• KSBB Journal
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• v.22 no.3
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• pp.146-150
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• 2007

Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] microspheres were prepared using solvent evaporation technique. P(3HB-co-4HB) with 3.9 mol% 4HB was synthesized by fed-batch culture of Ralstonia eutropha. The effects of concentration and type of surfactant (Tween 80, sodium dodecylsulfate, and polyvinyl alcohol), addition of dispersion stabilizer (Acacia), concentration of polymer and model drug (bovine serum albumin) on particle size of the microspheres and their in vitro drug release characteristics were investigated. The average particle size of the microspheres decreased with the addition of dispersion stabilizer and increased with the concentration of surfactant, drug and polymer. Amount of drug release increased with decreasing particle size of the microspheres.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.5
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• pp.3000-3005
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• 2015

Reliable monitoring and diagnosis of industrial processes is quite important for in terms of quality and safety. The goal of fault diagnosis is to find process variables responsible for causing specific abnormalities of the process. This work presents a classification-based diagnostic scheme based on nonlinear representation of process data. The use of a nonlinear kernel technique is able to reduce the size of the data considered and provides efficient and reliable representation of the measurement data. As a filtering stage a preprocessing is performed to eliminate unwanted parts of the data with enhanced performance. The case study of an industrial batch process has shown that the performance of the scheme outperformed other methods. In addition, the use of a nonlinear representation technique and filtering improved the diagnosis performance in the case study.

• Nuclear Engineering and Technology
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• v.54 no.2
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• pp.456-461
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• 2022

Heavy metal pollution is caused due to anthropogenic activities and is considered as a serious environmental problem which endangers human health and environment. The present study deals with biosorption, an eco-friendly technique for the removal of heavy metal Zn(II) from aqueous medium. Various natural materials have been explored for the uptake of metal ions, where most of them are physically or chemically enhanced. Dry cowdung powder (DCP) has been utilized as a low-cost, environmentally friendly humiresin without any pre-treatment, thus demonstrating the concept of Green Chemistry. Batch biosorption studies using ⁶⁵Zn(II) tracer were performed and the impact of different experimental parameters was studied. Results revealed that at pH 6, 94 ± 2% of Zn(II) was effectively biosorbed in 5 min, at 303 K. The process was spontaneous and exothermic, following pseudo-second-order reaction. The mechanism of heavy metal biosorption employing green adsorbent was therefore elucidated in order to determine the optimal method for removing Zn(II) ions. DCP has a lot of potential in the wastewater treatment industry, as seen by its ability to meet 3A's affordability, adaptability, and acceptability criteria. As a result, DCP emerges as one of the most promising challengers for green chemistry and the zero-waste idea.

• Proceedings of the Botanical Society of Korea Conference
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• 1995.06a
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• pp.67-78
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• 1995

Monoclonal antibodies (MoAbs) are a highly diversified class of proteins with major research and commercial applications such as diagnostics and therapeutics. Currently, the dominant method for producing MoAbs is through the hybridoma technique. However, this technique is slow, tedious, labor intensive, and expensive. The production of MoAbs in cultured transgenic plant cells can offer some advantages over that in the over that in the mammalian systems. The media to cultivate plant cells are well defined and inexpensive. Contamination by bacteria or fungi is easily monitored in plant tissue cultures. Furthermore, these contaminants are usually not potent pathogens to human beings. In our interdisciplinary research efforts, heavy chain monoclonal antibody (HC MAb) was inserted into Ti plasmid vector and transferred into A. tumefaciens for the transformation in tobacco cells. It was found that 76% of the transformants produced HC MAb. The presence of HC MAb in the cell membrane fraction indicated that the signal peptide was functional and efficient. The change of the HC MAb concentration during a batch culture followed a similar trend as dry cell concentration, indicating that the production of HC MAb was growth related. The long-term repeated subcultures of 11 cell lines showed that there was no obvious trend of neither the decrease nor the increase of the productivity with the repeated subcultures.

• Journal of Korea Multimedia Society
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• v.15 no.11
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• pp.1319-1329
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• 2012

AGVs are more and more utilized nowadays and magnetic guided AGVs are most widely used because their system has low cost and high speed. But this type of AGVs requires high infrastructure building cost and has poor flexibility of navigation path layout changing. Thus it is hard to applying this type of AGVs to a small quantity batch production system or a cooperative production system with many AGVs. In this paper, we propose a vision based guideline interpretation technique that uses the cheap, easily installable and changeable color tapes (or paint) as a guideline. So a vision-based AGV with color tapes is effectively applicable to the production systems. For easy setting and changing of AGV navigation path, we suggest an automatic method for interpreting a complex guideline layout including multi-branches and joins of branches. We also suggest a trace direction decision method for stable navigation of AGVs. Through several real-time navigation tests with an industrial AGV installed with the suggested technique, we confirmed that the technique is practically and stably applicable to real industrial field.

• The KIPS Transactions:PartD
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• v.13D no.7 s.110
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• pp.1027-1038
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• 2006

There are many technical problems in the recommendation system based on very large database(VLDB). So, it is necessary to study the recommendation system' structure and the data-mining technique suitable for the large scale Internet shopping mail. Thus we design and implement the product recommendation system using k-means clustering algorithm and sequential pattern technique which can be used in large scale Internet shopping mall. This paper processes user information by batch processing, defines the various categories by hierarchical structure, and uses a sequential pattern mining technique for the search engine. For predictive modeling and experiment, we use the real data(user's interest and preference of given category) extracted from log file of the major Internet shopping mall in Korea during 30 days. And we define PRP(Predictive Recommend Precision), PRR(Predictive Recommend Recall), and PF1(Predictive Factor One-measure) for evaluation. In the result of experiments, the best recommendation time and the best learning time of our system are much as O(N) and the values of measures are very excellent.

• Environmental Engineering Research
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• v.25 no.1
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• pp.62-70
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• 2020

Here in this work, a 4-chlorophenol (4-CP)-degrading bacterial strain Bacillus subtilis (B. subtilis) MF447840.1 was isolated from the drain outside the Hyundai car service center, Agartala, Tripura, India. 16S rDNA technique used carried out for genomic recognition of the bacterial species. Isolated bacterial strain was phylogenetically related with B. subtilis. This strain was capable of breaking down both phenol and 4-CP at the concentration of 1,000 mg/L. Also, the isolated strain can able to metabolize five diverse aromatic molecules such as 2-chlorophenol, 2,4-dichlorophenol, 2,4,6-trichlorophenol, 4-nitrophenol, and pentachlorophenol for their growth. An extensive investigation was performed to portray the kinetics of cell growth along with 4-CP degradation in the batch study utilizing 4-CP as substrate. Various unstructured models were applied to evaluate the intrinsic kinetic factors. Levenspiel's model demonstrates a comparatively enhanced R² value (0.997) amongst every analyzed model. The data of specific growth rate (μ), saturation constant (K_S), and Y_X/S were 0.11 h^-1, 39.88 mg/L, along with 0.53 g/g, correspondingly. The isolated strain degrades 1,000 mg/L of 4-CP within 40 h. Therefore, B. subtilis MF447840.1 was considered a potential candidate for 4-CP degradation.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.44 no.7
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• pp.629-639
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• 2016

Batch process TRN(Terrain Relative Navigation) using an altimeter is a technique to correct position by correlating a series of periodically measured terrain height profile and terrain height candidate profile of the DEM(Digital Elevation Map). However, it is generally known that the performance of TRN is degraded when measured terrain height profile and terrain height candidate profiles of the DEM are similar at hill or repetitive terrain. In this paper, area based terrain slope roughness index[11] is applied and area based terrain curvature roughness index which can detect similarity of terrain in ROI(Region Of Interest) is proposed to overcome this problem. Applying terrain roughness indexes to terrain relative navigation system of lunar lander, it is shown that TRN using area based terrain roughness results in improved performance compared to conventional trajectory based method through simulation.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.15 no.1
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• pp.41-55
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• 2005

A group key agreement protocol allows a group of user to share a key which may later be used to achieve certain cryptographic goals. In this paper, we propose a new scalable two-round ID-based group key agreement protocol which would be well fit to a Pay-TV system, additionally. to the fields of internet stock quotes, audio and music deliveries, software updates and the like. Our protocol improves the three round poop key agreement protocol of Nam et al., resulting in upgrading the computational efficiency by using the batch verification technique in pairing-based cryptography. Also our protocol simplifies the key agreement procedures by utilizing ID-based system. We prove the security of our protocol under the Computational Diffie-Hellman assumption and the Bilinear Decisional Diffie-Hellman assumption. Also we analyze its efficiency.

• Journal of Microbiology and Biotechnology
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• v.6 no.3
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• pp.221-224
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• 1996

Pseudomonas putida BM01 was grown efficiently on glucose as the sole carbon source with a supply of a nitrogen source in pH-stat mode using a low setpoint limit. A final cell concentration of 100 g/l was obtained in 30 h of fed-batch cultivation by controlling glucose concentration within the range of 5-20 g/l and maintaining dissolved oxygen tension above 10$%$ saturation using pure oxygen. This high cell density culture technique is believed highly useful for the production of poly(3-hydroxyalkanoates) by this strain.