• Bulletin of the Korean Chemical Society
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• 제15권5호
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• pp.394-399
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• 1994

The catalytic mechanism of malonyl-CoA synthetase from Rhizobium trifolii was investigated by the steady state kinetics and intermediate identification. Initial velocity studies and the product inhibition studies with AMP and PPi strongly suggested ordered Bi Uni Uni Bi Ping-Pong Ter Ter system as the most probable steady state kinetic mechanism of malonyl-CoA synthetase. Michaelis constants were $0.17{\pm}0.04 {\mu}M,\;0.24{\pm}0.18 {\mu}M\;and\;0.045{\pm}0.26 {\mu}$M for ATP, malonate and CoA, respectively. The TLC analysis of the $^{32}P-labelled$ products in reaction mixture containing $[{\gamma}-^{32}P]$ ATP in the absence of CoA showed that PPi was produced after the sequential addition of ATP and malonate. Formation of malonyl-AMP, suggested as an intermediate in the kinetically deduced mechanism, was confirmed by the analysis of $^{31}P-NMR$ spectra of AMP product isolated from the $^{18}O$ transfer experiment using $[^{18}O]$malonate. Two resonances were observed, corresponding to AMP labelled with zero and one atom of $^{18}O$, indicating that one atom of $^{18}O$ transferred from $[^{18}O]$malonate to AMP through the formation of malonyl-AMP. Formation of malonyl-AMP was also confirmed through the TLC analysis of reaction mixture containing $[{\alpha}-^{32}P]$ATP. These results strongly support the ordered Bi Uni Uni Bi Ping-Pong Ter Ter mechanism deduced from the initial velocity and product inhibition studies.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2001년도 International Symposium on Dietary and Medicinal Antimutgens and Anticarcinogens
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• pp.37-38
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• 2001

The cytochrome P450 (P450) 1 family (1A1, 1A2, 1B1) is involved in the activation of many pro-carcinogens. Previously we characterized a number of synthetic bi- and polycyclic hydrocarbon acetylenes as selective-mechanism-based inhibitors of recombinent P450s 1A1, 1A2, 1B1 (Shimada et al., Chem. Res. Toxicol., 11, 1048-1056, 1998). We reported that the drug oltipraz is a mechanism-based indicator of P450 1A2 (Lagouet et al. Chem. Res. Toxicol., 13, 245-252, 2000).(omitted)

• 대한원격탐사학회지
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• 제30권3호
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• pp.367-373
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• 2014

Albedo is one of the critical parameters for understanding global climate change and energy/water balance. In this study, we used red and NIR reflectance from Satellite Pour I'Obervation de la Terre (SPOT)/Vegetation (VGT) S1 product. The product is preprocessed for users that they are atmospherically corrected using Simple Method Atmospheric Correction (SMAC) by Vision on Technology (VITO) for calculating broadband albedo. Roujean's Bi-directional Reflectance Distribution Function (BRDF) model is a semi-empirical method used for BRDF angular integration and inversion. Each kernel of Roujean's model was multi integrated by angle components (i.e., viewing zenith, solar zenith, and relative azimuth angle). Black-sky hemispherical function is integrated by observational angle; whereas, white-sky hemispherical efficient is integrated by incident angle. Estimated spectral albedo of red ($0.61{\sim}0.68{\mu}m$, B2) and near infrared ($0.79{\sim}0.89{\mu}m$, B3) have a good agreement with MODIS albedo products.

• 한국수소및신에너지학회논문집
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• 제33권6호
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• pp.636-642
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• 2022

The "Hydrogen Economic Activation Road map" was announced in 2019, and hydrogen demand is expected to exceed 470,000 tons per year in 2022 and keep increasing. Under this circumstance, it has become important to understand the greenhouse gas (GHG) emissions associated with various hydrogen production pathways. In this study, the evaluation of life cycle GHG emissions regarding the hydrogen produced as by-product from coke oven gas (COG) in steel mill is conducted. To cover the possible range of operations, three literatures were reviewed and their data of inputs and outputs for the process were adopted for calculation. Life cycle inventories and emission factors were mostly referred to GaBi and Intergovernmental Panel on Climate Change (IPCC) guidelines, respectively. When there are multiple products from a single process, the energy allocation method was applied. Based on these sources and the assumptions, the life cycle emission values of COG-based hydrogen were found to be 3.8 to 4.7 kg/CO₂-eq./kg-H₂.

• Elastomers and Composites
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• 제49권3호
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• pp.220-224
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• 2014

자동차 타이어의 트레드는 노면과 직접적으로 접촉하며 제동력, 구동력, 소음 등 중요한 역할을 한다. 트레드는 많은 그루브로 이루어져 있는데 고무의 형상을 형성하는 가류 공정에서 불량이 발생하게 되면 트레드의 기능이 저하되어 자동차 성능의 문제로 이어지게 된다. 본 논문은 CAE 해석을 통하여 가류 공정 중 구체적인 고무의 흐름을 분석하였다. 트레드 형상에 따라 고무 흐름의 편차가 발생하기 때문에 형상에 따른 트레드의 고무가 금형에 접촉되는 순서와 속도 그리고 고무의 흐름을 관찰하였다. 트레드의 형상에 따라서 고무가 금형에 안착되는 양상이 다르게 나타났으며 고무의 흐름에 큰 변화를 주는 것을 확인할 수 있었다.

• Journal of applied mathematics & informatics
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• 제4권1호
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• pp.1-16
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• 1997

In this paper we study efficient parallel implementation for hybrid iterative methods BICGSTAB and BICGSTAB $(\ell)$ with ${Well}=2$ on the CRAY C90 and the efficiency of their parallel performance is evaluated. numerical experiments suggest that on the CRAY C90 a parallel inner product algorithm called PDOTB be used for the par-allelization of hybrid iterative methods containing sensitive values of inner products. Lastly it is shown that the number of iterations in which parallel hybrid iterative methods satisfy a certain convergence criterion depends on the number of processors to be used.

• 한국항행학회논문지
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• 제17권6호
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• pp.646-651
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• 2013

본 논문에서는 집중소자인 캐패시터 및 저항을 사용하여 특성조절이 가능한 소형 역-F 안테나를 제안하였다. 캐패시터는 안테나 크기 및 공진주파수의 조절을 위해 안테나 종단과 접지면 사이에 삽입된다. 반면에 $0{\Omega}$ 저항은 접지면과 단락되는 선로를 대체하여 구현한 것으로써, 단락점의 위치 이동은 안테나의 입력임피던스를 변화시킬 수 있다. 따라서 구조의 재설계 없이도 적절한 캐패시터 및 단락점 위치 선택으로 최적의 안테나 특성을 얻을 수 있다. 이와 같은 본 논문의 타당성 검증을 위하여 2.4 GHz 대역용 안테나를 설계 및 제작하여 지그비 무선 리모컨의 한 제품에 적용하였다. 적용된 안테나의 크기는 $8.5{\times}4.5mm^2$이며 재설계 없이도 대역폭 150 MHz 및 이득 2 dBi의 측정결과를 얻을 수 있다.

• 한국의류산업학회지
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• 제18권5호
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• pp.637-646
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• 2016

The goal of this study is to provide basic data on developing circular knit basic pattern for women in their 20's. Production conditions of circular knit product pattern making among domestic women's apparel industry was researched, and collected data on sizes and ease amounts from woven and circular knit pattern were compared and analyzed. According to the result of the survey, product measurements adjusted to the actual body size fit for the brand's image were used, and the common problem among manufacturers and consumers regarding circular knit products turned out to be change in size and form due to stretching. For the basic pattern of circular knit, stretching quality was reflected in the woven basic pattern based on plain stitch(single knit) and then dart was removed and ease amount was reduced. The result of looking into size and ease amount about woven and circular knit torso & sleeve block shows that there is a significant difference among chest circumference, hip circumference, bi-shoulder length, interscye back, interscye front, scye depth, upper arm circumference and wrist circumference, and it was clear that circumference and width on the areas around the wrist tended to fit around the body more when circular knit was used instead of woven fabric.

• International Journal of Oral Biology
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• 제44권3호
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• pp.89-95
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• 2019

Piperlongumine (PL) is a natural product found in long pepper (Piper longum). The pharmacological effects of PL are well known, and it has been used for pain, hepatoprotection, and asthma in Oriental medicine. No studies have examined the effects of PL on bone tissue or bone-related diseases, including osteoporosis. The current study investigated for the first time the inhibitory effects of PL on osteoclast differentiation, bone resorption, and osteoclastogenesis-related factors in RAW264.7 macrophages stimulated by the receptor activator for nuclear factor-${\kappa}B$ ligand (RANKL). Cytotoxicity was examined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and osteoclast differentiation and bone resorption were confirmed by tartrate-resistant acid phosphatase (TRAP) staining and pit formation analysis. Osteoclast differentiation factors were confirmed by western blotting. PL exhibited toxicity in RAW264.7 macrophages, inhibiting osteoclast formation and bone resorption, in addition to inhibiting the expression of osteoclastogenesis-related factors, such as tumor necrosis factor receptor-associated factor 6 (TRAF6), c-Fos, and NFATc1, in RANKL-stimulated RAW264.7 macrophages. These findings suggest that PL is suitable for the treatment of osteoporosis, and it serves as a potential therapeutic agent for various bone diseases.

• Natural Product Sciences
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• 제11권3호
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• pp.165-169
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• 2005

Chlorophytum borivilianum Baker (Antharicaceae) commonly referred as 'Safed Musli' has been widely used in the Indian traditional systems of medicine to treat various diseases like rheumatism apart from having immunomodulating property and is used as general tonic. It is also known as 'Ayurvedic viagra' for its aphordisiac properties. C. borivilianum was screened for the first time to determine its antioxidant activity, isolation of the sapogenins and standardization of the isolated sapogenin fraction using HPTLC. Potent antioxidant activity of ethanolic extract was found by their ability to scavenge DPPH (84.51%), hydroxyl radical (48.95 %), ferryl bi-pyridyl complex (84.53%) along with the inhibition of lipid peroxidation (67.17%) at $100\;{\mu}g/ml$ concentration. The ethanolic extract also exhibited significant inhibition of superoxide anion radical generated by photochemiluminescence. Thus, the potent antioxidant activity validates the innumerable therapeutic claims of the plant in the traditional system especially its use as a Rasayana drug.