• Journal of Life Science
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• v.21 no.3
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• pp.385-392
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• 2011

The otter (Lutra lutra) in Korea is classified as a first grade endangered species and is managed under state control. We performed a phylogenetic analysis of the otter that inhabits the Changnyeong, Jinju, and Geoje areas in Gyeongsangnamdo, Korea using mtDNA and microsatellite (MS) markers. As a result of the analysis using the 676-bp D-loop sequence of mtDNA, six haplotypes were estimated from five single nucleotide polymorphisms. The genetic distance between the Jinju and Geoje areas was greater than distances within the areas, and the distance between Jinju and Geoje was especially clear. From the phylogenetic tree estimated using the Bayesian Markov chain Monte Carlo analysis by the MrBays program, two subgroups, one containing samples from Jinju and the other containing samples from the Changnyeong and Geoje areas were clearly identified. The result of a parsimonious median-joining network analysis also showed two clear subgroups, supporting the result of the phylogenetic analysis. On the other hand, in the consensus tree estimated using the genetic distances estimated from the genotypes of 13 MS markers, there were clear two subgroups, one containing samples from the Jinju, Geoje and Changnyeong areas and the other containing samples from only the Jinju area. The samples were not identically classified into each subgroup defined by mtDNA and MS markers. It could be inferred that the differential classification of samples by the two different marker systems was because of the different characteristics of the marker systems used, that is, the mtDNA was for detecting maternal lineage and the MS markers were for estimating autosomal genetic distances. Nonetheless, the results from the two marker systems showed that there has been a progressive genetic fixation according to the habitats of the otters. Further analyses using not only newly developed MS markers that will possess more analytical power but also the whole mtDNA are needed. Expansion of the phylogenetic analysis using otter samples collected from the major habitats in Korea should be helpful in scientifically and efficiently maintaining and preserving them.

• Korean Journal of Environmental Biology
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• v.26 no.3
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• pp.159-169
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• 2008

This study was aimed to classify the intertidal macrozoobenthic community status after 2 years of Sea Prince oil spill, and oil spill effects along oil spreading track from heavily impacted to unaffected reference site. Field sampling was initiated in late February and continued through November 1998 seasonally, after 2.5 years of oil spill. 7 rocky sampling sites were selected among coastal regions coated and/or affected by the Sea Prince spilled oil. Identified species was 158 species, 65 family, 24 order, 9 class, 5 phylum. Mollusca was the dominant faunal group comprising 100 species (63.3%), and followed by 38 species of Crustacea (24.1%), 12 species of Echinodermata (7.6%), 5 species of Porifera (3.2%), and 3 species of Cnidaria (1.9%). On Dugpo of Sori Island, the fewest species was collected from 28 species to 35 species seasonally among sampling stations. But far away Dugpo toward Gamak Bay, the number of species increased, collecting the maximum on Sohwoenggan Island. At the wreck site of Sori Island, especially the species number of attached animals such as poriferans and anthozoans was very low compared to another site. The density and biomass on the higher tidal zone increased toward the low affected sites, but biomass on middle tidal zone decreased. The invertebrate biomass of study area was dependent on the sessile animals. The major dominant species were small-sized barnacles, Chthmallus challengeri, periwinkles, Littorina brevicula, mussels, Septifer virgatus, and so on. The biomass of C. challengeri and L. brevicula on the higher tidal zone was highest in the wreck site of Sori Island and decreased further and further. However, mussels on the middle tidal zone showed the inverse trends because of the larger individual size of mussel inhabited in Sori Island than those of another sites. As a result of community analysis, the effect of oil spill was not found distinctly. Several ecological indices and cluster analysis did not show the meaningful variation with oil track despite of the conspicuous differences among tidal heights. These indicate that the macrozoobenthic community level of oil spreaded zone recovered in some degree after the Sea Prince oil spill accident, but population or individual levels of dominant sessile animals took more recovery times.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.4 no.1
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• pp.71-79
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• 1999

As part of an on-going project investigating flux of materials in the Keum River Estuary, we have monitored seasonal variations of nutrients, suspended particulate matter (SPM), chlorophyll, and salinity since 1997. Meteorological data and freshwater discharge from the Keum River Dike were also used, Our goal was to answers for (1) what is the main factor for the seasonal fluctuation of nutrients in the Keum River Estuary? and (2) are there any differences in nutrient distributions before and after the Keum River Dike construction? Nitrate concentrations in the Keum River water were kept constant through the year. Whereas other nutrients varied with evident seasonality: high phosphate and ammonium concentrations during the dry season and enhanced silicate contents during the rainy season. SPM was found similar trend with silicate. During the rainy season, the freshwater discharged from the Keum River Dike seemed to dilute the phosphate and ammonium, but to elevate SPM concentration in the Keum Estuary. In addition, the corresponding variations of SPM contents in the estuarine water affected the seasonal fluctuations of nutrients in the Estuary. The most important source of the nutrients in the estuarine water is the fluvial water. Therefore, the distribution patterns of nutrients in the Estuary are conservative against salinity. Nitrate, nitrite and silicate are conservative through the year. The distribution of phosphate and ammonium on the other hand, display two distinct seasonal patterns: conservative behavior during the dry season and some additive processes during the rainy days. Mass destruction of freshwater phytoplankton in the riverine water is believed to be a major additive source of phosphate in the upper Estuary. Desorption processes of phosphate and ammonium from SPM and organic matter probably contribute extra source of addition. Benthic flux of phosphate and ammonium from the sediment into overlying estuarine water can not be excluded as another source. After the Keum River Dike construction, the concentrations of SPM decreased markedly and their role in controlling of nutrient concentrations in the Estuary has probably diminished. We found low salinity (5~15 psu) within 1 km away from the Dike during the dry season. Therefore we conclude that the only limited area of inner estuary function as a real estuary and the rest part rather be like a bay during the dry season. However, during the rainy season, the entire estuary as the mixing place of freshwater and seawater. Compared to the environmental conditions of the Estuary before the Dike construction, tidal current velocity and turbidity are decreased, but nutrient concentrations and chance of massive algal bloom such as red tide outbreak markedly increased.

• The Korean Journal of Malacology
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• v.23 no.2
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• pp.189-198
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• 2007

The reproductive cycle, egg capsules in the egg-mass, first sexual maturity, and sex ratio of the bladder moon, Glossaulax didyma ($R\ddot{o}ding$) were investigated. The gastropods collected from the intertidal zone of Biin Bay, Seocheon, Korea were studied by using histological analysis and morphometric data. The gonadosomatic index (GSI) of females and males began to increase in March and reached maximum in May. Then their values sharply decreased from late in May to August due to spawning. The condition index (CI) began to increase in February and reached maximum in May, then gradually declined in the spawning period. The CI calculated for determination of the spawning period was coincided with changes in the GSI and gonadal phases. Spawning occurred between late in May to August in females and early in May to August in males. Spawning peak was observed between July and August when the seawater temperature rose to 19 $^{\circ}C$. Reproductive cycle with the gonadal development phases of this species can be divided into five successive stages in females and four in males: in females, early active stage (December to February), late active stage (February to March), ripe stage (April recovery stage (August to November); in males, active stage (December to March), ripe stage (March to July), copulation stage (early May to August), and recovery stage (August to January). Fully matured oocytes were approximately 250-270 ${\mu}m$ in size. The egg-mass was a hat in shape, and a number of egg capsules were found in an egg-mass. An egg capsule was 0.53-0.57 mm in size. An embryo (veliger larva) hatched from an egg capsule. Percentage of first sexual maturity in females and males were over 50% for individuals that are 40.1-45.0 mm in shell radius, and 100% for those that are over 45.1 mm. The sex ratio of female to male was significantly different from 1:1 $(x^2\;=\;57.22,\;p\;<\;0.05)$.

• Korean Journal of Ichthyology
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• v.19 no.4
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• pp.306-317
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• 2007

Reproductive cycle with the gonad developmental phases, first sexual maturity, spawning frequency, sex ratio of the dusky mud hopper, Periophthalmus modestus were investigated by histological observations. Monthly variations of the gonadosomatic index (GSI) began to increase in May and reached a maximum in June when the gonad was getting mature during the period of higher ground (water) temperature-long day length. Changes in the GSI showed a negative correlation to the HSI, but coincided with the fatness index. The reproductive cycle can be classified into five successive stages: in female, early growing stage (April to May), late growing stage (April to May), mature stage (May to June), ripe and spent stage (June to August), and recovery and resting stage (August to March); in males, growing stage (April to May), mature stage (May to June), ripe and spent stage (June to August), and recovery and resting stage (August to March); According to the frequency distributions of egg diameters during the breeding season, Periophthalmus modestus is presumed to be a summer breeder, asynchronous group and polycyclic species to spawn 2 times or more during the spawning season. Total eggs and mature eggs in absolute fecundity and relative fecundity (per cm) increased with the increase of body length. Total eggs and mature eggs in absolute fecundity and relative fecundity (per g) did not increase with the increase of body weight. Percentages of first sexual maturity of females and males ranging from 5.1 to 5.5 cm in body length are over 50%, and 100% for fish over 7.1 cm in body length. The sex ratios of females to males over 5.1 cm in body length were not significantly different from a 1 : 1 sex ratio.

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.3
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• pp.159-170
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• 2006

This study was conducted to investigate the effects of two dry matter levels of liquid swine manure on dry matter yield and nutritive value of Italian ryegrass, rye and oats, and the chemical characteristics of soil in Jeju. This experiment tested in split plot design. Three forage crops (Italian ryegrass, rye and oats) were the main plot and four fertilizers (no fertilizer=T0, chemical fertilizer=T1, liquid swine manure with DM 2.7%=T2 and liquid swine manure with DM 5.9 %=T3) were the sub plots. Yield and nutrient contents of forage crops and soil properties were determined. Application of liquid swine manure containing 5.9% dry matter resulted in highest DM yield in all three forage crops species compared with the other treatments (p<0.01). Crude protein content(%) and crude protein yield(kg/ha) of forage crops were highest in rye compared with the other forage crops species(p<0.01). K and Mg contents of soil were higher(p<0.01) in rye than in the other species while Na contents was higher(p<0.01) in Italian ryegrass than others. Mg content of soil appeared higher in rye than in the others and higher(p<0.05) in forage crops applied with liquid manure containing 2.7% DM compared with the other species. $NO_3-N$ contents in soil was lower in rye than the other species and higher in species with chemical fertilizer. These findings indicate that most of liquid swine manure produced on local pig farms containing low levels of dry matter and other nutrients suggest a low efficiency of its use as a fertilizer. The liquid swine manure is recommended as a fertilizer for rye production in winter, compared with Italian ryegrass or oat.

• Journal of Aquaculture
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• v.9 no.3
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• pp.223-232
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• 1996

Growth and survival rate in shortnecked clam, Ruditapes philippinarum were studied from different seedling production areas, Hadong, Ulsan and Kochang to Kwangyang Bay, Korea, and three stocking densities (1,000, 1,500 and 3,000 individuals/$m^2$) of clam from Ulsan seedling production area were examined. During the experimental period, water temperature and specific gravity were ranged from 4.5 to $26.0^{\circ}C$ and from 1.0216 to 1.0248, respectively. Shell length was grown from $2.30{\pm}0.05\;to\;3,29{\pm}0.02$ cm in Hadong, $1.32{\pm}0.02\;to\;3.15{\pm}2.02$ cm in Ulsan, $1.24{\pm}0.03\;to\;3.18{\pm}0.02$ cm in Kochang. Kochang's seed was displayed fast growth rate than others. In different rearing densities, the lower density group was showed a rapid growth rate. Shell length from the 1,000 individuals per $m^2$ group grew longer than that from the 2,000 individuals group. Total weight from the 1,000 individuals group was higher than that from the 1,5()0 individual and the 2,000 individual groups. Survival rate by seedling production areas, Hadong, Ulsan and Kochang showed $69.4\%,\;63.8\%\;and\;41.7\%$, respectively. Survival rate by rearing density, 1,000, 1,500 and 2,000 individual groups showed $70.8\%,\;67.8\%\;and\;59.0\%$, respectively.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.10 no.4
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• pp.204-212
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• 2005

Recognition has emerged that nutrient inputs from the submarine discharge of fresh, brackish, and marine groundwaters into the coastal ocean are comparable to the inputs via river discharge. The coastal areas of the Korea peninsula and adjacent seas exhibit particular importance in the role of submarine groundwater discharge (SGD), in terms of the magnitude of SGD and associated continental material fluxes. For example, in the southern sea of Korea, SGD transports excess nutrients into the coastal regions and thus appears to influence ecosystem changes such as the outbreak of red tides. Around volcanic island, Jeju, which is composed of high permeability rocks, the amount of SGD is higher by orders of magnitude relative to the eastern coast of North America where extensive SGD studies have been conducted. In particular, nutrient discharge through SGD exerts a significant control on coastal ecosystem changes and results in benthic eutrophication in semi-enclosed Bang-du bay, Jeju. In the entire area of the Yellow Sea, tile submarine discharge of brackish groundwater and associated nutrients are found to rival the river discharges into the Yellow Sea, including those through Yangtze River, Han River, etc. In the eastern coast of the Korea peninsula, SGD is significantly higher during summer than winter due to high hydraulic gradients and due to wide distribution of high permeability sandy zones, faults, and fractures. On the other hand, in the estuarine water, downstream construction of the dam in the Nakdong River, SGD was highest when the river discharge was lowest (but water level of the dam was highest). This suggests that even though there is no visible freshwater discharge into this estuary, the discharge of chemical species is significant through SGD. On the basis of the results obtained from the coastal areas of the Korea peninsula, SGD is considered to be an important pathway of continental contaminants influencing tidal-flat ecosystems, red tides, and coral ecology. Thus, future costal management should pay great attention to the impact of SGD on coastal pollution and eutrophication.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.16 no.4
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• pp.305-315
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• 1983

A survey was carried out from July to December in 1979 and 1982 for the investigation of oceanographic conditions and phytoplankton in Seogwipo coastal area. Although a peculiar coastal sea water is formed by in flowing of fresh water, this is developed mainly in summer and disappeared or weakened in its strength after November. However, this coastal sea water covers only the surface while in mid and bottom layer open sea water is approached to the coast. Therefore, coastal and open sea water appears simultaneously in narrow sea area. Mean values of nutrient concentrates on surface layer during investigating period were $3.72{\sim}16.34{\mu}g-at/l$ in silicate, $1.98{\sim}5.53{\mu}g-at/l$ in nitrate and $0.34{\sim}0.90{\mu}g-at/l$ in phosphate. These showed slight differences among places but in general coastal side were lower than open sea side. Phosphates which is the lowest in concentrates among nutrients in Seogwipo coastal area shows almost similar value with Jinhae Bay but higher than open sea water around 10 mile south of Seogwipo. In general, seasonal changes of nutrients in investigating period shows a tendency of the lowest in October, increasing in November, and again slight decrease in December. As a phytoplankton fauna, 48 species, 1 variety and 2 breeds of Diatoms, 29 species, 3 varieties and 1 breed fo Dinoflagellates, and 1 species each of Chroococcus and Trichoceratium were found. Monthly predominant species are all neritic: Rhizosolenia sp. and Ceratium sp. in August, Chaetoceros sp., Ceratium sp. and Peridinium sp. in September, Astrionella sp. and Peridinium sp. in October, Astrionella sp., Navicula sp. and Chaetoceros sp. in November Among these, Rhizosolenia alata f. gracillima in August and Astrionella gracillima in November are remarkable predominant.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.1
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• pp.105-110
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• 2009

The effects of heat treated and herb added garlic (Allium sativum L.) on the antioxidant activities were investigated. Boiling ($100^{\circ}C$, 60 min), steaming ($100^{\circ}C$, 30 min), baking ($120^{\circ}C$, 10 min), or high temperature and high pressure (HTHP, $120^{\circ}C$, 20 min, 1.5 kgf/$cm^2$) were applied, and several herbs were added to garlic. Hunter color L-value of heated garlic was significantly decreased, compared to that of control (fresh garlic), whereas a-value and b-value were increased (p<0.05). In the texture profile analysis, hardness, chewiness and gumminess of heated garlic were decreased, whereas adhesiveness was increased. The antioxidant activities determined by DPPH and hydroxyl radical scavenging activities were decreased in HTHP garlic. The pungent taste and garlic odor were the weakest in HTHP garlic. Especially, the pungent taste of HTHP garlic was not detectable. Green tea among several herbs (bay leaf, cinnamon bark, pine needles) showed the highest DPPH radical scavenging activity. Addition of green tea to HTHP garlic was appropriate for decreasing antioxidative activity of HTHP garlic compared to fresh garlic. Based on these results, it was suggested that high temperature and high pressure treated garlic with green tea might be very useful as a substitute for odorless functional garlic products.