• Bulletin of the Korean Chemical Society
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• v.15 no.5
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• pp.394-399
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• 1994

The catalytic mechanism of malonyl-CoA synthetase from Rhizobium trifolii was investigated by the steady state kinetics and intermediate identification. Initial velocity studies and the product inhibition studies with AMP and PPi strongly suggested ordered Bi Uni Uni Bi Ping-Pong Ter Ter system as the most probable steady state kinetic mechanism of malonyl-CoA synthetase. Michaelis constants were $0.17{\pm}0.04 {\mu}M,\;0.24{\pm}0.18 {\mu}M\;and\;0.045{\pm}0.26 {\mu}$M for ATP, malonate and CoA, respectively. The TLC analysis of the $^{32}P-labelled$ products in reaction mixture containing $[{\gamma}-^{32}P]$ ATP in the absence of CoA showed that PPi was produced after the sequential addition of ATP and malonate. Formation of malonyl-AMP, suggested as an intermediate in the kinetically deduced mechanism, was confirmed by the analysis of $^{31}P-NMR$ spectra of AMP product isolated from the $^{18}O$ transfer experiment using $[^{18}O]$malonate. Two resonances were observed, corresponding to AMP labelled with zero and one atom of $^{18}O$, indicating that one atom of $^{18}O$ transferred from $[^{18}O]$malonate to AMP through the formation of malonyl-AMP. Formation of malonyl-AMP was also confirmed through the TLC analysis of reaction mixture containing $[{\alpha}-^{32}P]$ATP. These results strongly support the ordered Bi Uni Uni Bi Ping-Pong Ter Ter mechanism deduced from the initial velocity and product inhibition studies.

• Proceedings of the Korean Society of Toxicology Conference
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• 2001.10a
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• pp.37-38
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• 2001

The cytochrome P450 (P450) 1 family (1A1, 1A2, 1B1) is involved in the activation of many pro-carcinogens. Previously we characterized a number of synthetic bi- and polycyclic hydrocarbon acetylenes as selective-mechanism-based inhibitors of recombinent P450s 1A1, 1A2, 1B1 (Shimada et al., Chem. Res. Toxicol., 11, 1048-1056, 1998). We reported that the drug oltipraz is a mechanism-based indicator of P450 1A2 (Lagouet et al. Chem. Res. Toxicol., 13, 245-252, 2000).(omitted)

• Korean Journal of Remote Sensing
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• v.30 no.3
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• pp.367-373
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• 2014

Albedo is one of the critical parameters for understanding global climate change and energy/water balance. In this study, we used red and NIR reflectance from Satellite Pour I'Obervation de la Terre (SPOT)/Vegetation (VGT) S1 product. The product is preprocessed for users that they are atmospherically corrected using Simple Method Atmospheric Correction (SMAC) by Vision on Technology (VITO) for calculating broadband albedo. Roujean's Bi-directional Reflectance Distribution Function (BRDF) model is a semi-empirical method used for BRDF angular integration and inversion. Each kernel of Roujean's model was multi integrated by angle components (i.e., viewing zenith, solar zenith, and relative azimuth angle). Black-sky hemispherical function is integrated by observational angle; whereas, white-sky hemispherical efficient is integrated by incident angle. Estimated spectral albedo of red ($0.61{\sim}0.68{\mu}m$, B2) and near infrared ($0.79{\sim}0.89{\mu}m$, B3) have a good agreement with MODIS albedo products.

• Transactions of the Korean hydrogen and new energy society
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• v.33 no.6
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• pp.636-642
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• 2022

The "Hydrogen Economic Activation Road map" was announced in 2019, and hydrogen demand is expected to exceed 470,000 tons per year in 2022 and keep increasing. Under this circumstance, it has become important to understand the greenhouse gas (GHG) emissions associated with various hydrogen production pathways. In this study, the evaluation of life cycle GHG emissions regarding the hydrogen produced as by-product from coke oven gas (COG) in steel mill is conducted. To cover the possible range of operations, three literatures were reviewed and their data of inputs and outputs for the process were adopted for calculation. Life cycle inventories and emission factors were mostly referred to GaBi and Intergovernmental Panel on Climate Change (IPCC) guidelines, respectively. When there are multiple products from a single process, the energy allocation method was applied. Based on these sources and the assumptions, the life cycle emission values of COG-based hydrogen were found to be 3.8 to 4.7 kg/CO₂-eq./kg-H₂.

• Elastomers and Composites
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• v.49 no.3
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• pp.220-224
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• 2014

The tire tread is contacted with road surface directly. It gives significant effect on the breaking conditions, traction, noise and so on. The tread having grooves with complex geometry is molded by shaping process. The flow behavior of tread rubber in a mold affects the quality of the tread and it leads to the running performance of automobile. In this study, the flow behavior of rubber in shaping process has been investigated by computer simulation. The objective of flow simulation is the design of tread shape based on the contact of rubber on the mold surface and flow behavior of rubber. Different sequences of contact of rubber on the mold surface and flow behavior of rubber are observed according to the shape of tread on the mold surface. It was verified that the shape of tread gives significant effect on the flow behavior of rubber. Different flow behaviors of rubber and sequential contact of rubber to the mold surface were observed according to the shape of tread on the mold surface. Therefore, we have identified that the shape of tread give a change in the flow behavior of rubber.

• Journal of applied mathematics & informatics
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• v.4 no.1
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• pp.1-16
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• 1997

In this paper we study efficient parallel implementation for hybrid iterative methods BICGSTAB and BICGSTAB $(\ell)$ with ${Well}=2$ on the CRAY C90 and the efficiency of their parallel performance is evaluated. numerical experiments suggest that on the CRAY C90 a parallel inner product algorithm called PDOTB be used for the par-allelization of hybrid iterative methods containing sensitive values of inner products. Lastly it is shown that the number of iterations in which parallel hybrid iterative methods satisfy a certain convergence criterion depends on the number of processors to be used.

• Journal of Advanced Navigation Technology
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• v.17 no.6
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• pp.646-651
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• 2013

In this paper, a small inverted-F antenna with adjustable characteristics is proposed with use of lumped elements, capacitor and resistor. Capacitor is inserted between end of the antenna and ground for size reduction and tuning of resonant frequency. On the other hand, $0{\Omega}$ resistor is replaced as the short line connected to ground. The movement of short point due to use of $0{\Omega}$ resistor results in providing variation of input impedance. Therefore optimum characteristics can be obtained only by proper choice of capacitor and short point without redesign of it's geometry. In order to check the validity, the proposed antenna is designed and fabricated for 2.4 GHz frequency band, and then is applied to a product of Zigbee wireless remote controller. As a result, the size of applied antenna is $8.5{\times}4.5mm^2$ and it is observed that the measured bandwidth and antenna gain are 150 MHz and 2 dBi respectively without redesign of the antenna.

• Fashion & Textile Research Journal
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• v.18 no.5
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• pp.637-646
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• 2016

The goal of this study is to provide basic data on developing circular knit basic pattern for women in their 20's. Production conditions of circular knit product pattern making among domestic women's apparel industry was researched, and collected data on sizes and ease amounts from woven and circular knit pattern were compared and analyzed. According to the result of the survey, product measurements adjusted to the actual body size fit for the brand's image were used, and the common problem among manufacturers and consumers regarding circular knit products turned out to be change in size and form due to stretching. For the basic pattern of circular knit, stretching quality was reflected in the woven basic pattern based on plain stitch(single knit) and then dart was removed and ease amount was reduced. The result of looking into size and ease amount about woven and circular knit torso & sleeve block shows that there is a significant difference among chest circumference, hip circumference, bi-shoulder length, interscye back, interscye front, scye depth, upper arm circumference and wrist circumference, and it was clear that circumference and width on the areas around the wrist tended to fit around the body more when circular knit was used instead of woven fabric.

• International Journal of Oral Biology
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• v.44 no.3
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• pp.89-95
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• 2019

Piperlongumine (PL) is a natural product found in long pepper (Piper longum). The pharmacological effects of PL are well known, and it has been used for pain, hepatoprotection, and asthma in Oriental medicine. No studies have examined the effects of PL on bone tissue or bone-related diseases, including osteoporosis. The current study investigated for the first time the inhibitory effects of PL on osteoclast differentiation, bone resorption, and osteoclastogenesis-related factors in RAW264.7 macrophages stimulated by the receptor activator for nuclear factor-${\kappa}B$ ligand (RANKL). Cytotoxicity was examined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and osteoclast differentiation and bone resorption were confirmed by tartrate-resistant acid phosphatase (TRAP) staining and pit formation analysis. Osteoclast differentiation factors were confirmed by western blotting. PL exhibited toxicity in RAW264.7 macrophages, inhibiting osteoclast formation and bone resorption, in addition to inhibiting the expression of osteoclastogenesis-related factors, such as tumor necrosis factor receptor-associated factor 6 (TRAF6), c-Fos, and NFATc1, in RANKL-stimulated RAW264.7 macrophages. These findings suggest that PL is suitable for the treatment of osteoporosis, and it serves as a potential therapeutic agent for various bone diseases.

• Natural Product Sciences
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• v.11 no.3
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• pp.165-169
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• 2005

Chlorophytum borivilianum Baker (Antharicaceae) commonly referred as 'Safed Musli' has been widely used in the Indian traditional systems of medicine to treat various diseases like rheumatism apart from having immunomodulating property and is used as general tonic. It is also known as 'Ayurvedic viagra' for its aphordisiac properties. C. borivilianum was screened for the first time to determine its antioxidant activity, isolation of the sapogenins and standardization of the isolated sapogenin fraction using HPTLC. Potent antioxidant activity of ethanolic extract was found by their ability to scavenge DPPH (84.51%), hydroxyl radical (48.95 %), ferryl bi-pyridyl complex (84.53%) along with the inhibition of lipid peroxidation (67.17%) at $100\;{\mu}g/ml$ concentration. The ethanolic extract also exhibited significant inhibition of superoxide anion radical generated by photochemiluminescence. Thus, the potent antioxidant activity validates the innumerable therapeutic claims of the plant in the traditional system especially its use as a Rasayana drug.