The Vanadium-hydrogen binding shows the weakest binding energy compared to other metals and it causes easy hydrogen desorption from the corresponding metals. The net charge of Al-H show the biggest value of 0.2248 and the severe localizations of electrons around aluminum and imply strongest covalent binding nature in these metals. This study is applicable to the purification of hydrogen in other bulk gas.

Temperature dependent hydrogen exchange study of DNA duplexes containing binding sites for Arabidopsis TCP transcription factors

  • Kim, Hee-Eun;Choi, Yong-Geun;Lee, Ae-Ree;Seo, Yeo-Jin;Kwon, Mun-Young;Lee, Joon-Hwa
    • 한국자기공명학회논문지
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    • 제18권2호
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    • pp.52-57
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    • 2014
  • The TCP domain is a DNA-binding domain present in plant transcription factors and plays important roles in various biological functions. The hydrogen exchange rate constants of the imino protons were determined for the three DNA duplexes containing the DNA-binding sites for the TCP11, TCP15, and TCP20 transcription factors using NMR spectroscopy. The M11 duplex displays unique hydrogen exchange property of the five base pairs in the first binding site (5'-GTGGG-3'). However, the M15 and M20 duplexes lead to clear changes in thermal stabilities of these five base pairs. The unique dynamic features of the five base pairs in the first binding site might play crucial roles in the sequence-specific DNA binding of the class I TCP transcription factors.

광역 객체 컴퓨팅에서 통합(이름/속성) 기반의 동적 바인딩 서비스 모델의 실험분석 (The Experimental Analysis of Integrated (Name/Property) Dynamic Binding Service Model for Wide-Area Objects Computing)

  • 정창원;주수종
    • 한국정보과학회논문지:시스템및이론
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    • 제33권10호
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    • pp.746-758
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    • 2006
  • 광역 환경에서 존재하는 수많은 서버객체들은 이름이나 속성에 의해, 다양한 중복된 성질을 갖는다. 이러한 같은 성질을 갖는 서버객체들인 중복객체들에게 서비스를 요청할 때, 기존의 네이밍이나 트레이딩 서비스는 중복된 서버객체들의 바인딩 서비스가 불가능하다. 따라서 우리는 광역 컴퓨팅 환경에서 중복객체의 선정 및 동적 바인딩 서비스를 위한 통합모델을 제시하였다. 본 모델은 중복된 객체들의 일치관리 기능뿐만 아니라 시스템들간의 부하 균형화를 유지하기 위해서 최소부하를 갖는 시스템상의 객체를 선정하는 동적 바인딩 서비스 기능을 제공한다. 이러한 목적에서 우리는 광역 컴퓨팅 환경에서 중복특성을 가진 서버객체들의 바인딩을 지원하기 위한 서비스 방안과 모델을 구축해 왔다. 본 논문에서는 구축된 모델에 대해 실험환경을 보이고, 연합 모델에서 클라이언트와 서버와의 바인딩 과정을 성능 평가하였고, 부하균형이 우리의 모델에 적용될 수 있는지 확인하기 위하여 주어진 조건을 이용하여 우리의 모델을 검증하였다. 또한 우리는 광역환경을 위한 도메인들간의 연합을 고려한 모델의 수행결과도 분석하였다. 이들 수행 결과를 통해 기존 네트워크의 물리적인 트리 구조상에서 검색 비용이 적음을 보였다.

라덱스의 물성이 도공지 품질에 미치는 상관성에 대한 연구 (Studies on the Correlation between Coated Paper and Physical Properties of Latices)

  • 박동국;조교동;고문찬;윤재한;이용규
    • 펄프종이기술
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    • 제34권1호
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    • pp.22-29
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    • 2002
  • The coated paper was greatly affected by the basic physical properties of the binder as well as the amount of the coating formula. High glass transition temperature (Tg) of the styrene-butadiene (SB) latex, selected as the binder in our study, gave the high stiffness to the coated paper, but lowered the binding force and print gloss. The average particle size of the SB latex also greatly affected to the coated paper so that the smaller particle size improved the rheological property of the coating formula and increased the binding force and print gloss. Another property of the SBR latex, gel content, was important because when its value was small, the latex was easily deformed at the high temperature and increased air permeability to the coated paper. Therefore, the lower gel content consequently resulted in the higher blistering resistance, especially in the web paper. The larger portion of the SB latex in the coated formula improved the binding force and print gloss, but decreased the ink set-off and ink-trapping to the coated paper. The heavier coating improved optical properties such as opacity, paper gloss and paper smoothness, to the coated paper.

Staphylococcus aureus의 재조합 fibronectin-binding protein의 생산 (Production of the recombinant fibronectin-binding protein of Staphylococcus aureus)

  • 김두;정자룡;박희명;한홍율
    • 대한수의학회지
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    • 제37권4호
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    • pp.875-882
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    • 1997
  • To produce the recombinant fibronectin-binding protein(FnBP) for development of subunit vaccine against Staphylococcus aureus. The fnbp gene was amplified from the chromosomal DNA of S aureus KNU 196 strain using the polymerase chain reaction, and cloned into pGEX-4T-2. Then, the recombinant FnBP fused with glutathione-S-transferase was produced in E coli, purified by affinity chromatography, and identified its antigenicity and immunogenicity by Western blot. The recombinant FnBP produced in this study is considered to have the same property of native FnBP purified from S aureus, and is expected to be useful as a candidate for S aureus subunit vaccine.

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