• KSCE Journal of Civil and Environmental Engineering Research
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• v.30 no.2C
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• pp.77-84
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• 2010

In planning underground roadway foundation on soft ground, deep cement mixing method (DCM) is employed. The proper mixing ratio using batch test and replacement rates that meet strength criteria are used for deep cement mixing column. Stiffness ratio and distance between deep cement mixing columns (C.T.C) are varied to find out influences on stress, displacement, and differential settlement. The replacement ratios that meet settlement criteria are 10~35%. As stiffness varies, stress reaches at 769.kPa that exceed criteria due to stress concentration when stiffness ratio difference is over 30. Also, when C.T.C is 5 m, stress spreads to soils, so C.T.C need to be considered carefully. The vertical displacement is 0.6~1.56 cm, and angular distortion is 1/909~1/510.

• Genomics & Informatics
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• v.21 no.3
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• pp.40.1-40.11
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• 2023

Microbial community profiling using 16S rRNA amplicon sequencing allows for taxonomic characterization of diverse microorganisms. While amplicon sequence variant (ASV) methods are increasingly favored for their fine-grained resolution of sequence variants, they often discard substantial portions of sequencing reads during quality control, particularly in datasets with large number samples. We present a streamlined pipeline that integrates FastP for read trimming, HmmUFOtu for operational taxonomic units (OTU) clustering, Vsearch for chimera checking, and Kraken2 for taxonomic assignment. To assess the pipeline's performance, we reprocessed two published stool datasets of normal Korean populations: one with 890 and the other with 1,462 independent samples. In the first dataset, HmmUFOtu retained 93.2% of over 104 million read pairs after quality trimming, discarding chimeric or unclassifiable reads, while DADA2, a commonly used ASV method, retained only 44.6% of the reads. Nonetheless, both methods yielded qualitatively similar β-diversity plots. For the second dataset, HmmUFOtu retained 89.2% of read pairs, while DADA2 retained a mere 18.4% of the reads. HmmUFOtu, being a closed-reference clustering method, facilitates merging separately processed datasets, with shared OTUs between the two datasets exhibiting a correlation coefficient of 0.92 in total abundance (log scale). While the first two dimensions of the β-diversity plot exhibited a cohesive mixture of the two datasets, the third dimension revealed the presence of a batch effect. Our comparative evaluation of ASV and OTU methods within this streamlined pipeline provides valuable insights into their performance when processing large-scale microbial 16S rRNA amplicon sequencing data. The strengths of HmmUFOtu and its potential for dataset merging are highlighted.

• The KIPS Transactions:PartB
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• v.14B no.5
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• pp.321-328
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• 2007

In this paper, we propose a fast and dynamic Region-of-Interest coding method using the adaptive code-block discrimination algorithm in JPEG2000 images which complements the implicit ROI coding method and the modified implicit ROI coding method. For reducing the time of discriminating the code block, the proposed method estimates the characteristics of the shape of ROI and makes the shape of boundaries, and classifies the patterns of each code block. The method improves the preferred processing and loss of wavelet coefficients of background within the ROI code blocks by adaptively classifying the code blocks with the percentage of content of the wavelet coefficients using the thresholds of ROI and background. Also, the priority control of wavelet coefficients of background within ROI code block supports the rapid ROI coding by processing in batch based on patterns unlike the existing methods that process with unit of wavelet coefficients. To show the usefulness of this method, we compared this to the existing methods. There is no difference in performance, but we confirmed very speedy in processing time.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.12
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• pp.23-32
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• 2020

This study investigated the thermal degradation and fading behavior of PTT dyed with vat dye and its dyeing ability. The PTT sample was dyed with vat dye using an acid treatment and pad-steam method to improve the dyeing performance. This method made dye particle nanosize and allowed it to penetrate the polymer material easily. The sample dyed using the pad-steam method showed level dyeing and enhanced dyeing affinity, compared to the batch-dyeing method. The degradation behavior of PTT dyed with vat dye after each heat and UV treatment was examined with the change in tensile strength or K/S value on the sample. The tensile strength and K/S values of the sample dyed with vat dye after the heat and UV treatment decreased with increasing temperature and exposure time. Although they showed high degradation under severe conditions, its rate constant was improved compared to the samples dyed with disperse dye. Consequently, acid treatment and the pad-steam method resulted in the introduction of vat dye on PTT. In addition, the PTT dyed with vat dye showed enhanced thermal and UV resistance because of their high molecular weight and chemical structure for stable adsorption behavior.

• Journal of KIISE:Software and Applications
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• v.33 no.2
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• pp.143-153
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• 2006

Microarray which enables us to obtain hundreds and thousands of expression of gene or genotype at once is an epoch-making technology in comparative analysis of genes. First of all, we have to measure the intensity of each gene in an microarray image from the experiment to gain the expression level of each gene. But it is difficult to analyze the microarray image in manual because it has a lot of genes. Meta-gridding method and various auto-gridding methods have been proposed for this, but thew still have some problems. For example, meta-gridding requires manual-work due to some variations in spite of experiment in same microarray, and auto-gridding nay not carried out fully or correctly when an image has a lot of noises or is lowly expressed. In this article, we propose Hierarchical Grid Alignment algorithm for new methodology combining meta-gridding method with auto-gridding method. In our methodology, we necd a meta-grid as an input, and then align it with the microarray image automatically. Experimental results show that the proposed method serves more robust and reliable gridding result than the previous methods. It is also possible for user to do more reliable batch analysis by using our algorithm.

• Journal of Life Science
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• v.16 no.3 s.76
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• pp.534-539
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• 2006

In the ozone disinfection unit process of a piston type batch reactor with continuous ozone analysis using a flow injection analysis (FIA) system, the CT values for 1 log inactivation of Cryptosporidium parvum by viability assays of DAPI/PI and excystation were $1.8{\sim}2.2\;mg/L{\cdot}min$ at $25^{\circ}C$ and $9.1mg/L{\cdot}min$ at $5^{\circ}C$, respectively. At the low temperature, ozone requirement rises $4{\sim}5$ times higher in order to achieve the same level of disinfection at room temperature. In a 40 L scale pilot plant with continuous flow and constant 5 minutes retention time, disinfection effects were evaluated using excystation, DAPI/PI, and cell infection method at the same time. About 0.2 log inactivation of Cryptosporidium by DAPI/PI and excystation assay, and 1.2 log inactivation by cell infectivity assay were estimated, respectively, at the CT value of about $8mg/L{\cdot}min$. The difference between DAPI/PI and excystation assay was not significant in evaluating CT values of Cryptosporidium by ozone in both experiment of the piston and the pilot reactors. However, there was significant difference between viability assay based on the intact cell wall structure and function and infectivity assay based on the developing oocysts to sporozoites and merozoites in the pilot study. The stage of development should be more sensitive to ozone oxidation than cell wall intactness of oocysts. The difference of CT values estimated by viability assay between two studies may partly come from underestimation of the residual ozone concentration due to the manual monitoring in the pilot study, or the difference of the reactor scale (50 mL vs 40 L) and types (batch vs continuous). Adequate If value to disinfect 1 and 2 log scale of Cryptosporidium in UV irradiation process was 25 $mWs/cm^2$ and 50 $mWs/cm^2$, respectively, at $25^{\circ}C$ by DAPI/PI. At $5^{\circ}C$, 40 $mWs/cm^2$ was required for disinfecting 1 log Cryptosporidium, and 80 $mWs/cm^2$ for disinfecting 2 log Cryptosporidium. It was thought that about 60% increase of If value requirement to compensate for the $20^{\circ}C$ decrease in temperature was due to the low voltage low output lamp letting weaker UV rays occur at lower temperatures.

• Journal of Environmental Health Sciences
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• v.40 no.2
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• pp.114-126
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• 2014

Objectives: We aimed to develop a measurement method of five metabolites of trichloroethylene (TCE) in a concurrent biological sample, e.g., trichloroacetic acid (TCA), dichloroacetic acid (DCA), S-(1,2-dichlorovinyl) glutathione (DCVG), S-(1,2-dichlorovinyl)-L-cysteine (DCVC), and N-Acetyl-S-(1,2-dichlorovinyl)-L-cysteine (NAcDCVC) and to validate the method before application to pharmacokinetic study. Methods: TCE metabolites were simultaneously analyzed using high performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS/MS) with as little as 50 ${\mu}L$ of serum and urine. DCA, TCA and NAcDCVC were extracted with diethyl ether, while DCVC and DCVG were extracted by solid phase extraction. This method was validated according to the guidelines for bioanalytical method validation of the Korean National Institute of Toxicological Research. Then, we determined the five metabolites in five strains of mice at 24 hr after exposure to 1 g TCE /kg body weight. Results: The limits of detection for the five metabolites in biological samples ranged from 0.001 to 0.076 nmol/mL, which is comparable to or better than those previously reported. Most calibration curves showed good linearity ($R^2=0.99$), and between-batch variation was less than 20% expressing acceptable robustness and reproducibility. Using this method, we found TCA and DCA were detected in all test mice at 24 hr after the oral administration while NAcDCVC and DCVC were detected in some strains, which showed strain-dependent metabolism of TCE. Conclusions: The present method could provide robust and accurate measurements of major key metabolites of TCE in biological media, which allowed concurrent analysis of TCE metabolism for limited amounts of biospecimens.

• Journal of the Korea Society for Simulation
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• v.23 no.4
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• pp.51-64
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• 2014

Small and medium Enterprises (SMEs), which have a manufacturing method of small quantity batch production produce goods using a general-purpose equipment and attached auxiliary tools. Many previous studies have focused on finding the effective resource allocations for improving the firms' productivity. It is very important for SMEs to keep costs low in assigning jobs to each resource, because they should meet the future uncertain demand of consumers under the limited budget. Using the concept of salvage cost, this paper proposes how to effectively allocate the tasks to main resources in the production process. The salvage cost is defined that purchasing cost minus decrease in value by workload, the method considering this is expected to reduce total purchasing costs during business period. To validate the effect of the proposed method, we proceed the real case study targeting on S company, PCB manufacturer to compare purchase amounts and its costs between the allocation proposed based salvage cost and current allocation method of current S company. As a results, In short-term (3 year) business period, salvage allocation have remarkable superior outcome to existing method, but gradually have cancelled out the effects in long-term (8 year) plans. Unlike the cycle allocation method, there exists the idle-equipments in allocation based salvage value. we additionally analyze the profits with respect to rental strategy of them during business period.

• Korean Journal of Construction Engineering and Management
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• v.22 no.4
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• pp.20-28
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• 2021

The Domestic Construction Industry is in the process of changing from a one-sided production method centered on the contractor to a production method in which the order and the contractor can collaborate. Depending on the delivery method, the bid/success method, the contract method, and the degree of business involvement of the order may also vary. In this thesis, in order to introduce 'Construction Management at Risk' suitable for the domestic environment, domestic and foreign institutional analysis, bid process analysis, contract fulfillment and management analysis, post management analysis, and related laws and regulations for legalization In order to suggest a plan for the efficient operation of the system, a system and case analysis of domestic and international construction responsibility type construction project management was conducted. 'Construction Responsible Construction Project Management' defined in the Framework Act on the Construction Industry is divided into the main contract transfer contract, which is a service contract performing pre-con work, and the main contract, which is construction project management and construction contract. Therefore 'Construction Management at Risk' should be regarded as one of the ordering methods rather than a successful bid system, as in the case of overseas, and legalization as a bidding system equivalent to design/construction batch bid and technology proposal is necessary for introduction. In order to introduce 'Construction Management at Risk' suitable for the domestic environment, domestic and foreign institutional analysis, bidding process analysis, contract fulfillment and management analysis, and follow-up management analysis are conducted. A method for efficient operation was suggested so that it can be applied in the domestic market while maintaining the original merits of this system.

• Journal of Korean Society of Environmental Engineers
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• v.36 no.1
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• pp.7-12
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• 2014

The adsorption characteristics of Pb(II) and Cu(II) by the manganese oxide-coated activated carbon (MOAC) were investigated by series of batch experiments. MOAC was prepared by three types of manufacturing methods such as chemical precipitation method (CP), hydrothermal method (HT) and supercritical method (SC). Pseudo-second-order and Langmuir models adequately described kinetics and isotherm of Pb(II) and Cu(II) adsorption on the experimented adsorbents. These results indicated that heavy metal ions were chemically adsorbed onto uniform monolayered adsorption sites. The coating of manganese oxide enhanced the adsorption capacities of AC. And adsorption capacities of Pb(II) and Cu(II) were significantly affected by the manufacturing method of MOAC. The highest adsorption performance was obtained by using SC, followed by HT and CP, which is caused from high uniformity and amount of manganese oxide coated onto AC induced by high temperature and pressure. These results show that MOAC can be used as an effective adsorbent to remediate heavy metal contaminated environment.