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Effect of Agitation on Production of Methylan and Rheological Characteristics of Methylan Fermentation Broth (다당류, 메틸란, 발효밴잉액의 점성특성과 메틸란 생산에 미치는 교반속도의 영향)

  • Oh, Deok-Kun;Lim, Hyun-Soo;Kim, Jung-Hoe
    • Applied Biological Chemistry
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    • v.38 no.3
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    • pp.191-195
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    • 1995
  • Production of a high viscosity exoploysaccharide, methylan, by Methylobacterium organophilum from methanol was carried out in fed-batch cultures and the rheological properties of methylan fermentation broth were studied. Bacterial biomass showed little influence on viscosity, but the accumulation of methylan caused the increase of viscosity. With proceeding fermention, the viscosity at the same concentration of methylan was significantly increased and methylan solution showed slightly higher pseudoplasticity. The composition changes of methylan were investigated at various fermentation times. Contents of total sugar, reducing sugar and methylan were decreased but contents of acids(pyruvic acid, uronic acid and acetic acid) were increased with the culture time. It was considered that the increased content of acids resulted in the increase of the hyrodynamic domain in the solution due to charge repulsion. Consequently, the solution viscosity increased in propotion to the acids contents of methylan. Cell growth and methylan production were severely decreased by the limitation of dissolved oxygen. However, the cellular activity for methylan production was almost constant regardless of the level of dissolved oxygen. As a result, the high speed of agitation increased the methylan production, the specific production rate of methylan, and the methylan yield of the cell.

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A Scale-Up Test for Preparation of AlN by Carbon Reduction and Subsequent Nitridation Method (탄소환원질화법에 의한 AlN 제조 규모확대 시험결과)

  • Park, Hyung-Kyu;Kim, Sung-Don;Nam, Chul-Woo;Kim, Dae-Woong;Kang, Moon-Soo;Shin, Gwang-Hee
    • Resources Recycling
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    • v.25 no.5
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    • pp.75-83
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    • 2016
  • AlN powder was prepared by carbon reduction and subsequent nitridation method through the scale-up experiments of 0.7 ~ 1.5 kg per batch. AlN powder was synthesized using the mixture of $Al_2O_3$ powder and carbon black at $1,550{\sim}1,750^{\circ}C$ for 0.5 ~ 4 hours under nitrogen atmosphere (flow rate of nitrogen gas: $10{\sim}40{\ell}/min$) at $2.0{\times}10^{-1}Torr$. Experimental results showed that $1,700{\sim}1,750^{\circ}C$ for the reaction temperature, 3 hr for reaction time, and $40{\ell}/min$ for the flow rate of nitrogen gas were the optimal conditions. Also, in order to remove carbon in the synthesized AlN, the remained carbon was removed at $650{\sim}750^{\circ}C$ for 1 ~ 2 hr using horizontal tube furnace. The results showed that 1 : 3.2 mol ratio of $Al_2O_3$ to carbon black, reaction temperature of $750^{\circ}C$, reaction time of 2 hours, rotating speed of 1.5 rpm under atmosphere condition were the optimal conditions. Under these conditions, high-purity AlN powder over 99% could be prepared: carbon and oxygen contents of the AlN powder were 835 ppm and 0.77%, respectively.

A $CST^+$ Tree Index Structure for Range Search (범위 검색을 위한 $CST^+$ 트리 인덱스 구조)

  • Lee, Jae-Won;Kang, Dae-Hee;Lee, Sang-Goo
    • Journal of KIISE:Databases
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    • v.35 no.1
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    • pp.17-28
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    • 2008
  • Recently, main memory access is a performance bottleneck for many computer applications. Cache memory is introduced in order to reduce memory access latency. However, it is possible for cache memory to reduce memory access latency, when desired data are located on cache. EST tree is proposed to solve this problem by improving T tree. However, when doing a range search, EST tree has to search unnecessary nodes. Therefore, this paper proposes $CST^+$ tree which has the merit of CST tree and is possible to do a range search by linking data nodes with linked lists. By experiments, we show that $CST^+$ is $4{\sim}10$ times as fast as CST and $CSB^+$. In addition, rebuilding an index Is an essential step for the database recovery from system failure. In this paper, we propose a fast tree index rebuilding algorithm called MaxPL. MaxPL has no node-split overhead and employs a parallelism for reading the data records and inserting the keys into the index. We show that MaxPL is $2{\sim}11$ times as fast as sequential insert and batch insert.

A Study on the Quality Properties When Applying Recycled Aggregate Concrete for the Construction Standard Mitigation (건축기준 완화를 위한 순환골재 콘크리트 적용 시의 품질 특성 연구)

  • Woo, Gyeong-Sub;Kim, Jung-Ho;Lee, Sea-Hyun;Oh, Jung-Keun
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.24 no.3
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    • pp.63-69
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    • 2020
  • The volume of construction waste material from the entire waste material volume in Korea is approximately 47.3% to take the biggest ratio, and from them, the waste concrete takes up approximately 62.8% that recycling is an urgent issue to address. Therefore, the government recommends more diverse and broader facilitation of the recycled aggregate in order to promote recycling the construction waste materials. In addition, when using concrete recycled aggregate in building, building standard such as floor area ratio and building height are being mitigation. The standard is a condition that mitigation the floor area ratio by up to 15% when using up to 25% of concrete recycled aggregate. Therefore, this study reviewed the relaxation of construction of construction standards when using concrete recycled aggregate in order to actively recommend the use of concrete recycled aggregate. And using the recycled coarse aggregate among the recycled aggregate, the appropriate mixing time in the batch plant according to the substitution rate was derived. In addition, using recycled aggregate admixture in order to improve the drying shrinkage, did comparative analysis about physical and mechanical property of concrete.

Extraction of ${\beta}$-carotene from Ascidian Tunic [Halocynthia roretzi] using Supercritical Carbon Dioxide and Co-solvent (초임계 이산화탄소를 이용만 우렁쉥이 껍질로부터 ${\beta}$-carotene 추출)

  • Kang, In-Sook;Youn, Hyun-Seok;Park, Ji-Yeon;Chun, Byung-Soo
    • KSBB Journal
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    • v.21 no.3
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    • pp.194-198
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    • 2006
  • Dried raw Ascidians(Halocynthia roretzi) shells harvested from fish farms in southern coast area in Korea were used to extract ${\beta}$-carotene using supercritical carbon dioxide($SCO_2$) and with ethanol as a co-solvent at the range of temperatures and pressures, from 25 to $65^{\circ}C$ and 100 to 350 bar respectively. The size of the dried Ascidians shells was around $850{\mu}m$. The system used this study was a semi-batch flow type high pressure unit. The efficiency of ${\beta}$-carotene extraction using $SCO_2$ with and without co-solvent, ethanol, influenced to pressure and temperature changes. The highest solubility of ${\beta}$-carotene in $SCO_2$ was 1.35 mg/g for ${\beta}$-carotene at $35^{\circ}C$ and 350 bar. With addition of 2(v/v%) ethanol the recovery of ${\beta}$-carotene was 93%. As a result of using n-hexane and methanol for rinse, at $35^{\circ}C$ and 350 bar the amount of ${\beta}$-carotene by methanol rinse was 5 times higher than that of n-hexane rinse.

Investigation of the fate of inorganic nitrogens in the near field of bank filtration site (강둑여과지 주변의 밭에 살포된 무기질소의 거동 연구)

  • 공인철;배진희;안호준;권오억;김승현;이철희;박영규
    • Journal of Korea Soil Environment Society
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    • v.3 no.1
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    • pp.11-20
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    • 1998
  • Inorganic nitrogens resulting from fertilizers are possible contaminants of bank-filtered ground water, which is an alternative source of safe drinking water. We conducted a laboratory study to determine the fate of inorganic nitrogens in the near field of bank filtration sites and we consequently illustrated the effects of these possible contaminants on the water quailty of bank filtration. Soil properties were found to be well equivalent to those of other cultivated field soils in Korea. Surface soil pH was about 4.3 which is slightly lower than average level. Overall, diverse concentrations of $NO_3$-N and $NH_3$-N were measured, and $NO_2$-N was nearly undetected. $NH_3$-N level in the field decreased, while $NO_3$-N increased along with increasing depth of unsaturated zone. Numbers of viable and nitrifying bacteria ranged from 6.73$\times$$10^{6}$to 10.7$\times$$10^{6}$ and 0.44$\times$$10^{4}$ to 5.21$\times$$10^{4}$ respectively, and both of them were highly correlated ($R^{2}$=0.992). Nitrifying potential assayed by batch test showed relatively lower than other reported results. The oxygen uptake potential of surface and subsurface soil was measured in the presence of ${(NH_4)_2}$$SO_4$. The results demonstrated that surface soil required shorter lag period and uptaked more oxygen than subsurface's.

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A Study on the Treatment of Wastewater from Ion Removal Process for Purifying Electrocoat Paint in the Bath by Use of Reverse Osmosis (역삼투압을 이용한 전착도료 정제공정폐수처리에 관한 연구)

  • 김진성
    • Membrane Journal
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    • v.8 no.2
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    • pp.77-85
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    • 1998
  • To treat effectively EDIR (electrodeposition ion removal) wastewater in terms of CO$_{Mn}$ 1,500~2,000 ppm generated from aluminum painting process, a RO (reverse osmosis) process was designed and installed to recover and reuse the concentrated solvent sent back to the electrocodeposition tank while the permeate reused as rinse water. A RO system in which three polyamide-spiral wound modules ($102\Phi \times 1,016L$ mm) connnected in series had been running to treat 20 m$^3$ in waste volume in 3 days batch operation at the condition of system recovery of 30 %, applied pressure 11.5 $kg_f/cm^2$ and room temperature. During 42 hours continuous operation leading to 5-fold decrease in waste volume, nearly constant permeation flux of 390 l/m$^2$-hr was maintained and the permeate with average CO$_{Mn}$, 300 ppm was obtained which could be used for washing the remaining paint solution in ion-exchange tower instead of demineralized water. Also COD$_{Mn}$ rejection as a function of running time was observed to be in the range of 78~87 % and the observed solvent rejections for ethyl cellusolve, buthyl cellusolve and n-butanol were 79 %, 87 % and 70 %, respectively.

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Biosynthesis and Degradation of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in Alcaligenes sp. SH-69 (Alcaligenes sp. SH-69에서의 Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) 생합성 및 분해)

  • Ryu, Kang-Eun;Choi, Gang Guk;Park, Sang Kyu;Kim, YoungBaek;Rhee, Young Ha
    • Korean Journal of Microbiology
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    • v.34 no.4
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    • pp.219-224
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    • 1998
  • The cyclic metabolism of poly(3-hydroxyhutyrate-co-3-hydroxyvalerate) synthesized from glucose by Alcaligenes sp. SH-69 in the presence or absence of new carbon substrate was investigated. In batch culture, the content and weight average molecular weight of the copolymer already stored in the cell decreased rapidly when there was no other carbon source available. After the depletion of carbon source, the amount of high molecular weight copolymer decreased more rapidly than that of low molecular weight copolymer, and as a result, average molecular weight distribution shifted to the lower value. The addition of a mixture of glucose and levulinic acid when the initial carbon substrate, glucose, was nearly depleted supported the continual increase in cell mass and the accumulation of poly(3HB-co-3HV) with high molar fraction of 3HV. However, solvent fractionation of the polymer with acetone revealed the degradation of pre-existing polyhydroxyalkanoale (PHA) in parallel with the synthesis of PHA from new carbon substrate. Even though PHAs obtained from each substrate alone were the copolymer of 3HB and 3HV, it was found that the polymer accumulated in the cells grown by sequential feeding was mainly physical mixture of two poly(3HB-co-3HV) copolymers containing different molar fractions of 3HV.

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Influence of Ammonium Phosphate on Mycelial Morphology during Submerged Cultivation of Ganoderma lucidum (영지의 액체배양에 있어서 균사체 형태에 미치는 Ammonium Phosphate의 영향)

  • Lee, Kyu-Min;Lee, Shin-Young
    • The Korean Journal of Mycology
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    • v.29 no.2
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    • pp.91-98
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    • 2001
  • The mycelial morphology during submerged cultivation of Ganoderma ludium using by air-lift fermenter system were analyzed by image processing system and the characterization of mycelial morphology were investigated. In submerged culture using medium with different ammonium phosphate concentrations, the various morphological forms of G. lucidum mycelium were observed. The filamentous forms such as non-branched long filamentous mycelium, non-branched short mycelium, branched long filamentous mycelium, branched short mycelium, entangled mycelium and clump were observed, and also, and also, the pelleted forms such as smooth pellet, rough pellet and hollow rough pellet were observed. The mycelial morphology was changed from the filamentous to the pelleted forms by addition of ammonium phosphate. The fractal dimensions of pelleted and filamentous forms were 1.05 and 1.3, respectively, while the fractal dimension of mixtures of pelleted and filamentous forms was 1.16. Therefore, the fractal dimension was found to be more effective index for the detection of the mycelial morphology and morphological change during batch cultivation. The circularity was also found to be useful for evaluating the surface growth of pelleted mycelium.

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Esterification of Lactic Acid with Alcohols (젓산과 알코올간의 에스테르화 반응)

  • Kim, Jong-Hwa;Han, Jee-Yeun;Lee, Sang-Wha
    • Applied Chemistry for Engineering
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    • v.16 no.2
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    • pp.243-249
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    • 2005
  • Esterification of lactic acid with alcohols catalyzed by Amberlyst-type ion exchange resins and sulfuric acid was carried out in a batch reactor with total /or partial recycle of distilled condensates, respectively. The esterification of lactic acid in the total-recycling reactor (n-butanol/lactic acid = 4, $100^{\circ}C$) was promoted by decreasing the residual water and increasing the mole ratio of n-butanol/lactic acid. Also, it was confirmed that methanol with simple structure and tert-butanol with superior substitution reactivity were more effective in increasing the conversion of esterification reaction, compared to ethanol, n-butanol, and iso-butanol. In a partial-recycling reactor (n-butanol/ammonium lactate = 4, $115^{\circ}C$), the conversion of ammonium lactate into butyl lactate with 1.0 wt% Amberyst-type resins was higher in comparison to that with 0.2 mol $H_2SO_4$ (per 1.0 mol ammonium lactate). The esterification was gradually occurred during the initial stage of reaction in the presence of solid catalyst, whereas the initial addition of $H_2SO_4$ did not affect the initial rate of esterification reaction because of ammonium sulfate formation by the neutralizing reaction of ammonium lactate with sulfuric acid.