• The Korean Journal of Mycology
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• v.33 no.1
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• pp.54-57
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• 2005

Lee and author (2000) rearanged the list of mushrooms recorded in Korea, containing 1,554 species 388 genera from 90 families for the systematic classification of Korean mushrooms at Korea Forest Research Institute. In September, 2002, Lee, Tai-Soo and Kab-Hee Yoon made public ‘The Index of Korea-Japan Mushroom names in Korea’ that listed 1,610 species from 396 genera, 90 families and 23 orders of mushrooms recorded in Korea. Among the listed taxa, 1,369 species, 297 genera, 65 families, 15 orders belong to Basidiomycotina and 241 species, 99 genera, 25 families and 8 orders belong to Ascomycotina. A total of 56 species were newly added to the list of recorded Korean mushrooms for a period of 2 years. On the other hand, a total of 75 species, 3 varieties and 2 formae were added to the list of recorded Korean mushrooms by the author, and they were published by many researchers from 2001 to 2004. Among the published taxa, one forma, 20 species, 16 genera, 12 families belong to Ascomycotina and one forma, 3 varieties, 55 species, 33 genera, 17 families belong to Basidiomycotina.

• The Korean Journal of Mycology
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• v.35 no.1
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• pp.11-27
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• 2007

This study was conducted to investigate the diversity of higher fungi in NaeJangSan National Park from April 2004 to November 2006. The obtained results from investigation were as follows. The total of 5 classes, 19 orders, 60 families, 168 genera and 418 species (including 10 families, 13 genera and 15 species unrecorded) including saprophytic and ectomycorrhizal fungi was investigated. The higher fungi were classified into 47 families, 143 genera and 384 species in Basidiomycotina, 9 families, 19 genera and 28 species in Ascomycotina and 4 families, 6 genera and 6 species in Myxomycota. It was turned out that most of the higher fungi belong to Hymenomycetidae in Basidiomycotina, for which 34 families 122 genera, and 353 species were observed. Dorminant species belonged to Tricholomataceae(64 species) Russulaceae(39 species), Polyporaceae(36 species) and Boletaceae(36 species). The mushroom occurrence of higher fungi was closely related to climatic conditions such as high air temperature and lots of rainfall from July to September. The environment which has a favorable influence of mushroom occurrence was air temperature, relative humidity and rainfall of climatic environment.

• The Korean Journal of Mycology
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• v.20 no.1
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• pp.51-57
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• 1992

Mitochondria in Pleurotus ostreatus were isolated and purified by stepped sucrose density gradient centrifugation, to compare the effects of organic compound on the activities of mitochondrial ATPase in Basidiomycotina with those in mammalian cell. The effects of N, N'-dicycio-hexylcarbodiimide (DCCD), carbonyl cyanide m-chlorophenylhydrazone (CCCP), sodium azide and aurovertin known as compounds to be related to electron transfer system in mitochondria were studied. A activity of mitochondrial ATPase was inhibited by 64%, 57% and 53% in the presence of 0.25 mM DCCD, 0.02 mM sodium azide and 1.5 $({\mu}g/mg\;of\;protein)$ aurovertin B, respectively. It was stimulated by 22% in the presence of 0.15 ${\mu}M$ CCCP.

• Journal of Microbiology
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• v.34 no.1
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• pp.38-39
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• 1996

The internal regions of nuclear small subunit rRNA from 6 plaeurotus species and 5 Pleurotus ostreatus strains were amplified by PCR and sequenced. The DNA sequences of 8 Pleurotus strains (P. ostreatus NFFA2, NFFA4501, NFFA4001, KFFA4001, KFCC11635, P florida, P. florida, P. sajor-cuju, P. pulmonarius, and P. spodoleucus) were idential, but P. cornucopiae differed from them in two bases out of 605 bases. However, p[hylogenetic analysis of the sequences by DNA-distance matrix and UPGMA methods showed that P. ostreatus NFFA2m1 and NFFA2m2, known as mutants of P. ostreatus NFFA2, belonged to anther group of Basidiomycotina, which is close to the genus Auricularia. The difference of the SSU rDNA sequences of P. cornucopiae from other Pleurotus species tested corresponds to the difference of mitochondrial plasmid type present in Pleurotus species as observed by Kim et al. (1993, Korean J. Microbiol. 31, 141-147).

• The Korean Journal of Mycology
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• v.23 no.4 s.75
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• pp.305-309
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• 1995

To investigate optimal conditions for the protoplast formation and regeneration of Phanerochaete chrysosporium, preparations of three enzymes were used to liberate protoplasts from its 20 hrs-old mycelium on cellophan membrane covered agar media. Novozym 234 alone with 0.6M sucrose was the most effective for isolation of protoplasts from the mycelium with 3hrs incubation time at $39^{\circ}C$ in shaking condition of 120 rpm. The poly-R medium stabilized with 0.6M mannitol was the best for regeneration of the protoplasts.

• Journal of Microbiology
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• v.33 no.2
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• pp.136-141
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• 1995

From a cluster of structural rRNA genes which has previsouly been cloned (Hwang and Kim, in submission; J. Microbiol. Biotechnol.), a 1.0-kb Eco RI fragment of DNA which shows significant homology to the 25S and rRNA s of Tricholoma matsutake was used for sequence analysis. Nucleotide sequence was bidirectionally determined using delection series of the DNA fragment. Comparing the resultant 1016-base sequence with sequences in the database, both the 3'end of 25S-rRNA gene and 5S rRNA gene were searched. The 5S rRNA gene is 118-bp in length and is located 158-bp downstream of 3'end of the 25S rRNA gene. IGSI and IGS2 (partial) sequences are also contained in the fragment. Multiple alignment of the 5S rRNA sequences was carried out with 5S rRNA sequences from some members of the subdivision Basidiomycotina obtained from the database. Polygenetic analysis with distance matrix established by Kimura's 2-parameter method and phylogenetic tree by UPGMA method proposed that T. matsutake is closely related to efibulobasidium allbescens. Secondary structure of 5S rRNA was also hypothesized to show similar topology with its generally accepted eukaryotic counterpart.

• The Korean Journal of Mycology
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• v.25 no.1 s.80
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• pp.26-29
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• 1997

Pyrimidine auxotrophic basidiospores of Pleurotus sajor-caju were selected using positive selection method. Wild type basidiospores could not grow on minimal medium containing the pyrimidine analog 5'-fluoro-orotic acid (5'-FOA) whereas pyrimidine auxotrophs grew normally. After treatment of basidiospores with ultraviolet light, a total of 13 pyrimidine auxotrophic basidiospores were isolated among 24 5'-FOA resistant mutants. These mutants require the pyrimidine such as uracil, cytosine, thymine. Mating type group and growth rate of their mutants were determined.

• The Korean Journal of Mycology
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• v.19 no.3
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• pp.167-174
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• 1991

More than 300 specimens of higher fungi were collected from both Mt. Daeduck and Mt. Daesung areas in Kangwon Province during the period from 13 to 19 August, 1990. These fungi were identified and classified into 4 orders, 15 families, 35 genera, 52 species and 1 variety. Dominant species were considered to be Collybia confluens in Tricholomataceae, and Russula emetica in Russulaceae of Agaricales, and Phlogiotis hevelloides in Auriculariales throughout our survey areas. Phlogiotis hevelloides (Fr.) Martin. (장미주걱목이) and Pseudohydnum gelatinosum (Scop. ex Fr.) P. Karst. (혓바늘목이) were newly described in Korea.

• The Korean Journal of Mycology
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• v.19 no.1
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• pp.27-31
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• 1991

Formation of conidium, an asexual organ on the hyphae, was examined from ten species of Pleurotus. Conidiospores of them were distinguished into two types of spores; an elliptical and a globose spores. Dikaryotic hyphae of ten species and monokaryotic hyphae of three species were observed to produce conidiospore. Conidia were observed on the hyphae grown on mushroom complete agar medium but were not on mushroom minimal agar medium. Two aconidial mutants were obtained by the ultraviolet irradiation.

• The Korean Journal of Mycology
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• v.48 no.3
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• pp.251-271
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• 2020

Diversity and community composition of bulb-associated fungi of Fritillaria Cirrhosae Bulbus source plants, which are used in traditional chinese medicine, in the eastern Himalaya-Hengduan Mountains, southwestern China, were estimated based on the internal transcribed spacer rDNA sequence analysis, using host plant species, geographic area, and plant phenology as variables. A total of 1,486 fungal sequences assigned to 251 operational taxonomical units (OTUs) were obtained from the bulbs. Fungal OTUs comprised 96.41% Ascomycotina, 3.52% Basidiomycotina, and 0.07% Zygomycotina. Sordariomycetes, Hypocreales, and Nectriaceae were the most frequent fungal lineages at each taxonomic rank. Fusarium, Ilyonectria, Tetracladium, Leptodontidium, and Tomentella were the top OTU-rich genera. Fusarium sp. 03, Ilyonectria rufa, Fusarium sp. 08, Ilyonectria sp. 03, and Leptodontidium orchidicola 03 represented the most frequent OTUs. Fusarium spp. were the most frequent general taxa. The distribution of fungal community exhibited preferences for host plant species, geographic area, and plant phenology. These findings are the foundation of our research on culturing and active metabolites of bulb-associated fungi of Fritillaria Cirrhosae Bulbus source plants.