Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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The Binding of Aflatoxin $B_1$ Modulates the Adhesion Properties of Lactobacillus casei KCTC 3260 to a HT29 Colon Cancer Cell Line

  • Hwang, Kwon-Tack (Biofood Research Center, Chonnam National University) ;
  • Lee, Won-Jae (Department of Food Science, University of Wisconsin-Madison) ;
  • Kim, Gye-Yeop (Department of Exercise Prescription, Dongshin University) ;
  • Lee, Shin-Kyung (Department of Food and Nutrition, Nambu University) ;
  • Lee, Jeong-Min (Department of Food and Nutrition, Nambu University) ;
  • Jun, Woo-Jin (Department of Food and Nutrition, Chonnam National University)
  • Published : 2005.12.31
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Abstract

The 14 lactic acid bacteria (LAB) have been evaluated to determine the binding capacity to HT29 cell and Aflatoxin $B_1$ ($AFB_1$). The interaction of LAB to HT29 cells has been further investigated to identify the possibility of competing the binding sites with $AFB_1$. Of 14 LAB strains, Lactobacillus casei KCTC 3260 demonstrated the higher adhesiveness to HT29 and $AFB_1$ with the rate of 19.6% and 46.3%, respectively. In competitive analysis for binding sites, the adhesion of L. casei KCTC 3260 to HT29 cells was reduced with 100 nmol $AFB_1$ by 31.2%. The protoplast of L. casei KCTC 3260 showed no binding capacity to HT29 cells with increment of $AFB_1$ concentration, indicating that cell wall components might serve as a critical factor for the binding. To discriminate the major component influencing on L. casei KCTC 3260 binding to HT29 cells and $AFB_1$, four different pre-treatments (lipase, pronase E, sodium m-periodate, and urea) were employed. Of those, sodium m-periodate treatment caused the lower adhesion of L. casei KCTC 3260 to HT29 cells with the increment of $AFB_1$ concentration. These results indicated that carbohydrate moiety on the cell wall of L. casei KCTC 3260 might be the most critical component in binding to both HT29 cells and $AFB_1$.

Keywords

References

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