• Title/Summary/Keyword: Beginning of Life

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Fungal Diversity in Composting Process of Pig Manure and Mushroom Cultural Waste Based on Partial Sequence of Large Subunit rRNA

  • Cho, Kye-Man;Kwon, Eun-Ju;Kim, Sung-Kyum;Kambiranda, Devaiah M;Math, Reukaradhya K;Lee, Young-Han;Kim, Jung-Ho;Yun, Han-Dae;Kim, Hoon
    • Journal of Microbiology and Biotechnology
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    • v.19 no.8
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    • pp.743-748
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    • 2009
  • Fungal diversity during composting was investigated by culture-independent rDNA sequence analysis. Composting was carried out with pig manure and mushroom cultural waste using a field-scale composter (Hazaka system), and samples were collected at various stages. Based on partial sequence analysis of large subunit (LSU) ribosomal RNA (rRNA) and sequence identity values, a total of 12 different fungal species were found at six sampling sites; Geotrichum sp., Debaryomyces hansenii, Monographella nivalis, Acremonium strictum, Acremonium alternatum, Cladosporium sphaerospermum, Myriangium durosai, Pleurotus eryngii, Malassezia globosa, Malassezia restricta, Rhodotorula glutinis, and Fusarium sporotrichioides. Geotrichum sp. of the class Saccharomycetes was the most predominant fungal species throughout the composting process (185 out of a total of 236 identified clones, or 78.4%), followed by Acremonium strictum (7.6%), Monographella nivalis (5.1%), and Pleurotus eryngii (3.8%). The prevalence of Geotrichum sp. was the lowest (61.1%) at the beginning of composting, and then gradually increased to 92.5% after 10 days of composting.

Effects of Artemisia capillaris extract on disorders of hepatic functions and lipid metabolism in rats treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) (인진쑥 추출물이 다이옥신계 TCDD (2,3,7,8-Tetrachlorodibenzo-p-dioxin)에 노출된 흰쥐의 간 기능 및 지질대사에 미치는 효과)

  • Lee, Joon Ho;Zhang, Chun Lei;Bi, Shou Chao;Hwang, Seok Youn
    • Journal of Nutrition and Health
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    • v.46 no.3
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    • pp.207-217
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    • 2013
  • This study was conducted in order to investigate the effects of Artemisia capillaris (AC) extract on disorders of hepatic functions and lipid metabolism induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), an endocrine disrupter, using male rats (SD, five weeks old) for a period of three weeks. These 37 animals were divided into four groups. AC extract was added as 1.5% or 3% levels to basal diets, respectively. TCDD (40 ug/kg B.W) was administered by intraperitoneal injection into rats after a week from the beginning of the experiment. AC extract alleviated the increase of rat's relative liver weights induced by TCDD. Thymuses of all rats treated with TCDD were apparently shrunken by approximately 80%. Levels of white blood cells (WBC), red blood cells, hemoglobin, and hematocrits were significantly increased by treatment with TCDD, however, WBC tended to decrease by AC extract diets. In hepatic function, the elevation of glutamic oxalacetic transaminase activities by TCDD treatment was diminished by AC extract diets. Serum HDL-cholesterol levels were significantly elevated by AC extract diets. The apparent increase of triglyceride levels of rat livers induced by TCDD was significantly suppressed in the AC extract diet groups. Hepatic cytosolic catalase activities significantly decreased by treatment with TCDD showed a recovering trend by AC extract diets. In histochemical observation, the fat droplets and apoptosis of hepatocytes treated with TCDD were markedly alleviated by AC extract diets. These results indicated that AC could exert recovering effects on some disorders of hepatic functions, lipids metabolism, and antioxidant activities resulting from TCDD treatment.

The behaviour of strength and fatigue crack propagation of various steels in steel bridges (철강구조물 부재의 강도평가 및 피로균열진전거동)

  • Han, Seung-Ho;Kim, Jung-Kyu
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.21 no.10
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    • pp.1694-1701
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    • 1997
  • The residual safety assessment of steel structures, an important subject in practice, is given to much attention. Life prediction in the planning course of steel structures under fatigue loading is mainly based on fatigue design criteria resulting from S-N curves. But for any reason cracks have to be assumed due to fabrication failures or fatigue loading in service which can lead total fracture of structures. The life prediction can be carried out by means of fracture mechanics using Paris-Erdogan equation($da/dN=C {\cdot}{\Delta}K^m$). The paper presents results from charpy test to interpret transition behaviour of charpy energy($A_V$) in a wide temperature range and from constant-load-amplitude test to measure fatigue crack growth of various steels widely used in steel bridges since beginning of 20 centuries in Europe. In the normal service temperature range of steel bridges, the steel S355M shows higher maximum charpy energy($A_{Vmax}$) and lower transition temperature($T_{AVmax/2}$) than other steels considered. The C and m of Paris-Erdogan equation on the steels appear to be correlated, and to be affected by the R-ratios due to crack closure, especially at a low fatigue crack growth rate. Scanning electron microscopy analysis was carried out to interpret an influence of the crack closure effects on the correlation of C and m.

Diseases of the cultivated Porphyra at seocheon area (서천 해역 김 양식장의 갯병에 관한 연구)

  • Lee, Soon Jeong;Park, Sung Woo;Lee, Jong Hwa;Kim, Young Sik
    • Journal of fish pathology
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    • v.25 no.3
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    • pp.249-256
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    • 2012
  • Disease occurrence and dead cell ratio of Porphyra cultivated at Seocheon area were studied. Seaweed samples were collected monthly at 12 cultivating sites of Porphyra from Nov. 2008 to Mar. 2009. Although disease occurrence was low at the beginning of cultivation at the most of collecting sites, the occurrence increased with time. The most common symptom of Porphyra disease in this area was diatom attachment, followed by white rot disease. Licmophora sp. was the most common species of diatom and its attachment was the highest on November, the beginning of cultivation. Although the occurrence ratios of green spot disease and white rot disease were high on November, chytrid disease was increased at the end of cultivation period. Dead cell ratio of Porphyra thalli was different at each study site and cultivating time, but normally the ratio was high at the end of cultivating season. The changes of dead cell ratio could be related to the nutrition supplement and currents, and the more precise reason of dead cell ratio would be revealed by further research related to cultivating environment conditions.

Isolation of an Acetic Acid Bacterium Acetobacter pasteurianus CK-1 and Its Fermentation Characteristics (초산균 Acetobacter pasteurianus CK-1의 분리 및 발효 특성)

  • Bang, Kyu-Ho;Kim, Chae-Won;Kim, Chul-Ho
    • Journal of Life Science
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    • v.32 no.2
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    • pp.119-124
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    • 2022
  • To effectively isolate acetic acid bacteria for producing makgeolli vinegar, various products were researched, and Acetobacter pasteurianus CK-1, a strain that is excellent in acetic acid production, was finally isolated. The optimal growth temperature of the isolated strain was confirmed to be 30℃, and it grew well in the pH range of 5.5~6.5, with optimal growth at pH 6. A. pasteurianus CK-1 had the most active proliferation when the initial ethanol concentration in the medium was 4%, and growth was possible even at an ethanol concentration of 7%. When inoculating the isolated strain into makgeolli to induce acetic acid fermentation, the pH at the beginning of fermentation was 3.54, which was gradually lowered to 2.77 after 18 days of fermentation. The acidity was 0.75% at the beginning of fermentation and gradually started to increase from the 4th day of fermentation. The final acidity at the end of fermentation was 5.54%. In the vinegar fermented by inoculating A. pasteurianus CK-1, acetic acid content was detected to be as high as 3,575.7±48.6 mg%, and the malic acid and citric acid contents were 2,150.8±27.6 and 55.8±3.7 mg%, respectively. Further, it was confirmed that the content and ratio of the organic acids produced significantly differed depending on the type of inoculated bacterial strain. During acetic acid fermentation, the populations of yeast and A. pasteurianus CK-1 were inversely changed. In the initial stage of fermentation, yeast dominated, and after 10 days of fermentation, A. pasteurianus CK-1 slowly proliferated and reached stationary phase.

Effect of Vapor-dam Treatment on the Air Circulating oven Drying Characteristics of Bamboo Tubes (수증기댐 처리가 통죽(筒竹)의 송풍오븐건조 특성에 미치는 영향)

  • Lee, Nam-Ho;Jung, Hee-Suk;Hayashi, Kazuo;Li, Cheng-Yuan;Zhao, Xue-Feng;Hwang, Ui-Do
    • Journal of the Korean Wood Science and Technology
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    • v.35 no.1
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    • pp.11-16
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    • 2007
  • This study was carried out to investigate the effect of Vapor-dam treatment on drying drying rates, prevention of checks and V-shaped split during air circulating oven drying bamboo (Phyllostachys pubescens Mazel et Z) tube. It was shown that the drying time from green to around 7~8 % of moisture content was required less as drying schedule was more severe, and distinctly dominated by the drying rate during the initial drying stage. Area shrinkages in cross section and thickness shrinkages measured during air circulating oven drying test were very large. Surface checks and V-shaped splits were occurred in untreated samples just after the beginning of drying, while sixty seven percentages of all the Vapor-dam treated samples could be produced without drying defects. The V-shaped splits occurred in the Vapor-dam treated samples were influenced more by the sealing of the vapor evaporation through the cross section than drying schedule.

Ecological Study on the Seaweed Porphyra pseudolinearis Originated from the East Sea, Korea (동해안 고유종 긴잎돌김(Porphyra pseudolinearis)의 생태학적 연구)

  • Kim, Young-Dae;Lee, Ju;Son, Yong-Soo;Choi, Jae-Seok;Kim, Dong-Sam;Hong, Yong-Ki
    • Journal of Life Science
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    • v.14 no.2
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    • pp.331-338
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    • 2004
  • Growth and sexual differentiation of the seaweed Porphyra pseudolinearis Ueda have been investigated monthly in the intertidal zone of the East Sea, Korea. Young blades of P. pseudolinearis appeared at the beginning of October. Carp os pores were released at the end of November immediately after carposporangia formation. Then the thalli of P. pseudolinearis were extinguished at the end of March. Young thalli were budded through the stages of conchocelis and conchospore. Thalli showed lanceolate type in shape, cordate type in holdfast, absence of microscopic spinulate process and sexual generation. Ratios of length to width in female thalli ranged from 5.6 to 7.4 at the maturation in December and slightly decreased 3.3 to 4.8 in January and 4.9 to 7.3 in December while the ratios of male thalli ranged from 4.2 to 4.8 in January. On October 12, average five individuals were obsered in a quadrate (30 cm ${\times}$ 30 cm), 238$\pm$18 individuals for the maturation stage in December and then it was reduced to 150 individuals in February and 15 individuals in March. Average sex ratios for female, male and vegetative thalli were 31.3% 46.9% and 21.9% respectively in early December, the beginning time of sex maturation. The sex ratio of female and male thalli in December 17, changed to 69.4%, 30.6% respectively.

Life History of Locastra muscosalis (Walker) (Lepidoptera: Pyralidae) and Its Natural Enemies (벼슬집명나방의 생활사 및 천적 종류)

  • 박철하;이범영;이세표
    • Korean journal of applied entomology
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    • v.32 no.1
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    • pp.101-104
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    • 1993
  • The life history and host plants of Locastra muscosalis (Walker) which is a defoliating pest of walnut trees were studied mainly in Chungchongbuk-do province. Juglans nigra Linne, J. mandshurica Mixim., Carya illinoensis koch and Pterocarya stenoptera DC. were found to be new host plants. Locasta muscosalis (Walker) had one generation per year and the adults emerged form late June to late July with a peak emergence around July 10th. Females oviposited a mean of 560 eggs on the top surfaces of leaves. The duration of the egg stage was from 7 to 12 days. Young larvae fed on the leaves within webs that bound the leaves beginning in the middle of July. In the middle of September the fully matured larvae entered the soil -a cm below the soil surface, and the pupal period lasted 16 days on average. The natural enemies observed and/or reared from larvae of pupae were 3 species of parasitoids, 6 species of predators and an unidentified virus.

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A Biographical Study on Changeprocess of Values and Identities of the First-Generation Korean-German Females in Germany (재독한인1세대 여성의 가치관과 정체성의 변화과정에 대한 생애사 연구)

  • Yang, Yeung-Ja
    • Korean Journal of Social Welfare
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    • v.62 no.3
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    • pp.323-351
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    • 2010
  • Through the biographical approach, the current research purports to reconstruct the Changeprocess of values and identities on the lives of the first-generation Korean-German females in Germany from the transnational perspective. Ten interviews were conducted, using Schutze's autobiographicalnarrative interview. Interview data were analyzed through the application of Schutze's autobiographical-narrative interview and Mayring's qualitative content analysis. Findings showed that on the onset of emigration, their values centered around hybrid collectivism. Their life in the process of emigration was characteristic of a shift to hybrid individualism. Furthermore, the life at beginning of emigration was found to be characterized by a singular regional identity. The process of emigration was shown to mark the conversion into dual identity, dual regional and dual national. Some theoretical and practical suggestions for the emigrants' welfare were finally offered that were associated with the process of values and identities changes in their life.

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Seagrass (Zostera marina) Transplantation and Monitoring for Replacement Habitat (대체서식지 조성을 위한 거머리말 이식 및 모니터링)

  • Hong, Seong Jae;Choi, Chang Geun
    • Journal of Marine Life Science
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    • v.3 no.1
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    • pp.31-37
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    • 2018
  • We investigated natural habitat of seagrass and created replacement habitat to monitor for restoration of the habitat which is expected to be damaged at Cheonseong harbor in Busan. Depth of water for natural seagrass habitat at Cheonseong harbor was 1.2~3.1 m and the water temperature was 7.4℃, salt concentration was 29.1 psu and pH was 8.05 in January, 2013. The density of seagrass was 167.1±16.4 shoots m-2, the total length was 48.5±18.1 cm, the height of sheath was 9.1±2.8 cm and the width of leaf was 4.8±1.1 cm, respectively. We transplanted in December 2014 and monitored the habitat during 9 months after transplanted. In the beginning, the density of seagrass was decreased to 8.5 shoots patch-1 in January and was increased to 19.0 shoots patch-1 in April. The total height were 73.3±2.9~121.3±6.1 cm, the length of sheath were 9.6±0.6-21.0±1.2 cm, the width of leaf were 5.7±0.1~6.8±0.2 mm. It showed that all values were increased steadily until July and was decreased rapidly in August. Flowering shoot, which was not observed in the beginning of transplanting, started to be spotted in March and was continued to be seen during the monitoring period. We were able to observe seedling of germinated seagrass in seeds in the replacement habitat next year.