• Journal of Korean Neurosurgical Society
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• 제30권sup2호
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• pp.189-196
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• 2001

목 적 : 교모세포종은 흔한 원발성 뇌종양이며 생물학적 특성상 빠른 성장률을 보이는 것 외에 침습성이 강하여 종양과 인접한 부분을 파괴 시킬 뿐 아니라 직접접촉하지 않는 부분의 파괴도 일어나게 되어 치료 예후가 매우 불량한 것으로 되어 있다. 광역학치료는 광감각제를 이용하며 광감각제는 적절한 파장의 광원에 노출되면 세포 내에서 산소독성 물질을 생성하여 세포괴사를 유도하는 것이 주 살해작용의 기전이다. 본 실험에서는 사람의 신경교종 세포주인 U87 세포를 이용하여 실험관 내에서 광감각제 photofrin을 이용한 광역학치료가 종양의 침습성에 미치는 영향을 알아보고 동시에 이를 뇌종양치료의 새로운 방법으로 사용될 수 있는지의 여부를 알아보고자 하였다. 재료 및 방법 : 교모세포종 세포주인 U87 세포를 여러 농도의 photofrin으로 처리한 후 632nm $100mJ/cm^2$의 고정된 광선조건에서 본 실험을 시행 하였으며 Microculture tetrazolium(MTT) assay를 이용하여 세포 살해능력을 측정하고 침습성은 matrigel artificial basement membrane assay 및 tumor spheroid fetal rat brain aggregate(FRBA) confrontation assay를 이용하여 측정하였다. 결 과 : MTT assay를 이용하여 측정한 광역학 치료의 세포살해능력은 $100mJ/cm^2$의 광선 세기에서 광감각제인 photofrin의 농도에 비례하여 세포 살해 능력을 보였다. Matrigel artificial basement membrane assay 를 이용한 종양 침습성 검사에서 광역학치료가 종양침습의 억제효과를 나타냄을 알 수 있었으며 특히 세포 살해능력을 별로 보이지 않았던 photofrin 농도 2.5ug/ml에서 뚜렷한 침습억제효과를 나타내고 있었다(p<0.05). tumor spheroid fetal rat brain aggregate(FRBA) confrontation assay에서는 brain aggregate의 파괴와 종양의 침습을 관찰할 수 있었는데 파괴의 모양은 생체내에서 보이는 것과 비슷 하였다. 또한 그 정도와 범위는 처리된 Photofrin의 농도에 비례하여 종양침습이 억제됨을 알 수 있었다. 결 론 : 이러한 결과를 종합해 보았을 때 PDT는 종양세포의 침습성에 중요한 역할을 함을 알 수 있었으며 PDT 는 세포 살해능력뿐 아니라 침습성에도 영향을 미침으로써 종양 치료에 유용한 방법으로 사용될 수 있을 것으로 사료된다.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2005년도 춘계 국제심포지엄 및 학술대회
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• pp.51-82
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• 2005

To compare the pulmonary toxicity between ultrafine colloidal silica particles (UFCSs) and fine colloidal silica particles (FCSs), mice were intratracheally instilled with 3 mg of 14-nm UFCSs and 230-nm FCSs and pathologically examined from 30 mill to 24 hr post-exposure. Histopathologically, lungs exposed to both sizes of particles showed bronchiolar degeneration and necrosis, neutrophilic inflammation in alveoli with alveolar type II cell proliferation and particle-laden alveolar macrophage accumulation. UFCSs, however, induced extensive alveolar hemorrhage compared to FCSs from 30 min onwards. UFCSs also caused more severe bronchiolar epithelial cell necrosis and neutrophil influx in alveoli than FCSs at 12 and 24 hr post-exposure. Laminin positive immunolabellings in basement membranes of bronchioles and alveoli of UFCSs treated animals was weaker than those of FCSs treated animals in all observation times. Electron microscopy demonstrated UFCSs and FCSs on bronchiolar and alveolar wall surface as well as in the cytoplasm of alveolar epithelial cells, alveolar macrophages and neutrophils. Type I alveolar epithelial cell erosion with basement membrane damage in UFCSs treated animals was more severe than those in FCSs treated animals. At 12 and 24 hr post-exposure, bronchiolar epithelia cells in UFCSs treated animals showed more intense vacuolation and necrosis compared to FCSs treated animals. These findings suggest that UFCSs has greater ability to induce lung inflammation and tissue damages than FCSs.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제31권5호
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• pp.440-453
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• 2005

Objectives : To develop a bioactive membrane for guided bone regeneration (GBR), the biocompatibility and bone regenerating capacity of the cellulose membrane obtained from the Ascidians squirt skin were evaluated. Materials and methods : After processing the pure cellulose membrane from the squirt skin, the morphological study, amino acid analysis and the immunoreactivity of the cellulose membrane were tested. Total eighteen male Spraque-Dawley rats (12 weeks, weighing 250 to 300g) were divided into two control (n=8) and another two experimental groups (n=10). In the first experimental group (n=5), the cellulose membrane was applicated to the 8.0 mm sized calvarial bone defect and the same sized defect was left without cellulose membrane in the first control group (n=4). In the another experimental group (n=5), the cellulose membrane was applicated to the same sized calvarial bone defect after femoral bone graft and the same sized defect with bone graft was left without cellulose membrane in the another control group (n=4). Each group was sacrificed after 6 weeks, the histological study with H&E and Masson trichrome stain was done, and immunohistochemical stainings of angiogenin and VEGF were also carried out. Results : The squirt skin cellulose showed the bio-inductive effect on the bone and mesenchymal tissues in the periosteum of rat calvarial bone. This phenomenon was found only in the inner surface of the cellulose membrane after 6 weeks contrast to the outer surface. Bone defect covered with the bioactive cellulose membrane showed significantly greater bone formation compared with control groups. Mesenchymal cells beneath the inner surface of the bioactive cellulose membrane were positive to the angiogenin and VEGF antibodies. Conclusion : We suppose that there still remains extremely little amount of peptide fragment derived from the basement membrane matrix proteins of squirt skin, which is a kind of anchoring protein composed of glycocalyx. This composition could prevent the adverse immunological hypersensitivity and also induce bioactive properties of cellulose membrane. These properties induced the effective angiogenesis with rapid osteogenesis beneath the inner surface of cellulose membrane, and so the possibilities of clinical application in dental field as a GBR material will be able to be suggested.

• 한국수산과학회지
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• 제33권5호
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• pp.403-407
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• 2000

징거미새우의 정소는 간췌장의 배면 측상부에서 심장사이에 위치하며, 한 쌍의 소엽으로 이루어져 있고 각 소엽의 선단은 서로 연결되어 있다. 그리고 각 소엽은 수많은 세정관이 결합조직으로 연결되어 있다. 각 세정관은 생식세포와 지주세포가 밀착되어 있는 생식세포대를 제외한 부분은 낮은 입방상피에 의해 내강이 형성되어 있고, 세정관의 제일 바깥은 단층편평상피로 둘러싸여 있다. 세정관 사이에는 결합조직 외에 Leydig cell-like cell도 관찰된다. 단층편평상피세포는 얇은 측면으로 연결되어 있고 종종 이웃한 세포와 포개져 있다. 지주세포는 생식세포에 비해 그 수가 현저히 적고, 대부분 생식세포대의 가장자리에 위치하고 있다. 지주세포의 세포질은 기저판과 접해 있어 기저면과 생식세포 사이는 지주세포의 원형질에 의해 분리되어 있다. 지주세포의 핵은 대부분 각져 있고, 크고 현저한 인이 핵의 중심부에 위치하고 있다. 낮은 입방상피세포는 세정관의 기저판과 접하고 있는 기저면과 일부는 생식세포대와 내강 사이에 위치하고 있다. 입방상피세포의 세포질에서는 횡 방향의 크리스테가 발달된 미토콘드리아, 층상구조의 조면소포체 그리고 Golgi 복합체들이 매우 발달되어 있다. 그리고 조면소포체와 Golgi 복합체의 볼록한 형성면 사이에는 전이소낭이, Golgi 복합체의 성숙면에서는 분비소낭이 관찰되며, 상피세포의 선단에 수많은 홈들이 존재하는 점으로 보아 exocytosis에 의해 내강으로의 물질 분비가 이루어지는 것으로 판단된다.

• Animal cells and systems
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• 제3권2호
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• pp.173-180
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• 1999

A blue biliprotein, insecticyanin (INS), has been purified from the last instar larval hemolymph of Agrius convolvuli by ultracentrifugation, Sephadex G-100 gel permeation chromatography, and preparative electrophoresis. The molecular mass of INS was estimated to be 26 kDa and the N-terminal sequence of INS revealed high similarity to that of Manduca sexta. Results of Western blotting and autoradiography indicated that INS is synthesized by the epidermis and released into the hemolymph. In contrast to the INS reported in other insects, Agrius convolvuli INS contained a small amount of lipid, predominately consisting of triacylglycerol. Subcellular localization of INS was determined using protein-A gold particles linked to secondary antibodies (anti-rabbit Ig). INS was heavily accumulated in the cytoplasmic inclusion body (CIB). CIBs showed a variety of shapes from rod to globule and generally surrounded the nucleus. They were mostly located near the basement membrane and especially abundant in the intersegmental membrane.

• 대한세포병리학회지
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• 제17권1호
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• pp.32-37
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• 2006

The objective of this study was to evaluate the cytomorphologic features of histologically confirmed clear cell carcinoma of the ovary and to evaluate the applicability of scrape or fine-needle aspiration cytology in making an intraoperative diagnosis. We reviewed scrape or fine-needle aspiration cytology findings in tissues taken from 6 patients with clear cell carcinoma of the ovary. The cytologic diagnosis was based primarily on findings in alcohol-fixed, hematoxylin-eosin (H-E) stained smears. The formation of material resembling a basement membrane was a characteristic finding in these smears. This extracellular hyaline material was stained light pink with H-E and was frequently found within tumor cell clusters as well as in the background material. Multinucleated giant cells were found occasionally. Each tumor cell had an abundant, clear, or granular cytoplasm with a distinct cellular membrane. Scrape cytology is a simple and rapid supportive method and could be helpful in diagnosing clear cell carcinoma of the ovary, especially when marked artifacts appear in the frozen section.

• Journal of Chest Surgery
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• 제22권3호
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• pp.402-415
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• 1989

Extracorporeal circulation leads to functional disorder and structural damage of organs, especially hematologic and pulmonary system, mainly by sequestration of neutrophils and deposition of macrophages at lung. Then, proteases are secreted, which insult vascular basement membrane of pulmonary capillary and alveolar septa of the lung. Among these, the most important protease at lung is elastase, because major component of lung is elastin. For prevention of lung injury, inactivators or antidotes to elastase should be necessary and Alpha 1-Proteinase Inhibitor is the elastase inactivator. Clinical experimental study was carried out to investigate the immediate postoperative change of serum Alpha 1-PI level following cardiopulmonary bypass for 20 heart cases [congenital 16 cases, acquired 4 cases] and 10 control [subtotal gastrectomy] cases. Also preliminary study was performed for 31 cases of open heart patients. The results were as follows: l. Immediate postoperative serum levels of Alpha 1-PI was significantly decreased at open heart surgery group [P< 0.005], but not decreased at control group. 2. There were no significant difference in change of serum Alpha 1-PI level between and membrane and bubble oxygenator group.Z 3. There were no significant difference in changes of serum Alpha 1-PI level between CHD and AHD. Alpha 1-PI is consumed at lung during cardiopulmonary bypass and increase after operation compensatedly and protect multiple organic damage especially lung. Therefore, Alpha 1-PI can be indicator for evaluation of prevention and treatment of pump-lung syndrome.

• Preventive Nutrition and Food Science
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• 제1권2호
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• pp.168-173
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• 1996

The effect of methanol soluble fraction(MSF) of kimchi on the proliferating and differentiating activity of normal rat mammary epithelial cells or organoids in culture were studied. Reconstituted basement membrane, Matrigel, supported the growth and development several different multicellular structures from mammary organoids. The five type colonies of multicellular structures, stellate, ductal, webbed, squamous, and lobulo-ductal colonies, were observed in Matrigel culture. In methanol extract groups, webbed colonies were more and squamous colonies were less than control group. and the lobulo-ductal colonies which is known that it formed in well differentiated mammary epoithelial cells were developed more in MSF treated group than control group. These results showed that methanol extract of kimchi affected on the proliferation and differentiation of normal rat mammary epithelial cells cultured in serum free medium condition.

• Animal cells and systems
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• 제8권3호
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• pp.197-203
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• 2004

CD30 is a member of tumor necrosis factor receptor (TNFR) superfamily and has pleiotropic functions including cell activation, proliferation, differentiation, and death, depending on cell types and stage of differentiation. Although CD30 expression has been described mainly in hematopoietic tissues, several types of nonhematopoietic tumors including embryonic carcinoma and germ-cell tumors express CD30. We examined CD30 distribution in the testis and epididymis from wild type and CD30-deficient mice. In the testis, spermatogonia, spermatocytes and Sertoli cells expressed CD30, but not in spermatids. Spermatogonia and spermatocytes near the basement membrane strongly reacted to anti-CD30. In the epididymis, CD30 expression was exclusively observed in luminal epithelia and some interstitial cells. Taken together, these results show a spatio-temporal regulation of CD30 expression in mouse testis and epididymis and suggest a possible role of CD30 in spermatogonia and spermatocytes.

• Journal of Gastric Cancer
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• 제11권3호
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• pp.141-145
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• 2011

Although the biological potential of gastric epithelial dysplasia (GED) as a precursor of gastric cancer has never been in doubt, the classification of these lesions has been controversial and fraught with marked variations in approach to diagnosis across the world. The complexity of cyto-architectural features has been considered to be of paramount importance for the diagnosis of carcinoma in Japan, while breach of the basement membrane and invasion into the lamina propria has been considered the sine qua non of malignancy and hence a pre-requisite for the diagnosis of cancer in the West. In Korea, although the incidence of gastric cancer is similar to Japan, the diagnostic approach to GED or cancer seems to lie midway between Western and Japanese criteria. In this review, we will discuss the difference in the diagnosis of GED and cancer between two pathologists working in the comprehensive cancer center located in Japan and Korea, one of the most prevalent areas in the world for gastric cancer.