• Environmental Analysis Health and Toxicology
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• 제22권2호통권57호
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• pp.129-135
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• 2007

Polycyclic aromatic hydrocarbons(PAHs) have been analyzed to assess vertical distribution of them with different land uses. The soils were collected from three layers; surface $(0{\sim}5cm)$, intermediate $(6{\sim}10cm)$, and deep $(11{\sim}15cm)$ layer, respectively considering land use; paddy, upland, and mountain in each site. Total 89 samples of soil from 10 sites were analyzed. Overall mean of ${\sum}PAHs$ were 137 (range $8.87{\sim}625{\mu}g\;kg^{-1}$), 203 (range $16.5{\sim}645{\mu}g\;kg^{-1}$), and $83.4{\mu}g\;kg^{-1}$ (range $6.65{\sim}667{\mu}g\;kg^{-1}$) for paddy, upland, and mountain soil, respectively. The dominant PAHs were fluoroanthene/benzo(b)fluoroanthene>pyrene>indeno(1, 2, 3-cd) pyrene in paddy, fluoroanthene/pyrene>benzo(b)fluoroanthene>chrysene in upland, and benzo(b)fluoroanthene>pyrene>chrysene in mountain soil, whereas the profile was quite similar for each other except that indeno(1, 2, 3-cd)pyrene and benzo(ghi)perylene are relatively higher in the paddy soils. Although the concentration gradient by depth was not observed in the paddy and upland soils because perturbation of soil layer by tillage, significant decrease was in the deep layer relative to the surface and intermediate layer. However, the concentration gradient of PAHs by soil depth was clearly shown in mountain soil without experiencing disturbance of tillage.

• 한국환경성돌연변이발암원학회지
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• 제24권3호
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• pp.128-136
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• 2004

CYP1A1 is known to be inducible by xenobiotic compouds such as polyciclic aromatic hydrocarbons(PAHs) and 2,3,7,8-tetrachloro-dibenzo-p-dioxin(TCDD). These chemicals have been identified worldwide and can have a significant impact on the human health and well being of human and wildlife. Given these issues, the detection and quantification of these chemicals in biological, environmental and food samples is important. First, we investigated the effect of on CYP1A1 promoter activity, 7-ethoxyresorufin-O-deethylase(EROD) activity and CYP1A1 mRNA expression induced by benzo(k)fluoranthene(B(k)F) in MCF-7 cells. We found that B(k)F significantly up-regulates the level of CYP1A1 prompter activity, EROD and CYP1A1 mRNA. When cells were treated with genistein, it was not changed that EROD and CYP1A1 mRNA, compared to that of control. However, genistein inhibited the B(k)F-induced CYP1A1 promoter activity and mRNA level at high concentration. Furthermore, in this study, effects of HDAC(histone deacetvlase) inhibitors on human prostate cancer cells proliferation were examined. HC-toxin, SAHA and TSA inhibited cell proliferation in PC3 cells. A novel HDAC inhibitor, IN2001 also suppressed the growth of PC3 cells. And IN2001 and SAHA increased S phase and G2/M phase at 12 hrs treatment but cells were arrested G0/G1 phase at 45 hrs treatment. The HC-toxin treatment for 24 hrs and 48 hrs increased G0/G1 at low concentration ($0.1\mu\textrm{m}$) but increased G2/M at more than concentration of $1\mu\textrm{m}$. TSA increased G2/M phase. These findings height the possbility of developing HDAC inhibitors as potential anticancer therapeutic agents for the treatment of prostate cancer.

• 한국토양비료학회지
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• 제40권3호
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• pp.196-200
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• 2007

영남농업연구소에 보관된 토양 시료의 분석결과 밀양지역 논토양에서의 PAHs 함량 1978년 이후 지속적으로 감소되고 있는 것으로 나타났다. 1978년에 $200{\mu}g\;kg^{-1}$을 넘어서 1980년에 최고농도($237{\mu}g\;kg^{-1}$)에 달하였으나, 이후 지속적으로 감소하여 2001년에는 $100{\mu}g\;kg^{-1}$ 수준까지 감소하였다. 이상의 결과는 PAHs의 발생원이 적은 지역의 농경지에서는 1980년대 이후 대체적으로 PAHs의 농도가 감소하고 있고, 오염물질(PAHs)의 대기이동에 의한 토양오염의 우려가 비교적 낮다는 것을 시사한다.