• 생명과학회지
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• 제5권1호
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• pp.26-32
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• 1995

The antimutagenic effects of insoluble dietary fibers(IDF) extracted from some green-yellow vegetables(kale, carrot, spinach, broccoli and soybean sprout) and soybean by binding the carcinogens of MeIQ (2-amino-3,4- dimethyl-imidazo(4,5-f) quinoline) and Trp-P-2(3-amino-1-methyl-5H-pyrido[4,3-b] indole) in Salmonella tylhimirium TA100 and TA98 were studied. All of the insoluble dietary fiber samples which binded MeIQ exhibited high antimutagenic effects by removing the mutagen. Among the samples, IDFs from kale and soybean showed strong binding capacity fo the carcinogen and revealed about 90% of the antimutagenic activity. the IDF samples showed somewhat lower binding capacity to the Trp-p-2. The lignin which extracted from kale, soybean and carrot, and the cellulose strongly removed the mutagenicity of MeIQ by the binding. Among the samples, the level of lignin in kale revealed the highest(about 10%), and it seemed that the higher content of lignin in kale is one of the reasons to increase its antimutagenic effect.

• Food Science and Biotechnology
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• 제14권6호
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• pp.866-870
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• 2005

The 14 lactic acid bacteria (LAB) have been evaluated to determine the binding capacity to HT29 cell and Aflatoxin $B_1$ ($AFB_1$). The interaction of LAB to HT29 cells has been further investigated to identify the possibility of competing the binding sites with $AFB_1$. Of 14 LAB strains, Lactobacillus casei KCTC 3260 demonstrated the higher adhesiveness to HT29 and $AFB_1$ with the rate of 19.6% and 46.3%, respectively. In competitive analysis for binding sites, the adhesion of L. casei KCTC 3260 to HT29 cells was reduced with 100 nmol $AFB_1$ by 31.2%. The protoplast of L. casei KCTC 3260 showed no binding capacity to HT29 cells with increment of $AFB_1$ concentration, indicating that cell wall components might serve as a critical factor for the binding. To discriminate the major component influencing on L. casei KCTC 3260 binding to HT29 cells and $AFB_1$, four different pre-treatments (lipase, pronase E, sodium m-periodate, and urea) were employed. Of those, sodium m-periodate treatment caused the lower adhesion of L. casei KCTC 3260 to HT29 cells with the increment of $AFB_1$ concentration. These results indicated that carbohydrate moiety on the cell wall of L. casei KCTC 3260 might be the most critical component in binding to both HT29 cells and $AFB_1$.

• 한국수산과학회지
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• 제30권1호
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• pp.31-38
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• 1997

추출조건에 따른 다시마 alginate의 성질을 조사하기 위하여 추출용매인 $Na_{2}CO_{3}$의 농도를 1, 3, 및 $5\%$로 각각 조절하고 추출시간을 각각 1, 3, 5 및 10시간으로 하였을때 각 추출조건에서 추출된 alginate의 bile acids 결합능과 물리화학적 특성을 조사한 결과는 다음과 같다. 건조다시마를 미세하게 마쇄할수록, $Na_{2}CO_{3}$ 농도와 추출시간이 증가할수록 alginate 추출량은 증가하였다. alginate의 bile acids와의 결합능은 저 농도의 $Na_{2}CO_{3}$ 용액으로 단시간에 추출한 것일수록 높게 나타났다. alginate에 의한 bile acids 결합능은 cholic acid에서 가장 높게 나타났고, taurocholic acid, glycocholic acid, deoxycholic acid 순으로 조사되었다. 다시마로 부터 alginate를 추출하고 남은 잔사(RAEA)의 bile acids와의 결합능은 alginate의 경우와 같이 추출 용액의 농도가 높을수록 추출시간이 길어질수록 감소하는 경향을 보였다. Bile acids와의 결합능은 cholic acid, taurocholic acid의 경우 alginate보다 다소 낮은 값을 나타냈고, glycocholic acid의 경우 alginate와 비슷한 값을 보였으나 deoxycholic acid와의 결합능은 추출된 alginate보다도 오히려 높은 값을 보였다. 추출된 alginate의 점도와 중합도는 추출용액의 농도가 증가하고 추출시간이 길어짐에 따라 감소하였으며 이에 비례하여 bile acid와의 결합능도 감소하였다.

• Preventive Nutrition and Food Science
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• 제15권4호
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• pp.282-286
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• 2010

To isolate a calcium-binding peptide from chlorella protein hydrolysates, chlorella protein was extracted and hydrolyzed using Flavourzyme, a commercial protease. The degree of hydrolysis and calcium-binding capacity were determined using trinitrobenzenesulfonic acid and orthophenanthroline methods, respectively. The enzymatic hydrolysis of chlorella protein for 6 hr was sufficient for the preparation of chlorella protein hydrolysates. The hydrolysates of chlorella protein were then ultra-filtered under 5 kDa as molecular weight. The membrane-filtered solution was fractionated using ion exchange, reverse phase, normal phase chromatography, and fast protein liquid chromatography to identify a calcium-binding peptide. The purified calcium-binding peptide had a calcium binding activity of 0.166 mM and was determined to be 700.48 Da as molecular weight, and partially identified as a peptide containing Asn-Ser-Gly-Cys based on liquid chromatography/electrospray ionization tandem mass spectrum.

• 한국식품영양과학회지
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• 제7권1호
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• pp.43-52
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• 1978

It is well known that water holding capacity plays an important role in processing such meat products as frankfurter-type sausage and fish meat paste products as kamaboko and fish sausage. Consumer qualities of meat products, such as appearance, flavor, as well as drip and shrinkage on cooking, depend greatly on the degree of water binding. In this paper, the water holding capacities of fish paste and salt added paste of white corvenia, Argyrosomus argentatus and file fish, Novodon modestus were measured by centrifuging and press method before and after cooking. And the effects of the addition of phosphates and starch to enhance water binding and stabilize gel formation were also discussed. In addition, the experimental conditions which are suitable to determine the water binding of fish meat paste product were suggested. The results were expressed in percent of water absorbed by the filter paper when pressed or released by pressor or centrifuge to the weight of sample. From the results. a proper condition to measure the water holding capacity of fish meat paste was that 3.0 g of sample which was previously added with 10 percent water was centrifuged at 13,400 G or 12,000 rpm for 15 minutes for the centriguging method and for press method, 0.3 g sample with 10 percent of water added was extracted by an oil pressor at $30\;kg/cm^2$ for 1 minute. Water holding capacity of fresh paste of white corvenia was relatively higher than that of file fish and the difference between species of fish was greater than the difference between measurments by two methods. Sodium chloride had a great effect on enhancing the water holding capacity of fish meat paste giving better effect when 3.0 percent of salt was added. Phosphates used except calcium phosphate revealed a certain enhancement in water binding, yielding best effect at 0.3 percent addition, and metaphosphate seemed to be more effective in order. The addition of corn starch, however, appeared to be not so effective for enhancement of water binding in fresh-salt-added fish meat paste but in cooked fish paste which might be attributed to absorption of water by starch grain and swelling during the heating and consequently enforced gel strength of cooked fish paste. And the water holding capacity of cooked fish paste was proportionally related to its gel strength.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 1995년도 봄 학술발표회 논문집
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• pp.106-110
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• 1995

Chloride ion is considered one of the most common culprits in the corrosion of steel in concrete. It breaks down the passive film and allows the steel to corrode actively at a high rate. The main objective of this study is to determine the critical chloride ion concentrations in the pore solutions and chloride binding effect of cement pastes made with and without fly ash. Cement pastes with water-ratio of 0.5 allowed to hydrate in sealed containers for 28 days and to express poresolution. T도 expressed pore fluids were analyzed for chloride and hydroxyl ion concentrations. It was found that the replaced cement with fly ash have little effect on Chloride binding capacity ratio.

• Asian-Australasian Journal of Animal Sciences
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• 제3권1호
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• pp.53-59
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• 1990

A study was conducted to investigate the release pattern of zinc form the zinc containing boluses and to see whether the released zinc can cure a zinc deficiency in sheep. Three sheep were used in this experiment and were fed a low zinc semi-synthetic diet throughout the experimental period. Each sheep was given a single pre-weighed zinc containing bolus when blood variables showed continuous zinc deficiency. The zinc containing boluses when placed within the reticulo-rumen of zinc deficient sheep, release zinc at the rate of 106.6 mg zinc/day for 39 days. At the end of depletion period there was a reduced feed consumption, plasma zinc concentration, plasma alkaline phosphatase activity and increased plasma zinc binding capacity which were 409 g, 0.18 mg/l, 87 U/l and 88.7% respectively and 521 g, 0.18 mg/l, 142 U/l, and 89.5% respectively before first and second blousing. After the administration of the first and second boluses, the feed consumption, plasma zinc levels and plasma alkaline phosphatase activities rose rapidly and far exceeded the starting values. The zinc binding capacity was reduced to 21.9% due to the administration of the first and second boluses. It is concluded that zinc boluses can be used for curing a zinc deficiency in sheep.

• 한국식품조리과학회지
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• 제8권3호
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• pp.333-341
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• 1992

The physicochemical, rheological and sensory characteristics of 'BACKSULGIES', which was added with potato peel, guar gum or polydextrose, were investigated. The maximum acceptable addition ratio of dietary fiber to 'BACKSULGI' was 10%. And optimal addition ratio was 3% for all samples. The water binding capacity was affected by dietary fiber sources and incubation conditions (temperature and time). The Guar gum had me highest value of water binding capacity. The solubility was highly related with water binding capacity and me swelling power was increased with temperature increment. The degree of gelatinization was not significantly different with dietary fiber sources. But me values of gelatinization of 'BACKSULGIES' added with dietary fibers were significantly higher than mose of 'BACKSULGI' with no dietary fiber. Generally hardness and brittleness incresed along with storage time. But me hardness of 'BACKSULGIES' added with dietary fibers was significantly lower man those of 'BACKSULGI' with no dietary fiber. The retardation effect of dietary fibers for retrogradation of 'BACKSULGIES' was also proved by time constant determination of Avrami equation. Sernsory evaluation revealed that me addition of dietary fibers did not reduce the organoreptic quality. Therefore potato peel 3%, guar gum 3%, polydextrose 3% were optimum addition ratio which could be accepted as conventional 'BACKSULGI'. As me results of this study, it was proved mat the additions of dietary fibers to 'BACKSULGI' had the retardation effect of retrogradation.

• 대한핵의학회:학술대회논문집
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• 대한핵의학회 1973년도 제12차 학술대회
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• pp.52.1-52.1
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• 1973

• Archives of Pharmacal Research
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• 제13권4호
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• pp.355-358
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• 1990

In order to investigate the cellular mechanisms of hypoglycemic of brazilin, hepatocyte monolayer culture was introduced and, glycogen synthesis rate and insulin binding were measured as parameters. Glycogen synthesis and insulin sensitivity were remarkably augmented by the treatment of brazilin. Brazilin slightely increased insulin binding. Scatchard analysis revealed that this increase in insulin binding was not due to increase in the binding capacity but in binding affinity. These results suggest that the augmentation of hepatic glycogenesis and insulin sensitivity by brazilin may play an important role in the improvement of hyperglycemia.