• 한국식품조리과학회지
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• 제22권3호통권93호
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• pp.270-281
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• 2006

The study investigated the effect of prefermentation of Bifidobacteria longum and Lactobacillus plantarum on baking quality. Firstly, two kinds of prefermentation were cultured using two lactic acid bacteria, Bifidobacteria longum and Lactobacillus plantarum. White pan bread baked with dough that had undergone these two prefermentation methods was compared with that baked with a non-prefermented control. The physicochemical properties of the three breads were analyzed, and then the physicochemical and sensory properties of the dough and baked bread were cross-analyzed. The pH prefermentation of Bifidobacteria longum was lower than that of Lactobacillus plantarum, whereas the titratable acidity was higher. Compared to the results from analyzing the prefermentation of Lactobacillus plantarum, the prefermentation of Bifidobacteria longum was expected to give positive effects on enriching the bread flavor by creating acetic acid at a level three- to eight-fold higher than that of Lactobacillus plantarum. According to the mixogram data, the optimum ending time for both Bifidobacteria longum prefermentation and Lactobacillus plantarum was around 4.5 to 5 minutes. The speed of dough materialization decreased with increasing prefermentation culture time. The baked bread with added Bifidobacteria longum had a higher water content. However, the other contents were not influenced by prefermentation, but were by culture time. The specific loaf volume, oven spring and baking lass rate all peaked at 20 hours after culture for both prefermentation cultures. The sensory test results indicated the highest prefermentation for the bread baked with prefermented Bifidobacteria longum doughwith a culture time ranging from 20 to 26 hours. In addition, the bread baked with prefermented Lactobacillus plantarum dough gave the highest preference when cultured for 20 hours.

• 한국식품과학회지
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• 제38권2호
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• pp.232-236
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• 2006

건강한 성인의 분변에서 분리한 내산성 및 내담즙성이 우수한 B. longum BO-11을 김치 제조용 스타터로 이용하여 그 생존 능력과 김치 발효에 미치는 영향에 대해 조사하였다. 발효과정 중 bifidobacteria의 계수는 BS 배지를 사용하였으며 20일의 저장 기간 동안 균수가 약간 증가하였다. 일반 미생물은 bifidobacteria 비첨가군과 비슷하였으며 bifidobacteria 첨가군의 경우 산의 생산이 더 많았다. 김치 맛은 일반 김치보다 bifidobacteria 첨가 김치가 더 우수하였다. 이러한 결과는 bifidobacteria를 이용한 기능성 김치의 상업화 가능성을 보여준다.

• Journal of Dairy Science and Biotechnology
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• 제33권2호
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• pp.103-110
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• 2015

산양유을 섭취한 성인의 분변 내 bifidobacteria의 증식 효과 및 분변에서 분리된 bifidobacteria와 lactobacilli의 당 발효 능력을 조사하였다. 1. 산양유를 섭취한 처리군이 대조군보다 bifidobacteria 세균수가 상대적으로 높았으며, 8주에서 95% 신뢰한계에서 유의성이 있었다. 2. 분변에서 분리한 균주들을 13 균주의 16S rRNA 염기서 열로 동정한 결과, 산양유를 섭취한 처리구는 B. adolescentis, B. longum, B. pseudocatenulatum, B. dentium, L. sakei 순으로 분리되었다. 대조군의 12 균주는 B. adolescentis, B. longum, L. ruminis, L. sakei, B. pseudocatenulatum 순으로 분리되었다. 3. 산양유 올리고당과 lactulose는 모든 bifidobacteria 및 lactobacilli 균주가 발효하였다. Fructooligosaccaride는 B. adolescentis 7 균주 모두 발효하였다. 조사한 B. pseudocatenulatum 4 균주, L. sakei 3 균주, B. longum 7 균주 중에서 각각 3, 2, 1 균주가 fructooligosaccharide를 발효하였다.

• 한국축산식품학회지
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• 제26권4호
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• pp.497-502
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• 2006

한국인 유아의 분변에서 분리한 10종의 분리균주를 포함하여 총 16종의 bifidobacteria에 대한 Caco-2 세포에 대한 부착성과 세포 표면 소수성을 측정하였다. 부착성과 세포 표면소수성 모두 균주에 따라 상이한 결과를 나타내었으며 균종에 따른 경향은 관찰되지 않았다. 실험 균주 중에서 B. longum D6, B. longum H4, B. breve Ml, B. thermophilum ATCC 25525, B. suis ATCC 27533, B. animalis subsp. lactis BB12 균주가 세포 표면 소수성이 높게 나타났으며, Caco-2 세포에 대한 부착성은 B. bifidum B3, B. longum D6, B. longum H4, B. thermophilum ATCC 25525, B. suis ATCC 27533, B. animalis subsp. lactis BB12, B. longum 2 등이 우수하였다. 또한 Caco-2세포에 200개 이상이 부착되는 실험 균주는 모두 60% 이상의 세포 표면 소수성을 나타내고 있으므로, 부착성이 우수한 균주 선발 시 분리 균주의 세포 표면 소수성을 측정하여 선발하는 것이 가능하다고 판단되었다.

• 한국식품위생안전성학회지
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• 제12권3호
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• pp.181-187
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• 1997

Lactobacillus acidophilus NCFM, Lactobacillus casei YIT 9018, Bifidobacterium longum 8001, and Bifidobacterium longum 8025 at the level of 106 cfu/$m\ell$ were cultured with 104 cfu/$m\ell$ of Escherichia coli O157:H7 KSC 109 or Salmonella typhimurium ATCC14028, in order to verify the effects of lactic acid bacteria and bifidobacteria on the growth of the pathogens. In the mixed culture of lactic acid bacteria with E. coli O157:H7 KSC 109, Growth inhibition and atypical microcolonies of E. coli O157:H7 KSC 109 were observed. The pathogens inoculated grew for 5 hors (pH 5.3), by the time L. acidophilus NCFM reached the exponential growth phase, and then the surviving pathogens were decreased to 101 cfu/$m\ell$ after 35 hours. When L. caseiYIT 9018 was grown with the pathogens, they grew for 10 hours (pH 4.6), by the time L. casei YIT 9018 reached the end of exponential growth phase, and then the surviving pathogens were decreased drastically. Up to the stationary growth phase of lactic acid bacteria, L. acidophilus NCFM exhibited stronger inhibition against the pathogens than L. casei YIT 9018 did, which might be attributed to its faster growth. Likewise bifidobacteria inhibited the growth of the pathogens tested, bifidobaceria was weaker in the inhibitory activity than lactic acid bacteria. When Bifidobacterium longum 8001 was cultured with the pathogens, E. coli O157:H7 KSC 109 was gradually ingibited at the stationary growth phase of bifidobacteria, atypical microcolonies were formed on Levine EMB medium after 48 hours, and Salmonella grew up to 106 dfu/$m\ell$, then was drastically ingibited at the exponential growth phage of Bifidobacterium longum 8001. But when Bifidobacteriuam longum 8025 was cultured with the pathogens, the pathogens grew to the same level of Bifidobacteriuam longum 8025 was cultured with the pathogens, the pathogens grew to the same lever of Bifidobacteriuam longum 8025 after 10 hours, then the surviving pathogens were decreased drastically.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.203-206
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• 2000

Bifidobacteria의 배양효율을 증대하기 위하여 완충제로써 $CaCO_3$를 Ca-alginate에 고정화한 비드를 제조하여 사용하였다. B. longum KCTC 3218과 한국인 분변에서 분리한 B. longum HLC 3742 균주를 $CaCO_3$ 비드, NaOH, $Na_2CO_3$, $NH_4OH$ 등을 완충제로 각각 사용한 2.5-liter 발효기에서 각각 배양하여 균 증식과 저장에 따른 생존력을 조사한 결과 $CaCO_3$ 비드를 완충제로 사용한 경우가 다른 완충제를 사용한 경우보다 균 증식과 저장 안정성에서 효과적임을 알 수 있었다. 따라서 완충제로써 $CaCO_3$ 비드는 bifidobacteria의 고농도 배양과 생존력 증대에 유용할 것으로 사료되었다.

• Journal of Microbiology and Biotechnology
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• 제10권2호
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• pp.147-153
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• 2000

This study was conducted to compare probiotic activities and physiological functions of Bifidobacterium longum Mk-G7 with weveral commercial and type strains of bifidobacteria. bif. longum MK-G7 showed the highest acid tolerance against HCl and acetic acid, whereas bif. infantis Y-1 showed the lowest acid tolerance and more than 4 log cycles of viable cell count decreased due to acid injuty. Viable cell counts of bifidobacteria strains decreased more than 1.5 log cycles owing to oxygen toxicity, with the exception of Bif. longum MK-G7, Bif. infantis Y-2, Bif. longum Y-3, Bif. longum Y-6, and Bif. longum RD-13 showed the highest bile tolerance, whereas Bif. longum MK-G7 showed a medium level of bile tolerance. Only Bif. longum MK-G7 howed much higher antibiotic resistance against both tetracycline and penicillin-G in the MIC(minimum inhibitory concentration) level of 24.8 mg/I and 0.52mg/I, respectively. Bif longum Y-6, and Bif. bifidum ATCC 29539 showed more than 80% of anti-mutagenicity against NQO(4-nitroquinolinel-oxide). Since the production of cytokines such as $TNF(tumor necrosis factor)-{\alpha}$ and IL (interleukin)-6, and NO(nitric oxide) in the macrophage cell line Raw 264.7 cells increased as Bif. longum MK-G7 cell concentration increased, ti was suggested that Bif. longum MK-G7 is able to enhance immunopotentiating activity in vitro. When freeze-dred Bif. longum MK-G7 was administered to mice at the dose of 1,2,4, and 6 g/kg of body weight, all of the mice survived in all feeding groups, proving the GRAS(generally recognized as safe) status of Bif. longum MK-G7. When fermented milk containing Bif. longum MK-G7 was administered to human volunteers, viable cell count of total bifidobacteria and anaerobes in the feces increased up to 0.5 log cycles more than before the administration. In particular, Bif. logum MK-G7 ingibited the growth of Bacteroides at the level of 1.0-1.5 log cycles.

• Journal of Microbiology and Biotechnology
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• 제11권3호
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• pp.443-451
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• 2001

In order to investigate what kind of response anaerobic bifidobacteria has on oxygen stress, five oxygen-tolerant bifidobacteria were isolated from human fecal samples. All were temporarily identified as Bifidobacterium longum through an analysis of carbohydrate utilization patterns and cellular fatty acid profiles. In the presence of oxygen, the lag phase became extended and the cell growth was suppressed. Bifidobacterial cell was able to remove dissolved oxygen in an early stage of growth and to overcome oxygen stress to a certain extent. The cell became long n size and showed a rough surface containing many nodes which were derived from abnormal or incomplete cell division. Cellular fatty acid profiled changed remarkably under a partially aerobic condition, so that the carbon chain of cellular fatty acid became short. All the dimethyl acetals originated from plasmalogen were reduced, any cyclopropane fatty acid, 9, 10-methyleneoctadecanoic acid ($C_{19:0}cyc9,10$), was increased remarkably. Oxygen stress induced a 5.5 kD protein in B. longum JI 1 of the oxygen-teolerant bifidobacteria, that was named Osp protein, and its N-terminal amino acid sequence was as follows: unknown amino acid-Thr-Gly-Val-Arg-Phe-Ser-Asp-Asp-Glu. Therefore, the oxygen-tolerant bifidobacteria seemed to defend against oxygen stress byincreasing the content of short fatty acid and cyclopropane fatty acid, and induction of an oxygen stress protein, but not the plasmalogen.

• Journal of Microbiology and Biotechnology
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• 제10권2호
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• pp.143-146
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• 2000

This study was conducted to compare the cellular fatty acid composition and genotypic analysis of Bifidobacterium longum MK-G7 originated from Koreans with other commercial type strains of bifidobacteria. The cellular fatty acid of Bif. longum MK-G7 was shown to be composed of $C_{160FAME},C_{181\;c18DMA},C_{18.1\;CIS9\; FAME},C_{14.0FAME},C_{19\;0cye9,10 DMA},Feature7(C_{17.2 FAME), and Feature 10(C_{181\; Cll/t9/t6 FAME}$. Bif. longum MK-G7 showed 99.9% homology and the highest relatedness with Bif. longum ATCC 15707 type strain. Both Bif. longum MK-G7 and Bif. longum ATCC 15707 showed 153 bp products on RAPD (randomly amplified polymorphic DNA) analysis, however, they showed quite different band patterns on PFGE (pulsed-field gel electrophoresis) analysis. Consequently, our present study showed that Bif. longum MK-G7 was different from any commercial type strains of Bif. longum tested.

• 한국미생물·생명공학회지
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• 제26권6호
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• pp.476-482
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• 1998

Bifidobacterium의 효과적인 산업적 이용을 위해서는 산소에 내성이 강한 균주를 선발하는 연구뿐만 아니라, 산소 존재하에서 비피도박테리아의 내성기작을 규명하는 기초적인 연구도 필요하다. 산소에 민감한 B. adolescentis 균주와 산소내성 B. longum JI-1균주의 용존산소 제거능을 비교한 결과산소 민감성 균주는 총존산소를 전혀 제거하지 못하였으나 산소내성 균주는 10분 이내에 3%이상의 용존산소를 제거하여 총존산소 제거능과 Bifidobacterium의 산소내성과는 밀접한 관계가 있음을 알 수 있었다. 이 B. longum JI-1 균주를 미호기성 조건에서 배양하여 내산소성 돌연변이 균주인 B. longum ADJ-1 균주를 분리하였다. B. longum ADJ-1과 모균주의 특성을 당자화능, NADH 산화효소 및 세포지방산등으로 비교하였을 때 차이점을 발견하지 못했으며, 균생육은 B. longum ADJ-1이 모균주의 80% 정도를 보여 주었다. 그러나 B. longum ADJ-1는 매우 두꺼운 slime layer를 형성하였는데 confocal scanning laser microscopy에 의한 분석 결과 돌연변이 균주는직경이 약 6 $\mu\textrm{m}$에 이르는 층을 형성한 반면 모균주는 약 3 $\mu\textrm{m}$인 층을 형성하였다. 그리고 돌연변이 균주는 산소에 24시간 이상 노출되었을 경우 모균주에 비하여 더 큰 산소내성을 보여주었다. 그러므로 돌연변이 균주의 산소내성의 증가는 slime layer 차이에서 유래된 것으로 생각되며 이는 Bifidobacterium의 내산소성 기작중의 하나인 것으로 보인다.