• Title/Summary/Keyword: Beef and pork

Search Result 406, Processing Time 0.044 seconds

Skeletal Muscle Troponin I (TnI) in Animal Fat Tissues to Be Used as Biomarker for the Identification of Fat Adulteration

  • Park, Bong-Sup;Oh, Young-Kyoung;Kim, Min-Jin;Shim, Won-Bo
    • Food Science of Animal Resources
    • /
    • v.34 no.6
    • /
    • pp.822-828
    • /
    • 2014
  • In this study, the existence of skeletal muscle troponin I (smTnI), well-known as a muscle protein in fat tissues, and the utilization of smTnI as a biomarker for the identification of fat adulteration were investigated. A commercial antibody (ab97427) specific to all of animals smTnI was used in this study. Fat and meat samples (cooked and non-cooked) of pork and beef, and chicken considered as representative meats were well minced and extracted by heating and non-heating methods, and the extracts from fat and meat tissues were probed by the antibody used in both enzyme-linked immunosorbent assay (ELISA) and immunoblot. The antibody exhibited a strong reaction to all meat and fat extracts in ELISA test. On the other hand, the results of immunoblot analsis revealed a 23 kDa high intensity band corresponding to the molecular weight of smTnI (23786 Da). These results demonstrate that the existence of smTnI in all animal fat tissues. Since there are monoclonal antibodies specific to each species smTnI, smTnI in fat tissues could be used as a biomarker to identify or determine animal species adulterated in meat products. Therefore, an analytical method to identify fraudulent fat adulteration can be developed with an antibody specific to each species smTnI.

Food Consumption Patterns of First Generation Korean-Americans in Hawaii

  • Han, ji-Sook
    • Preventive Nutrition and Food Science
    • /
    • v.3 no.1
    • /
    • pp.77-84
    • /
    • 1998
  • To evaluate food consumption patterns of first generation Korean-American in Hawaii, questionnaires were developed using sociodemographic questions and food frequency questionnaire, which included 139 food items most often consumed among Korean foods and American foods. The questionnaires surveyed 157 first generation Korean-Americans in Hawaii. Mean daily servings for the first generation Korean-Americans were calculated for 139 food items combined into 41 food groups based on similarity in nutrient composition and serving size. The food groups which were consumed in amounts over one serving per day for all subjects were rice, Kimchi , non-citrus fruit , vegetables, organge/green vegetables. oil. margarine and coffee/tea. All subjects consumed less than one serving of hotdogs, hamburgers, pizza and pancakes per week(0.14 serving per day). The most notable characteristic of food consumption for first generation Korean-Americans was that they consumed more Korean food such as rice, Kimchi, soybean paste(Deenjang), soybean curd and seaweed than American foods. Compared with other groups based on age and gender, younger men showed significantly(p<0.05) more frequent consumption of beef/pork, sausages /hams /bacons and hambergers. Older men were significantly(p<0.05) more likely to consume Doenjang and less likely to consume pizza and hamburgers. Daily servings were below the recommended level for thegrains /bread/cereals group and fats/oils/sweets group for all subjects . Fruits/vegetables group servings exceeded the recommeded 5 daily servings for younger men. In correlations of daily servings of selected foods among Korean foods and American food with sociodemographic characteristics, this study showed that the older the subjects and the shorter the stay in Hawaii, subjects were more likely to consume Kroean foods.

  • PDF

Estimation of Vitamin B6 Intake and Major Dietary Sources of Vitamin B6 in Elderly Koreans in the Seoul Area

  • Cho, Youn-Ok;Yun, Mie-Kyung
    • Preventive Nutrition and Food Science
    • /
    • v.9 no.1
    • /
    • pp.85-91
    • /
    • 2004
  • Dietary intakes and sources of vitamin B$_{6}$ in 72 healthy Korean elderly subjects (29 men,43 women, $\geq$57 years of age) living in the Seoul area were estimated using a modified Korean vitamin B$_{6}$ database. The dietary vitamin B$_{6}$ intake and food sources were estimated by the three day recall method with the help of a trained interviewer. Food portion sizes were estimated using standard household measures and published average portion sizes. The average daily vitamin B$_{6}$ intake was 2.06$\pm$0.46 mg/d in elderly men, and 1.94$\pm$ 0.47 mg/d in elderly women. Less than 5% of elderly men and 15% of elderly women consumed less than the Korean Recommended Dietary Allowance (RDA) of vitamin B$_{6}$. The average ratios of vitamin Be intake to daily protein intake were 0.03 $\pm$ 0.01 mg/g in both elderly men and women. The intake of vitamin B$_{6}$ was significantly (p <0.01 ∼ p<0.001) and positively correlated to intakes of all other nutrients in elderly women, but was not significantly correlated to intakes of several nutrients in elderly men. Foods from animal and plant sources provided 24% and 76%, respectively, of the total vitamin B$_{6}$. Major dietary sources of vitamin B$_{6}$ in Korean elderly were rice, soybean sprout, kimchi, pork, beef, potatoes, garlic, onion, anchovy and kochujang. The major sources accounted for most of the vitamin B$_{6}$ intake with the top 20 foods providing nearly 75∼76% of the total vitamin B$_{6}$ in Korean elderly./ in Korean elderly.

Survey on Menu Satisfaction and Preferences of High School Boarding Students in Gyeonggi Province (경기지역 일부 고등학교 기숙사생의 급식 메뉴 만족도와 기호도)

  • Shin, Sung-Min;Bae, Hyun-Joo
    • Korean journal of food and cookery science
    • /
    • v.26 no.4
    • /
    • pp.347-357
    • /
    • 2010
  • This study was assessed the menu satisfaction and preferences of high school boarding students in Gyeongggido. Data collection was carried out through surveys given to 300 high school boarding students; a total of 274 were usable. Statistical analyses were done using the SAS package program (version 8.2 for Windows). The results of this study are summarized as follows: 54.0% of respondents were freshman and 46.0% were sophomores: and 52.9% of respondents were male and 47.1% were female. According to the results of the menu satisfaction survey, males and females significantly differed on appropriate food temperature, freshness of food, harmonic combination of menu, menu variety, quality of ingredients, appearance of food, and frequency of supplied nutrition information. A significant difference regarding menu satisfaction was also found between grade level. First grade students were more satisfied than second grade students. Moreover, menu preferences were significantly high for omelet-rice, fried rice, pork-on-the-bone soup, deep-fried chicken, fruit salad, spicy chinese cabbage salad kimchi, chinese cabbage kimchi, and all desserts. On the other hand, pumpkin and rice porridge, beef and rice porridge, barley rice, soft tofu stew, soybean paste soup, braised japanese spanish mackerel, and sauteed squash were not significantly preferred. The results showed that high school boarding students' menu preferences differed depending on gender, grade, and boarding period. In conclusion, the results of this study should provide dieticians information for improving menu planning. In addition, foodservice satisfaction of the boarding school students can be improved by continuous menu improvement.

Antimicrobial Resistance Profile of Acinetobacter spp. Isolates from Retail Meat Samples under Campylobacter-Selective Conditions

  • Cha, Min-Hyeok;Kim, Sun Hee;Kim, Seokhwan;Lee, Woojung;Kwak, Hyo-Sun;Chi, Young-Min;Woo, Gun-Jo
    • Journal of Microbiology and Biotechnology
    • /
    • v.31 no.5
    • /
    • pp.733-739
    • /
    • 2021
  • Acinetobacter strains are widely present in the environment. Some antimicrobial-resistant strains of this genus have been implicated in infections acquired in hospitals. Genetic similarities have been reported between Acinetobacter strains in nosocomial infections and those isolated from foods. However, the antimicrobial resistance of Acinetobacter strains in foods, such as meat, remains unclear. This study initially aimed to isolate Campylobacter strains; instead, strains of the genus Acinetobacter were isolated from meat products, and their antimicrobial resistance was investigated. In total, 58 Acinetobacter strains were isolated from 381 meat samples. Of these, 32 strains (38.6%) were from beef, 22 (26.5%) from pork, and 4 (4.8%) from duck meat. Antimicrobial susceptibility tests revealed that 12 strains were resistant to more than one antimicrobial agent, whereas two strains were multidrug-resistant; both strains were resistant to colistin. Cephalosporin antimicrobials showed high minimal inhibitory concentration against Acinetobacter strains. Resfinder analysis showed that one colistin-resistant strain carried mcr-4.3; this plasmid type was not confirmed, even when analyzed with PlasmidFinder. Analysis of the contig harboring mcr-4.3 using BLAST confirmed that this contig was related to mcr-4.3 of Acinetobacter baumannii. The increase in antimicrobial resistance in food production environments increases the resistance rate of Acinetobacter strains present in meat, inhibits the isolation of Campylobacter strains, and acts as a medium for the transmission of antimicrobial resistance in the environment. Therefore, further investigations are warranted to prevent the spread of antimicrobial resistance in food products.

Using Status of Korean Agricultural Products in School Food Service and Dieticians' Perception (학교 급식의 우리 농축산물 이용 실태 및 이에 대한 영양사의 인식)

  • Yang, Il-Sun;Lee, Bo-Sook;Lee, So-Jung;Lee, Hae-Young;Jung, Hyun-Young
    • Journal of the Korean Society of Food Culture
    • /
    • v.21 no.2
    • /
    • pp.142-153
    • /
    • 2006
  • This study was planned to investigate the current using status of agricultural products in school food service both produced in Korea and imported from other countries and the dieticians' perception on using those. A total of 723 dieticians of 442 elementary schools and 381 middle high schools were surveyed in July, 2003 to perform the purposes of the study. A high using rate (93.4%) for Korean agricultural products was investigated. As for meat such as pork and chicken except beef and eggs, the using rate of Korean agricultural products was high. Almost 100% of vegetables and fruits, except tropical fruits such as banana, kiwi, orange and pineapple supplied for school food service were also Korean products. As for nuts and edible oil, the rate of using for Korean agricultural products was comparatively lower than those of other food items due to the price gap. Most dieticians rated the quality of the products produced in Korea higher than that of the imported products in the aspects of their freshness, taste, and safety. But they found no difference in the package condition of the products produced in Korea and of those imported from other countries. This result suggests that the package system should be improved for the agricultural products produced in Korea.

Development of Analytical Method and Monitoring of Veterinary Drug Residues in Korean Animal Products

  • Song, Jae-Sang;Park, Su-Jeong;Choi, Jung-Yun;Kim, Jin-Sook;Kang, Myung-Hee;Choi, Bo-Kyung;Hur, Sun Jin
    • Food Science of Animal Resources
    • /
    • v.36 no.3
    • /
    • pp.319-325
    • /
    • 2016
  • This study was conducted to determine the residual amount of veterinary drugs such as meloxicam, flunixin, and tulathromycin in animal products (beef, pork, horsemeat, and milk). Veterinary drugs have been widely used in the rearing of livestock to prevent and treat diseases. A total of 152 samples were purchased from markets located in major Korean cities (Seoul, Busan, Incheon, Daegu, Daejeon, Gwangju, Ulsan and Jeju), including Jeju. Veterinary drugs were analyzed by liquid chromatography-tandem mass spectrometry according to the Korean Food Standards Code. The resulting data, which are located within 70-120% of recovery range and less than 20% of relative standard deviations, are in compliance with the criteria of CODEX. A total of five veterinary drugs were detected in 152 samples, giving a detection rate of approximately 3.3%; and no food source violated the guideline values. Our result indicated that most of the veterinary drug residues in animal products were below the maximum residue limits specified in Korea.

The Importance of Food Open Challenge Test in Atopic Dermatitis: The Comparison of Allergy History, Skin-Prick Test, and Specific IgE Detection

  • Noh, Geun-Woong;Ji, Eun-Jung;Park, Jung-Nan;Kim, Kyung-Hee;Do, Min-Hee;Lee, Eung-Kyung;Lee, Sang-Sun
    • Nutritional Sciences
    • /
    • v.2 no.2
    • /
    • pp.119-124
    • /
    • 1999
  • Food allergies play an important role in Atopic Dermatitis (AD). Dietary manipulation is essential in the management of AD. However, there has been a paucity of data reporting the prevalence of food allergies in AD patients in Korea. In this study, the Food Open Challenge Tests (FOCT) were conducted to investigate food allergies in AD patients. The skin-prick test and the detection of specific IgE, as well as allergy history of patient were used to detect food allergies in all AD patients. Elimination diet was conducted for two weeks prior to FOCTs. The prevalence of food allergies by FOCT is as follows: milk (67.3%); chicken (64.2%); pork (62.8%); eggs (61.0%); beef(55.4%) ; wheat (52.0%) and soybean (45.2%). Allergenic food items in Korean AD patients were different from people in other foreign countries. The seven major foods those tested positively by FOCTs were completely eliminated from the replaced diets for two weeks, and were subsequently reintroduced one at a time. Results from FOCTs were not comparable with allergy history or skin-prick tests or specific IgE detection. The sensitivity and specificity of skin-prick tests and specific IgE detection were lower than FOCTs. Allergy history, skin-prick tests, and specific IgE detection are useful for the identification of food allergen but its clinical significance differed according to food items. Therefore, we conclude that even though a 10-day delay was necessary after food challenge, FOCT is a useful and valid method to confirm food allergies and may be essential for the effective control of food allergies for treatment of AD.

  • PDF

Survey Study on the Packed-lunch Boxes for Elementary School Children in Daejeon City (도시지역(都市地域)(대전(大田))일부 국민학교(國民學校) 아동(兒童)의 도시락 실태조사연구(實態調査硏究))

  • Rim, Young-Hie
    • Journal of Nutrition and Health
    • /
    • v.11 no.4
    • /
    • pp.39-48
    • /
    • 1978
  • The present study is concerned with nutritional status and palatability of the packed-lunch in elementary school children in Daejeon city. Rice and side dishes in the lunch box were weighed and the contained nutrients were analyzed by the Food Composition Table. The result of survey is as follows; 1) The means of nutritional values of 523 lunch boxes were calorie 562.48 Cal. percentage of the recommended dietary allowances.(79.11%), protein 19.16gr. (88.43%), animal protein 6.03gr. (83.50%), calcium 110.75mg (48.15%), iron 2.20mg. (56.15%), Vitamin A 328.16 I.U.. (49.22%), Vitamin $B_1$ 0.22mg. (59.45%), Vitamin $B_2$ 0.14mg (32.56%), Niacin 3.20mg. (68.08%), Vitamin C 9.79mg. (73.44%). All the nutrients are under the recommended dietary allowances. 2) The correlation between the boys and girls in the intake of protein, calcium, iron, Vitamin A, Vitamin $B_1$, Niacin index revealed statistical significancy at the level of P<0.01. 3) According to the intake of calorie, the ratio of carbohydrates, protein and fat is 78 : 14 : 8, which is excessive meal of carbohydrate. 4) Among the total intake of the protein consists of 30.20% for the boys and 32.90% for the girls. 5) The most favorite food for boys and girls from the packed-lunch is kimchi, egg, ham, sausage, cuttle-fish, laver and beef, and pork and pickles are most undesirable items. 6) The most favorite snack is bread, cake, ice cream, chocolate, fruits and milk. 7) Both boys and girls like Kimchi, fried and saute foods but dislike Namul (seasoned green vegetables) the most. 8. The side dishes of the packed·lunch lack in variety of cooking method.

  • PDF

Evaluation of Total Residues of Imidacloprid in Livestock Products from Domestic Markets by using a Simultaneous Analytical Method Based on QuEChERS (QuEChERS 기반 동시분석법을 이용한 국내 유통 축산물 중 Imidacloprid 총잔류량 평가)

  • Seung Won Lee;Ji Hyun Yoon;Ji Yu Kim;Da Jung Lim;Hyung Wook Jo;Joon Kwan Moon;Hye-Min Gwak;Hee-Ra Chang;In Seon Kim
    • Korean Journal of Environmental Agriculture
    • /
    • v.42 no.3
    • /
    • pp.220-230
    • /
    • 2023
  • Imidacloprid is a neonicotinoid insecticide widely used for insect control in a variety of crops. The evaluation of imidacloprid total residues in animal feeds derived from crop by-products is required to ensure the safety of livestock products. We performed simultaneous LC/MS/MS analyses of imidacloprid and its metabolites in five different livestock products including beef, pork, chicken, milk and egg from domestic markets. The methods for sample preparation and instrumental analysis were established by modifying QuEChERS method to meet the Codex guidelines. The methods generated 0.0035 mg/kg of the limit of determination (LOD), 0.01 mg/kg of the limit of quantitation (LOQ) and standard calibration linearity with >0.983 of the coefficients of determination (R2). The methods exhibited the recovery values of imidacloprid and its metabolites ranging from 65.66 to 119.27% without any interference between matrices. Imidacloprid total residues in the livestock products were found as values lower than the LOQ and maximum residue limits (MRLs). This study suggests that the methods are successfully applicable for the safety evaluation of imidacloprid total residues in livestock products from domestic markets.